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IJMS
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Molecular Spectroscopic Approaches in Biochemistry and Microbiology
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Molecular Spectroscopic Approaches in Biochemistry and Microbiology

A special issue of International Journal of Molecular Sciences (ISSN 1422-0067). This special issue belongs to the section "Molecular Microbiology".

Deadline for manuscript submissions: closed (31 December 2020) | Viewed by 2736

Special Issue Editor

Prof. Dr. Alexander A. Kamnev

E-Mail Website
Guest Editor
Laboratory of Biochemistry, Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, Saratov 410049, Russia
Interests: development and applications of molecular spectroscopy techniques in microbiogy; FTIR and Raman biospectroscopy; mossbauer spectroscopy in life sciences
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Modern spectroscopic techniques have proven to be indispensable in research related to life sciences, where the objects ranging from biocomplexes to supramolecular structures and further to cells and tissues are commonly highly complicated. Spectroscopic approaches allow their structural and compositional features to be investigated at the molecular level, which is often unattainable by other means. Moreover, many spectroscopic techniques are non-destructive, which is of paramount importance for biological samples. This Special Issue of IJMS will include a collection of expert-level experimental and review-type papers where the main results were obtained using a wide range of modern spectroscopic techniques applied to biochemical and microbiological problems.

Prof. Dr. Alexander A. Kamnev
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. International Journal of Molecular Sciences is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. There is an Article Processing Charge (APC) for publication in this open access journal. For details about the APC please see here. Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • biochemistry
  • microbiology
  • molecular spectroscopy
  • microbial cells
  • cellular components
  • metabolic processes
  • microbial ecology

Published Papers (1 paper)

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Research

17 pages, 4487 KiB  
Article
Detecting Bacterial Cell Viability in Few µL Solutions from Impedance Measurements on Silicon-Based Biochips
by Vinayak J. Bhat, Sahitya V. Vegesna, Mahdi Kiani, Xianyue Zhao, Daniel Blaschke, Nan Du, Manja Vogel, Sindy Kluge, Johannes Raff, Uwe Hübner, Ilona Skorupa, Lars Rebohle and Heidemarie Schmidt
Int. J. Mol. Sci. 2021, 22(7), 3541; https://doi.org/10.3390/ijms22073541 - 29 Mar 2021
Cited by 2 | Viewed by 2335
Abstract
Using two different types of impedance biochips (PS5 and BS5) with ring top electrodes, a distinct change of measured impedance has been detected after adding 1–5 µL (with dead or live Gram-positive Lysinibacillus sphaericus JG-A12 cells to 20 µL DI water inside the [...] Read more.
Using two different types of impedance biochips (PS5 and BS5) with ring top electrodes, a distinct change of measured impedance has been detected after adding 1–5 µL (with dead or live Gram-positive Lysinibacillus sphaericus JG-A12 cells to 20 µL DI water inside the ring top electrode. We relate observed change of measured impedance to change of membrane potential of L. sphaericus JG-A12 cells. In contrast to impedance measurements, optical density (OD) measurements cannot be used to distinguish between dead and live cells. Dead L. sphaericus JG-A12 cells have been obtained by adding 0.02 mg/mL of the antibiotics tetracycline and 0.1 mg/mL chloramphenicol to a batch with OD0.5 and by incubation for 24 h, 30 °C, 120 rpm in the dark. For impedance measurements, we have used batches with a cell density of 25.5 × 108 cells/mL (OD8.5) and 270.0 × 108 cells/mL (OD90.0). The impedance biochip PS5 can be used to detect the more resistive and less capacitive live L. sphaericus JG-A12 cells. Also, the impedance biochip BS5 can be used to detect the less resistive and more capacitive dead L. sphaericus JG-A12 cells. An outlook on the application of the impedance biochips for high-throughput drug screening, e.g., against multi-drug-resistant Gram-positive bacteria, is given. Full article
(This article belongs to the Special Issue Molecular Spectroscopic Approaches in Biochemistry and Microbiology)
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