Molecular Ecology of Microalgae and Cyanobacteria

A special issue of Microorganisms (ISSN 2076-2607). This special issue belongs to the section "Environmental Microbiology".

Deadline for manuscript submissions: closed (31 August 2024) | Viewed by 1043

Special Issue Editors


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Guest Editor
Department of Biology, Institute for Plant Sciences, University of Cologne, Zuelpicher Str. 47B, 50674 Cologne, Germany
Interests: environmental microbiology; polar ecology; microbial cell biology; cyanobacteria; micro algae

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Guest Editor
Institute of Biological Sciences, Applied Ecology and Phycology, University of Rostock, Albert-Einstein-Straße 3, 18059 Rostock, Germany
Interests: microalgae; biocrusts; ecology; biodiversity; ecophysiology

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Guest Editor
Department of Biology, Botanical Institute, University of Cologne, Zulpicher Str. 47B, 50674 Cologne, Germany
Interests: microbial ecology; environmental microbiology; microbial diversity; soil; ecology; microbiology; molecular biology; community structure; biodiversity; climate change

Special Issue Information

Dear Colleagues,

Molecular omics methods are dramatically changing the field of ecology, allowing research questions and hypotheses to be tested in situ that would have been impossible to answer just a few years ago. Some often overlooked components of marine, freshwater, and terrestrial habitats are microalgae and cyanobacteria, found almost everywhere on Earth, from nearly all marine and freshwater habitats to cold polar deserts and tropical rainforests. Their lifestyles can also vary dramatically, from free-living forms, epiphytic forms growing on trees, symbiotic forms living inside other organisms to endolithic forms. They are important primary producers and nitrogen fixers in many ecosystems. Even today, we know little about these organisms' diversity (intra- and interspecific) and how they survive the sometimes extreme conditions of their specific habitat. This Special Issue aims to bring together scientists using modern omics approaches to understand the ecology/biology of these amazing organisms.

Dr. Burkhard Becker
Prof. Dr. Karin Glaser
Dr. Ekaterina Pushkareva
Guest Editors

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Keywords

  • microalgae
  • cyanobacteria
  • molecular omics

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Published Papers (1 paper)

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Research

24 pages, 2645 KiB  
Article
The Diatom Diversity and Ecological Status of a Tufa-Depositing River through eDNA Metabarcoding vs. a Morphological Approach—A Case Study of the Una River (Bosnia and Herzegovina)
by Jasmina Kamberović, Marija Gligora Udovič, Antonija Kulaš, Kálmán Tapolczai, Sandi Orlić, Amela Jusufović, Almina Gajić, Petar Žutinić, Adisa Ahmić and Belma Kalamujić Stroil
Microorganisms 2024, 12(8), 1722; https://doi.org/10.3390/microorganisms12081722 - 21 Aug 2024
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Abstract
Tufa deposits in karst rivers are unique habitats created by mutual interactions between specific environmental and biotope features and inhabited by diatoms as a highly abundant and diverse algal group. This pilot study aimed to investigate the diversity of diatom communities on tufa [...] Read more.
Tufa deposits in karst rivers are unique habitats created by mutual interactions between specific environmental and biotope features and inhabited by diatoms as a highly abundant and diverse algal group. This pilot study aimed to investigate the diversity of diatom communities on tufa depositing habitats and assess the Una River’s ecological status using a comparative molecular and morphological approach for diatom identification. The 312 base pairs of the rbcL gene were barcoded and analyzed using MiSeq reads and amplicon sequence variants (ASVs) obtained by the DADA2 pipeline. The reference database Diat.barcode v7 was used for taxonomic assignment. The morphological identification of the diatoms was carried out in parallel. In total, the combined dataset revealed 46 taxa identified at genus rank, 125 on the subgenus, and 145 on combined taxonomy rank. The metabarcoding approach mostly leads to a lower number of identified taxa at species rank (58 in molecular vs. 119 in optical inventory), resulting in higher values of beta diversity and heterogeneity in diatom assemblages in samples obtained by morphological approach. Despite the high percentage of taxonomically not assigned diatom ASVs to the species rank, high Shannon diversity index values and a similar number of taxa per locations compared to the morphological approach were obtained. Taxa Achnanthidium minutissimum (Kützing) Czarnecki, Achnanthidium pyrenaicum (Hustedt) H.Kobayasi, Amphora pediculus (Kützing) Grunow, Diatoma vulgaris Bory, Navicula cryptotenella Lange-Bertalot, and Navicula tripunctata (O.F.Müller) Bory were identified at all locations in both inventories. Although limited consistency in the diatom abundances between the two inventory datasets was found, a similar grouping of samples was observed connected to the river’s longitudinal gradient. The data obtained using molecular approach in most sites indicated a mostly lower ecological status (good or moderate) compared to the data obtained from the morphological approach (high, good, and moderate). The potential of environmental DNA (eDNA) diatom metabarcoding for water monitoring and diversity studies is undeniable, but to fully realize the benefits of these methods in the future, it is essential to standardize protocols and expand the reference database for species found in specific habitats, such as tufa deposits. Full article
(This article belongs to the Special Issue Molecular Ecology of Microalgae and Cyanobacteria)
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