Dear Colleagues,

This Special Issue is the second volume of our previous Special Issue on “Mass Spectrometric Proteomics 2.0”. Proteomics is a still-growing field of molecular biology whose the goal is the systematic identification and quantification of the entire set of proteins (the proteome) expressed at a given time in a biological system (organism, tissue, cell, or biological fluid). Assuming that the variations observed in the proteomes of a system at different times, in response to a specific stimulus, would highlight differences between them, most proteomic (in parallel with metabolomics and genomics) efforts to date have been mainly directed toward biomarker research for a variety of disorders. As proteomics and genomics are complementary techniques, it is questionable what the former adds to the latter. Indeed, the variety of proteins that may be produced both as a result of alternative splicing at the RNA level and after translation (via processes such as phosphorylation, glycosylation, and proteolytic cleavage) makes proteomics more suitable than genomics for a comprehensive understanding of the biochemical processes that govern life. Understanding how proteins function and interact with one another is another goal of proteomics that makes this approach even more intriguing. Because of their ability to handle the complexity of the events mentioned above, mass spectrometry (MS)-based methods have become the primary technology to identify proteins that may be separated by one- and two-dimensional gel electrophoresis (1- and 2-DE) and/or via liquid chromatographic techniques (1- and 2D-LC). Currently, proteomics relies mainly on MS, and the numerous applications thus far described have contributed heavily to providing new insights into the roles played by some proteins in human disorders.

The aim of this Special Issue is to attract contributions on all aspects of MS-based proteomics, with special emphasis on recent/novel technologies that, by pushing the boundaries of MS capabilities, are able to address biological problems that have not yet been resolved.

Prof. Dr. Paolo Iadarola
Guest Editor

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• proteome
• mass spectrometry
• biological system
• genome
• protein forms
• biological phenotype
• expression, localization, interaction and domain structure of proteomics