A Revolutionary Biosensing Platform for Biomedicine: Cells, Tissues and Organs On-Chip

A special issue of Biosensors (ISSN 2079-6374). This special issue belongs to the section "Biosensor and Bioelectronic Devices".

Deadline for manuscript submissions: closed (30 September 2023) | Viewed by 3625

Special Issue Editors


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Guest Editor
Future Industries Institute, University of South Australia, Mawson Lakes Campus, Adelaide, SA 5095, Australia
Interests: biomaterials; biosensing; microfluidics and organ-on-a-chip model
Special Issues, Collections and Topics in MDPI journals

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Guest Editor
Department of Chemical and Materials Engineering, National Central University, Taoyuan, Taiwan
Interests: plasmonic biosensors; functional biointerfaces; antifouling materials; zwitterionic materials
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Over the past few decades, huge advancements in the field of biomedical engineering have fostered significant progress in the development of cell culture systems which can better recapitulate the biological and physiological environment of the human body. However, the monitoring of cellular/tissue events in these systems relies heavily on either laborious bioassay measurements and/or end-point readouts such as immunohistochemical staining, which fails to respond to the external stimuli in non-invasive and in situ manners. To this end, there is an unmet need to integrate biosensing technologies with cell culture systems to provide substantial cellular and molecular biology insights in real time.

We anticipate that cutting-edge biosensing technologies that are compatible with advanced cells, tissues, and organs-on-chip culture systems will potentially enhance their performance in diagnostics and personalized therapeutics by providing unprecedented swathes of biosensing data.

This Special Issue welcomes high-quality publications including original research articles and reviews related to recent advances in biosensing technology for cells, tissues, and organs-on-chip applications.

Research areas may include the following:

  • Electrochemical biosensors
  • Optical biosensors
  • Microfluidics
  • Organs-on-chip
  • Physical biosensors

We look forward to receiving your contributions.

Dr. Chih-Tsung Yang
Prof. Dr. Chun-Jen Huang
Guest Editors

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Published Papers (1 paper)

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Research

22 pages, 2874 KiB  
Article
New Flow Cytometric Methods for Monitoring STAT5 Signaling Reveal Responses to SARS-CoV-2 Antigen-Specific Stimulation in FOXP3+ Regulatory T Cells also in Patients with Advanced Chronic Lymphocytic Leukemia
by Zlatko Roškar, Mojca Dreisinger, Primož Tič, Evgenija Homšak, Sebastjan Bevc and Aleš Goropevšek
Biosensors 2023, 13(5), 539; https://doi.org/10.3390/bios13050539 - 11 May 2023
Cited by 1 | Viewed by 2834
Abstract
Increased frequency of CD4+CD25+ regulatory T-cells (Treg) has been associated with disease progression in chronic lymphocytic leukemia (CLL). Flow cytometric methods, which allow for the simultaneous analysis of their specific transcription factor Foxp3 and activated STAT proteins, together with proliferation [...] Read more.
Increased frequency of CD4+CD25+ regulatory T-cells (Treg) has been associated with disease progression in chronic lymphocytic leukemia (CLL). Flow cytometric methods, which allow for the simultaneous analysis of their specific transcription factor Foxp3 and activated STAT proteins, together with proliferation can help to elucidate the signaling mechanisms driving Treg expansion and suppression of FOXP3- conventional CD4+T-cells (Tcon). Herein, we first report a novel approach in which STAT5 phosphorylation (pSTAT5) and proliferation (BrdU-FITC incorporation) could be analyzed specifically in FOXP3+ and FOXP3- responding cells after CD3/CD28 stimulation. The addition of magnetically purified CD4+CD25+ T-cells from healthy donors to cocultured autologous CD4+CD25 T-cells resulted in suppression of Tcon cell cycle progression accompanied by a decrease in pSTAT5. Next, a method using imaging flow cytometry is presented for the detection of cytokine-dependent pSTAT5 nuclear translocation in FOXP3-expressing cells. Finally, we discuss our experimental data obtained by combining Treg pSTAT5 analysis and antigen-specific stimulation with SARS-CoV-2 antigens. Applying these methods on samples from patients revealed Treg responses to antigen-specific stimulation and significantly higher basal pSTAT5 in CLL patients treated with immunochemotherapy. Thus, we speculate that through the use of this pharmacodynamic tool, the efficacy of immunosuppressive drugs and their possible off-target effects can be assessed. Full article
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