Applications of Plant Biotechnology and Molecular Biology in Fruit Crops

A special issue of Horticulturae (ISSN 2311-7524). This special issue belongs to the section "Developmental Physiology, Biochemistry, and Molecular Biology".

Deadline for manuscript submissions: closed (20 June 2023) | Viewed by 7278

Special Issue Editors


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Guest Editor
Department of Orchard Plant Genetics and Biotechnology, Institute of Horticulture, Lithuanian Research Centre for Agriculture and Forestry, 54333 Babtai, Lithuania
Interests: horticultural plant breeding; biotechnology; physiology; stress resistance; cryopreservation
Special Issues, Collections and Topics in MDPI journals

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Guest Editor
Institute of Horticulture, Lithuanian Research Centre for Agriculture and Forestry, LT-54333 Kaunas, Lithuania
Interests: breeding; fruit; plant physiology; genetics; plant biotechnology; plant genetics; plant biology; plant breeding; agriculture

E-Mail Website1 Website2
Guest Editor
Institute of Horticulture, Lithuanian Research Centre for Agriculture and Forestry, LT-54333 Kaunas, Lithuania
Interests: plant breeding; biotechnology; molecular genetics; phytopathology

Special Issue Information

Dear Colleagues,

Unlike agricultural plants, fruit plants have multiple features, such as asexual reproduction, a long juvenile phase, and the requirements of controlled conditions, grafting, post-harvest treatment. The genomes of horticultural plants are highly diverse and complex, often with a high degree of heterozygosity and a high ploidy due to their long and complex history of evolution and domestication. Due to all of these concerns, it is difficult to investigate fruit plant features in model plants or by using conventional tools. Recent technologies, such as quantitative trait loci (QTL) mapping, whole-genome resequencing and genotyping by sequencing, phenomics, transcriptomics and proteomics, and genome editing utilizing CRISPR-Cas technology, allow plant breeders to develop/transfer important genomic regions to elite cultivars with great precision. New approaches help to address important issues. For example, endophytic bacteria are shown to have several beneficial effects on their host plant, including growth-promoting activity, a reduction in oxidative injury levels, and the modulation of plant metabolism and phytohormone signaling that leads to an adaptation to environmental abiotic or biotic stresses. It is also important to look for natural antagonists in various plant pathogens. Stress responses are associated with an accumulation of plant secondary metabolites important for human health, such as anthocyanins, ellagic acid and others. The manipulation of the underlying regulatory mechanisms during fruit ripening suggests ways to enhance the desired pigments in fruits by biotechnological interventions. Fruit crop plants can be a platform for pharmaceutical protein production by plant molecular farming. For many fruit crops, in vitro propagation, plant nutrition, genetic and epigenetic stability, light regulation, and the preservation of genetic resources or cryopreservation remain very important.

The proposed Special Issue, entitled "Applications of Plant Biotechnology and Molecular Biology in Fruit Crops", aims to present the results of recent research studies, methods, technologies, and innovative practices in fruit crops. We look forward to receiving your manuscripts and sharing the achievements of this rapidly evolving field.

Dr. Rytis Rugienius
Prof. Dr. Vidmantas Stanys
Dr. Birutė Frercks
Guest Editors

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Keywords

  • genome mapping
  • abiotic and biotic stress resistance
  • secondary metabolites
  • endophytes
  • productivity

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Published Papers (3 papers)

