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Biosensors
Special Issues
Advanced Optical Methods for Biosensing
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Advanced Optical Methods for Biosensing

A special issue of Biosensors (ISSN 2079-6374). This special issue belongs to the section "Optical and Photonic Biosensors".

Deadline for manuscript submissions: closed (20 January 2025) | Viewed by 4805

Special Issue Editors

Dr. Pinkie Jacob Eravuchira

E-Mail Website
Guest Editor
Environment and People: A Centre for Scientific Research and Innovation, Kerala 670645, India
Interests: biosensors; optical spectroscopy; microfluidics
Special Issues, Collections and Topics in MDPI journals
Dr. Chih-Tsung Yang

E-Mail Website
Guest Editor
Future Industries Institute, University of South Australia, Mawson Lakes Campus, Adelaide, SA 5095, Australia
Interests: biomaterials; biosensing; microfluidics and organ-on-a-chip model
Special Issues, Collections and Topics in MDPI journals
Prof. Dr. Nathan Lindquist

E-Mail Website
Guest Editor
Physics Department, Bethel University, St. Paul, MN 55112, USA
Interests: nano-photonics and plasmonics; super-resolution imaging and microscopy; digital holographic microscopy; optical biosensing; spectroscopy; nanofabrication
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Biosensors are analytical tools that facilitate the accurate diagnosis and analysis of biological states and diseases in living systems. These diagnostic devices detect and quantitatively measure biological and pathological analytes in order to aid a better understanding of the biological processes and changes in cells and tissues. Much innovative research is being carried out to create high-accuracy and cost-effective optical biosensors that can help with disease diagnoses. New advancements, especially in the field of optical methods for biosensing, are essential as these biological detection and imaging tools find a wide range of potential applications in healthcare, medicine, and more.

In this Special Issue of Biosensors, we invite you to contribute high-quality research on Advanced Optical Methods for Biosensing and Bioimaging. Contributions that cover both experimental and theoretical work on the latest developments in the field of optical biosensors will be considered. Original research papers, communication, research notes, and comprehensive review articles are welcome. Manuscripts will be internationally peer-reviewed.

Dr. Pinkie Jacob Eravuchira
Dr. Chih-Tsung Yang
Prof. Dr. Nathan Lindquist
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Biosensors is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • optical biosensors
  • lab-on-a-chip
  • point-care devices
  • mi-crofluidics

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Further information on MDPI's Special Issue polices can be found here.

Published Papers (5 papers)

