Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
7.4
4.1
Journals
Microorganisms
Special Issues
Infection and Transmission of Clinical Pathogens in Livestock
share announcement

Infection and Transmission of Clinical Pathogens in Livestock

A special issue of Microorganisms (ISSN 2076-2607). This special issue belongs to the section "Veterinary Microbiology".

Deadline for manuscript submissions: closed (15 March 2024) | Viewed by 3863

Special Issue Editor

Prof. Dr. Ludwig Eduard Hölzle

E-Mail Website
Guest Editor
Institute of Animal Sciences, Department of Livestock Infectiology and Environmental Hygiene, University of Hohenheim, Garbenstarsse 30, 70599 Stuttgart, Germany
Interests: Infection biology; persistent infections; disinfection; animal hygiene; environmental hygiene

Special Issue Information

Dear Colleagues,

The societal impact of infectious diseases in livestock and their zoonotic potential has increased over the last decade. Within this, the development of novel pathogens or pathogens with novel properties (host spectrum, reservoirs, antimicrobial resistances) is of high attendance. Understanding the transmission of these pathogens within and around the animal husbandry can largely influence the significance and distribution of infectious diseases in livestock animals and therefore, also the development and spread of zoonotic pathogens (One Health).

This Special Issue aims to gather up-to-date research on reservoirs (better understanding of how pathogens survive both in livestock animals as well as in the environment (including reservoir hosts and vectors), transmission pathways and environmental properties of infectious agents (bacteria, viruses, parasites, fungi). Moreover, work dealing with mitigation strategies to minimize the transmission of infectious agents are very welcome (e.g. disinfection, alternative methods).

  • Livestock infectious agents (including zoonotic pathogens)
  • Molecular and cellular mechanisms of infections (esp. persistent infections)
  • The role of the environment and as reservoirs in disease transmission (including vectors)
  • Airborne, waterborne, feedborne infectious diseases in livestock
  • Tenacity of pathogens
  • Disinfection
  • Sanitation of biomass

Prof. Dr. Ludwig Eduard Hölzle
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Microorganisms is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • livestock pathogens
  • one health
  • transmission
  • disinfection
  • persistent Infections
  • vectors
  • environment
  • reservoirs

Published Papers (4 papers)

Download All Papers
Order results
Result details
Show export options expand_more Show export options expand_less
Select all
Export citation of selected articles as:

Research

Jump to: Review

15 pages, 2662 KiB  
Article
Effects of Enterotoxigenic Escherichia coli Challenge on Jejunal Morphology and Microbial Community Profiles in Weaned Crossbred Piglets
by Juan Xu, Zhen Jia, Shu Xiao, Cimin Long and Leli Wang
Microorganisms 2023, 11(11), 2646; https://doi.org/10.3390/microorganisms11112646 - 27 Oct 2023
Viewed by 1100
Abstract
Pathogenic enterotoxigenic Escherichia coli (ETEC) is a major cause of bacterial diarrhea in weaning piglets, which are vulnerable to changes in environment and feed. This study aimed to determine the effects of the ETEC challenge on piglet growth performance, diarrhea rate, jejunal microbial [...] Read more.
Pathogenic enterotoxigenic Escherichia coli (ETEC) is a major cause of bacterial diarrhea in weaning piglets, which are vulnerable to changes in environment and feed. This study aimed to determine the effects of the ETEC challenge on piglet growth performance, diarrhea rate, jejunal microbial profile, jejunal morphology and goblet cell distribution. A total of 13 piglets from one litter were selected on postnatal day 21 and assigned to treatments with or without ETEC challenge at 1 × 108 CFUs, as ETEC group or control group, respectively. On postnatal day 28, samples were collected, followed by the detection of serum biochemical indexes and inflammatory indicators, HE staining, PAS staining and 16S rDNA gene amplicon sequencing. Results showed that the growth performance decreased, while the diarrhea rate increased for the ETEC group. The jejunum is the main segment of the injured intestine during the ETEC challenge. Compared with the control, the ETEC group displayed fewer goblet cells in the jejunum, where goblet cells are more distributed at the crypt and less distributed at the villus. In addition, ETEC piglets possessed higher abundances of the genus Desulfovibrio, genus Oxalobacter and genus Peptococus and lower abundances of the genus Prevotella 2, genus Flavonifractor and genus Blautra. In terms of alpha diversity, Chao 1 and observed features indexes were both increased for the ETEC group. Our study provides insights into jejunal histopathological impairment and microbial variation in response to ETEC infection for weaned piglets and is a valuable reference for researchers engaged in animal health research to select stress models. Full article
(This article belongs to the Special Issue Infection and Transmission of Clinical Pathogens in Livestock)
►▼ Show Figures

