Crop Gene Editing and Molecular Breeding

A special issue of Plants (ISSN 2223-7747). This special issue belongs to the section "Plant Genetics, Genomics and Biotechnology".

Deadline for manuscript submissions: closed (23 September 2023) | Viewed by 1773

Special Issue Editors


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Guest Editor
Key Laboratory of Biology and Genetic Improvement of Horticultural Crops, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Ministry of Agriculture, Beijing 100081, China
Interests: vegetable breeding; genetics and genomics; marker‑assisted selection; gene/QTL mapping; distant hybridization
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Guest Editor
Liaoning Key Laboratory of Genetics and Breeding for Cruciferous Vegetable Crops, College of Horticulture, Shenyang Agricultural University, Shenyang 110065, China
Interests: vegetable breeding; molecular breed; plant gene; molecular marker

Special Issue Information

Dear Colleagues,

Global climate change and unfavorable abiotic and biotic factors are limiting crop productivity. Increasing crop productivity in the face of global climate change is the most important challenge facing crop breeders. In the past, crop breeding was conducted mostly using traditional methodologies. With the rapid development of next-generation sequencing technology, crop breeding has entered the genome era. New genetic tools have been applied to germplasm resource evaluation and conservation, population structure, high-throughput screening of breeding populations, and trait marker association. The introduction of applied genetics to agriculture through plant breeding facilitated the development of varieties with improved crop productivity. Their successful application in the development of elite germplasm, with high yield, quality, and resistance to biotic and abiotic stresses, appears promising.

This Special Issue aims to provide a comprehensive overview of crop breeding efforts, focusing on all aspects of crop breeding, including but not limited to conventional and molecular breeding. Authors are invited to submit contributions of all types, including original research papers, research concepts, communications and reviews. The research of crop genetics and breeding should be accelerated to help the development of the crop industry.

Prof. Dr. Yangyong Zhang
Prof. Dr. Zhiyong Liu
Guest Editors

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Published Papers (1 paper)

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Research

14 pages, 4903 KiB  
Article
Transcriptome Analysis Reveals Key Genes and Pathways Associated with the Regulation of Flowering Time in Cabbage (Brassica oleracea L. var. capitata)
by Jiao Wang, Bin Zhang, Huiling Guo, Li Chen, Fengqing Han, Chao Yan, Limei Yang, Mu Zhuang, Honghao Lv, Yong Wang, Jialei Ji and Yangyong Zhang
Plants 2023, 12(19), 3413; https://doi.org/10.3390/plants12193413 - 28 Sep 2023
Cited by 3 | Viewed by 1411
Abstract
Flowering time is an important agronomic trait in cabbage (Brassica oleracea L. var. capitata), but the molecular regulatory mechanism underlying flowering time regulation in cabbage remains unclear. In this study, transcriptome analysis was performed using two sets of cabbage materials: (1) [...] Read more.
Flowering time is an important agronomic trait in cabbage (Brassica oleracea L. var. capitata), but the molecular regulatory mechanism underlying flowering time regulation in cabbage remains unclear. In this study, transcriptome analysis was performed using two sets of cabbage materials: (1) the early-flowering inbred line C491 (P1) and late-flowering inbred line B602 (P2), (2) the early-flowering individuals F2-B and late-flowering individuals F2-NB from the F2 population. The analysis revealed 9508 differentially expressed genes (DEGs) common to both C491_VS_ B602 and F2-B_VS_F2-NB. The Kyoto Encyclopedia of Genes and Genomes (KEGGs) analysis showed that plant hormone signal transduction and the MAPK signaling pathway were mainly enriched in up-regulated genes, and ribosome and DNA replication were mainly enriched in down-regulated genes. We identified 321 homologues of Arabidopsis flowering time genes (Ft) in cabbage. Among them, 25 DEGs (11 up-regulated and 14 down-regulated genes) were detected in the two comparison groups, and 12 gene expression patterns closely corresponded with the different flowering times in the two sets of materials. Two genes encoding MADS-box proteins, Bo1g157450 (BoSEP2-1) and Bo5g152700 (BoSEP2-2), showed significantly reduced expression in the late-flowering parent B602 compared with the early-flowering parent C491 via qRT-PCR analysis, which was consistent with the RNA-seq data. Next, the expression levels of Bo1g157450 (BoSEP2-1) and Bo5g152700 (BoSEP2-2) were analyzed in two other groups of early-flowering and late-flowering inbred lines, which showed that their expression patterns were consistent with those in the parents. Sequence analysis revealed that three and one SNPs between B602 and C491 were identified in Bo1g157450 (BoSEP2-1) and Bo5g152700 (BoSEP2-2), respectively. Therefore, BoSEP2-1 and BoSEP2-2 were designated as candidates for flowering time regulation through a potential new regulatory pathway. These results provide new insights into the molecular mechanisms underlying flowering time regulation in cabbage. Full article
(This article belongs to the Special Issue Crop Gene Editing and Molecular Breeding)
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