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Research

12 pages, 912 KiB  
Article
On-Chip Polarization Light Microscopy
by Túlio de L. Pedrosa, Renato E. de Araujo and Sebastian Wachsmann-Hogiu
Biosensors 2025, 15(2), 79; https://doi.org/10.3390/bios15020079 - 30 Jan 2025
Viewed by 274
Abstract
Polarization light microscopy (PLM) enables detailed examination of birefringent materials and reveals unique features that cannot be observed under non-polarized light. Implementation of this technique for quantitative PLM (QPLM) assessment of samples is challenging and requires specialized components and equipment. Here, we demonstrate [...] Read more.
Polarization light microscopy (PLM) enables detailed examination of birefringent materials and reveals unique features that cannot be observed under non-polarized light. Implementation of this technique for quantitative PLM (QPLM) assessment of samples is challenging and requires specialized components and equipment. Here, we demonstrate QPLM on a semiconductor imaging chip that is suitable for point-of-care/need applications. A white LED illumination was used with crossed polarizers and a full wave plate to perform on-chip, non-contact-mode QPLM. Polarization complexity is probed by assessing the multispectral phase shift experienced by white light through the distinct optical paths of the sample. This platform can achieve micrometer-scale spatial resolution with a Field of View determined by the size of the semiconductor sensor. Visualization of a biological sample (Euglena gracilis) was demonstrated, as well as the detection of Monosodium Urate crystals, where the presence of negative birefringence of crystals in synovial fluid is important for the diagnosis of gout. Full article
(This article belongs to the Special Issue Advanced Optical Methods for Biosensing)
24 pages, 5350 KiB  
Article
SPR Biosensor Based on Bilayer MoS2 for SARS-CoV-2 Sensing
by Talia Tene, Stefano Bellucci and Cristian Vacacela Gomez
Biosensors 2025, 15(1), 21; https://doi.org/10.3390/bios15010021 - 4 Jan 2025
Viewed by 773
Abstract
The COVID-19 pandemic has highlighted the urgent need for rapid, sensitive, and reliable diagnostic tools for detecting SARS-CoV-2. In this study, we developed and optimized a surface plasmon resonance (SPR) biosensor incorporating advanced materials to enhance its sensitivity and specificity. Key parameters, including [...] Read more.
The COVID-19 pandemic has highlighted the urgent need for rapid, sensitive, and reliable diagnostic tools for detecting SARS-CoV-2. In this study, we developed and optimized a surface plasmon resonance (SPR) biosensor incorporating advanced materials to enhance its sensitivity and specificity. Key parameters, including the thickness of the silver layer, silicon nitride dielectric layer, molybdenum disulfide (MoS2) layers, and ssDNA recognition layer, were systematically optimized to achieve the best balance between sensitivity, resolution, and attenuation. The optimized configuration, consisting of a 45 nm silver layer, a 13 nm silicon nitride layer, 2 MoS2 layers, and a 5 nm ssDNA layer, demonstrated superior performance for detecting SARS-CoV-2 in PBS solution. The biosensor exhibited high sensitivity at low viral concentrations, achieving a sensitivity of 375.01°/RIU, a detection accuracy of 0.002, and a quality factor of 38.34 at 1.0 mM SARS-CoV-2 concentration. Performance metrics validated the sensor’s capability for reliable detection, particularly in early-stage diagnostics where timely intervention is critical. Moreover, the biosensor’s linear response to refractive index changes confirmed its potential for quantitative viral concentration analysis. This study underlines the significance of integrating advanced materials, such as MoS2 and silicon nitride, to enhance SPR biosensor performance. The findings establish the proposed biosensor as a robust and precise diagnostic tool for SARS-CoV-2 detection, with potential applications in clinical diagnostics and epidemiological monitoring. Full article
(This article belongs to the Special Issue Advanced Optical Methods for Biosensing)
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12 pages, 6188 KiB  
Article
Bi-Plane Multicolor Scanning Illumination Microscopy with Multispot Excitation and a Distorted Diffraction Grating
by Siwei Li, Yunke Zhang, Zhiwen Liao, Zengyuan Tian, Hairulazwan Hashim, Youjun Zeng and Yandong Zhang
Biosensors 2024, 14(11), 550; https://doi.org/10.3390/bios14110550 - 13 Nov 2024
Viewed by 806
Abstract
Multifocus microscopy has previously been demonstrated to provide volumetric information from a single shot. However, the practical application of this method is challenging due to its weak optical sectioning and limited spatial resolution. Here, we report on the combination of a distorted diffraction [...] Read more.
Multifocus microscopy has previously been demonstrated to provide volumetric information from a single shot. However, the practical application of this method is challenging due to its weak optical sectioning and limited spatial resolution. Here, we report on the combination of a distorted diffraction grating and multifocal scanning illumination microscopy to improve spatial resolution and contrast. DG is introduced in the emission path of the multifocal scanning illumination microscopy, which splits the fluorescence signal from different sample layers into different diffraction orders. After postprocessing, super-resolution wide-field images of different sample layers can be reconstructed from single 2D scanning. Full article
(This article belongs to the Special Issue Advanced Optical Methods for Biosensing)
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12 pages, 3019 KiB  
Article
Analysis of Random Lasing in Human Blood
by Sergio de Armas-Rillo, Beatriz Abdul-Jalbar, Josmar Salas-Hernández, Jose María Raya-Sánchez, Tomás González-Hernández and Fernando Lahoz
Biosensors 2024, 14(9), 441; https://doi.org/10.3390/bios14090441 - 13 Sep 2024
Viewed by 998
Abstract
Random lasing (RL) is an optical phenomenon that arises from the combination of light amplification with optical feedback through multiple scattering events. In this paper, we present our investigations of RL generation from human blood samples. We tested mixtures of rhodamine B dye [...] Read more.
Random lasing (RL) is an optical phenomenon that arises from the combination of light amplification with optical feedback through multiple scattering events. In this paper, we present our investigations of RL generation from human blood samples. We tested mixtures of rhodamine B dye solutions with different blood components, including platelets, lymphocytes, erythrocytes, and whole blood. Intense coherent RL was obtained in all cases at relatively low pump thresholds, except for erythrocytes. We also studied the potential of RL signal analysis for biosensing applications using blood samples from healthy individuals and patients suffering from Chronic Lymphocytic Leukemia (CLL). CLL is a blood disease characterized by a high count of lymphocytes with significant morphological changes. A statistical analysis of the RL spectra based on principal component and linear discriminant analyses was conducted for classification purposes. RL-based sample discrimination was conducted for whole blood, platelet, and lymphocyte samples, being especially successful (86.7%) for the latter. Our results highlight the potential of RL analysis as a sensing tool in blood. Full article
(This article belongs to the Special Issue Advanced Optical Methods for Biosensing)
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12 pages, 1037 KiB  
Article
Brillouin Biosensing of Viscoelasticity across Phase Transitions in Ovine Cornea
by Chingis Kharmyssov and Zhandos Utegulov
Biosensors 2024, 14(8), 371; https://doi.org/10.3390/bios14080371 - 30 Jul 2024
Cited by 1 | Viewed by 1444
Abstract
Noninvasive in situ monitoring of viscoelastic characteristics of corneal tissue at elevated temperatures is pivotal for mechanical property-informed refractive surgery techniques, including thermokeratoplasty and photorefractive keratectomy, requiring precise thermal modifications of the corneal structure during these surgical procedures. This study harnesses Brillouin light [...] Read more.
Noninvasive in situ monitoring of viscoelastic characteristics of corneal tissue at elevated temperatures is pivotal for mechanical property-informed refractive surgery techniques, including thermokeratoplasty and photorefractive keratectomy, requiring precise thermal modifications of the corneal structure during these surgical procedures. This study harnesses Brillouin light scattering spectroscopy as a biosensing platform to noninvasively probe the viscoelastic properties of ovine corneas across a temperature range of 25–64 °C. By submerging the tissue samples in silicone oil, consistent hydration and immiscibility are maintained, allowing for their accurate sensing of temperature-dependent mechanical behaviors. We identify significant phase transitions in the corneal tissue, particularly beyond 40 °C, likely due to collagen unfolding, marking the beginning of thermal destabilization. A subsequent transition, observed beyond 60 °C, correlates with collagen denaturation. These phase transformations highlight the cornea’s sensitivity to both physiologically reversible and irreversible viscoelastic changes induced by mild to high temperatures. Our findings underscore the potential of the Brillouin biosensing technique for real-time diagnostics of corneal biomechanics during refractive surgeries to attain optimized therapeutic outcomes. Full article
(This article belongs to the Special Issue Advanced Optical Methods for Biosensing)
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