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Advances in Plant Specialized Metabolites: How to Take Full Advantages...
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Advances in Plant Specialized Metabolites: How to Take Full Advantages of the Properties of Specialized Metabolites?

A special issue of Metabolites (ISSN 2218-1989). This special issue belongs to the section "Plant Metabolism".

Deadline for manuscript submissions: closed (15 November 2021) | Viewed by 15752

Special Issue Editors

Dr. David Gagneul

E-Mail Website
Guest Editor
UMR-t INRAE 1158 BioEcoAgro, University of Lille, Villeneuve d’Ascq, F-59655 Lille, France
Interests: plant physiology; molecular plant biology; phytochemistry; metabolomics; specialized metabolites; terpenes and terpenoids; biosynthesic pathways
Special Issues, Collections and Topics in MDPI journals
Dr. Romain Larbat

E-Mail Website
Guest Editor
Université de Lorraine – INRAE Laboratoire Agronomie et Environnement (LAE), UMR 1121. F54000 Nancy, France
Interests: plant specialized metabolism; phytochemistry; plant ecophysiology; crop protection; bioactive compounds
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Plant specialized metabolites refer to a huge set of molecules with highly diverse structures and biological roles. They are crucial for plants living in a challenging environment.. In addition, these bioactive compounds are of special interest for human uses in different field such as medicine, cosmetic, food and agriculture. To take advantage of these extraordinary properties, efforts are needed to completely decipher their metabolism and their regulation, to elucidate their roles in planta and their biological properties and to develop biotechnological tools to ensure supply for existing or new applications.

This special issue is dedicated to all disciplinary fields related to plant specialized metabolites. In particular, we welcome research papers and reviews that deal with:

  • Biosynthesis of specialized metabolites
  • Regulation of specialized metabolism
  • Roles of specialized metabolism in plants
  • Evolution of specialized metabolism
  • Biological properties of plant specialized metabolites
  • Biotechnological strategies for production of plant specialized metabolites
  • Uses of specialized metabolites in agriculture, medicine, cosmetic…

Dr. David Gagneul
Dr. Romain Larbat
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Metabolites is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • Plant specialized metabolites
  • Biochemistry
  • Biotechnology
  • Biological properties
  • Biocontrol
  • Abiotic and biotic stresses
  • Metabolic engineering

Published Papers (5 papers)

