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Biosensors
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Biosensors Based on Streptavidin
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Biosensors Based on Streptavidin

A special issue of Biosensors (ISSN 2079-6374). This special issue belongs to the section "Biosensor Materials".

Deadline for manuscript submissions: closed (31 October 2023) | Viewed by 6173

Special Issue Editors

Dr. Xinyao Yi

E-Mail Website
Guest Editor
College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China
Interests: electrochemical biosensors; surface plasmon resonance biosensors; nanomaterials
Prof. Dr. Ning Xia

E-Mail Website
Guest Editor
College of Chemistry and Chemical Engineering, Anyang Normal University, Anyang 455000, China
Interests: biosensors; redox cycling; immunosensors; DNA sensors; aptasensors
Special Issues, Collections and Topics in MDPI journals
Dr. Yuanqiang Hao

E-Mail Website
Guest Editor
Key Laboratory of Theoretical Organic Chemistry and Functional Molecule, Ministry of Education, School of Chemistry and Chemical Engineering, Hunan University of Science and Technology, Xiangtan 411201, China
Interests: fluorescent/photoelectrochmical analysis

Special Issue Information

Dear Colleague,

Streptavidin (SA) is an ~56 kDa homotetramer that can bind up to four biotin molecules with extraordinarily high affinity (Kd ≈ 10-14 M). The tetrameric conformation of streptavidin can enable the amplification of weak signals and improve detection sensitivity. The relatively small size of biotin allows for binding to active molecules. Due to the highly selective and stable interaction and the easy biotinylation of biomolecules, the streptavidin–biotin interactions have been used to develop robust and highly sensitive assays for the detection of biomarkers such as proteins and nucleic acids. Enzymes, nanomaterials, and fluorescent conjugates of streptavidin are commercally applied in enzyme-linked immunosorbent assay and other novel biosensors. This Special Issue "Biosensors Based on Streptavidin" aims to explore the applications of streptavidin–biotin interactions in bioassays, including but not limited to the synthesis of biotin derivatives, modification of solid interface, and amplification of sensing signals. We invite research and review papers that help advance the field of bio-recognition and application of streptavidin–biotin interactions for bioassays.

Dr. Xinyao Yi
Prof. Dr. Ning Xia
Dr. Yuanqiang Hao
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Biosensors is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • streptavidin
  • biosensors
  • biomarkers
  • enzymes
  • nanomaterials
  • immunoassays
  • aptasensors

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Published Papers (3 papers)

