Molecular Mechanism of Fish Immune Response to Pathogens

A special issue of Fishes (ISSN 2410-3888). This special issue belongs to the section "Fish Pathology and Parasitology".

Deadline for manuscript submissions: 17 October 2025 | Viewed by 907

Special Issue Editor


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Guest Editor
School of Wetlands, Yancheng Teachers University, Yancheng 224007, China
Interests: fish; genomics; immunity; transcriptome; toxicology
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Special Issue Information

Dear Colleagues,

This Special Issue aims to explore the molecular underpinnings of fish immune responses to pathogens, addressing critical gaps in the understanding of aquatic immunology and its applications in aquaculture and disease management. The scope encompasses studies on pathogen recognition mechanisms such as pattern recognition receptors including TLRs and NLRs, signaling pathways such as cytokine networks and NF-κB activation, and effector mechanisms including antimicrobial peptide production and phagocytosis that define fish immunity. By integrating comparative insights from plant and mammalian systems, such as stress adaptation pathways and transcriptomic regulation, the issue seeks to advance the knowledge of evolutionarily conserved and species-specific immune strategies in fish. The Special Issue situates itself within the broader literature by bridging gaps between fundamental immunological research and applied aquaculture challenges. It emphasizes cross-disciplinary parallels, such as the role of epigenetic modifications in immune memory and the application of omics technologies, including transcriptomics, proteomics, and single-cell omics, to map pathogen–host interactions. Contributions will focus on mechanistic discoveries like immune cell differentiation and apoptosis regulation, as well as translational innovations such as vaccine development and immunostimulant design. By synthesizing molecular insights with ecological and aquaculture priorities, this issue aims to catalyze targeted research on sustainable disease control in fisheries. We invite researchers to submit their work to this Special Issue on “Molecular Mechanism of Fish Immune Response to Pathogens” in Fishes, fostering a dynamic frontier in comparative immunology and biotechnology.

Prof. Dr. Qiuning Liu
Guest Editor

Manuscript Submission Information

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Keywords

  • fish
  • immune response
  • pathogens
  • omics
  • molecular mechanism

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Published Papers (2 papers)

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Research

17 pages, 3797 KB  
Article
Transcriptomic and Metabolomic Analyses Provide Insights into Cryptocaryon irritans Resistance in Golden Pompano (Trachinotus ovatus)
by Bo Liu, Bao-Suo Liu, Jing-Wen Yang, Hua-Yang Guo, Nan Zhang, Teng-Fei Zhu, Lin Xian, Ke-Cheng Zhu and Dian-Chang Zhang
Fishes 2025, 10(9), 426; https://doi.org/10.3390/fishes10090426 - 1 Sep 2025
Viewed by 279
Abstract
Golden pompano (Trachinotus ovatus) is an economically important fish species along China’s southern coast. However, infections by Cryptocaryon irritans severely constrain the healthy and sustainable development of the aquaculture industry. To investigate the genetic basis of resistance to this parasite in [...] Read more.
Golden pompano (Trachinotus ovatus) is an economically important fish species along China’s southern coast. However, infections by Cryptocaryon irritans severely constrain the healthy and sustainable development of the aquaculture industry. To investigate the genetic basis of resistance to this parasite in golden pompano, this study employed transcriptomic and metabolomic analyses to compare differences between susceptible (ES) and resistant (RS) groups following C. irritans challenge. Transcriptome analysis identified 2031 differentially expressed genes (DEGs) between EST and RST groups, comprising 1004 up-regulated and 1027 down-regulated genes. Gene Ontology (GO) functional annotation and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment revealed that these DEGs were primarily enriched in lipid metabolism and amino acid metabolism pathways. Untargeted metabolomics detected 461 significantly differentially abundant metabolites (295 up-regulated, 166 down-regulated), confirming pronounced metabolic differences between ES and RS groups, particularly in lipid and amino acid metabolism. Further, KEGG enrichment highlighted steroid hormone biosynthesis, α-linolenic acid metabolism, and arachidonic acid metabolism as the most significantly altered pathways upon infection. This integrated transcriptomic and metabolomic study reveals substantial differences in gene expression and metabolite profiles between susceptible and resistant golden pompano in response to C. irritans. These changes predominantly involve lipid metabolism and amino acid metabolism, suggesting that these processes are critical in determining host resistance/susceptibility. Full article
(This article belongs to the Special Issue Molecular Mechanism of Fish Immune Response to Pathogens)
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18 pages, 2761 KB  
Article
Transcriptomic Profiling of Misgurnus anguillicaudatus Reveals the Anti-Inflammatory Action of Lonicera japonica Extract in Response to Lipopolysaccharide Challenge
by Yue Zhao, Chen Wang and Qiuning Liu
Fishes 2025, 10(7), 333; https://doi.org/10.3390/fishes10070333 - 7 Jul 2025
Viewed by 412
Abstract
Honeysuckle, derived from the dried flower buds or blossoms of Lonicera japonica Thunb, is a traditional Chinese medicine known for its properties in eliminating heat and toxins, reducing inflammation, and alleviating swelling. In this study, we investigated the potential therapeutic and preventive benefits [...] Read more.
Honeysuckle, derived from the dried flower buds or blossoms of Lonicera japonica Thunb, is a traditional Chinese medicine known for its properties in eliminating heat and toxins, reducing inflammation, and alleviating swelling. In this study, we investigated the potential therapeutic and preventive benefits of L. japonica extract on inflammatory diseases induced by lipopolysaccharide (LPS) using Misgurnus anguillicaudatus as a model organism. The fish were fed a diet supplemented with L. japonica extract, followed by LPS injection to induce inflammation. We then analyzed the transcriptional profile to identify differentially expressed genes (DEGs). A total of 6611 DEGs were identified through comprehensive analysis, including Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses. Our results revealed significant enrichment of DEGs in pathways associated with proteasome function, immune system regulation, and infectious disease response. These findings suggest a strong correlation between L. japonica and immune defense mechanisms, providing valuable insights into the potential anti-inflammatory effects of this plant, particularly in the context of LPS-induced inflammation. This study highlights the potential use of L. japonica in treating inflammatory diseases and underscores its role in immune regulation. Full article
(This article belongs to the Special Issue Molecular Mechanism of Fish Immune Response to Pathogens)
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