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Recent Advances on Bioreceptors and Nanomaterial-Based Biosensors

A special issue of International Journal of Molecular Sciences (ISSN 1422-0067). This special issue belongs to the section "Biochemistry".

Deadline for manuscript submissions: closed (20 April 2025) | Viewed by 4153

Special Issue Editor


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Guest Editor
The Chair of Medical Biotechnology, Faculty of Chemistry, Warsaw University of Technology, Noakowskiego 3, 00-664 Warsaw, Poland
Interests: nucleic acids and antibodies; biosensor sensing layers; molecular recognition; the construction and analysis of receptor layers of affinity biosensors (nucleic acids, including aptamers and antibodies); the construction of ready-made sensor solutions, substrates manufactured using printed electronics technology
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Special Issue Information

Dear Colleagues,

The need for selective detection and determination of low concentrations of certain analytes in the shortest possible time of analysis has prompted continuous efforts aimed at the design and construction of new microanalytical devices. Of much interest in this area are chemical sensors, along with their specific type of biological-origin components in the analyte-identifying layer, called biosensors.

Depending on the composition of the above mentioned receptor layer, it is possible to detect and determine almost any compound of interest, from simple ions to organic compounds, peptides, proteins nucleic acids or even whole cells. Because of the available versatile organic synthesis methods, which provide us with the possibility to conjugate chosen biological molecules or adjust their sequence or structure, it is possible, on the one hand, to increase their specificity towards chosen analyte, and on the other, to increase their resistance to environmental conditions, making them significantly more useful in real-life applications. This is further supported by developed new nanomaterials (nanoparticles, graphene, quantum dots, etc.). They can be used as markers, substrates or carriers for chosen receptors, which allows for an almost unlimited elaboration of bio(sensors) with analytical parameters specifically adjusted to the given application. Such features make these devices very attractive for modern analytical methods.

This Special Issue of IJMS is focused on recent molecular advances in biosensors. We welcome submissions of research articles, reviews, etc.

Dr. Robert Ziółkowski
Guest Editor

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Keywords

  • biosensor
  • nanomaterials
  • microfluidic devices
  • mems
  • detection techniques
  • aptamers
  • antibodies

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Published Papers (3 papers)

