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Biofabrication for Tissue Engineering Applications 2.0
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Biofabrication for Tissue Engineering Applications 2.0

A special issue of International Journal of Molecular Sciences (ISSN 1422-0067). This special issue belongs to the section "Materials Science".

Deadline for manuscript submissions: closed (31 July 2023) | Viewed by 4600

Special Issue Editor

Dr. Emmanuel Stratakis

E-Mail Website
Guest Editor
Foundation for Research and Technology-Hellas (F.O.R.T.H.), Institute of Electronic Structure and Laser (I.E.S.L.), 70013 Heraklion, Greece
Interests: biosensors; bioelectronics; biomaterials; tissue engineering; biosurfaces; biointerfaces
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

The concept of regenerating tissues with properties and functions that mimic natural tissues has attracted significant attention in recent years since it provides potential solutions for many disease treatments and demanding healthcare problems. To fully realize the potential of this approach, it is crucial to have a rational biomaterial design and subsequent fabrication to create novel scaffolds, material systems and devices suitable for tissue engineering, repair, and regeneration. As a consequence of the intense research activity in the field, a variety of 3D and 4D biofabrication approaches has been developed, including soft lithography and self-assembly, as well as subtractive (top-down), additive (bottom-up) and hybrid manufacturing. Further research advances for this topic include the design of new and smart biomaterials, the fabrication of implantable multifunctional scaffolds and devices for disease monitoring, diagnostics and treatment, as well as the manufacturing of artificial tissues and organs.

This Special Issue, entitled “Biofabrication for Tissue Engineering”, welcomes original research and review articles in this rapidly growing field that are related to the development of biomaterial scaffolds, biomedical devices and organ-on-a-chip systems for tissue engineering applications. It will focus on all aspects of biofabrication, including the design, manufacturing, functionalization, characterization, evaluation of novel scaffolds, biomedical devices and systems for tissue engineering and regeneration, aiming at disease diagnoses and treatments.

Dr. Emmanuel Stratakis
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. International Journal of Molecular Sciences is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. There is an Article Processing Charge (APC) for publication in this open access journal. For details about the APC please see here. Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • biofabrication
  • biomaterials processing
  • tissue engineering systems
  • advanced bioimaging

Published Papers (3 papers)

