Surface Plasmon Resonance (SPR)-Based Sensors

A special issue of Biosensors (ISSN 2079-6374). This special issue belongs to the section "Optical and Photonic Biosensors".

Deadline for manuscript submissions: closed (30 July 2021) | Viewed by 15844

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Guest Editor
Biomedical Research Institute, Central 6, National Institute of Advanced Industrial Science and Technology, 1-1-1 Higashi, Tsukuba City, Ibaraki 305-8566, Japan
Interests: ribozymes; RNA-protein interactions; 3D structures of RNA-protein complexes; aptamers; aptamer-based biosensors; molecular beacon aptamers; anti-viral aptamers
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Special Issue Information

Dear Colleagues,

Surface Plasmon Resonance (SPR) has been used to analyze a wide range of biomolecular interactions, including protein–protein, DNA–protein, RNA–protein, antigen–antibody, lipid membrane–protein, protein–glycan or polysaccharide, protein–small ligand, DNA–small ligand, RNA–small ligand, receptor–drug, and receptor–virus interactions. SPR enables label-free detection, which makes it possible to monitor complexes in a real-time fashion, and facilitates the isolation of complexes if necessary. SPR-based biomolecular analyses require only a small amount of sample to characterize the binding efficiency between the two ligands for the purpose of determining the rate constants. Although the analysis of biomolecular interactions is carried out routinely using SPR in batch mode, in recent years most biomolecular analyses have been routinely carried out in a high-throughput fashion that includes drug discovery.

Considering the recent developments in SPR applications, we plan to publish a Special Issue entitled “Surface Plasmon Resonance (SPR)-Based Sensors”. For this Special Issue, we invite authors to submit manuscripts that help to advance SPR methods and applications in various fields, including drug discovery, quality analysis, evaluation of macromolecular interactions, and high-throughput analyses of biomolecular interactions.

Dr. Penmetcha Kumar
Guest Editor

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Keywords

  • SPR
  • biomolecular interactions
  • drug discovery
  • diagnosis

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Published Papers (2 papers)

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Research

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13 pages, 4072 KiB  
Article
Comparison of Single- and Mixed-Sized Gold Nanoparticles on Lateral Flow Assay for Albumin Detection
by Sasima Chotithammakul, Michael B. Cortie and Dakrong Pissuwan
Biosensors 2021, 11(7), 209; https://doi.org/10.3390/bios11070209 - 26 Jun 2021
Cited by 19 | Viewed by 4047
Abstract
The sensitivity and reproducibility of the lateral flow assay can be influenced by multiple factors, such as the size of gold nanoparticles (GNPs) employed. Here, we evaluated the analytical performance of single-sized and mixed-sized GNPs using a simple lateral flow assay (LFA) platform. [...] Read more.
The sensitivity and reproducibility of the lateral flow assay can be influenced by multiple factors, such as the size of gold nanoparticles (GNPs) employed. Here, we evaluated the analytical performance of single-sized and mixed-sized GNPs using a simple lateral flow assay (LFA) platform. This platform was used as a model assay to diagnose albumin levels and demonstrate the analytical performance of single-sized and mixed-sized GNPs in LFA tests. Two sizes of GNPs@anti-bovine serum albumin (BSA) conjugate proteins were mixed at different ratios. The unique optical properties of the GNPs induced a distinguishing color-shedding effect on the single- and mixed-sized GNPs@anti-BSA conjugates interacting with the target analyte BSA spotted on the test line. The use of mixed-sized GNPs@anti-BSA conjugates enhanced signal relative to the 20 nm GNPs, and provided superior stability compared with solely employing the large GNPs (50 nm). The proposed platform in this study could provide an efficient BSA detection mechanism that can be utilized as a model biomarker for confronting chronic kidney disease. Full article
(This article belongs to the Special Issue Surface Plasmon Resonance (SPR)-Based Sensors)
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Review

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18 pages, 4551 KiB  
Review
Surface Plasmon Resonance (SPR)- and Localized SPR (LSPR)-Based Virus Sensing Systems: Optical Vibration of Nano- and Micro-Metallic Materials for the Development of Next-Generation Virus Detection Technology
by Kenshin Takemura
Biosensors 2021, 11(8), 250; https://doi.org/10.3390/bios11080250 - 26 Jul 2021
Cited by 73 | Viewed by 11161
Abstract
The global damage that a widespread viral infection can cause is evident from the ongoing COVID-19 pandemic. The importance of virus detection to prevent the spread of viruses has been reaffirmed by the pandemic and the associated social and economic damage. Surface plasmon [...] Read more.
The global damage that a widespread viral infection can cause is evident from the ongoing COVID-19 pandemic. The importance of virus detection to prevent the spread of viruses has been reaffirmed by the pandemic and the associated social and economic damage. Surface plasmon resonance (SPR) in microscale and localized SPR (LSPR) in nanoscale virus sensing systems are thought to be useful as next-generation detection methods. Many studies have been conducted on ultra-sensitive technologies, especially those based on signal amplification. In some cases, it has been reported that even a low viral load can be measured, indicating that the virus can be detected in patients even in the early stages of the viral infection. These findings corroborate that SPR and LSPR are effective in minimizing false-positives and false-negatives that are prevalent in the existing virus detection techniques. In this review, the methods and signal responses of SPR and LSPR-based virus detection technologies are summarized. Furthermore, this review surveys some of the recent developments reported and discusses the limitations of SPR and LSPR-based virus detection as the next-generation detection technologies. Full article
(This article belongs to the Special Issue Surface Plasmon Resonance (SPR)-Based Sensors)
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