Electrochemistry Technologies in Bioanalysis and Electrochemical Immunosensor

A special issue of Electrochem (ISSN 2673-3293).

Deadline for manuscript submissions: closed (28 December 2023) | Viewed by 2274

Special Issue Editor


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Guest Editor
Institute of Chemistry, Hebrew University of Jerusalem, Jerusalem 9190401, Israel
Interests: electrochemical immunosensors; nanobiotechnology; electro-organic transformation; electrochemical energy storage; electro-analytical catalysis

Special Issue Information

Dear Colleagues,

Electrochemistry technologies in bioanalysis represent the culmination of a comprehensive understanding of the benefits and challenges of applying electrochemical and electroanalytical-based techniques in analyzing biological samples. Proper detailing of the measurement of samples from the host with/without pre-sampling steps is required. This Special Issue is dedicated to covering the real insights for key experimental designs, measurements in different biological environments, mechanistic pathways, and theoretical aspects defining the principle of analysis, its bottlenecks, and its edge over different conventional prototypes. Discussion of various emerging topics of immunosensor fabrication, biocatalysis, bioadditives for better fabrication of electrodes, the interaction of biological samples with the transducer element, the essential selectivity and specificity for focusing on different proteins is emphasized. Furthermore, the role of electrochemistry technologies in the different potential areas of food and environmental analysis, disease biomarkers/metabolites, water pollution, microbial detection, etc., is also of interest. In this respect, different techniques are explored, such as voltammetric, impedimetric potentiometric, and conductometric-based signals. The use of separation science in conjugation with the electrochemical detection of bioanalysis provides an edge over classical methods and is being prompted by advancements in novel sensing materials and manufacturing approaches.

Dr. Mansi Gandhi
Guest Editor

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Keywords

  • electroanalytical
  • bioanalysis
  • electrochemical immunosensors
  • experimental design
  • transducer element
  • chemically modified electrodes
  • biomarker detection
  • separation science
  • fabrication
  • point-of-care devices

Published Papers (1 paper)

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Research

10 pages, 2376 KiB  
Article
Electrochemical Sensing of Amoxicillin Drug-Assisted Uropathogenic E. coli Bacteria Using Gold Nanostructures—A Preliminary Study
by Jayaprakash Sushmitha and Subramanian Nellaiappan
Electrochem 2023, 4(2), 291-300; https://doi.org/10.3390/electrochem4020020 - 13 Jun 2023
Viewed by 1800
Abstract
The present study focuses on the electrochemical sensing of amoxicillin (AMX, as a model antibiotic drug) and its interaction with Uropathogenic E. coli (UPEC) bacteria (as a model pathogen) under physiological conditions. The electrochemical sensor probe is formulated by nanostructured gold wires (AuNWs) [...] Read more.
The present study focuses on the electrochemical sensing of amoxicillin (AMX, as a model antibiotic drug) and its interaction with Uropathogenic E. coli (UPEC) bacteria (as a model pathogen) under physiological conditions. The electrochemical sensor probe is formulated by nanostructured gold wires (AuNWs) embedded in a carbon nanofiber–chitosan (CNF-CHIT) matrix. The synthesis of AuNWs is characterized by scanning electron microscopy (SEM), UV-Visible spectrophotometry, and X-ray photoelectron spectroscopy (XPS). The CNF-CHIT/AuNW-modified system is characterized by SEM and XPS. Initially, the CNF-CHIT/AuNW electrode was utilized for the sensing of AMX; later, in the antibiotic drug-assisted sensing of UPEC, i.e., in the presence of AMX, the interaction of UPEC was studied. The modified electrode showed appreciable sensitivity for AMX sensing; also, the interaction of AMX with UPEC is studied at two different conditions. One, at a fixed concentration of AMX (100 µM) and different concentrations of UPEC bacteria (0.6–1.2 × 106 CFU/mL), and another with incubation time (1 h–1 h 35 min) for bacterial reaction. The electrochemical antimicrobial resistance developed by UPEC, which is inherent in the sensing of AMX, is the key concept for the detection of pathogens. Full article
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