Diagnostic Methods in Forensic Pathology, Third Edition

A special issue of Diagnostics (ISSN 2075-4418). This special issue belongs to the section "Pathology and Molecular Diagnostics".

Deadline for manuscript submissions: 31 May 2025 | Viewed by 725

Special Issue Editors


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Guest Editor
Legal Medicine Section, Department of Biomedical and Dental Sciences and Morphofunctional Imaging, University of Messina, Via Consolare Valeria, 1, 98125 Messina, Italy
Interests: forensic pathology; forensic odontology, forensic microbiology; forensic radiology; virtopsy; medical liability

E-Mail Website
Guest Editor
Legal Medicine SectionDepartment of Biomedical and Dental Sciences and Morphofunctional Imaging, University of Messina, Via Consolare Valeria, 1, 98125 Messina, Italy
Interests: forensic pathology; forensic immunohistochemistry; virtopsy; legal medicine; sudden cardiac death; criminology; violence and abuse; clinical risk management; medical liability
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Special Issue Information

Dear Colleagues,

We are pleased to announce the launch of a renewed edition of the Special Issue entitled “Diagnostic Methods in Forensic Pathology”, which comprises a collection of high-quality papers related to all aspects of forensic pathology. Understanding the cause of death in forensic investigations can be complex, and autopsies alone may not provide conclusive results, making the assessment of the circumstances leading to death challenging. Additional issues in forensic pathology can include cause of death in particular circumstances (sudden death, putrefied or altered bodies), post-mortem interval (PMI) estimation and injury vitality. In many cases, forensic evaluations require a multidisciplinary approach where the forensic pathologist collaborates with other specialists, such as toxicologists, anthropologists, odontologists, radiologists, entomologists, geneticists, and engineers. This collaboration has enabled innovations in forensic practices and the development of new diagnostic strategies and techniques.This Special Issue aims to include scientific advances and perspectives for the developments and discoveries in all fields of post-mortem forensic diagnostics. To this scope, this Special Issue will include scientific articles (case report and case series with well-documented circumstantial data encompassing the application of multidisciplinary approaches, reviews and original articles), on all the forensic methods/investigations/analyses useful to reach a clear post-mortem diagnosis.

Dr. Gennaro Baldino
Dr. Elvira Ventura Spagnolo
Guest Editors

Manuscript Submission Information

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Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Diagnostics is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2600 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • multidisciplinary approach
  • forensic pathology
  • sudden/unexpected death
  • sepsis and health infections
  • suicide
  • gender violence
  • post-mortem immunohistochemistry
  • post-mortem CT
  • virtopsy

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Published Papers (1 paper)

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Research

17 pages, 3887 KiB  
Article
Decoding Time of Death: Histopathological Dynamics of Intervertebral Discs as a Novel Marker for Postmortem Interval Estimation
by Selcuk Cetin, Tugba Ataseven, Ilkay Kalkanli and Bulent Eren
Diagnostics 2025, 15(5), 605; https://doi.org/10.3390/diagnostics15050605 - 2 Mar 2025
Viewed by 568
Abstract
Objectives: Determination of the postmortem interval (PMI) remains a critical challenge in forensic science. Intervertebral discs, due to their structural resilience, hold promise as a reliable tissue for PMI estimation; however, studies focusing on their forensic applicability remain limited. This study aimed [...] Read more.
Objectives: Determination of the postmortem interval (PMI) remains a critical challenge in forensic science. Intervertebral discs, due to their structural resilience, hold promise as a reliable tissue for PMI estimation; however, studies focusing on their forensic applicability remain limited. This study aimed to evaluate progressive histopathological changes in intervertebral discs at specific postmortem intervals and assess their forensic applicability. Materials and Methods: A total of 48 rats were divided into six groups: control (0 h), 7-day, 15-day, 30-day, 60-day, and 90-day postmortem intervals. Intervertebral disc samples were stained with hematoxylin–eosin and trichrome, and histopathological parameters such as homogenization, eosinophilia, dissociation, nuclear alterations (pyknosis and karyolysis), and collagen fragmentation were analyzed. Results: Statistically significant changes were observed across postmortem intervals (p < 0.001). Homogenization progressed from mild changes at 7 days to prominent levels by 90 days. Eosinophilia and dissociation between the epithelium and connective tissue also increased significantly over time (p < 0.001). Collagen fragmentation, initially minimal, became severe at the 90-day interval. The observed changes demonstrated a clear, time-dependent progression strongly correlating with the PMI. Conclusions: Our findings suggest that histopathological changes in intervertebral discs follow a consistent and time-dependent pattern, making them a potential forensic marker for PMI estimation. This has important implications for forensic science, as it offers an alternative tissue type that is less susceptible to early decomposition compared to soft tissues. These results suggest that the intervertebral disc is a promising tissue for PMI estimation, offering a complementary approach to existing forensic methods. Full article
(This article belongs to the Special Issue Diagnostic Methods in Forensic Pathology, Third Edition)
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