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The Research of Neutrophil 2.0

A special issue of International Journal of Molecular Sciences (ISSN 1422-0067). This special issue belongs to the section "Molecular Immunology".

Deadline for manuscript submissions: closed (31 December 2022) | Viewed by 3140

Special Issue Editor


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Guest Editor
Department of Laboratory Diagnostics and Clinical Immunology of Developmental Age, Medical University of Warsaw, Warsaw, Poland
Interests: immunology; inflammation; tumor development; autoimmunity; neutrophil extracellular traps
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Neutrophils are the most abundant, short-lived, and terminally differentiated white blood cells. They constitute the first line of defense against foreign invaders using major effector mechanisms: phagocytosis, degranulation, and NETs formation. Traditionally neutrophils were considered as terminally differentiated, homogenous cell populations of the innate immune response, however, different studies started to highlight the subpopulation heterogeneity and pivotal role in immunity,  inflammation and cancer. All scientists involved in the field of neutrophil biology are welcome to contribute to the understanding of the role of neutrophil in physiology and pathology, providing clues for better management and newer therapeutic opportunities for neutrophil-centric disorders. Both original and review papers are welcome.

Prof. Dr. Urszula Demkow
Guest Editor

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Keywords

  • neutrophil
  • innate immune response
  • immunity
  • inflammation
  • neutrophil-centric disorder

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Published Papers (1 paper)

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Research

17 pages, 3858 KiB  
Article
Neutrophil Extracellular Traps Mediate Bovine Endometrial Epithelial Cell Pyroptosis in Dairy Cows with Endometritis
by Wenxiang Shen, Xiaoyu Ma, Dan Shao, Xiaohu Wu, Shengyi Wang, Juanshan Zheng, Yanan Lv, Xuezhi Ding, Baohua Ma and Zuoting Yan
Int. J. Mol. Sci. 2022, 23(22), 14013; https://doi.org/10.3390/ijms232214013 - 13 Nov 2022
Cited by 7 | Viewed by 2456
Abstract
Neutrophils are involved in the development of endometritis, but it remains unknown how neutrophils induce inflammation and tissue damage. Neutrophil extracellular traps (NETs) clear invading pathogens during infection but induce pyroptosis, leading to inflammation and tissue damage. Thus, our objective was to investigate [...] Read more.
Neutrophils are involved in the development of endometritis, but it remains unknown how neutrophils induce inflammation and tissue damage. Neutrophil extracellular traps (NETs) clear invading pathogens during infection but induce pyroptosis, leading to inflammation and tissue damage. Thus, our objective was to investigate whether NETs participate in bovine endometrial epithelial cell (BEEC) pyroptosis during endometritis. To confirm this, NETs and caspase-1/4; apoptosis-associated speck-like protein containing a caspase-recruitment domain(ASC); nod-like receptor protein-3 (NLRP3); and gasdermin D N-terminal (GSDMD-N), TNF-a, IL-1β, IL-6, and IL-18 in endometrial tissue were detected. Pathological section and vaginal discharge smears were performed to visually determine endometritis in the uterus. BEECs were stimulated with NETs to induce pyroptosis, which was treated with DNase I against pyroptosis. Caspase-1/4, ASC, NLRP3, GSDMD-N, TNF-a, IL-1β, IL-6, and IL-18 in BEECs were analyzed in endometrial tissue. The results showed that NET formation, as well as pyroptosis-related proteins and proinflammatory, cytokines were elevated in the endometrial tissue of cows with endometritis. Pathological sections and vaginal discharge smears showed increased neutrophils and plasma cells in the uterus, as well as tissue congestion. In BEECs, NETs increased the level of pyroptosis-related proteins and proinflammatory cytokines and were diminished by DNase I. In summary NETs participate BEEC pyroptosis during endometritis in dairy cows. Full article
(This article belongs to the Special Issue The Research of Neutrophil 2.0)
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