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Biosensors
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New Technology of Nucleic Acid Detection Based Point-of-Care Diagnostics
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New Technology of Nucleic Acid Detection Based Point-of-Care Diagnostics

A special issue of Biosensors (ISSN 2079-6374). This special issue belongs to the section "Biosensors and Healthcare".

Deadline for manuscript submissions: closed (30 November 2023) | Viewed by 3344

Special Issue Editor

Dr. Manish Biyani

E-Mail Website
Guest Editor
Department of Bioscience and Biotechnology, Japan Advanced Institute of Science and Technology, Nomi 9231292, Japan
Interests: biosensors; molecular diagnostics; aptamer; DNA-coupled protein microarray; electrochemical sensor

Special Issue Information

Dear Colleagues,

On the emergence of new infectious virus strains, such as SARS-CoV-2, that result in outbreaks, the availability of rapid and nucleic acid-based testing of infected suspects (symptomatic or asymptomatic) that produces results in minutes and directly from samples is the most effective way to prevent human-to-human disease transmission and pandemics. Hundreds of molecular tests have been rapidly introduced, albeit still facing an arduous journey to practical application and mass usage, mainly due to the shortcomings of the existing laboratory-based testing paradigm for RNA-based viral diagnostics. This Special Issue “New Technology of Nucleic Acid Detection Based Point-of-Care Diagnostics” focuses on recent advances in the laboratory-free and rapid nucleic acid-based testing technologies and their practical applications toward implementation of the ‘One Health’ concept, a crucial approach to preparing for future pandemics by recognizing the interconnections and establishing early warning surveillance at the interface between humans, animals, and their shared environments.

We invite submissions of new research that guide us in advancing nucleic acid-based point-of-care testing to improve diagnostic preparedness.

Topics of interest include but are not limited to the following:

  • Simple and lab-free sampling for onsite nucleic acid testing
  • Sensitive and reliable nucleic acid amplification technology
  • Portable and cost-effective biosensing devices
  • Point-of-care protocols for onsite nucleic acid testing

Dr. Manish Biyani
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Biosensors is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Published Papers (2 papers)

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Research

13 pages, 306 KiB  
Article
Rapid On-Site Detection of Arboviruses by a Direct RT-qPCR Assay
by Moufid Mhamadi, Giulia Mencattelli, Alioune Gaye, El Hadji Ndiaye, Aïssatou Aïcha Sow, Martin Faye, Marie Henriette Dior Ndione, Moussa Moïse Diagne, Moundhir Mhamadi, Ousmane Faye, Manfred Weidmann, Oumar Faye, Mawlouth Diallo and Cheikh Tidiane Diagne
Biosensors 2023, 13(12), 1035; https://doi.org/10.3390/bios13121035 - 16 Dec 2023
Viewed by 1708
Abstract
Arthropod-borne diseases currently constitute a source of major health concerns worldwide. They account for about 50% of global infectious diseases and cause nearly 700,000 deaths every year. Their rapid increase and spread constitute a huge challenge for public health, highlighting the need for [...] Read more.
Arthropod-borne diseases currently constitute a source of major health concerns worldwide. They account for about 50% of global infectious diseases and cause nearly 700,000 deaths every year. Their rapid increase and spread constitute a huge challenge for public health, highlighting the need for early detection during epidemics, to curtail the virus spread, and to enhance outbreak management. Here, we compared a standard quantitative polymerase chain reaction (RT-qPCR) and a direct RT-qPCR assay for the detection of Zika (ZIKV), Chikungunya (CHIKV), and Rift Valley Fever (RVFV) viruses from experimentally infected-mosquitoes. The direct RT-qPCR could be completed within 1.5 h and required 1 µL of viral supernatant from homogenized mosquito body pools. Results showed that the direct RT-qPCR can detect 85.71%, 89%, and 100% of CHIKV, RVFV, and ZIKV samples by direct amplifications compared to the standard method. The use of 1:10 diluted supernatant is suggested for CHIKV and RVFV direct RT-qPCR. Despite a slight drop in sensitivity for direct PCR, our technique is more affordable, less time-consuming, and provides a better option for qualitative field diagnosis during outbreak management. It represents an alternative when extraction and purification steps are not possible because of insufficient sample volume or biosecurity issues. Full article
(This article belongs to the Special Issue New Technology of Nucleic Acid Detection Based Point-of-Care Diagnostics)
13 pages, 2218 KiB  
Article
Rapid and Highly Sensitive Detection of Leishmania by Combining Recombinase Polymerase Amplification and Solution-Processed Oxide Thin-Film Transistor Technology
by Weidong Wu, Manish Biyani, Daisuke Hirose and Yuzuru Takamura
Biosensors 2023, 13(8), 765; https://doi.org/10.3390/bios13080765 - 28 Jul 2023
Viewed by 1065
Abstract
Nucleic acid detection is widely used to identify infectious diseases and ensure food safety. However, conventional PCR-based techniques are time consuming. Thus, this study aims to combine recombinase polymerase amplification (RPA), which enables the rapid amplification of even trace amounts of nucleic acid [...] Read more.
Nucleic acid detection is widely used to identify infectious diseases and ensure food safety. However, conventional PCR-based techniques are time consuming. Thus, this study aims to combine recombinase polymerase amplification (RPA), which enables the rapid amplification of even trace amounts of nucleic acid fragments within 10–40 min at 37–42 °C, and solution-processed oxide thin-film transistor (TFT) technology, which exhibits high detection sensitivity, to detect Leishmania. A single-stranded anti-probe was incorporated into the RPA primer to facilitate effective hybridization between the RPA product and the immobilized probe on the solution-processed oxide TFT. The RPA-amplified product carrying an anti-probe enabled specific binding to the chip surface. Changes in current were monitored before and after sample incubation to identify the target nucleic acids in the samples accurately. The proposed method achieved a remarkable limit of detection of 101 copies/μL of the Leishmania HSP70 fragment within 30 min. The design of the probes on the solution-processed oxide TFT surface and the anti-probe simplified the detection of other target nucleic acids, eliminating the need to denature DNA double-strands for specific binding during nucleic acid detection. Thus, the novel method offers the advantage of requiring minimal reagent resources and eliminates the need for complex procedures. Full article
(This article belongs to the Special Issue New Technology of Nucleic Acid Detection Based Point-of-Care Diagnostics)
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