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Analytical Chemistry Presents with State-of-the-Art Molecular Recognition and Interaction, 2nd Edition

A special issue of Molecules (ISSN 1420-3049). This special issue belongs to the section "Analytical Chemistry".

Deadline for manuscript submissions: 31 October 2024 | Viewed by 1138

Special Issue Editor


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Guest Editor
College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China
Interests: rubber analysis; food analysis; high performance liquid chromatography; capillary electrophoresis; mass spectrometry
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

In the 21st century, accompanied by the continuous development of industrialization, urbanization, and globalization, both analytical and bioanalytical chemistry with the molecular science of interaction and recognition play important roles and have shown obvious evolution in various fields and achieved remarkable advances in recent years, as evidenced by many state-of-the-art research practices.

In order to tackle the great challenges of food security, public health, and environment preservation, this Special Issue will include both well-drafted review and research manuscripts related but not limited to high-performance gas/liquid chromatographic technologies hyphenated with high-resolution mass spectrometry, targeted or non-targeted proteomic/metabonomic profiling, antibody- or aptamer-based rapid assay, microfluidic/nanofluidic analysis, novel molecular detection technologies, and highly sensitive analytical or bioanalytical sensors.

This Special Issue will broadly cover analytical targets that are biomarkers for medical diagnosis, pharmaceuticals for disease control, doping drugs, residual agrochemicals, natural toxins, traditional and novel environmental contaminants, pathogenic food contaminants, illegal food adulterants, food allergens, GMOs, and microbial pathogens, etc.

In addition, to better ensure the quality of analysis and risk management, analytically related research on proficiency test practices, preparations of reference materials with material certification or value assignment efforts, and risk analysis/assessment attempts for certain deleterious analytes or metabolites are also welcome.

The aim of this Special Issue is to not only present a general overview of the most advanced analytical schemes for analyzing and identifying analyte molecules or risk factors but also to outline the current research trends in various analytical domains. The Special Issue will help to acquaint the molecular science community with cutting-edge analytical principles and approaches thereof.

Prof. Dr. Yiyang Dong
Guest Editor

Manuscript Submission Information

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Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Molecules is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • ambient ionization
  • visualization
  • mass profiling
  • chromatography
  • microfluidics
  • biosensors
  • omics research
  • aptamers

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Published Papers (1 paper)

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Research

13 pages, 2333 KiB  
Article
Self-Responsive Fluorescence Aptasensor for Lactoferrin Determination in Dairy Products
by Hao Liu, Xibao Gao, Hongwei Qin, Mengmeng Yan, Chao Zhu, Linsen Li and Feng Qu
Molecules 2024, 29(13), 3013; https://doi.org/10.3390/molecules29133013 - 25 Jun 2024
Viewed by 931
Abstract
In this study, a self-responsive fluorescence aptasensor was established for the determination of lactoferrin (Lf) in dairy products. Herein, the aptamer itself functions as both a recognition element that specifically binds to Lf and a fluorescent signal reporter in conjunction with fluorescent moiety. [...] Read more.
In this study, a self-responsive fluorescence aptasensor was established for the determination of lactoferrin (Lf) in dairy products. Herein, the aptamer itself functions as both a recognition element that specifically binds to Lf and a fluorescent signal reporter in conjunction with fluorescent moiety. In the presence of Lf, the aptamer preferentially binds to Lf due to its specific and high-affinity recognition by folding into a self-assembled and three-dimensional spatial structure. Meanwhile, its reduced spatial distance in the aptamer–Lf complex induces a FRET phenomenon based on the quenching of 6-FAM by amino acids in the Lf protein, resulting in a turn-off of the fluorescence of the system. As a result, the Lf concentration can be determined straightforwardly corresponding to the change in the self-responsive fluorescence signal. Under the optimized conditions, good linearities (R2 > 0.99) were achieved in an Lf concentration range of 2~10 μg/mL for both standard solutions and the spiked matrix, as well as with the desirable detection limits of 0.68 μg/mL and 0.46 μg/mL, respectively. Moreover, the fluorescence aptasensor exhibited reliable recoveries (89.5–104.3%) in terms of detecting Lf in three commercial samples, which is comparable to the accuracy of the HPCE method. The fluorescence aptasensor offers a user-friendly, cost-efficient, and promising sensor platform for point-of-need detection. Full article
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