Bacterial Functions in Carbon, Nitrogen, and Sulfur Cycles

The climate is changing. The average temperature in Wuhan, China, is forecast to increase by at least 4.5 °C over the next century. Shallow lakes are important components of the biosphere, but they are sensitive to climate change and nutrient pollution. We hypothesized that nutrient concentration is the key determinant of nutrient fluxes at the water-sediment interface, and that increased temperature increases nutrient movement to the water column because warming stimulates shifts in microbial composition and function. Here, twenty-four mesocosms, mimicking shallow lake ecosystems, were used to study the effects of warming by 4.5 °C above ambient temperature at two levels of nutrients relevant to current degrees of lake eutrophication levels. This study lasted for 7 months (April–October) under conditions of near-natural light. Intact sediments from two different trophic lakes (hypertrophic and mesotrophic) were used, separately. Environmental factors and bacterial community compositions of overlying water and sediment were measured at monthly intervals (including nutrient fluxes, chlorophyll a [chl a], water conductivity, pH, sediment characteristics, and sediment-water et al.). In low nutrient treatment, warming significantly increased chl a in the overlying waters and bottom water conductivity, it also drives a shift in microbial functional composition towards more conducive sediment carbon and nitrogen emissions. In addition, summer warming significantly accelerates the release of inorganic nutrients from the sediment, to which microorganisms make an important contribution. In high nutrient treatment, by contrast, the chl a was significantly decreased by warming, and the nutrient fluxes of sediment were significantly enhanced, warming had considerably smaller effects on benthic nutrient fluxes. Our results suggest that the process of eutrophication could be significantly accelerated in current projections of global warming, especially in shallow unstratified clear-water lakes dominated by macrophytes. Full article

Poultry litter is a valuable crude protein feedstuff for ruminants, but it must be treated to kill pathogens before feeding. Composting effectively kills pathogens, but it risks losing ammonia to volatilization or leaching during degradation of uric acid and urea. Hops bitter acids also exert antimicrobial activity against certain pathogenic and nitrogen-degrading microbes. Consequently, the present studies were conducted to test if adding bitter acid-rich hop preparations to simulated poultry litter composts may improve nitrogen retention while simultaneously improving pathogen killing. Results from an initial study, testing doses of Chinook or Galena hops preparations designed to each deliver 79 ppm hops β-acid, revealed that, after nine days simulated composting of wood chip litter, ammonia concentrations were 14% lower (p < 0.05) in Chinook-treated composts than untreated composts (13.4 ± 1.06 µmol/g). Conversely, urea concentrations were 55% lower (p < 0.05) in Galena-treated than untreated composts (6.2 ± 1.72 µmol/g). Uric acid accumulations were unaffected by hops treatments in this study but were higher (p < 0.05) after three days than after zero, six, or nine days of composting. In follow-up studies, Chinook or Galena hops treatments (delivering 2042 or 6126 ppm of β-acid, respectively) for simulated composts (14 days) of wood chip litter alone or mixed 3:1 with ground Bluestem hay (Andropogon gerardii) revealed that these higher dosages had little effect on ammonia, urea, or uric acid accumulations when compared to untreated composts. Volatile fatty acid accumulations measured in these later studies were affected by the hops treatments, with butyrate accumulations being lower after 14 days in hops-treated composts than in untreated compost. In all studies, beneficial effects of Galena or Chinook hops treatments were not observed on the antimicrobial activity of the simulated composts, with composting by itself decreasing (p < 0.05) counts of select microbial populations by more than 2.5 log₁₀ colony forming units/g compost dry matter. Thus, while hops treatments had little effect on pathogen control or nitrogen retention within the composted litter, they did lessen accumulations of butyrate, which may prevent adverse effects of this fatty acid on palatability of litter fed to ruminants. Full article

