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Micromachines
Special Issues
Microfluidics-Based Point-of-Care Diagnostics
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Microfluidics-Based Point-of-Care Diagnostics

A special issue of Micromachines (ISSN 2072-666X). This special issue belongs to the section "B：Biology and Biomedicine".

Deadline for manuscript submissions: closed (31 March 2020) | Viewed by 2701

Special Issue Editor

Prof. Dong-Ku Kang

E-Mail Website
Guest Editor
Department of Chemistry, Incheon National University119 Academy-ro, Yeonsu-gu, Incheon, Korea
Interests: microfluidics; lab-on-a-chip; droplet microfluidics; point-of-care diagnostics; liquid biopsy; biomarkers; infectious bacteria; circulating tumor DNA/RNA; single cell analysis; digital PCR

Special Issue Information

Dear Colleagues,

Microfluidics has been increaingly used as a platform for technology in various research purposes across different scientific fields, because of its advantages. One of the important advantages of microfluidics is that it allows for the precise manipulation of a lower volume of samples, such as dilution, mixing, chemical reaction, and partitioning, within microfabricated channels. Eventually, microfludic technologies allow for the use and analysis of lower volumes of samles for rapid and sensitive detection. Accordingly, it has become a key platform technology for point-of-care (POC) diagnostics, because of its various advantages, including feasibility and portability. POC diagnostics have the potential to improve health care in various ways, ranging from enabling the earlier detection of disease and easier monitoring, to reaching under-served and remote populations. This is why POC diagnostics and microfluidics have merged rapidly, and have had a great impact on the rapid development of POC diagnostic technology. Accordingly, this Special Issue seeks recent developments of microfluidics with research papers, short communications, and review articles that focus on the novel methodological developments and applications of microfluidics devices for POC diagnostics.

Prof. Dong-Ku Kang
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Micromachines is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2600 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • Microfluidics
  • Lab-on-a-Chip
  • Droplet Microfluidics
  • Point-of-Care Diagnostics
  • Liquid Biopsy
  • Biomarkers
  • Single Cell Analysis
  • Digital PCR
  • Circulating Tumor DNA/RNA
  • Circulating Tumor Cells

Published Papers (1 paper)

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Research

10 pages, 3065 KiB  
Article
A Nut-and-Bolt Microfluidic Mixing System for the Rapid Labeling of Immune Cells with Antibodies
by Jakir Hossain Imran and Jung Kyung Kim
Micromachines 2020, 11(3), 280; https://doi.org/10.3390/mi11030280 - 9 Mar 2020
Cited by 5 | Viewed by 2422
Abstract
A nut-and-bolt microfluidic system was previously developed for a point-of-care (POC) human immunodeficiency virus (HIV) test and was able to acquire images of CD4 (cluster of differentiation 4) + T-lymphocytes in a sample drop of blood followed by image analysis. However, as the [...] Read more.
A nut-and-bolt microfluidic system was previously developed for a point-of-care (POC) human immunodeficiency virus (HIV) test and was able to acquire images of CD4 (cluster of differentiation 4) + T-lymphocytes in a sample drop of blood followed by image analysis. However, as the system was not fully integrated with a sample reaction module, the mixing of the sample with the antibody reagent was carried out manually. To achieve a rapid reaction with a reduced amount of costly reagent in a POC diagnostic system, an efficient sample mixing function must be implemented. Here, we propose a novel method to drastically accelerate the process of sample mixing and increase the reaction rate in the nut-and-bolt microfluidic system, where the sample is mixed with the reagent in a reaction chamber formed by connecting a nut with a bolt-like sample cartridge. The mixing is facilitated by rotating the sample cartridge bidirectionally using a DC motor, which agitates the sample in a chaotic manner. A microbead complex formed by the avidin–biotin interaction was used as a model reaction system to examine the feasibility of our mixing module. We found that the reaction time for the avidin–biotin binding by mixing was 7.5 times shorter than in the incubation method, achieving a reaction efficiency of over 95%. The performance of our mixing system was further demonstrated by measuring the concentration of CD4 cells labeled with a fluorescent antibody in the blood sample. The antigen–antibody reaction mixing was faster by a factor of 20, reaching a reaction efficiency comparable to the conventional incubation method. Full article
(This article belongs to the Special Issue Microfluidics-Based Point-of-Care Diagnostics)
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