Microfluidics and Miniaturized Systems Aiding Studies Involving Cells and Animal Models

A special issue of Cells (ISSN 2073-4409). This special issue belongs to the section "Cellular Biophysics".

Deadline for manuscript submissions: closed (15 November 2021) | Viewed by 3587

Special Issue Editor


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Guest Editor
Department of Chemistry, The University of Kansas, Lawrence, KS 66047, USA
Interests: microfluidics; liquid biopsy markers; exosomes; circulating tumor cells; cfDNA; leukemia; epithelial cancers

Special Issue Information

Dear Colleagues,

Studies involving animals and cells are essential components of research, providing vital data related to disease modelling and depiction, treatment response, disease staging, pharmacokinetics, and biophysical and molecular characterization of cells. These studies are highly relevant to the understanding of maladies and the development of potential treatments or disease management protocols. Microfluidics aid these studies by creating an environment closely resembling physiological and biophysical conditions, and providing an opportunity to design in vivo modelling for testing.

We invite experts working on the development of microscale devices and microfluidic miniaturized systems for screening tests, selection assays, cultivation, cell characterization, and pharmacokinetics studies to participate in this Special Issue of Cells. Original research articles, reviews, or short perspective articles related to microfluidics and microfluidic miniaturized systems assisting research involving biological cells, model organism, and animals are welcome. Relevant topics include, but are not limited to, monitoring neurological changes in animal brains, cellular differentiation in model organisms, disease models on a chip, cell cultivation and co-cultivation on a chip, pharmacokinetic analysis using microfluidics, drug discovery and testing, microfluidics for artificial blood vessel characterization, monitoring of cell stress in microfluidics, isolation of rare cells or immune cells, and fluidic systems for real time cell isolation and enumeration. 

Dr. Malgorzata A. Witek
Guest Editor

Manuscript Submission Information

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Keywords

  • Monitoring of neurological changes in animal studies
  • Disease models on a chip
  • Cell cultivation and co-cultivation on a chip
  • Pharmacokinetic analysis and drug discovery
  • Microfluidics for artificial blood vessels design
  • Monitoring cells stress in microfluidics
  • Isolation of rare cells
  • Cell migration in diseases

Published Papers (1 paper)

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Research

11 pages, 3592 KiB  
Article
Microfluidic Characterization of Red Blood Cells Microcirculation under Oxidative Stress
by Nadezhda A. Besedina, Elisaveta A. Skverchinskaya, Alexander S. Ivanov, Konstantin P. Kotlyar, Ivan A. Morozov, Nikita A. Filatov, Igor V. Mindukshev and Anton S. Bukatin
Cells 2021, 10(12), 3552; https://doi.org/10.3390/cells10123552 - 16 Dec 2021
Cited by 8 | Viewed by 2876
Abstract
Microcirculation is one of the basic functional processes where the main gas exchange between red blood cells (RBCs) and surrounding tissues occurs. It is greatly influenced by the shape and deformability of RBCs, which can be affected by oxidative stress induced by different [...] Read more.
Microcirculation is one of the basic functional processes where the main gas exchange between red blood cells (RBCs) and surrounding tissues occurs. It is greatly influenced by the shape and deformability of RBCs, which can be affected by oxidative stress induced by different drugs and diseases leading to anemia. Here we investigated how in vitro microfluidic characterization of RBCs transit velocity in microcapillaries can indicate cells damage and its correlation with clinical hematological analysis. For this purpose, we compared an SU-8 mold with an Si-etched mold for fabrication of PDMS microfluidic devices and quantitatively figured out that oxidative stress induced by tert-Butyl hydroperoxide splits all RBCs into two subpopulations of normal and slow cells according to their transit velocity. Obtained results agree with the hematological analysis showing that such changes in RBCs velocities are due to violations of shape, volume, and increased heterogeneity of the cells. These data show that characterization of RBCs transport in microfluidic devices can directly reveal violations of microcirculation caused by oxidative stress. Therefore, it can be used for characterization of the ability of RBCs to move in microcapillaries, estimating possible side effects of cancer chemotherapy, and predicting the risk of anemia. Full article
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