Microorganisms acting as sinks for the greenhouse gas nitrous oxide (N₂O) are gaining increasing attention in the development of strategies to control N₂O emissions. Non-denitrifying N₂O reducers are of particular interest because they can provide a real sink without contributing to N₂O release. The bacterial strain under investigation (IGB 4-14^T), isolated in a mesocosm experiment to study the litter decomposition of Phragmites australis (Cav.), is such an organism. It carries only a nos gene cluster with the sec-dependent Clade II nosZ and is able to consume significant amounts of N₂O under anoxic conditions. However, consumption activity is considerably affected by the O₂ level. The reduction of N₂O was not associated with cell growth, suggesting that no energy is conserved by anaerobic respiration. Therefore, the N₂O consumption of strain IGB 4-14^T rather serves as an electron sink for metabolism to sustain viability during transient anoxia and/or to detoxify high N₂O concentrations. Phylogenetic analysis of 16S rRNA gene similarity revealed that the strain belongs to the genus Flavobacterium. It shares a high similarity in the nos gene cluster composition and the amino acid similarity of the nosZ gene with various type strains of the genus. However, phylogenomic analysis and comparison of overall genome relatedness indices clearly demonstrated a novel species status of strain IGB 4-14^T, with Flavobacterium lacus being the most closely related species. Various phenotypic differences supported a demarcation from this species. Based on these results, we proposed a novel species Flavobacterium azooxidireducens sp. nov. (type strain IGB 4-14^T = LMG 29709^T = DSM 103580^T). Full article

Despite the central role of microorganisms in soil fertility, little understanding exists regarding the impact of management practices and soil microbial diversity on soil processes. Strong correlations among soil microbial composition, management practices, and microbially mediated processes have been previously shown. However, limited integration of the different parameters has hindered our understanding of agroecosystem functioning. Multivariate analyses of these systems allow simultaneous evaluation of the parameters and can lead to hypotheses on the microbial groups involved in specific nutrient transformations. In the present study, using a multivariate approach, we investigated the effect of microbial composition (16SrDNA sequencing) and soil properties in carbon mineralization (C_MIN) (BIOLOG™, Hayward, CA, USA) across different management categories on coffee agroecosystems in Mexico. Results showed that (i) changes in soil physicochemical variables were related to management, not to region, (ii) microbial composition was associated with changes in management intensity, (iii) specific bacterial groups were associated with different management categories, and (iv) there was a broader utilization range of carbon sources in non-managed plots. The identification of specific bacterial groups, management practices, and soil parameters, and their correlation with the utilization range of carbon sources, presents the possibility to experimentally test hypotheses on the interplay of all these components and further our understanding of agroecosystem functioning and sustainable management. Full article

Lake Chitu is a highly productive soda lake found in the East African Rift Valley, where Arthrospira fusiformis (Spirulina platensis) is the main primary producer. High biomass accumulation requires an adequate supply of nitrogen. However, Lake Chitu is a closed system without any external nutrient input. A recent study has also demonstrated the presence of a diverse group of denitrifying bacteria, indicating a possible loss of nitrate released from the oxidation of organic matter. The aim of this study was to isolate culturable nitrogen-fixing alkaliphiles and evaluate their potential contribution in the nitrogen economy of the soda lake. A total of 118 alkaliphiles belonging to nine different operational taxonomic units (OTUs) were isolated using a nitrogen-free medium. Nineteen isolates were tested for the presence of the nifH gene, and 11 were positive. The ability to fix nitrogen was tested by co-culturing with a non-nitrogen-fixing alkaliphile, Alkalibacterium sp. 3.5*R1. When inoculated alone, Alkalibacterium sp. 3.5*R1 failed to grow on a nitrogen-free medium, but grew very well when co-cultured with the nitrogen-fixing alkaliphile NF10m6 isolated in this study, indicating the availability of nitrogen. These results show that nitrogen fixation by alkaliphiles may have an important contribution as a source of nitrogen in soda lakes. Full article