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Research

17 pages, 3143 KiB  
Article
Knockout of Arabidopsis thaliana VEP1, Encoding a PRISE (Progesterone 5β-Reductase/Iridoid Synthase-Like Enzyme), Leads to Metabolic Changes in Response to Exogenous Methyl Vinyl Ketone (MVK)
by Jan Klein, Mona Ernst, Alexander Christmann, Marina Tropper, Tim Leykauf, Wolfgang Kreis and Jennifer Munkert
Metabolites 2022, 12(1), 11; https://doi.org/10.3390/metabo12010011 - 23 Dec 2021
Cited by 6 | Viewed by 2568
Abstract
Small or specialized natural products (SNAPs) produced by plants vary greatly in structure and function, leading to selective advantages during evolution. With a limited number of genes available, a high promiscuity of the enzymes involved allows the generation of a broad range of [...] Read more.
Small or specialized natural products (SNAPs) produced by plants vary greatly in structure and function, leading to selective advantages during evolution. With a limited number of genes available, a high promiscuity of the enzymes involved allows the generation of a broad range of SNAPs in complex metabolic networks. Comparative metabolic studies may help to understand why—or why not—certain SNAPs are produced in plants. Here, we used the wound-induced, vein patterning regulating VEP1 (AtStR1, At4g24220) and its paralogue gene on locus At5g58750 (AtStR2) from Arabidopsis to study this issue. The enzymes encoded by VEP1-like genes were clustered under the term PRISEs (progesterone 5β-reductase/iridoid synthase-like enzymes) as it was previously demonstrated that they are involved in cardenolide and/or iridoid biosynthesis in other plants. In order to further understand the general role of PRISEs and to detect additional more “accidental” roles we herein characterized A. thaliana steroid reductase 1 (AtStR1) and compared it to A. thaliana steroid reductase 2 (AtStR2). We used A. thaliana Col-0 wildtype plants as well as VEP1 knockout mutants and VEP1 knockout mutants overexpressing either AtStR1 or AtStR2 to investigate the effects on vein patterning and on the stress response after treatment with methyl vinyl ketone (MVK). Our results added evidence to the assumption that AtStR1 and AtStR2, as well as PRISEs in general, play specific roles in stress and defense situations and may be responsible for sudden metabolic shifts. Full article
(This article belongs to the Special Issue Advances in Plant Specialized Metabolites: How to Take Full Advantages of the Properties of Specialized Metabolites?)
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19 pages, 3177 KiB  
Article
Unravelling the Puzzle of Anthranoid Metabolism in Living Plant Cells Using Spectral Imaging Coupled to Mass Spectrometry
by Quentin Chevalier, Jean-Baptiste Gallé, Nicolas Wasser, Valérie Mazan, Claire Villette, Jérôme Mutterer, Maria Mercedes Elustondo, Nicolas Girard, Mourad Elhabiri, Hubert Schaller, Andréa Hemmerlin and Catherine Vonthron-Sénécheau
Metabolites 2021, 11(9), 571; https://doi.org/10.3390/metabo11090571 - 25 Aug 2021
Cited by 2 | Viewed by 3266
Abstract
Vismione H (VH) is a fluorescent prenylated anthranoid produced by plants from the Hypericaceae family, with antiprotozoal activities against malaria and leishmaniosis. Little is known about its biosynthesis and metabolism in plants or its mode of action against parasites. When VH is isolated [...] Read more.
Vismione H (VH) is a fluorescent prenylated anthranoid produced by plants from the Hypericaceae family, with antiprotozoal activities against malaria and leishmaniosis. Little is known about its biosynthesis and metabolism in plants or its mode of action against parasites. When VH is isolated from Psorospermum glaberrimum, it is rapidly converted into madagascine anthrone and anthraquinone, which are characterized by markedly different fluorescent properties. To locate the fluorescence of VH in living plant cells and discriminate it from that of the other metabolites, an original strategy combining spectral imaging (SImaging), confocal microscopy, and non-targeted metabolomics using mass spectrometry, was developed. Besides VH, structurally related molecules including madagascine (Mad), emodin (Emo), quinizarin (Qui), as well as lapachol (Lap) and fraxetin (Fra) were analyzed. This strategy readily allowed a spatiotemporal characterization and discrimination of spectral fingerprints from anthranoid-derived metabolites and related complexes with cations and proteins. In addition, our study validates the ability of plant cells to metabolize VH into madagascine anthrone, anthraquinones and unexpected metabolites. These results pave the way for new hypotheses on anthranoid metabolism in plants. Full article
(This article belongs to the Special Issue Advances in Plant Specialized Metabolites: How to Take Full Advantages of the Properties of Specialized Metabolites?)
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18 pages, 2159 KiB  
Article
Metabolomic Characterization of Commercial, Old, and Red-Fleshed Apple Varieties
by Adriana Teresa Ceci, Michele Bassi, Walter Guerra, Michael Oberhuber, Peter Robatscher, Fulvio Mattivi and Pietro Franceschi
Metabolites 2021, 11(6), 378; https://doi.org/10.3390/metabo11060378 - 11 Jun 2021
Cited by 13 | Viewed by 4092
Abstract
In this study, a metabolomic investigation was presented to correlate single polyphenolic compounds in apple pulp with quality characteristics such as antioxidant activity and content of phenolic compounds and anthocyanins in apple skin. Since the concentration of these compounds is influenced by environmental [...] Read more.
