Strategies for Optimal Fermentation by Using Modern Tools and Methods

A special issue of Fermentation (ISSN 2311-5637). This special issue belongs to the section "Fermentation Process Design".

Deadline for manuscript submissions: 15 December 2024 | Viewed by 2200

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Institute of Microbiology Bulgarian Academy of Sciences, Sofia, Bulgaria
Interests: microalgae; lipids; cultivation; kinetics modeling; photobioreactors modeling; downstream; biodiesel; industrial-scale biodiesel production
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Special Issue Information

Dear Colleagues,

The optimization of fermentation processes to produce biomass, fuels, and chemicals has always faced challenges. The current state of the art highlights the complexity of achieving success in all areas of fermentation, emphasizing that success cannot be achieved without first applying methods that isolate and identify microorganisms from habitats or communities, or methods for improving the function of existing microorganisms. Notably, special attention will be given to the following: 1. manuscripts on the identification of microorganisms by target amplification methods and nucleic acid sequence analysis; 2. nanoparticles as a tool to enhance mass transfer processes and metabolic control during fermentation; 3. innovative culturing techniques to facilitate fermentation processes; 4. the development of subsystems techniques involving microbial kinetics, liquid flow distribution in the bioreactors, and heat and mass transfers. Additionally, manuscripts containing innovative models, response surface analysis, and artificial intelligence-based neural networks to facilitate and improve process design, optimization, control, and large-scale applications are welcome.

Prof. Dr. Alexander Dimitrov Kroumov
Guest Editor

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Keywords

  • fermentation process
  • metabolic control
  • process modeling
  • design
  • optimization

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Published Papers (4 papers)

