State of the Art: Three-Dimensional Printing Materials and Regenerative Medicine

A special issue of Journal of Functional Biomaterials (ISSN 2079-4983). This special issue belongs to the section "Biomaterials for Tissue Engineering and Regenerative Medicine".

Deadline for manuscript submissions: 31 December 2024 | Viewed by 735

Special Issue Editor


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Guest Editor
Henry M. Rowan College of Engineering, Department of Chemical Engineering and Biomedical Engineering, Rowan University, Glassboro, NJ, USA
Interests: biomaterials; biofabrication; bioassembly; 3D printing; hydrogels

Special Issue Information

Dear Colleagues,

This Special Issue of the Journal of Functional Biomaterials delves into the intersection of advanced manufacturing technologies and regenerative medicine, aiming to explore the latest developments, challenges and applications of 3D printing materials in the context of tissue engineering and regenerative medicine. By showcasing cutting-edge research and innovative methodologies, the Special Issue seeks to provide insights into the design, fabrication and characterization of biomaterials for 3D printing, as well as their applications for tissue regeneration and repair. Additionally, this collection of articles will contribute to the existing literature by offering a comprehensive overview of the current trends and future directions in the field. By situating itself within the broader landscape of biomaterials science and regenerative medicine, the Special Issue aims to foster an interdisciplinary dialogue and stimulate further advancements in this rapidly evolving field.

Dr. Andrea Jennifer Vernengo
Guest Editor

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Keywords

  • biofabrication
  • bioassembly
  • tissue models
  • organ models
  • bioprinting
  • spheroids
  • bioinks
  • biomaterials

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Published Papers (1 paper)

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Research

17 pages, 6452 KiB  
Article
3D-Printable Gelatin Methacrylate-Xanthan Gum Hydrogel Bioink Enabling Human Induced Pluripotent Stem Cell Differentiation into Cardiomyocytes
by Virginia Deidda, Isabel Ventisette, Marianna Langione, Lucrezia Giammarino, Josè Manuel Pioner, Caterina Credi and Federico Carpi
J. Funct. Biomater. 2024, 15(10), 297; https://doi.org/10.3390/jfb15100297 - 5 Oct 2024
Viewed by 565
Abstract
We describe the development of a bioink to bioprint human induced pluripotent stem cells (hiPSCs) for possible cardiac tissue engineering using a gelatin methacrylate (GelMA)-based hydrogel. While previous studies have shown that GelMA at a low concentration (5% w/v) allows [...] Read more.
We describe the development of a bioink to bioprint human induced pluripotent stem cells (hiPSCs) for possible cardiac tissue engineering using a gelatin methacrylate (GelMA)-based hydrogel. While previous studies have shown that GelMA at a low concentration (5% w/v) allows for the growth of diverse cells, its 3D printability has been found to be limited by its low viscosity. To overcome that drawback, making the hydrogel both compatible with hiPSCs and 3D-printable, we developed an extrudable GelMA-based bioink by adding xanthan gum (XG). The GelMA-XG composite hydrogel had an elastic modulus (~9 kPa) comparable to that of cardiac tissue, and enabled 3D printing with high values of printing accuracy (83%) and printability (0.98). Tests with hiPSCs showed the hydrogel’s ability to promote their proliferation within both 2D and 3D cell cultures. The tests also showed that hiPSCs inside hemispheres of the hydrogel were able to differentiate into cardiomyocytes, capable of spontaneous contractions (average frequency of ~0.5 Hz and amplitude of ~2%). Furthermore, bioprinting tests proved the possibility of fabricating 3D constructs of the hiPSC-laden hydrogel, with well-defined line widths (~800 μm). Full article
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