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Recent Advances in Chromatography for Bioanalytical Applications

A special issue of Molecules (ISSN 1420-3049). This special issue belongs to the section "Analytical Chemistry".

Deadline for manuscript submissions: 15 December 2024 | Viewed by 1115

Special Issue Editors


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Guest Editor
Laboratory of Pharmaceutical Analysis, Division of Pharmaceutical Chemistry, School of Pharmacy, National and Kapodistrian University of Athens, Panepistimiopolis, Zografou, 15771 Athens, Greece
Interests: pharmaceutical analysis; analytical chemistry; analytical methods; drug analysis, metabolites; bioactive compounds; pharmaceuticals; cosmeceuticals
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Guest Editor
Laboratory of Analytical Chemistry, School of Chemistry, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece
Interests: analytical chemistry; sample preparation; chromatography; HPLC; method validation; method development; separation science; food analysis; bioanalysis; environmental analysis; green analytical chemistry; sorptive extraction; microextraction techniques
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Chromatography is the most powerful analytical technique that enables the separation, identification, and quantitation of various compounds in multicomponent chemical mixtures. The combination of chromatography with mass spectrometry enhances its capabilities and widens its applications across various analytical fields. Bioanalytical applications require the development of novel analytical procedures, including efficient sample collection and sample preparation protocols for the detection of compounds of interest at trace levels.

The target of this Special Issue is to present novel chromatographic methods using various sample collection and sample preparation techniques for the analysis of chemicals in multicomponent mixtures with applications in bioanalysis, toxicology, doping control, medicine, and food chemistry.

With your rich expertise in the field of analytical chemistry, we believe that your contribution to this Special Issue will definitely have a significant impact on the entire scientific research community.

Prof. Dr. Irene Panderi
Prof. Dr. Victoria Samanidou
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Molecules is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • chromatography
  • mass spectrometry
  • sample preparation
  • automated techniques
  • green analytical chemistry
  • bioanalysis
  • toxicology
  • doping control
  • food chemistry

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Published Papers (1 paper)

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Research

17 pages, 1322 KiB  
Article
Application of a Novel UPLC-MS/MS Method for Analysis of Rivaroxaban Concentrations in Dried Blood Spot and Plasma Samples Collected from Patients with Venous Thrombosis
by Kornel Pawlak, Łukasz Kruszyna, Marta Miecznikowska and Marta Karaźniewicz-Łada
Molecules 2024, 29(17), 4140; https://doi.org/10.3390/molecules29174140 - 31 Aug 2024
Viewed by 728
Abstract
Despite a higher safety profile compared to vitamin K antagonists, rivaroxaban therapy is still connected with multiple adverse effects, such as a high risk of bleeding. Thus, therapeutic drug monitoring (TDM) of rivaroxaban concentrations is suggested. An alternative to plasma samples can be [...] Read more.
Despite a higher safety profile compared to vitamin K antagonists, rivaroxaban therapy is still connected with multiple adverse effects, such as a high risk of bleeding. Thus, therapeutic drug monitoring (TDM) of rivaroxaban concentrations is suggested. An alternative to plasma samples can be dried blood spots (DBS), which minimize the cost of sample storage and transport. In this study, we developed a UPLC-MS/MS method for the analysis of rivaroxaban in DBS and plasma samples. Chromatographic separation was achieved on a Zorbax Eclipse Plus C18 column (2.1 × 100 mm; 3.5 µm, Agilent Technologies Inc., Santa Clara, CA, USA) with a mobile phase consisting of water and acetonitrile, both containing 0.1% formic acid. The analytes were detected using a positive ionization mode by multiple reaction monitoring. We validated the method according to ICH guidelines. The precision and accuracy were satisfactory. Extraction recovery was approximately 57% and 66% for DBS and plasma samples, respectively. A high correlation between rivaroxaban concentrations in plasma and DBS samples collected from patients was confirmed with Deming regression. The suitability of both sampling techniques for the rivaroxaban TDM was also verified by Bland–Altman plots based on DBS-predicted and observed plasma concentrations. In addition, we found a significant relationship between rivaroxaban concentrations and coagulation parameters, including prothrombin time (PT) and international normalized ratio (INR). Full article
(This article belongs to the Special Issue Recent Advances in Chromatography for Bioanalytical Applications)
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