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Quantitation in Lateral Flow Immunoassays: Self-Contained Reading to Stand Alone...
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Quantitation in Lateral Flow Immunoassays: Self-Contained Reading to Stand Alone Instruments and Cellphones

A special issue of Biosensors (ISSN 2079-6374). This special issue belongs to the section "Biosensor and Bioelectronic Devices".

Deadline for manuscript submissions: closed (31 December 2021) | Viewed by 4273

Special Issue Editors

Dr. Rodica Elena Ionescu

E-Mail Website
Guest Editor
Light, Nanomaterials, Nanotechnologies (L2n) Laboratory, CNRS UMR 7076, University of Technology of Troyes, 12 Rue Marie Curie CS 42060, 10004 Troyes, France
Interests: nanoparticles; multi-analyte biosensing platforms; pollutants toxicity to living cells
Special Issues, Collections and Topics in MDPI journals
Prof. Dr. Robert Steven Marks

E-Mail Website
Guest Editor
Department of Biotechnology Engineering, Ben Gurion University of the Negev, Beer-Sheva 8410500, Israel
Interests: biosensors; diagnostics; biomaterials; environmental toxicity; drug discovery; vaccines
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

The lateral flow immunoassay (LFIA) method is one of the most successful and versatile strategies in point-of-need applications. Among the many types of immunoassay-based tests, the lateral flow immunoassays embody the ideal technique to be used in a decentralized setting, with convenience as a testing strategy thanks to its simplicity, rapidity, cost-effectiveness, and lack of requirement for sophisticated equipment or technical expertise. The past decades have witnessed a terrific evolution in lateral flow immunoassay technologies, and lateral flow immunoassays are becoming well suited to replace laboratory-based immunoassays in point-of-care testing locations. The Special Issue, titled "Quantitation in Lateral Flow Immunoassays: Self-Contained Reading to Stand Alone Instruments and Cellphones”, regroups the various innovation attempts in order to bring more versatile features (proof-of-value) to conventional lateral flow immunoassays, such as the ability to quantitate; thus, enabling new medical niche markets to be served, including POCT at home. Quantitation can be done by creating new system configurations, adapting instrumentation to provide quantitative data on the signals produced, and finally how cellphones will enable home monitoring and their connection to the cloud/artificial intelligence/big data ecosystem.

Prof. Dr. Rodica Elena Ionescu
Prof. Dr. Robert Steven Marks
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Biosensors is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • immunoassays
  • lateral flow immunoassay
  • quantitation
  • point-of-care tests

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Published Papers (1 paper)

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Research

17 pages, 2422 KiB  
Article
Optimizing Effective Parameters to Enhance the Sensitivity of Vertical Flow Assay for Detection of Escherichia coli
by Manpreet Kaur and Evgeni Eltzov
Biosensors 2022, 12(2), 63; https://doi.org/10.3390/bios12020063 - 25 Jan 2022
Cited by 9 | Viewed by 3634
Abstract
Vertical flow immunoassays (VFIAs) are considered potential point-of-care testing (POCT) devices compared to lateral flow assays due to their ability to analyze a comparatively large sample volume and ease of multiplexing. However, VFIA devices are limited by low analytical sensitivity when coupled with [...] Read more.
Vertical flow immunoassays (VFIAs) are considered potential point-of-care testing (POCT) devices compared to lateral flow assays due to their ability to analyze a comparatively large sample volume and ease of multiplexing. However, VFIA devices are limited by low analytical sensitivity when coupled with a visual colorimetric signal. Herein, we carefully analyzed key parameters that accounted for the proper functionality of VFIA that can be modified to enhance the overall sensitivity of VFIA. In particular, we focused on improving the stability of conjugate pads impregnated with capture antibodies, maintaining a controlled flow rate to ensure higher analyte reactivity with capture antibodies, and enhancing the absorption efficiency. The results showed that air-drying of conjugate pads in the presence of 5% (w/v) lactose significantly improved the stability of antibodies during long-term storage. Integration of dissolvable polyvinyl alcohol (PVA) membrane of optimal concentration as a time-barrier film into the sensor delayed the flow of samples, thereby increasing the biorecognition interaction time between immunoreagents for the formation of immuno-complexes, which in turn led to higher sensitivity of the assay. Furthermore, the employment of an absorbent pad with higher water holding capacity significantly reduced the non-specific binding of immunocomplexes, thereby reducing the possibility of false-negative results. Full article
(This article belongs to the Special Issue Quantitation in Lateral Flow Immunoassays: Self-Contained Reading to Stand Alone Instruments and Cellphones)
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