Staphylococcus aureus: Host Interactions and Adaptation

A special issue of Microorganisms (ISSN 2076-2607). This special issue belongs to the section "Medical Microbiology".

Deadline for manuscript submissions: 30 September 2024 | Viewed by 1177

Special Issue Editors


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Guest Editor
Department of Surgery, School of Medicine, The University of Auckland, Auckland 1023, New Zealand
Interests: sinusitis; bacteria; staphylococcus aureus; pathogenesis; rhinology; endoscopic surgery; ENT; antimicrobials; medical microbiology; molecular bacteriology

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Guest Editor
Department of Surgery, School of Medicine, The University of Auckland, Auckland 1023, New Zealand
Interests: microbiology; host-microbe interactions; sinusitis; biomarkers; microbiome

Special Issue Information

Dear Colleagues,

Staphylococcus aureus, a versatile and notorious pathogen, remains a significant threat in healthcare settings and communities worldwide due to its ability to cause a diverse array of infections, ranging from skin and soft-tissue infections to life-threatening conditions, such as bacteremia and endocarditis. The intricate interplay between S. aureus and the host immune system, along with the pathogen's remarkable adaptability, presents a complex and dynamic landscape for research. Understanding the molecular mechanisms underlying S. aureus pathogenesis, virulence factors, antibiotic resistance, biofilm formation, and immune evasion strategies is crucial for developing novel therapeutic interventions and combating the challenges posed by this pathogen.

This Special Issue aims to delve into the cutting-edge research on the host interactions and adaptations of Staphylococcus aureus, inviting researchers to contribute their latest findings and insights to advance our understanding of this clinically significant pathogen. Topics of interest include the following:

  • Virulence factors: Exploration of the key virulence factors of S. aureus and their role in host colonization, immune evasion, and pathogenesis.
  • Antibiotic resistance: Investigation of the mechanisms driving antibiotic resistance in S. aureus and the development of novel therapeutic approaches with which to combat resistant strains.
  • Biofilm formation: Understanding biofilm formation by S. aureus and its impact on treatment outcomes and chronic infections.
  • Host immune response: Study of host immune responses to S. aureus infections, including immune evasion strategies employed by the pathogen.
  • Genomic adaptation: Analysis of genomic adaptations in S. aureus populations under selective pressures, shedding light on evolutionary dynamics and the emergence of new strains.

Prof. Dr. Richard George Douglas
Dr. Kristi Biswas
Guest Editors

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Keywords

  • Staphylococcus aureus (S. aureus)
  • virulence factors
  • pathogenesis
  • host interactions
  • biofilm formation
  • antibiotic resistance

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Published Papers (1 paper)

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Research

19 pages, 2657 KiB  
Article
Surface-Shaving of Staphylococcus aureus Strains and Quantitative Proteomic Analysis Reveal Differences in Protein Abundance of the Surfaceome
by Anders Karlsson, Leonarda Achá Alarcón, Beatriz Piñeiro-Iglesias, Gunnar Jacobsson, Susann Skovbjerg, Edward R. B. Moore, Pradeep Kumar Kopparapu, Tao Jin and Roger Karlsson
Microorganisms 2024, 12(8), 1725; https://doi.org/10.3390/microorganisms12081725 - 21 Aug 2024
Viewed by 276
Abstract
Staphylococcus aureus is a pathogen known to cause a wide range of infections. To find new targets for identification and to understand host–pathogen interactions, many studies have focused on surface proteins. We performed bacterial-cell surface-shaving, followed by tandem mass tag for quantitative mass [...] Read more.
Staphylococcus aureus is a pathogen known to cause a wide range of infections. To find new targets for identification and to understand host–pathogen interactions, many studies have focused on surface proteins. We performed bacterial-cell surface-shaving, followed by tandem mass tag for quantitative mass spectrometry proteomics, to examine the surfaceome of S. aureus. Two steps were performed, the first step including surface protein-deficient mutants of S. aureus Newman strain lacking important virulence genes (clfA and spa, important for adhesion and immune evasion and srtAsrtB, linking surface-associated virulence factors to the surface) and the second step including isolates of different clinical origin. All strains were compared to the Newman strain. In Step 1, altogether, 7880 peptides were identified, corresponding to 1290 proteins. In Step 2, 4949 peptides were identified, corresponding to 919 proteins and for each strain, approximately 20 proteins showed differential expression compared to the Newman strain. The identified surface proteins were related to host-cell-adherence and immune-system-evasion, biofilm formation, and survival under harsh conditions. The results indicate that surface-shaving of intact S. aureus bacterial strains in combination with quantitative proteomics is a useful tool to distinguish differences in protein abundance of the surfaceome, including the expression of virulence factors. Full article
(This article belongs to the Special Issue Staphylococcus aureus: Host Interactions and Adaptation)
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