Denitrification and anammox occur widely in aquatic ecosystems serving vital roles in nitrogen pollution removal. However, small waterbodies are sensitive to external influences; stormwater runoff carrying nutrients and oxygen, flows into waterbodies resulting in a disruption of geochemical and microbial processes. Nonetheless, little is known about how these short-term external inputs affect the microbial processes of nitrogen removal in small waterbodies. To investigate the effects of NO₃⁻, NH₄⁺, dissolved oxygen (DO) and organic C on microbial nitrogen removal in pond sediments, regulation experiments have been conducted using slurry incubation experiments and ¹⁵N tracer techniques in this study. It was demonstrated the addition of NO₃⁻ (50 to 800 μmol L⁻¹) significantly promoted denitrification rates, as expected by Michaelis-Menten kinetics. Ponds with higher NO₃⁻ concentrations in the overlying water responded more greatly to NO₃⁻ additions. Moreover, N₂O production was also promoted by such an addition of NO₃⁻. Denitrification was significantly inhibited by the elevation of DO concentration from 0 to 2 mg L⁻¹, after which no significant increase in inhibition was observed. Denitrification rates increased when organic C was introduced. Due to the abundant NH₄⁺ in pond sediments, the addition demonstrated little influence on nitrogen removal. Moreover, anammox rates showed no significant changes to any amendment. Full article

Microbially induced calcium carbonate precipitation (MICP) has been highlighted for its application in civil engineering, and in the environmental and geotechnical fields. Ureolytic activity is one of the most promising bacterial mechanisms in terms of inducing calcium carbonate formation. In this study, four bacterial isolates with high-yield urease production capabilities were obtained from two-step screening using a high-buffered urea medium. The highest urease activity and calcium carbonate formation was observed in Lysinibacillus fusiformis 5.1 with 4.40 × 10³ unit/L of urease and 24.15 mg/mL of calcium carbonate, followed by Lysinibacillus xylanilyticus 4.3 with 3.93 × 10³ unit/L of urease and 22.85 mg/mL of calcium carbonate. The microstructure of the precipitated crystalline calcium carbonate was observed using scanning electron microscopy. X-ray diffraction analysis confirmed that the main polymorph of the calcium carbonate particle obtained from both isolates was calcite. Examination of the material-crack filling in mortar specimens showed that calcite layers had formed along the crack edges and inside after 10 days, and gradually filled the cracks up to the upper surface. These results showed that these two isolates presented robust characteristics of potential MICP-inducing bacteria for civil engineering and material engineering applications. Full article

Benthic microbial communities of intertidal zones perform important biogeochemical processes and provide accessible nutrients for higher organisms. To unravel the ecosystem services of salt marsh microbial communities, we analyzed bacterial diversity and metabolic potential along the land–sea transition zone on seasonal scales on the German North Sea Island of Spiekeroog. Analysis of bacterial community was based on amplicon sequencing of 16S rRNA genes and –transcripts. Insights into potential community function were obtained by applying the gene prediction tool tax4fun2. We found that spatial variation of community composition was greater than seasonal variations. Alphaproteobacteria (15%), Gammaproteobacteria (17%) and Planctomycetes (11%) were the most abundant phyla across all samples. Differences between the DNA-based resident and RNA-based active communities were most pronounced within the Planctomycetes (17% and 5%) and Cyanobacteriia (3% and 12%). Seasonal differences were seen in higher abundance of Gammaproteobacteria in March 2015 (25%) and a cyanobacterial summer bloom, accounting for up to 70% of the active community. Taxonomy-based prediction of function showed increasing potentials for nitrification, assimilatory nitrate and sulfate reduction from sea to land, while the denitrification and dissimilatory sulfate reduction increased towards the sea. In conclusion, seasonal differences mainly occurred by blooming of individual taxa, while the overall community composition strongly corresponded to locations. Shifts in their metabolism could drive the salt marsh’s function, e.g., as a potential nitrogen sink. Full article