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Research

18 pages, 9782 KiB  
Article
Plant Growth Promoting and Colonization of Endophytic Streptomyces albus CINv1 against Strawberry Anthracnose
by Waraporn Pupakdeepan, Natthida Termsung, On-Uma Ruangwong and Kaewalin Kunasakdakul
Horticulturae 2023, 9(7), 766; https://doi.org/10.3390/horticulturae9070766 - 4 Jul 2023
Cited by 2 | Viewed by 2765
Abstract
Strawberry anthracnose is a serious disease, and fungicides are currently widely used by farmers. Thus, biological control is a good alternative. This study aims to identify the species of endophytic Streptomyces CINv1 that was previously isolated from Cinnamomum verum J. Presl. and to [...] Read more.
Strawberry anthracnose is a serious disease, and fungicides are currently widely used by farmers. Thus, biological control is a good alternative. This study aims to identify the species of endophytic Streptomyces CINv1 that was previously isolated from Cinnamomum verum J. Presl. and to evaluate its properties as a biocontrol agent, plant growth promoter, and plant colonizing endophyte. This strain was identified by analysis of its 16S rRNA gene sequences, and the result shows 100% similarity to Streptomyces albus CINv1. The CINv1 strain displayed high resistance (81.83%) against Colletotrichum sp. isolate CA0110, as tested by the dual culture technique. Additionally, inhibited pathogen growth on IMA-2 agar was observed under a compound microscope. The results demonstrated swelling, bulbousness, and cytoplasmic aggregation of abnormal hyphal, which were confirmed by SEM as well. Furthermore, the functional media used to evaluate plant growth-promoting properties, including nitrogen fixation, phosphate solubilization, and siderophore production, yielded positive results. Analyses of plant hormones by HPLC found their ability to produce indole-3-acetic acid (IAA). Thus, a biological control trial in greenhouse conditions was conducted by spraying a spore suspension of the strain onto strawberry seedlings once a week, which showed a significant reduction in disease severity. After the seventh spraying, the assessment of the number of leaves and canopy height of the seedling showed significant promotion. In addition, the CINv1 strain established a mutualistic interaction with the plant cells through colonization inter-and intracellularly in strawberry roots, leaves, and petioles. Moreover, using LC-MS/MS to analyze the secondary metabolites of this strain, various groups of compounds were found that could potentially benefit pharmaceutical and agricultural uses. Full article
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15 pages, 3080 KiB  
Article
Genome-Wide Identification and Expression Profile Analysis of the WUSCHEL-Related Homeobox (WOX) Genes in Woodland Strawberry (Fragaria vesca)
by Xu Yang, Xinyong Zhao, Yanan Miao, Dongxue Wang, Zhihong Zhang and Yuexue Liu
Horticulturae 2022, 8(11), 1043; https://doi.org/10.3390/horticulturae8111043 - 7 Nov 2022
Cited by 4 | Viewed by 2015
Abstract
The WUSCHEL-related homeobox (WOX) is a unique transcription factor family belonging to plants. Its members play important roles in regulation of plant stem cell division and differentiation or dynamic balance of the organ development. In this study, 16 Fragaria vesca [...] Read more.
The WUSCHEL-related homeobox (WOX) is a unique transcription factor family belonging to plants. Its members play important roles in regulation of plant stem cell division and differentiation or dynamic balance of the organ development. In this study, 16 Fragaria vesca WOX members (FvWOXs) were identified in the woodland strawberry (Fragaria vesca) genome. According to the phylogenetic analysis, FvWOX proteins can be classified into three clades: the ancient clade, the WUS clade and the intermediate clade. The gene structure and protein motif analysis showed that FvWOXs are highly conserved to each other in their respective clades. Chromosome location analysis revealed that FvWOXs are unevenly distributed in the Fragaria vesca chromosomes. Expression analysis showed that FvWOX genes are widely expressed in various tissues of Fragaria vesca. Moreover, when treated with 6-benzylaminopurine (6-BA) or gibberellin (GA3), expression level changes of the genes highly expressed in crowns were detected at 48 h after treatment. The subcellular localization of FvWUSb was determined in tobacco leaves, indicating that the protein is located in the nucleus. This study enlarged our recognition about the WUSCHEL-related homeobox genes in Fragaria vesca, and laid a foundation for further researches about this gene family in strawberry. Full article
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19 pages, 9910 KiB  
Article
Characterization and Antagonistic Effect of Culturable Apple-Phyllosphere Endophytic Bacteria from the Cold Plateau in Yunnan, China
by Xinlong Pan, Pengbo He, Cong Zhou, Haichun Cun, Pengfei He, Shahzad Munir, Yixin Wu, Ayesha Ahmed, Suhail Asad, Jun Ma, Yongcui Ma, Yanming Zhang, Keqiang Cao, Baohua Kong and Yueqiu He
Horticulturae 2022, 8(11), 991; https://doi.org/10.3390/horticulturae8110991 - 26 Oct 2022
Cited by 2 | Viewed by 1894
Abstract
The endophytic bacteria in apple leaves from apple-producing areas of Yunnan, China were isolated and identified on the basis of bacterial colony morphology and nucleotide sequences of 16S rRNA and rpoB genes. The endophytic bacterial isolates with nitrogen, phosphorus, and potassium utilization abilities [...] Read more.
The endophytic bacteria in apple leaves from apple-producing areas of Yunnan, China were isolated and identified on the basis of bacterial colony morphology and nucleotide sequences of 16S rRNA and rpoB genes. The endophytic bacterial isolates with nitrogen, phosphorus, and potassium utilization abilities were screened by culturing on functional media. A total of 5709 isolates of culturable endophytic bacteria (CEB) were isolated from 30 apple leaf samples collected from different regions. A total of 39 CEB representative isolates were identified as Bacillus velezensis, B. subtilis, B. licheniformis, B. safensis, B. pumilus, and Priestia megaterium. Among them, B. velezensis and B. subtilis were the main CEB, accounting for 55.00% and 34.37%, respectively, which exhibited potential inhibition on not only the main apple disease pathogens of Alternaria alternata, Valsa mali, Fusarium oxysporum, and Rosellinia necatrix, but also some important and uncontrollable phytopathogens, including F. oxysporum f. sp. cubense that causes banana Fusarium wilt, and Phytophthora nicotianae that causes tobacco black shank. Among these isolated endophytic bacteria species, a total of 10 strains, including b3, b4, b16, b17, b20, and b23 of B. subtilis, b7, b24, and b28 of B. licheniformis, and b38 of B. velezensis, can fix nitrogen; 8 strains, including b7 and b28 of B. licheniformis, b5, b10, and b23 of B. subtilis, b8 of B. safensis, and b6 of Priestia megaterium, could dissolve inorganic phosphorus; 11 strains, including b9, b12, b14, b30, b34, and b43 of B. velezensis, b6 of Priestia megaterium, and b17, b18, b20, and b26 of B. subtilis, could degrade organic phosphorus; and 5 strains, including b4, b5, and b26 of B. subtilis, and b7 and b28 of B. licheniformis could dissolve potassium. These strains are valuable resources of endophytic bacteria that have adapted to the ecological environment of the Cold Plateau apple-production area and could be used as plant disease biocontrol agents and biofertilizers of crops. The culturable phyllosphere endophytes in apple leaves relate to geographic locations, apple varieties, and environment, providing the basis to explain the mechanisms underlying the establishment of apple endophyte diversity and may help to devise apple disease management strategies. Full article
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