Advanced Metabolic Profiling via Gas Chromatography–Mass Spectrometry and Liquid Chromatography–Mass Spectrometry

A special issue of Metabolites (ISSN 2218-1989). This special issue belongs to the section "Metabolomic Profiling Technology".

Deadline for manuscript submissions: 15 January 2025 | Viewed by 960

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Laboratory of Bromatology and Hydrology, Faculty of Pharmacy, University of Porto, Rua Jorge Viterbo Ferreira 228, 4050-313 Porto, Portugal
Interests: metabolomics; environmental sciences; toxicology; biomarkers
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Special Issue Information

Dear Colleagues,

In the intricate world of metabolic research, the techniques of Gas Chromatography–Mass Spectrometry (GC–MS) and Liquid Chromatography–Mass Spectrometry (LC–MS) have proven to be more than analytical tools; they are pivotal catalysts in our quest to decode the complex narrative of biological systems. This Special Issue, "Advanced Metabolic Profiling via Gas Chromatography–Mass Spectrometry and Liquid Chromatography–Mass Spectrometry", seeks to spotlight the transformative impact these methodologies have on our ability to advance our understanding of metabolic pathways and their multifaceted implications.

GC–MS and LC–MS have revolutionized metabolic profiling, offering unparalleled specificity, sensitivity, and versatility in detecting and quantifying metabolites. These techniques stand at the forefront of metabolic research, enabling scientists to unravel the complexities of the metabolome with remarkable precision. The ability of GC–MS to separate and analyze volatile and semi-volatile compounds, coupled with the versatility of LC–MS to manage a broad spectrum of polar and nonpolar compounds, has significantly broadened the horizon of metabolic studies, facilitating comprehensive analyses that were once deemed challenging.

The relevance of these techniques extends beyond the bounds of academic curiosity; they are instrumental in addressing real-world challenges across various sectors. In healthcare, they are transforming disease diagnosis and drug development, offering insights into biomarker discovery and therapeutic interventions. In environmental science, they serve as crucial tools for monitoring pollutants and deciphering ecological dynamics. In the food industry, they ensure safety and quality, upholding the highest standards of consumer health and product integrity.

This Special Issue is a call upon researchers and innovators who are exploring the potentials of GC–MS and LC–MS to push the boundaries of metabolic research. We invite you to contribute original research articles or comprehensive review papers that not only highlight methodological advancements but also demonstrate how these techniques enhance our understanding of biological, environmental, and societal aspects. By sharing your cutting-edge research, you are contributing to a critical discourse that has the power to reshape industries, inform policymaking, and ultimately improve lives. Our aim is to compile a repository of the most innovative and impactful research in this field. Your insights and findings will be invaluable, serving as a cornerstone for future exploration and discovery.

Dr. Ana Margarida Araújo
Guest Editor

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Keywords

  • chromatographic methods
  • mass spectrometry techniques
  • metabolic profiling
  • methodological advancements
  • biomarker discovery
  • environmental monitoring
  • food safety and quality
  • clinical diagnosis
  • drug development

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Published Papers (1 paper)

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12 pages, 1323 KiB  
Article
Characterization and Metabolism of Drug Products Containing the Cocaine-Like New Psychoactive Substances Indatraline and Troparil
by Sascha K. Manier, Paula Mumber, Josef Zapp, Niels Eckstein and Markus R. Meyer
Metabolites 2024, 14(6), 342; https://doi.org/10.3390/metabo14060342 - 18 Jun 2024
Viewed by 651
Abstract
With a rising demand of cocaine over the last years, it is likely that unregulated new psychoactive substances with similar effects such as indatraline ((1R,3S)-3-(3,4-dichlorophenyl)-N-methyl-2,3-dihydro-1H-inden-1-amine) and troparil (Methyl (1R,2S,3S,5 [...] Read more.
With a rising demand of cocaine over the last years, it is likely that unregulated new psychoactive substances with similar effects such as indatraline ((1R,3S)-3-(3,4-dichlorophenyl)-N-methyl-2,3-dihydro-1H-inden-1-amine) and troparil (Methyl (1R,2S,3S,5S)-8-methyl-3-phenyl-8-azabicyclo[3.2.1]octane-2-carboxylate) become popular as well. Both substances share a similar pharmacological profile as cocaine, while their potency is higher, and their duration of action is longer. This study investigated their metabolic fate in rat urine and incubations using pooled human liver S9 fraction (pHLS9). Indatraline formed two phase I and four phase II metabolites, with aromatic hydroxylation and glucuronidation being the main metabolic steps. All metabolites were detected in rat urine, while the parent compound was not detectable. Although low in abundance, indatraline metabolites were well identifiable due to their specific isotopic patterns caused by chlorine. Troparil formed four phase I and three phase II metabolites, with demethylation being the main metabolic step. Hydroxylation of the tropane ring, the phenyl ring, and combinations of these steps, as well as glucuronidation, were found. Phase I metabolites were detectable in rat urine and pHLS9, while phase II metabolites were only detectable in rat urine. Full article
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