In this study, a metabolomic investigation was presented to correlate single polyphenolic compounds in apple pulp with quality characteristics such as antioxidant activity and content of phenolic compounds and anthocyanins in apple skin. Since the concentration of these compounds is influenced by environmental factors, the twenty-two apple cultivars originate from the same site. The polyphenolic compounds were analyzed by ultra-high-performance liquid chromatography coupled with triple quadrupole mass spectrometry (UHPLC-QqQ-MS/MS). The antioxidant activity, phenolic content, and anthocyanins were evaluated on the sunny and the shady sides of apple skin by spectrometric assays. In old apple varieties, the measured parameters were higher than in the commercial and red-fleshed varieties. By contrast, the profile of flavan-3-ols and anthocyanins was variable amongst commercial and red-fleshed varieties. The partial least square (PLS) method was applied to investigate the association between the skin proprieties and the metabolic profile of the pulp. The highest coefficients of determination in prediction (Q2) were obtained for compounds quantified in old cultivars. These results provided information to define the old apple varieties as a reliable group based on the pathway of the antioxidant compounds and anthocyanins content. Our results show the possibility to find cultivars with promising health features based on their content of polyphenols suitable for commercialization or breeding. Full article
(This article belongs to the Special Issue Advances in Plant Specialized Metabolites: How to Take Full Advantages of the Properties of Specialized Metabolites?)
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11 pages, 1591 KiB  
Article
Leafy Stems of Phagnalon saxatile subsp. saxatile from Algeriaas a Source of Chlorogenic Acids and Flavonoids with Antioxidant Activity: Characterization and Quantification Using UPLC-DAD-ESI-MSn
by Farah Haddouchi, Tarik Mohammed Chaouche, Riadh Ksouri and Romain Larbat
Metabolites 2021, 11(5), 280; https://doi.org/10.3390/metabo11050280 - 29 Apr 2021
Cited by 5 | Viewed by 2151
Abstract
Phagnalon saxatile subsp. saxatile is a wild species widespread in Algeria which is utilized for medicinal purposes as analgesic and anticholesterolemic. However, information is still scarce regarding its phytochemical content. The objective of this study was to identify and quantify the phenolic compounds [...] Read more.
Phagnalon saxatile subsp. saxatile is a wild species widespread in Algeria which is utilized for medicinal purposes as analgesic and anticholesterolemic. However, information is still scarce regarding its phytochemical content. The objective of this study was to identify and quantify the phenolic compounds from different extracts of its leafy stems. For this purpose, the effects of four extracting solvents were investigated on the content of phenolic compounds and the antioxidant activity of this plant. The extracts prepared with polar solvents (methanol and water) contained higher amounts of phenolic compounds and showed better antioxidant activity than the extracts with apolar solvents (hexane, dichloromethane). The methanolic extract, richest in total phenolic and total flavonoid, had significant antioxidant activity as regarded by DPPH° scavenging capacity (IC50 of 5.5 µg/mL), ABTS+° scavenging capacity (IC50 of 63.8 µg/mL) and inhibition of oxidation of linoleic acid (IC50 of 22.7 µg/mL), when compared to synthetic antioxidants. Chlorogenic acids and several flavonoids were identified and quantified by UPLC-DAD-MSn. The di-O-caffeoylquinic acids isomers were the most concentrated phenolics (25.4 mg/g DW) in the methanolic extract. Full article
(This article belongs to the Special Issue Advances in Plant Specialized Metabolites: How to Take Full Advantages of the Properties of Specialized Metabolites?)
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11 pages, 1978 KiB  
Article
The Interaction of the Flavonoid Fisetin with Human Glutathione Transferase A1-1
by Mohammed Hamed Alqarni, Ahmed Ibrahim Foudah, Magdy Mohamed Muharram and Nikolaos E. Labrou
Metabolites 2021, 11(3), 190; https://doi.org/10.3390/metabo11030190 - 23 Mar 2021
Cited by 7 | Viewed by 2406
Abstract
Glutathione transferases (GSTs) are a family of Phase II detoxification enzymes that are involved in the development of the multidrug resistance (MDR) mechanism in cancer cells and therefore affect the clinical outcome of cancer chemotherapy. The discovery of nontoxic natural compounds as inhibitors [...] Read more.
Glutathione transferases (GSTs) are a family of Phase II detoxification enzymes that are involved in the development of the multidrug resistance (MDR) mechanism in cancer cells and therefore affect the clinical outcome of cancer chemotherapy. The discovery of nontoxic natural compounds as inhibitors for GSTs is a promising approach for chemosensitizing and reversing MDR. Fisetin (7,3′,4′-flavon-3-ol) is a plant flavonol present in many plants and fruits. In the present work, the interaction of fisetin with human glutathione transferase A1-1 (hGSTA1-1) was investigated. Kinetic analysis revealed that fisetin is a reversible inhibitor for hGSTA1-1 with IC50 1.2 ± 0.1 μΜ. It functions as a mixed-type inhibitor toward glutathione (GSH) and as a noncompetitive inhibitor toward the electrophile substrate 1-chloro-2,4-dinitrobenzene (CDNB). In silico molecular modeling and docking predicted that fisetin binds at a distinct location, in the solvent channel of the enzyme, and occupies the entrance of the substrate-binding sites. Treatment of proliferating human epithelial colorectal adenocarcinoma cells (CaCo-2) with fisetin causes a reduction in the expression of hGSTA1-1 at the mRNA and protein levels. In addition, fisetin inhibits GST activity in CaCo-2 cell crude extract with an IC50 (2.5 ± 0.1 μΜ), comparable to that measured using purified recombinant hGSTA1-1. These actions of fisetin can provide a synergistic role toward the suppression and chemosensitization of cancer cells. The results of the present study provide insights into the development of safe and effective GST-targeted cancer chemosensitizers. Full article
(This article belongs to the Special Issue Advances in Plant Specialized Metabolites: How to Take Full Advantages of the Properties of Specialized Metabolites?)
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