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Catalysts
Special Issues
Emerging Technologies and Novel Approaches for Enzymatic Catalysis
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Emerging Technologies and Novel Approaches for Enzymatic Catalysis

A special issue of Catalysts (ISSN 2073-4344). This special issue belongs to the section "Biocatalysis".

Deadline for manuscript submissions: closed (31 May 2021) | Viewed by 5490

Special Issue Editors

Prof. Dr. Kyung-Min Park

E-Mail Website
Guest Editor
Department of Food Science and Biotechnology, Wonkwang University, Iksan 54538, Korea
Interests: biocatalysts; enzyme kinetics; enzymatic bioconversions; multifunctional food additives; ecofriendly bioreactor
Dr. Yoonjee Chang

E-Mail Website
Guest Editor
Department of Food and Nutrition, Kookmin University, Seoul 02707, Korea
Interests: food safety; bacteriophage; endolysin; active packaging; edible coating; film; antimicrobial; microorganism; antiviral
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Driven by the need for the development and application of various biotechnological products, emerging technologies and novel approaches for catalysis are being attempted today. Furthermore, there has been much attention on the safety of bioprocesses and efficiency of bioconversions. In this regard, investigations on enzymatic catalysis are a promising research field. This Special Issue aims to focus on the enzymatic synthesis of various biomaterials; kinetics and bioreactors for enzymatic bioconversion; investigations on enzymatic mechanisms; the treatment of free or immobilized enzymes to control of microorganisms; novel utilizations of biocatalysts; and others. In addition, focusing on the development and application of eco-friendly catalysis is welcome. This Special Issue will not only include basic science but also applied research. It will attempt to cover review articles as well as original research papers on these topics, demonstrating both technical advances and practical utilizations.

Prof. Dr. Kyung-Min Park
Dr. Yoonjee Chang
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Catalysts is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • biocatalysts
  • nanocatalysts
  • enzymatic bioconversions
  • enzyme kinetics
  • bioprocess safety
  • food enzymology
  • microorganisms
  • bioreactor

Published Papers (2 papers)

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Research

12 pages, 1047 KiB  
Article
Comparative Analysis of Universal Protein Extraction Methodologies for Screening of Lipase Activity from Agricultural Products
by Jisu Ha, Jun-Young Park, Yoonseok Choi, Pahn-Shick Chang and Kyung-Min Park
Catalysts 2021, 11(7), 816; https://doi.org/10.3390/catal11070816 - 4 Jul 2021
Cited by 2 | Viewed by 2936
Abstract
Protein extraction techniques are absolutely required for the research of biological catalysts. The present study compared four universal protein extraction methodologies (ammonium sulfate precipitation, TCA/acetone precipitation, and two commercial kits) to provide practical information on protein extraction in order to discover a novel [...] Read more.
Protein extraction techniques are absolutely required for the research of biological catalysts. The present study compared four universal protein extraction methodologies (ammonium sulfate precipitation, TCA/acetone precipitation, and two commercial kits) to provide practical information on protein extraction in order to discover a novel lipase in agricultural products. Yields of protein extraction from 24 domestic agricultural products and their specific activities were evaluated and compared with each other. TCA/acetone precipitation showed a relatively higher extraction yield (on average, 3.41 ± 1.08 mg protein/0.1 g sample) in crude protein extraction, whereas the Pierce™ Plant Total Protein Extraction Kit showed the highest specific lipase activity on average in both spectrophotometric (266.61 ± 235.78 μU/mg protein) and fluorometric (41.52 ± 32.63 μU/mg protein) assays. Our results suggest that commercial kits for the rapid extraction of soluble functional proteins would be a better choice than conventional precipitation techniques to perform the high-throughput screening of enzyme activity from plant sources. Finally, several agricultural products such as cordyceps, pepper, bracken, and hemp, all of which exhibited an excellent specific lipase activity, were proposed as promising candidates for a source of novel lipases. Full article
(This article belongs to the Special Issue Emerging Technologies and Novel Approaches for Enzymatic Catalysis)
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12 pages, 3065 KiB  
Article
Liquid-Phase and Ultrahigh-Frequency-Acoustofluidics-Based Solid-Phase Synthesis of Biotin-Tagged 6′/3′-Sialyl-N-Acetylglucosamine by Sequential One-Pot Multienzyme System
by Mengge Gong, Tiechuan Li, Lina Wu, Zhenxing Zhang, Lishi Ren, Xuexin Duan, Hongzhi Cao, Meishan Pei, Jian-Jun Li and Yuguang Du
Catalysts 2020, 10(11), 1347; https://doi.org/10.3390/catal10111347 - 19 Nov 2020
Cited by 3 | Viewed by 2051
Abstract
6′/3′-Sialylated N-acetyllactosamine (6′/3′-SLN) is important for discrimination of the source (human or avian) of influenza virus strains. Biotinylated oligosaccharides have been widely used for analysis and quick detection. The development of efficient strategies to synthesize biotin-tagged 6′/3′-SLN have become necessary. Effective mixing [...] Read more.
6′/3′-Sialylated N-acetyllactosamine (6′/3′-SLN) is important for discrimination of the source (human or avian) of influenza virus strains. Biotinylated oligosaccharides have been widely used for analysis and quick detection. The development of efficient strategies to synthesize biotin-tagged 6′/3′-SLN have become necessary. Effective mixing is essential for enzymatic solid-phase oligosaccharide synthesis (SPOS). In the current study, newly developed technology ultrahigh-frequency-acoustofluidics (UHFA), which can provide a powerful source for efficient microfluidic mixing, solid-phase oligosaccharide synthesis and one-pot multienzyme (OPME) system, were used to develop a new strategy for oligosaccharide synthesis. Firstly, biotinylated N-acetylglucosamine was designed and chemically synthesized through traditional approaches. Secondly, biotinylated 6′- and 3′-sialyl-N-acetylglucosamines were prepared in solution through two sequential OPME modules in with a yield of ~95%. Thirdly, 6′-SLN was also prepared through UHFA-based enzymatic solid-phase synthesis on magnetic beads with a yield of 64.4%. The current strategy would be potentially used for synthesis of functional oligosaccharides. Full article
(This article belongs to the Special Issue Emerging Technologies and Novel Approaches for Enzymatic Catalysis)
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