Advances in Microbial Endophytes Omics and Fungal Biocontrol Agents

A special issue of Microorganisms (ISSN 2076-2607). This special issue belongs to the section "Plant Microbe Interactions".

Deadline for manuscript submissions: closed (30 July 2021) | Viewed by 3624

Special Issue Editor


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Guest Editor
Department of Food and Bioproduct Sciences, University of Saskatchewan, Saskatoon, SK S7N 5A8, Canada
Interests: molecular mycology and biotechnology; microbiome and endosymbionts; biocontrol; mycotoxins

Special Issue Information

Dear Colleagues,

Despite an accelerated pace of research surrounding the endophytic microbiome, this fascinating minute world bears many open questions, waiting for scientific resolutions. Understanding how beneficial endophytic and biocontrol microorganisms can counter climate change and invasive plant pathogens is the greatest challenge of forestry, agriculture, and plant biotechnology. Scientific innovation can benefit from deciphering endophytic and fungal biocontrol agents’ lifestyles. The plant microbiomes—endophytes and biocontrol microbes—have the ability to colonize a plant's internal tissues, shaping plant traits and interactome, as well as ensuring protection from abiotic and biotic stresses, including diseases, insect pests, and pathogenic nematodes. Despite these attributes, a profound understanding of the genomic, transcriptomic and proteomics drivers is lacking, impeding advancement of knowledge on core mechanisms of metagenomic-multitrophic interactions linked to plant resistance, host plant protection, and environmental sustainability.

The aim of this Special Issue, entitled “Advancements in Microbial Endophytes Omics and Fungal Biocontrol Agents”, is to explore recent innovations, theoretical concepts and contemporary experimental models, surrounding true endophytic and biocontrol modes of actions (e.g. hyper-parasitism and predation), which are linked to the direct and indirect promotion of plant growth, physiology, and resistance.

Methodological barriers are lessening; and general revival in omics, imaging, and computational is rising to help address these scientific priorities.

I invite contributions from scientists, microbiologists or mycologists to this Special Issue in order to highlight innovative ideas and experimental results, which empower microbial-based plant technologies to improve plant, environmental, animal and human health.

Dr. Vladimir Vujanovic
Guest Editor

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Keywords

  • plant endophytes
  • biological control
  • microbiome/mycobiome
  • omics, antimicrobials
  • microbe-mediated traits
  • microbe-driven biotechnology
  • imaging

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Published Papers (1 paper)

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Research

21 pages, 3662 KiB  
Article
RNA-Seq Provides New Insights into the Gene Expression Changes in Azoarcus olearius BH72 under Nitrogen-Deficient and Replete Conditions beyond the Nitrogen Fixation Process
by Shanmugam Solaiyappan Mani and Barbara Reinhold-Hurek
Microorganisms 2021, 9(9), 1888; https://doi.org/10.3390/microorganisms9091888 - 6 Sep 2021
Cited by 5 | Viewed by 3178
Abstract
Azoarcus olearius BH72 is an endophyte capable of biological nitrogen fixation (BNF) and of supplying nitrogen to its host plant. Our previous microarray approach provided insights into the transcriptome of strain BH72 under N2-fixation in comparison to ammonium-grown conditions, which already [...] Read more.
Azoarcus olearius BH72 is an endophyte capable of biological nitrogen fixation (BNF) and of supplying nitrogen to its host plant. Our previous microarray approach provided insights into the transcriptome of strain BH72 under N2-fixation in comparison to ammonium-grown conditions, which already indicated the induction of genes not related to the BNF process. Due to the known limitations of the technique, we might have missed additional differentially expressed genes (DEGs). Thus, we used directional RNA-Seq to better comprehend the transcriptional landscape under these growth conditions. RNA-Seq detected almost 24% of the annotated genes to be regulated, twice the amount identified by microarray. In addition to confirming entire regulated operons containing known DEGs, the new approach detected the induction of genes involved in carbon metabolism and flagellar and twitching motility. This may support N2-fixation by increasing energy production and by finding suitable microaerobic niches. On the other hand, energy expenditures were reduced by suppressing translation and vitamin biosynthesis. Nonetheless, strain BH72 does not appear to be content with N2-fixation but is primed for alternative economic N-sources, such as nitrate, urea or amino acids; a strong gene induction of machineries for their uptake and assimilation was detected. RNA-Seq has thus provided a better understanding of a lifestyle under limiting nitrogen sources by elucidating hitherto unknown regulated processes. Full article
(This article belongs to the Special Issue Advances in Microbial Endophytes Omics and Fungal Biocontrol Agents)
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