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Microorganisms
Special Issues
Phage-Bacteria Interplay: Phage Biology and Phage Therapy
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Phage-Bacteria Interplay: Phage Biology and Phage Therapy

A special issue of Microorganisms (ISSN 2076-2607). This special issue belongs to the section "Virology".

Deadline for manuscript submissions: 20 August 2025 | Viewed by 5953

Special Issue Editors

Dr. Hesham Abdelbary

E-Mail Website
Guest Editor
Department of Surgery, Division of Orthopaedics, The Ottawa Hospital, Ottawa, ON, Canada
Interests: phage; periprosthetic infections
Special Issues, Collections and Topics in MDPI journals
Dr. Mariam Taha

E-Mail Website
Guest Editor
Ottawa Hospital Research Institute, Ottawa, ON, Canada
Interests: phage; biofilm

Special Issue Information

Dear Colleagues,

Bacteriophages (phages) are naturally occurring viruses that specifically infect and replicate within bacterial cells. Phages play a crucial role in shaping microbial communities and influencing bacterial evolution. Over the past few years, there has been a surge in exploring the therapeutic potential of phages against antibiotic-resistant bacteria.

This Special Issue entitled “Phage–Bacteria Interplay: Phage Biology and Phage Therapy” aims to present recent research related to phages with special focus on, but not limited to, the following areas: phage genomics, phage diversity, phage therapy, phage–host interactions, phage-mediated horizontal gene transfer, diverse mechanisms of phage infection, microbiome, replication and interaction with bacterial hosts. This Special Issue is devoted to publishing papers on studies of the biology, therapy and application of phages. Reviews, original research and communications will be welcome.

Dr. Hesham Abdelbary
Dr. Mariam Taha
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Microorganisms is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • microbiome
  • phage therapy
  • phage resistance
  • phage biology
  • phage engineering

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Further information on MDPI's Special Issue policies can be found here.

Published Papers (3 papers)