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Research

12 pages, 2643 KiB  
Article
A Novel Methylene Blue Indicator-Based Aptasensor for Rapid Detection of Pseudomonas aeruginosa
by Somayeh Maghsoomi, Julia Walochnik, Martin Brandl and Mai-Lan Pham
Int. J. Mol. Sci. 2024, 25(21), 11682; https://doi.org/10.3390/ijms252111682 - 30 Oct 2024
Viewed by 1244
Abstract
Pseudomonas aeruginosa is a significant opportunistic pathogen highly prevalent in the environment, requiring early detection methods to prevent infections in vulnerable individuals. The most specific aptamer for P. aeruginosa, F23, has been used for the development of various assays and sensors for [...] Read more.
Pseudomonas aeruginosa is a significant opportunistic pathogen highly prevalent in the environment, requiring early detection methods to prevent infections in vulnerable individuals. The most specific aptamer for P. aeruginosa, F23, has been used for the development of various assays and sensors for early diagnosis and monitoring. In this study, a novel F23-based electrochemical aptasensor was designed using disposal gold screen-printed electrodes (Au-SPEs) with high reproducibility. Methylene blue (MB) was used as an exogenous indicator, which significantly amplified the electrochemical signal and improved the sensitivity of detection. The aptasensor explored a limit of detection (LOD) of 8 CFU·mL−1 and high selectivity for P. aeruginosa over other interfering bacteria. Furthermore, it showed potential to detect P. aeruginosa in tap water samples, offering a point-of-care tool for rapidly controlling the growth of this bacterium in various applications. Full article
(This article belongs to the Special Issue Recent Advances on Bioreceptors and Nanomaterial-Based Biosensors)
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12 pages, 3899 KiB  
Article
Hybrid Polystyrene–Plasmonic Systems as High Binding Density Biosensing Platforms
by Charles M. Darr, Juiena Hasan, Cherian Joseph Mathai, Keshab Gangopadhyay, Shubhra Gangopadhyay and Sangho Bok
Int. J. Mol. Sci. 2024, 25(16), 8603; https://doi.org/10.3390/ijms25168603 - 7 Aug 2024
Viewed by 992
Abstract
Sensitive, accurate, and early detection of biomarkers is essential for prompt response to medical decisions for saving lives. Some infectious diseases are deadly even in small quantities and require early detection for patients and public health. The scarcity of these biomarkers necessitates signal [...] Read more.
Sensitive, accurate, and early detection of biomarkers is essential for prompt response to medical decisions for saving lives. Some infectious diseases are deadly even in small quantities and require early detection for patients and public health. The scarcity of these biomarkers necessitates signal amplification before diagnosis. Recently, we demonstrated single-molecule-level detection of tuberculosis biomarker, lipoarabinomannan, from patient urine using silver plasmonic gratings with thin plasma-activated alumina. While powerful, biomarker binding density was limited by the surface density of plasma-activated carbonyl groups, that degraded quickly, resulting in immediate use requirement after plasma activation. Therefore, development of stable high density binding surfaces such as high binding polystyrene is essential to improving shelf-life, reducing binding protocol complexity, and expanding to a wider range of applications. However, any layers topping the plasmonic grating must be ultra-thin (<10 nm) for the plasmonic enhancement of adjacent signals. Furthermore, fabricating thin polystyrene layers over alumina is nontrivial because of poor adhesion between polystyrene and alumina. Herein, we present the development of a stable, ultra-thin polystyrene layer on the gratings, which demonstrated 63.8 times brighter fluorescence compared to commercial polystyrene wellplates. Spike protein was examined for COVID-19 demonstrating the single-molecule counting capability of the hybrid polystyrene-plasmonic gratings. Full article
(This article belongs to the Special Issue Recent Advances on Bioreceptors and Nanomaterial-Based Biosensors)
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16 pages, 2617 KiB  
Article
Aptamer and Electrochemical Aptasensor towards Selenate Ions (SeO42−)
by Anna Szymczyk, Martyna Popiołek, Dominika Baran, Marcin Olszewski, Robert Ziółkowski and Elżbieta Malinowska
Int. J. Mol. Sci. 2024, 25(12), 6660; https://doi.org/10.3390/ijms25126660 - 17 Jun 2024
Cited by 1 | Viewed by 1171
Abstract
Selenium is an essential inorganic compound in human and animal nutrition, involved in the proper functioning of the body. As a micronutrient, it actively contributes to the regulation of various metabolic activities, i.e., thyroid hormone, and protection against oxidative stress. However, Se exhibits [...] Read more.
Selenium is an essential inorganic compound in human and animal nutrition, involved in the proper functioning of the body. As a micronutrient, it actively contributes to the regulation of various metabolic activities, i.e., thyroid hormone, and protection against oxidative stress. However, Se exhibits a narrow concentration window between having a positive effect and exerting a toxic effect. In higher doses, it negatively affects living organisms and causes DNA damage through the formation of free radicals. Increased reactivity of Se anions can also disrupt the integrity and function of DNA-repairing proteins. As the permissible concentration of Se in drinking water is 10 µg/L, it is vital to develop sensitive and robust methods of Se detection in aqueous samples. In this study, for the first time, we proposed a selective aptamer for selenate ion detection, chosen following the SELEX process, and its application in the construction of an electrochemical aptasensor towards SeO42− ions. Measurement conditions such as the used redox marker and pH value of the measurement solution were chosen. The proposed aptasensor is characterized by good selectivity and an LOD of 1 nM. Conditions for biosensor regeneration and storage were also investigated in this research. Full article
(This article belongs to the Special Issue Recent Advances on Bioreceptors and Nanomaterial-Based Biosensors)
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