Post-transcriptional Regulation of Gene Expression in Plants: Both Quantity and Quality Matter

A special issue of Plants (ISSN 2223-7747). This special issue belongs to the section "Plant Genetics, Genomics and Biotechnology".

Deadline for manuscript submissions: closed (31 March 2023) | Viewed by 2674

Special Issue Editor


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Guest Editor
Laboratoire Génome et Développement des Plantes, LGDP, UMR5096, CNRS/Université de Perpignan, 66860 Perpignan, France
Interests: mRNA metabolism; mRNA decay; translation regulation; mRNA storage; post-transcriptional regulation; heat stress response
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Special Issue Information

Dear Colleagues,

Post-transcriptional regulations of messenger RNA are keystones in gene expression and appear to be highly important for proper organism development and response to environmental cues. Both the quality and quantity of mRNAs are essential to maintain homeostasis.

In this context, the main purpose of this Special Issue is to highlight recent and original findings in mRNA metabolism in plants. This Special Issue invites submission in all aspects of post-transcriptional regulation in plants, including, but not limited to, translation regulation, mRNA turnover, mRNA storage or mRNA processing. Findings in response to developmental or environmental cues are welcome. Original research papers, methods, reviews and perspectives are welcome.

Dr. Rémy Merret
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Plants is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • mRNA metabolism
  • mRNA translation
  • mRNA storage
  • mRNA modifications
  • mRNA decay
  • RNA-binding proteins
  • mRNA processing
  • Transcriptomic, Epitranscriptomic, RNA quality control

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Published Papers (1 paper)

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9 pages, 1383 KiB  
Technical Note
Monitoring mRNA Half-Life in Arabidopsis Using Droplet Digital PCR
by Alexandre Boubegtitene and Rémy Merret
Plants 2022, 11(19), 2616; https://doi.org/10.3390/plants11192616 - 5 Oct 2022
Cited by 1 | Viewed by 2270
Abstract
mRNA decay is an important process in post-transcriptional regulation; in addition, it plays a crucial role in plant development and response to stress. The development of new tools to quantify mRNA decay intermediates is thus important to better characterize the dynamic of mRNA [...] Read more.
mRNA decay is an important process in post-transcriptional regulation; in addition, it plays a crucial role in plant development and response to stress. The development of new tools to quantify mRNA decay intermediates is thus important to better characterize the dynamic of mRNA decay in various conditions. Here, we applied droplet digital PCR (ddPCR), a recent and precise PCR technology, to determine mRNA half-life in Arabidopsis seedlings. We demonstrated that ddPCR can correctly assess mRNA half-life from a wide variety of transcripts in a reproducible manner. We also demonstrated that thanks to multiplexing mRNA, the half-life of multiple transcripts can be followed in the same reaction. As ddPCR allows precise quantification, we proposed that this approach is highly suitable when a low amount of RNA is available; for the detection of many targets or for the analysis of lowly expressed transcripts. Full article
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