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Research

16 pages, 3305 KiB  
Article
Innovative Approaches to Camembert Cheese: Optimizing Prebiotics and Coagulation Conditions for Enhanced Quality and Nutrition
by Adiba Benahmed Djilali, Mohammed Said Metahri, Lynda Lakabi, Hichem Tahraoui, Abdelouahab Benseddik, Colette Besombes and Karim Allaf
Fermentation 2024, 10(10), 524; https://doi.org/10.3390/fermentation10100524 (registering DOI) - 15 Oct 2024
Abstract
The objective of this study is to investigate how different factors, such as lactic acid bacteria, prebiotics (flaxseed powder, watercress seed powder, okra mucilage), and coagulation temperature influence the final quality of curd by conducting three optimization experiments and implementing a structured experimental [...] Read more.
The objective of this study is to investigate how different factors, such as lactic acid bacteria, prebiotics (flaxseed powder, watercress seed powder, okra mucilage), and coagulation temperature influence the final quality of curd by conducting three optimization experiments and implementing a structured experimental plan. In the first phase, milk coagulation was assessed at 45 °C with various combinations of lactic acid bacteria (probiotics) and prebiotics (powdered flaxseed and watercress). In the second investigation phase, the effects of lowered probiotic and prebiotic (powdered flaxseed and watercress) concentrations were examined at the coagulation temperature of 38 °C. We investigated the concentration of lactic acid bacteria at 3 mg/mL of milk and the effects of temperature and prebiotics (okra mucilage and flaxseed powder). We observed short milk clotting time (2 s) using the optimized mixture (0.18 mg of probiotics, 1.5 mg of flaxseed powder, and 1.147 mg of watercress powder) per 10 mL of milk. It contrasts with the classical coagulation way optimized at (5.9 and 9.5 s), which were generated at optimal temperatures of 45 and 45.7 °C, respectively. Our new mixture improves the fermentation process of camembert cheese at 38 °C. This cheese had a high flavonoid content, fewer lactic bacteria and molds, a homogeneous texture, and no outer crust, and exceptional sensory attributes such as a creamy and fluid paste. These attributes suggest its potential benefits as a dairy product for individuals with cardiovascular and gastrointestinal conditions. Full article
(This article belongs to the Special Issue Strategies for Optimal Fermentation by Using Modern Tools and Methods)
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18 pages, 1660 KiB  
Article
Microbial Biocapsules as Generally Recognized-As-Safe Fungal-Based Immobilized Cell Technology for Precision Sequential Fermentations of Grape Must
by Juan Carbonero-Pacheco, Florina Constanta-Mustafa, Raquel Muñoz-Castells, Juan Carlos Mauricio, Juan Moreno, Teresa García-Martínez and Jaime Moreno-García
Fermentation 2024, 10(10), 498; https://doi.org/10.3390/fermentation10100498 - 27 Sep 2024
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Abstract
This work focuses on the production of a white wine with a specific organoleptic profile by means of sequential fermentation using immobilized yeast in a system known as “microbial biocapsules”. Three fermentation conditions were created: sequential fermentation with immobilized yeast (SqFMB) employing a [...] Read more.
This work focuses on the production of a white wine with a specific organoleptic profile by means of sequential fermentation using immobilized yeast in a system known as “microbial biocapsules”. Three fermentation conditions were created: sequential fermentation with immobilized yeast (SqFMB) employing a matrix composed by Aspergillus oryzae (pellet-forming fungus recognized as GRAS), sequential fermentations with non-immobilized yeast cells (SqF), and a control of spontaneous fermentation (SpF). To carry out these fermentations, Pedro Ximénez grape must was used and two non-Saccharomyces yeast strains, Debaryomyces hansenii LR1 and Metschnikowia pulcherimma Primaflora, and the Saccharomyces cerevisiae X5 strain were used. The wines produced were subjected to microbiological and chemical analyses in which metabolites that positively influence the wine profile, such as 1,1-diethoxyethane and decanal, are only produced in the SqFMB condition, and others, like nonanal, were detected in higher concentrations than in SqF and SpF. Microbiological analyses show that less non-Saccharomyces yeasts were isolated in the SqFMB condition than in SqF, which indicates an efficiency in the inoculation and removal method proposed. These results conclude that microbial biocapsules seem to be a good yeast carrier for wine elaboration; however, modulation of some variables like yeast concentration inocula, the employment of preadaptation methods or the use of yeast species with higher fermentative power need to be tested to improve the novel methodology. Full article
(This article belongs to the Special Issue Strategies for Optimal Fermentation by Using Modern Tools and Methods)
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18 pages, 4825 KiB  
Article
Optimization of Bacterial Cellulose Production from Waste Figs by Komagataeibacter xylinus
by Merve Yilmaz and Yekta Goksungur
Fermentation 2024, 10(9), 466; https://doi.org/10.3390/fermentation10090466 - 9 Sep 2024
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Abstract
This study aimed to use waste figs as an alternative substrate for bacterial cellulose (BC) production by Komagataeibacter xylinus and optimize the identified process parameters to maximize the concentration of BC. Among the nutrients screened by Plackett–Burman (PB) design, yeast extract was found [...] Read more.
This study aimed to use waste figs as an alternative substrate for bacterial cellulose (BC) production by Komagataeibacter xylinus and optimize the identified process parameters to maximize the concentration of BC. Among the nutrients screened by Plackett–Burman (PB) design, yeast extract was found to be significant in BC production. Response surface methodology was used to investigate the effect of fermentation parameters on BC production. A maximum BC concentration of (8.45 g/L), which is among the highest BC concentrations reported so far, was achieved at the optimum levels of fermentation variables (initial pH 6.05, initial sugar concentration 62.75 g/L, temperature 30 °C). The utilization of response surface methodology (RSM) proved valuable in both optimizing and finding the interactions between process variables during BC production. Scanning electron microscope (SEM) analysis showed a dense structure of BC, characterized by ribbon-like nanofibrils with diameters ranging from 23 to 90 nm while the attenuated total reflection–Fourier transform infrared (ATR-FTIR) spectrum of BC confirmed that the material obtained was cellulose. The X-ray diffraction (XRD) analysis showed that the crystallinity of the BC samples was 70% for BC produced on waste fig medium and 61% for BC produced on Hestrin–Schramm (HS) medium. This is the first detailed study on the production of BC from waste figs, and the findings of this study demonstrated that waste figs can be used as an effective substrate for the production of BC. Full article
(This article belongs to the Special Issue Strategies for Optimal Fermentation by Using Modern Tools and Methods)
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18 pages, 2515 KiB  
Article
Thermal Treatment and Fermentation of Legume Flours with Leuconostoc citreum TR116 for the Development of Spreadable Meat Alternatives
by Aylin W. Sahin, Ophélie Gautheron and Sandra Galle
Fermentation 2024, 10(8), 412; https://doi.org/10.3390/fermentation10080412 - 9 Aug 2024
Viewed by 801
Abstract
The demand for meat alternatives in different forms is increasing due to consumers’ awareness of climate change and the health benefits of plant-based ingredients compared to animals. However, current alternatives on the market do not fulfil consumers’ acceptance for taste and texture. Hence, [...] Read more.
The demand for meat alternatives in different forms is increasing due to consumers’ awareness of climate change and the health benefits of plant-based ingredients compared to animals. However, current alternatives on the market do not fulfil consumers’ acceptance for taste and texture. Hence, different physical and biological processes, such as thermal treatment and fermentation, need to be investigated. This study reveals that the thermal treatment of legume flours (soy, pea and lentil) prior to single-strain fermentation with Leuconostoc citreum TR116 has a major impact on acidification, colour, texture and sensory properties due to the pregelatinisation of starch and denaturation of proteins. The thermal treatment of soy flour resulted in liquification, and it could not be used as a fermentation substrate. However, non-heat-treated soy flour was fermented for comparison. The highest total titratable acidity (TTA) was determined in fermented pea flour (PF) and fermented lentil flour (LF) after 48 h with 24.35 ± 0.29 mL 0.1 M NaOH/10 g and 24.98 ± 0.33 mL 0.1 M NaOH/10 g, respectively. Heat treatment prior to fermentation led to a reduction in TTA by 20 mL 0.1 M NaOH/10 g for both PF and LF. The loss of colour pigments during thermal treatment led to a lighter colour of the spreadable alternatives. Moreover, a harder texture (+13.76 N in LF; +15.13 N in PF) and a lower adhesiveness (−0.88 N in LF; −0.43 N in PF) were detected in spreadable meat alternatives that were treated with heat prior to fermentation. Cohesiveness was decreased by thermal treatment, and fermentation did not impact it. Fermentation without pre-heat treatment increased adhesiveness by 4.37 N in LF and by 2.36 N in PF—an attribute typical for spreadable meat. Descriptive sensory analysis showed that thermal treatment significantly decreased bitterness but increased crumbliness and reduced juiciness. On the other hand, fermentation without pre-heat treatment mainly influenced flavour by increasing fruitiness and decreasing beaniness, earthiness and off-flavours. In summary, thermal treatment prior to fermentation is powerful in reducing legume-typical off-flavours but is not suitable for the development of spreadable meat alternatives due to texture changes. However, this process can be very beneficial when producing sausage-like alternatives. Full article
(This article belongs to the Special Issue Strategies for Optimal Fermentation by Using Modern Tools and Methods)
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