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Research

14 pages, 6118 KiB  
Article
Streptavidin-Conjugated DNA for the Boronate Affinity-Based Detection of Poly(ADP-Ribose) Polymerase-1 with Improved Sensitivity
by Fengli Gao, Gang Liu, Yishu Qiao, Xiuwen Dong and Lin Liu
Biosensors 2023, 13(7), 723; https://doi.org/10.3390/bios13070723 - 10 Jul 2023
Cited by 2 | Viewed by 1375
Abstract
This work reports the development of a fluorescence method for the detection of poly(ADP-ribose) polymerase-1 (PARP1), in which a phenylboronic acid-modified fluorescein isothiocyanate dye (FITC-PBA) was used to recognize the formed poly(ADP-ribose) (PAR) polymer. The detection system was designed by conjugating recombinant streptavidin [...] Read more.
This work reports the development of a fluorescence method for the detection of poly(ADP-ribose) polymerase-1 (PARP1), in which a phenylboronic acid-modified fluorescein isothiocyanate dye (FITC-PBA) was used to recognize the formed poly(ADP-ribose) (PAR) polymer. The detection system was designed by conjugating recombinant streptavidin (rSA) with PARP1-specific double-stranded DNA (dsDNA) through streptavidin–biotin interaction. Capture of PARP1 via rSA–biotin–dsDNA allowed for the poly-ADP-ribosylation (PARylation) of both rSA and PARP1 in a homogeneous solution. The resulting rSA–biotin–dsDNA/PAR conjugates were then captured and separated via the commercialized nitrilotriacetic acid–nickel ion-modified magnetic bead (MB-NTA-Ni) through the interaction between NTA–Ni on MB surface and oligohistidine (His6) tag in rSA. The PAR polymer could capture the dye of FITC-PBA through the borate ester interaction between the boronic acid moiety in PBA and the cis-diol group in ribose, thus causing a decrease in fluorescence signal. The PARylation of streptavidin and the influence of steric hindrance on PARylation efficiency were confirmed using reasonable detection strategies. The method showed a wide linear range (0.01~20 U) and a low detection limit (0.01 U). This work should be valuable for the development of novel biosensors for the detection of poly(ADP-ribose) polymerases and diol-containing species. Full article
(This article belongs to the Special Issue Biosensors Based on Streptavidin)
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13 pages, 2767 KiB  
Article
Hybridization Chain Reaction-Based Electrochemical Biosensors by Integrating the Advantages of Homogeneous Reaction and Heterogeneous Detection
by Ning Xia, Jiayou Cheng, Linxu Tian, Shuo Zhang, Yunqiu Wang and Gang Li
Biosensors 2023, 13(5), 543; https://doi.org/10.3390/bios13050543 - 12 May 2023
Cited by 6 | Viewed by 2226
Abstract
The conventional hybridization chain reaction (HCR)-based electrochemical biosensors usually require the immobilization of probes on the electrode surface. This will limit the applications of biosensors due to the shortcomings of complex immobilization processes and low HCR efficiency. In this work, we proposed astrategy [...] Read more.
The conventional hybridization chain reaction (HCR)-based electrochemical biosensors usually require the immobilization of probes on the electrode surface. This will limit the applications of biosensors due to the shortcomings of complex immobilization processes and low HCR efficiency. In this work, we proposed astrategy for the design of HCR-based electrochemical biosensors by integrating the advantages of homogeneous reaction and heterogeneous detection. Specifically, the targets triggered the autonomous cross-opening and hybridization oftwobiotin-labeled hairpin probes to form long-nicked dsDNA polymers. The HCR products with many biotin tags were then captured by a streptavidin-covered electrode, thus allowing for the attachment of streptavidin-conjugated signal reporters through streptavidin–biotin interactions. By employing DNA and microRNA-21 as the model targets and glucose oxidase as the signal reporter, the analytical performances of the HCR-based electrochemical biosensors were investigated. The detection limits of this method were found to be 0.6 fM and 1 fM for DNA and microRNA-21, respectively. The proposed strategy exhibited good reliability for target analysis in serum and cellular lysates. The strategy can be used to develop various HCR-based biosensors for a wide range of applications because sequence-specific oligonucleotides exhibit high binding affinity to a series of targets. In light of the high stability and commercial availability of streptavidin-modified materials, the strategy can be used for the design of different biosensors by changing the signal reporter and/or the sequence of hairpin probes. Full article
(This article belongs to the Special Issue Biosensors Based on Streptavidin)
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12 pages, 1916 KiB  
Article
Modular Set of Reagents in Lateral Flow Immunoassay: Application for Antibiotic Neomycin Detection in Honey
by Lyubov V. Barshevskaya, Dmitriy V. Sotnikov, Anatoly V. Zherdev and Boris B. Dzantiev
Biosensors 2023, 13(5), 498; https://doi.org/10.3390/bios13050498 - 25 Apr 2023
Cited by 4 | Viewed by 2021
Abstract
A scheme of modular competitive immunochromatography with an analyte-independent test strip and changeable specific immunoreactants has been proposed. Native (detected) and biotinylated antigens interact with specific antibodies during their preincubation in solution, that is, without the immobilization of reagents. After this, the detectable [...] Read more.
A scheme of modular competitive immunochromatography with an analyte-independent test strip and changeable specific immunoreactants has been proposed. Native (detected) and biotinylated antigens interact with specific antibodies during their preincubation in solution, that is, without the immobilization of reagents. After this, the detectable complexes on the test strip are formed by the use of streptavidin (which binds biotin with high affinity), anti-species antibodies, and immunoglobulin-binding streptococcal protein G. The technique was successfully applied for the detection of neomycin in honey. The visual and instrumental detection limits were 0.3 and 0.014 mg/kg, respectively, and the degree of neomycin revealed in honey samples varied from 85% to 113%. The efficiency of the modular technique with the use of the same test strip for different analytes was confirmed for streptomycin detection. The proposed approach excludes the necessity of finding the condition of immobilization for each new specific immunoreactant and transferring the assay to other analytes by a simple choice of concentrations for preincubated specific antibodies and the hapten–biotin conjugate. Full article
(This article belongs to the Special Issue Biosensors Based on Streptavidin)
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