Novel Approaches in Mechanobiology Research

A special issue of Methods and Protocols (ISSN 2409-9279).

Deadline for manuscript submissions: closed (30 April 2019) | Viewed by 5206

Special Issue Editors


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Guest Editor
Mechanobiology Institute, Singapore City, Singapore

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Guest Editor
Institut de Biologie du Developpement de Marseille, Marseille, France

Special Issue Information

Dear Colleagues,

Mechanobiology has rapidly gained importance in the last few decades. It is a very interdisciplinary field that studies the importance of mechanical forces and environmental sensing across all traditional fields of biology (cell biology, developmental biology, immunology). It is rapidly gaining thrust in biomedical sciences. Measuring and applying forces in biological samples, understanding how the biophysical parameters of the microenvironment are sensed and integrated at the molecular, cellular and tissue levels has raised important methodological challenges.

This Special Issue aims a providing a platform for new methods in the mechanobiology field. New conceptual, imaging, microfabrication, biochemical, biomaterial, biophysical and computational approaches will be considered.

We invite the community to submit peer-reviewed methods/protocols or reviews in the above-mentioned fields to start building a repository of methods. We expect that this special issue will contribute to help mechanobiology bridging with traditional biology fields often hampered by difficulties of interdisciplinarity.

Dr. Virgile Viasnoff
Dr. Pierre-François Lenne
Guest Editors

Manuscript Submission Information

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Keywords

  • mechanobiology
  • biophysical characterization
  • environmental sensing
  • imaging
  • biomaterial
  • organoid
  • cell culture
  • mechanical force

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Published Papers (1 paper)

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Research

14 pages, 5481 KiB  
Article
A Combined AFM and Lateral Stretch Device Enables Microindentation Analyses of Living Cells at High Strains
by Dave Ahrens, Wolfgang Rubner, Ronald Springer, Nico Hampe, Jenny Gehlen, Thomas M. Magin, Bernd Hoffmann and Rudolf Merkel
Methods Protoc. 2019, 2(2), 43; https://doi.org/10.3390/mps2020043 - 24 May 2019
Cited by 12 | Viewed by 4791
Abstract
Mechanical characterization of living cells undergoing substantial external strain promises insights into material properties and functional principles of mechanically active tissues. However, due to the high strains that occur in physiological situations (up to 50%) and the complex structure of living cells, suitable [...] Read more.
Mechanical characterization of living cells undergoing substantial external strain promises insights into material properties and functional principles of mechanically active tissues. However, due to the high strains that occur in physiological situations (up to 50%) and the complex structure of living cells, suitable experimental techniques are rare. In this study, we introduce a new system composed of an atomic force microscope (AFM), a cell stretching system based on elastomeric substrates, and light microscopy. With this system, we investigated the influence of mechanical stretch on monolayers of keratinocytes. In repeated indentations at the same location on one particular cell, we found significant stiffening at 25% and 50% strain amplitude. To study the contribution of intermediate filaments, we used a mutant keratinocyte cell line devoid of all keratins. For those cells, we found a softening in comparison to the wild type, which was even more pronounced at higher strain amplitudes. Full article
(This article belongs to the Special Issue Novel Approaches in Mechanobiology Research)
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