Figure 1

10 pages, 2506 KiB  
Article
Development of a Real-Time Quantitative PCR Based on a TaqMan-MGB Probe for the Rapid Detection of Theileria haneyi
by Bingqian Zhou, Guangpu Yang, Zhe Hu, Kewei Chen, Wei Guo, Xiaojun Wang and Cheng Du
Microorganisms 2023, 11(11), 2633; https://doi.org/10.3390/microorganisms11112633 - 26 Oct 2023
Viewed by 943
Abstract
Equine piroplasmosis (EP) is a parasitic disease caused by Theileria equi (T. equi), Babesia caballi (B. caballi) and Theileria haneyi (T. haneyi). This disease is considered to be reportable by the World Organization for Animal Health (WOAH). [...] Read more.
Equine piroplasmosis (EP) is a parasitic disease caused by Theileria equi (T. equi), Babesia caballi (B. caballi) and Theileria haneyi (T. haneyi). This disease is considered to be reportable by the World Organization for Animal Health (WOAH). Real-time quantitative PCR (qPCR) is regarded as a straightforward, rapid and sensitive diagnostic method to detect pathogens. However, qPCR has not been employed in the various epidemiological investigations of T. haneyi. In this study, we developed a new qPCR method to detect T. haneyi based on the chr1sco (chromosome 1 single-copy open reading frame (ORF)) gene, which has no detectable orthologs in T. equi or B. caballi. A TaqMan MGB probe was used in the development of the qPCR assay. A plasmid containing the chr1sco gene was constructed and used to establish the standard curves. The novel qPCR technique demonstrated great specificity for detecting additional frequent equine infectious pathogens and sensitivity for detecting diluted standard plasmids. This qPCR was further validated by comparison with an optimized nested PCR (nPCR) assay in the analysis of 96 clinical samples. The agreement between the nPCR assay and the established qPCR assay was 85.42%. The newly established method could contribute to the accurate diagnosis of T. haneyi infections in horses. Full article
(This article belongs to the Special Issue Infection and Transmission of Clinical Pathogens in Livestock)
►▼ Show Figures

Figure 1

16 pages, 6692 KiB  
Article
A Novel Rhipicephalus microplus Estrogen Related Receptor (RmERR), a Molecular and In Silico Characterization of a Potential Protein Binding Estrogen
by Hugo Aguilar-Díaz, Rosa Estela Quiroz-Castañeda, Ixchel Guadalupe Díaz-Esquivel, Raquel Cossío-Bayúgar and Estefan Miranda-Miranda
Microorganisms 2023, 11(9), 2294; https://doi.org/10.3390/microorganisms11092294 - 12 Sep 2023
Viewed by 907
Abstract
The search for targets to control ticks and tick-borne diseases has been an ongoing problem, and so far, we still need efficient, non-chemical alternatives for this purpose. This search must consider new alternatives. For example genomics analysis is a widely applied tool in [...] Read more.
The search for targets to control ticks and tick-borne diseases has been an ongoing problem, and so far, we still need efficient, non-chemical alternatives for this purpose. This search must consider new alternatives. For example genomics analysis is a widely applied tool in veterinary health studies to control pathogens. On the other hand, we propose that regulation of endocrine mechanisms represents a feasible alternative to biologically controlling tick infestations. Thus, we performed the molecular identification of an estrogen-related receptor gene of Rhipicephalus microplus called RmERR by RT-PCR in tick ovaries, embryonic cells, and hemolymph, which allowed us to analyze its expression and propose potential functions in endocrine mechanisms and developmental stages. In addition, we performed an in silico characterization to explore the molecular interactions of RmERR with different estrogens, estrogenic antagonists, and endocrine disruptor Bisphenol A (BPA), finding potential interactions predicted by docking analysis and supported by negative values of ΔG (which suggests the potential interaction of RmERR with the molecules evaluated). Additionally, phylogenetic reconstruction revealed that RmERR is grouped with other tick species but is phylogenetically distant from host vertebrates’ ERRs. In summary, this study allowed for the identification of an ERR in cattle tick R. microplus for the first time and suggested its interaction with different estrogens, supporting the idea of a probable transregulation process in ticks. The elucidation of this interaction and its mechanisms unveiled its potential as a target to develop tick control strategies. Full article
(This article belongs to the Special Issue Infection and Transmission of Clinical Pathogens in Livestock)
►▼ Show Figures

Figure 1

Review

Jump to: Research

14 pages, 1497 KiB  
Review
Hemotrophic Mycoplasmas—Vector Transmission in Livestock
by Mareike Arendt, Julia Stadler, Mathias Ritzmann, Julia Ade, Katharina Hoelzle and Ludwig E. Hoelzle
Microorganisms 2024, 12(7), 1278; https://doi.org/10.3390/microorganisms12071278 - 23 Jun 2024
Viewed by 432
Abstract
Hemotrophic mycoplasmas (HMs) are highly host-adapted and specialized pathogens infecting a wide range of mammals including farm animals, i.e., pigs, cattle, sheep, and goats. Although HMs have been known for over 90 years, we still do not know much about the natural transmission [...] Read more.
Hemotrophic mycoplasmas (HMs) are highly host-adapted and specialized pathogens infecting a wide range of mammals including farm animals, i.e., pigs, cattle, sheep, and goats. Although HMs have been known for over 90 years, we still do not know much about the natural transmission routes within herds. Recently, it has been repeatedly discussed in publications that arthropod vectors may play a role in the transmission of HMs from animal to animal. This is mainly since several HM species could be detected in different potential arthropod vectors by PCR. This review summarizes the available literature about the transmission of bovine, porcine, ovine, and caprine HM species by different hematophagous arthropod vectors. Since most studies are only based on the detection of HMs in potential vectors, there are rare data about the actual vector competence of arthropods. Furthermore, there is a need for additional studies to investigate, whether there are biological vectors in which HMs can multiply and be delivered to new hosts. Full article
(This article belongs to the Special Issue Infection and Transmission of Clinical Pathogens in Livestock)
►▼ Show Figures

Figure 1

Show export options expand_more Show export options expand_less
Select all
Export citation of selected articles as:
 
Displaying articles 1-4
Back to TopTop