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Research

18 pages, 5253 KiB  
Article
Optimization of Enzymatic and Chemical Decellularization of Native Porcine Heart Valves for the Generation of Decellularized Xenografts
by Monireh Saeid Nia, Lena Maria Floder, Jette Anika Seiler, Thomas Puehler, Nina Sophie Pommert, Rouven Berndt, David Meier, Stephanie L. Sellers, Janarthanan Sathananthan, Xiling Zhang, Mario Hasler, Stanislav N. Gorb, Gregor Warnecke and Georg Lutter
Int. J. Mol. Sci. 2024, 25(7), 4026; https://doi.org/10.3390/ijms25074026 - 04 Apr 2024
Viewed by 554
Abstract
One of the most important medical interventions for individuals with heart valvular disease is heart valve replacement, which is not without substantial challenges, particularly for pediatric patients. Due to their biological properties and biocompatibility, natural tissue-originated scaffolds derived from human or animal sources [...] Read more.
One of the most important medical interventions for individuals with heart valvular disease is heart valve replacement, which is not without substantial challenges, particularly for pediatric patients. Due to their biological properties and biocompatibility, natural tissue-originated scaffolds derived from human or animal sources are one type of scaffold that is widely used in tissue engineering. However, they are known for their high potential for immunogenicity. Being free of cells and genetic material, decellularized xenografts, consequently, have low immunogenicity and, thus, are expected to be tolerated by the recipient’s immune system. The scaffold ultrastructure and ECM composition can be affected by cell removal agents. Therefore, applying an appropriate method that preserves intact the structure of the ECM plays a critical role in the final result. So far, there has not been an effective decellularization technique that preserves the integrity of the heart valve’s ultrastructure while securing the least amount of genetic material left. This study demonstrates a new protocol with untraceable cells and residual DNA, thereby maximally reducing any chance of immunogenicity. The mechanical and biochemical properties of the ECM resemble those of native heart valves. Results from this study strongly indicate that different critical factors, such as ionic detergent omission, the substitution of Triton X-100 with Tergitol, and using a lower concentration of trypsin and a higher concentration of DNase and RNase, play a significant role in maintaining intact the ultrastructure and function of the ECM. Full article
(This article belongs to the Special Issue Biofabrication for Tissue Engineering Applications 2.0)
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13 pages, 140162 KiB  
Article
3D-Cultured Adipose-Derived Stem Cell Spheres Using Calcium-Alginate Scaffolds for Osteoarthritis Treatment in a Mono-Iodoacetate-Induced Rat Model
by Yu-Ying Lin, Che-Yung Kuan, Chia-Tien Chang, Ming-Hsi Chuang, Wan-Sin Syu, Kai-Ling Zhang, Chia-Hsin Lee, Po-Cheng Lin, Guo-Chung Dong and Feng-Huei Lin
Int. J. Mol. Sci. 2023, 24(8), 7062; https://doi.org/10.3390/ijms24087062 - 11 Apr 2023
Cited by 2 | Viewed by 1873
Abstract
Osteoarthritis (OA) is a degenerative disease that causes pain, cartilage deformation, and joint inflammation. Mesenchymal stem cells (MSCs) are potential therapeutic agents for OA treatment. However, the 2D culture of MSCs could potentially affect their characteristics and functionality. In this study, calcium-alginate (Ca-Ag) [...] Read more.
Osteoarthritis (OA) is a degenerative disease that causes pain, cartilage deformation, and joint inflammation. Mesenchymal stem cells (MSCs) are potential therapeutic agents for OA treatment. However, the 2D culture of MSCs could potentially affect their characteristics and functionality. In this study, calcium-alginate (Ca-Ag) scaffolds were prepared for human adipose-derived stem cell (hADSC) proliferation with a homemade functionally closed process bioreactor system; the feasibility of cultured hADSC spheres in heterologous stem cell therapy for OA treatment was then evaluated. hADSC spheres were collected from Ca-Ag scaffolds by removing calcium ions via ethylenediaminetetraacetic acid (EDTA) chelation. In this study, 2D-cultured individual hADSCs or hADSC spheres were evaluated for treatment efficacy in a monosodium iodoacetate (MIA)-induced OA rat model. The results of gait analysis and histological sectioning showed that hADSC spheres were more effective at relieving arthritis degeneration. The results of serological and blood element analyses of hADSC-treated rats indicated that the hADSC spheres were a safe treatment in vivo. This study demonstrates that hADSC spheres are a promising treatment for OA and can be applied to other stem cell therapies or regenerative medical treatments. Full article
(This article belongs to the Special Issue Biofabrication for Tissue Engineering Applications 2.0)
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14 pages, 4978 KiB  
Article
Electrospun Hyaluronan Nanofiber Membrane Immobilizing Aromatic Doxorubicin as Therapeutic and Regenerative Biomaterial
by Xiaowen Han, Mingda Zhao, Ruiling Xu, Yaping Zou, Yuxiang Wang, Jie Liang, Qing Jiang, Yong Sun, Yujiang Fan and Xingdong Zhang
Int. J. Mol. Sci. 2023, 24(8), 7023; https://doi.org/10.3390/ijms24087023 - 10 Apr 2023
Cited by 4 | Viewed by 1587
Abstract
Lesioned tissue requires synchronous control of disease and regeneration progression after surgery. It is necessary to develop therapeutic and regenerative scaffolds. Here, hyaluronic acid (HA) was esterified with benzyl groups to prepare hyaluronic acid derivative (HA-Bn) nanofibers via electrospinning. Electrospun membranes with average [...] Read more.
Lesioned tissue requires synchronous control of disease and regeneration progression after surgery. It is necessary to develop therapeutic and regenerative scaffolds. Here, hyaluronic acid (HA) was esterified with benzyl groups to prepare hyaluronic acid derivative (HA-Bn) nanofibers via electrospinning. Electrospun membranes with average fiber diameters of 407.64 ± 124.8 nm (H400), 642.3 ± 228.76 nm (H600), and 841.09 ± 236.86 nm (H800) were obtained by adjusting the spinning parameters. These fibrous membranes had good biocompatibility, among which the H400 group could promote the proliferation and spread of L929 cells. Using the postoperative treatment of malignant skin melanoma as an example, the anticancer drug doxorubicin (DOX) was encapsulated in nanofibers via hybrid electrospinning. The UV spectroscopy of DOX-loaded nanofibers (HA-DOX) revealed that DOX was successfully encapsulated, and there was a π–π interaction between aromatic DOX and HA-Bn. The drug release profile confirmed the sustained release of about 90%, achieved within 7 days. In vitro cell experiments proved that the HA-DOX nanofiber had a considerable inhibitory effect on B16F10 cells. Therefore, the HA-Bn electrospun membrane could facilitate the potential regeneration of injured skin tissues and be incorporated with drugs to achieve therapeutic effects, offering a powerful approach to developing therapeutic and regenerative biomaterial. Full article
(This article belongs to the Special Issue Biofabrication for Tissue Engineering Applications 2.0)
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