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Research

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26 pages, 9037 KiB  
Article
Isolation, Characterization, and Genomic Analysis of Bacteriophages Against Pseudomonas aeruginosa Clinical Isolates from Early and Chronic Cystic Fibrosis Patients for Potential Phage Therapy
by Hanzada T. Nour El-Din, Maryam Kettal, José C. Granados Maciel, Greg Beaudoin, Umut Oktay, Sabahudin Hrapovic, Subash Sad, Jonathan J. Dennis, Danielle L. Peters and Wangxue Chen
Microorganisms 2025, 13(3), 511; https://doi.org/10.3390/microorganisms13030511 - 26 Feb 2025
Viewed by 1362
Abstract
Pseudomonas aeruginosa is associated with both community and hospital-acquired infections. It colonizes the lungs of cystic fibrosis (CF) patients, establishing an ecological niche where it adapts and evolves from early to chronic stages, resulting in deteriorating lung function and frequent exacerbations. With antibiotics [...] Read more.
Pseudomonas aeruginosa is associated with both community and hospital-acquired infections. It colonizes the lungs of cystic fibrosis (CF) patients, establishing an ecological niche where it adapts and evolves from early to chronic stages, resulting in deteriorating lung function and frequent exacerbations. With antibiotics resistance on the rise, there is a pressing need for alternative personalized treatments (such as bacteriophage therapy) to combat P. aeruginosa infections. In this study, we aimed to isolate and characterize phages targeting both early and chronic P. aeruginosa isolates and evaluate their potential for phage therapy. Four highly virulent phages belonging to myoviral, podviral, and siphoviral morphotypes were isolated from sewage samples. These phages have a broad host range and effectively target 62.5% of the P. aeruginosa isolates with a positive correlation to the early isolates. All the phages have a virulence index of ≥0.90 (0.90–0.98), and one has a large burst size of 331 PFU/cell and a latency period of 30 min. All phages are stable under a wide range of temperature and pH conditions. Genomic analysis suggests the four phages are strictly lytic and devoid of identifiable temperate phage repressors and genes associated with antibiotic resistance and virulence. More significantly, two of the phages significantly delayed the onset of larval death when evaluated in a lethal Galleria mellonella infection model, suggesting their promise as phage therapy candidates for P. aeruginosa infections. Full article
(This article belongs to the Special Issue Phage-Bacteria Interplay: Phage Biology and Phage Therapy)
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17 pages, 3411 KiB  
Article
Salmonella Phage vB_SpuM_X5: A Novel Approach to Reducing Salmonella Biofilms with Implications for Food Safety
by Xinxin Jin, Xiuxiu Sun, Qin Lu, Zui Wang, Zhenggang Zhang, Xiaochun Ling, Yunpeng Xu, Ruiqin Liang, Junjie Yang, Li Li, Tengfei Zhang, Qingping Luo and Guofu Cheng
Microorganisms 2024, 12(12), 2400; https://doi.org/10.3390/microorganisms12122400 - 22 Nov 2024
Viewed by 1225
Abstract
Salmonella, a prevalent foodborne pathogen, poses a significant social and economic strain on both food safety and public health. The application of phages in the control of foodborne pathogens represents an emerging research area. In this study, Salmonella pullorum phage vB_SpuM_X5 (phage [...] Read more.
Salmonella, a prevalent foodborne pathogen, poses a significant social and economic strain on both food safety and public health. The application of phages in the control of foodborne pathogens represents an emerging research area. In this study, Salmonella pullorum phage vB_SpuM_X5 (phage X5) was isolated from chicken farm sewage samples. The results revealed that phage X5 is a novel Myoviridae phage. Phage X5 has adequate temperature tolerance (28 °C–60 °C), pH stability (4–12), and a broad host range of Salmonella bacteria (87.50% of tested strains). The addition of phage X5 (MOI of 100 and 1000) to milk inoculated with Salmonella reduced the number of Salmonella by 0.72 to 0.93 log10 CFU/mL and 0.66 to 1.06 log10 CFU/mL at 4 °C and 25 °C, respectively. The addition of phage X5 (MOI of 100 and 1000) to chicken breast inoculated with Salmonella reduced bacterial numbers by 1.13 to 2.42 log10 CFU/mL and 0.81 to 1.25 log10 CFU/mL at 4 °C and 25 °C, respectively. Phage X5 has bactericidal activity against Salmonella and can be used as a potential biological bacteriostatic agent to remove mature biofilms of Salmonella or for the prevention and control of Salmonella. Full article
(This article belongs to the Special Issue Phage-Bacteria Interplay: Phage Biology and Phage Therapy)
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Review

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22 pages, 4721 KiB  
Review
Bacteriophages: A Challenge for Antimicrobial Therapy
by Nallelyt Segundo-Arizmendi, Dafne Arellano-Maciel, Abraham Rivera-Ramírez, Adán Manuel Piña-González, Gamaliel López-Leal and Efren Hernández-Baltazar
Microorganisms 2025, 13(1), 100; https://doi.org/10.3390/microorganisms13010100 - 7 Jan 2025
Cited by 1 | Viewed by 2689
Abstract
Phage therapy, which involves the use of bacteriophages (phages) to combat bacterial infections, is emerging as a promising approach to address the escalating threat posed by multidrug-resistant (MDR) bacteria. This brief review examines the historical background and recent advancements in phage research, focusing [...] Read more.
Phage therapy, which involves the use of bacteriophages (phages) to combat bacterial infections, is emerging as a promising approach to address the escalating threat posed by multidrug-resistant (MDR) bacteria. This brief review examines the historical background and recent advancements in phage research, focusing on their genomics, interactions with host bacteria, and progress in medical and biotechnological applications. Additionally, we expose key aspects of the mechanisms of action, and therapeutic uses of phage considerations in treating MDR bacterial infections are discussed, particularly in the context of infections related to virus–bacteria interactions. Full article
(This article belongs to the Special Issue Phage-Bacteria Interplay: Phage Biology and Phage Therapy)
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