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Field-Flow Fractionation in Chemical Biology
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Field-Flow Fractionation in Chemical Biology

A special issue of Molecules (ISSN 1420-3049). This special issue belongs to the section "Analytical Chemistry".

Deadline for manuscript submissions: closed (15 October 2022) | Viewed by 6774

Special Issue Editors

Prof. Dr. Pierluigi Reschiglian

E-Mail Website1 Website2
Guest Editor
1. Department of Chemistry G. Ciamician, University of Bologna, Bologna, Italy
2. byFlow Srl, Bologna, Italy
Interests: separation science; nanotechnologies; biotechnologies; technology transfer; academic entrepreneurship
Prof. Dr. Andrea Zattoni

E-Mail Website1 Website2
Guest Editor
1. Department of Chemistry G. Ciamician, University of Bologna, Bologna, Italy
2. byFlow Srl, Bologna, Italy
Interests: separation science; hyphenated analytical techniques; bio-nanosciences
Prof. Dr. Barbara Roda

E-Mail Website
Guest Editor
1. Department of Chemistry G. Ciamician, University of Bologna, Bologna, Italy
2. byFlow Srl, Bologna, Italy
Interests: separation sciences; bioanalytics; nanotechnology; cell separation
Dr. Valentina Marassi

E-Mail Website
Guest Editor
1. Department of Chemistry G. Ciamician, University of Bologna, Bologna, Italy
2. byFlow Srl, Bologna, Italy
Interests: separation science; field-flow fractionation; light scattering; multianalytical platforms; fate of nanomaterials; nanobiotechnology; nanomedicine; nanotechnological industry; nanotoxicity; biopharmaceutics

Special Issue Information

Dear Colleagues,

Field-flow fractionation is increasingly employed to assist the development, characterization, and purification of macromolecules of natural, biological, or synthetic origin. This flow-assisted separation technique is ideally suited to separate native structures with a gentle separation mechanism; the operational flexibility is appealing both for early-stage development of materials and for semipreparative purposes. More recently, its use for the analysis of nano-biopharmaceutical products has rapidly expanded. R&D, certification, validation, industrialization, and large-scale production require instrumental and methodological platforms specifically tailored to handle such analytes in native conditions. In this Special Issue, we want to collect the most recent contributions from researchers in field-flow fractionation and hyphenated techniques for the analysis and characterization of macromolecules, nanoparticles, and composite materials in biological systems, pharmaceutics, and chemical biology.

Prof. Dr. Pierluigi Reschiglian
Prof. Dr. Andrea Zattoni
Prof. Dr. Barbara Roda
Dr. Valentina Marassi
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Molecules is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • Field-flow fractionation
  • Chemical biology
  • Hyphenated techniques
  • Light scattering detection
  • Antibodies
  • Drug delivery
  • Virus-like particles
  • Polymer conjugates
  • Aggregation
  • Particle size distribution analysis
  • Drug stability and formulation

Published Papers (2 papers)

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Research

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14 pages, 2945 KiB  
Article
Application of Af4-Multidetection to Liraglutide in Its Formulation: Preserving and Representing Native Aggregation
by Valentina Marassi, Marco Macis, Stefano Giordani, Lucia Ferrazzano, Alessandra Tolomelli, Barbara Roda, Andrea Zattoni, Antonio Ricci, Pierluigi Reschiglian and Walter Cabri
Molecules 2022, 27(17), 5485; https://doi.org/10.3390/molecules27175485 - 26 Aug 2022
Cited by 8 | Viewed by 3829
Abstract
Aggregation is among the most critical parameters affecting the pharmacological and safety profile of peptide Active Pharmaceutical Ingredients (APIs). For this reason, it is of utmost importance to define the exact aggregation state of peptide drugs, particularly when the API is marketed as [...] Read more.
Aggregation is among the most critical parameters affecting the pharmacological and safety profile of peptide Active Pharmaceutical Ingredients (APIs). For this reason, it is of utmost importance to define the exact aggregation state of peptide drugs, particularly when the API is marketed as a ready-to-use solution. Consequently, appropriate non-destructive techniques able to replicate the peptide environment must be employed. In our work, we exploited Asymmetrical Flow Field-Flow Fractionation (AF4), connected to UV, dRI, fluorescence, and MALS detectors, to fully characterize the aggregation state of Liraglutide, a peptide API used for the treatment of diabetes type 2 and chronic obesity. In previous studies, Liraglutide was hypothesized to assemble into hexa-octamers in phosphate buffer, but no information on its behavior in the formulation medium was provided up to now. The method used allowed researchers to work using formulation as the mobile phase with excellent recoveries and LoQ/LoD, discerning between stable and degraded samples, and detecting, when present, aggregates up to 108 Da. The native state of Liraglutide was assessed and found to be an association into pentamers, with a non-spherical conformation. Combined to benchmark analyses, the sameness study was complete and descriptive, also giving insight on the aggregation process and covalent/non-covalent aggregate types. Full article
(This article belongs to the Special Issue Field-Flow Fractionation in Chemical Biology)
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Review

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38 pages, 5456 KiB  
Review
Field-Flow Fractionation in Molecular Biology and Biotechnology
by Stefano Giordani, Valentina Marassi, Anna Placci, Andrea Zattoni, Barbara Roda and Pierluigi Reschiglian
Molecules 2023, 28(17), 6201; https://doi.org/10.3390/molecules28176201 - 23 Aug 2023
Cited by 3 | Viewed by 2186
Abstract
Field-flow fractionation (FFF) is a family of single-phase separative techniques exploited to gently separate and characterize nano- and microsystems in suspension. These techniques cover an extremely wide dynamic range and are able to separate analytes in an interval between a few nm to [...] Read more.
Field-flow fractionation (FFF) is a family of single-phase separative techniques exploited to gently separate and characterize nano- and microsystems in suspension. These techniques cover an extremely wide dynamic range and are able to separate analytes in an interval between a few nm to 100 µm size-wise (over 15 orders of magnitude mass-wise). They are flexible in terms of mobile phase and can separate the analytes in native conditions, preserving their original structures/properties as much as possible. Molecular biology is the branch of biology that studies the molecular basis of biological activity, while biotechnology deals with the technological applications of biology. The areas where biotechnologies are required include industrial, agri-food, environmental, and pharmaceutical. Many species of biological interest belong to the operational range of FFF techniques, and their application to the analysis of such samples has steadily grown in the last 30 years. This work aims to summarize the main features, milestones, and results provided by the application of FFF in the field of molecular biology and biotechnology, with a focus on the years from 2000 to 2022. After a theoretical background overview of FFF and its methodologies, the results are reported based on the nature of the samples analyzed. Full article
(This article belongs to the Special Issue Field-Flow Fractionation in Chemical Biology)
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