Plant Developmental Biology Technology and In Situ Hybridization Experiment Technology

A special issue of Plants (ISSN 2223-7747). This special issue belongs to the section "Plant Molecular Biology".

Deadline for manuscript submissions: closed (10 December 2023) | Viewed by 1889

Special Issue Editor


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Guest Editor
State Key Laboratory of Protein & Plant Gene Research, School of Life Sciences, Peking University, Beijing 100871, China
Interests: plant biotechnology

Special Issue Information

Dear Colleagues,

The development of individual plants is a complex process finely regulated by multiple genes. From embryonic development to plant aging and death, each step includes many developmental events. Using the theoretical basis and advanced biological technology of other disciplines in biologicals, assessing the plant development process at the molecular level will help humans to understand and reveal the mechanism of each developmental event in the plant kingdom more deeply, thus creating broader applications for production practices. In situ hybridization can be used to reveal the location of a specific nucleic acid sequence with labeled antisense RNA probes in a chromosome or tissue, which is a critical step in understanding the organization, regulation, and function of genes.

This research topic is dedicated to the most recent advances in plant developmental biology, both from a practical and a fundamental perspective. Original research, review, and mini-review manuscripts are welcome that are on topics including: plant embryogenesis and seed formation; plant cells expressing totipotency to regenerate plants in the most common way and introduce the basic content of plant development after embryogenesis, including the development of the shoot apical meristem and its lateral organs; root (including lateral root and root hair) development and regulation; the sex determination of plants; gametogenesis and gametophyte development; flower development and regulation; and fruit development and regulation. Papers on the biotechnological applications of plant developmental biology, such as cloning, hap, and plant genetic transformation and cryopreservation, among others, are welcome. Manuscripts dealing with the more recent advances in the fundamental research on plant embryogenesis, such as functional genomics, transcriptomics, and proteomics analysis, are also encouraged to be submitted, as are those concerning the role of epigenetics on embryo formation and development.

Dr. Donghui Wang
Guest Editor

Manuscript Submission Information

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Keywords

  • cloning
  • embryo development
  • plant embryogenesis
  • totipotency
  • plant developmental biology

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Published Papers (1 paper)

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Review

14 pages, 1153 KiB  
Review
Aggregation-Induced Emission Luminogens: A New Possibility for Efficient Visualization of RNA in Plants
by Zheng-Chao Yang, Li-Xiang Zhao, Yu-Qi Sang, Xin Huang, Xuan-Chen Lin and Zhi-Ming Yu
Plants 2024, 13(5), 743; https://doi.org/10.3390/plants13050743 - 6 Mar 2024
Viewed by 1474
Abstract
RNAs play important roles in regulating biological growth and development. Advancements in RNA-imaging techniques are expanding our understanding of their function. Several common RNA-labeling methods in plants have pros and cons. Simultaneously, plants’ spontaneously fluorescent substances interfere with the effectiveness of RNA bioimaging. [...] Read more.
RNAs play important roles in regulating biological growth and development. Advancements in RNA-imaging techniques are expanding our understanding of their function. Several common RNA-labeling methods in plants have pros and cons. Simultaneously, plants’ spontaneously fluorescent substances interfere with the effectiveness of RNA bioimaging. New technologies need to be introduced into plant RNA luminescence. Aggregation-induced emission luminogens (AIEgens), due to their luminescent properties, tunable molecular size, high fluorescence intensity, good photostability, and low cell toxicity, have been widely applied in the animal and medical fields. The application of this technology in plants is still at an early stage. The development of AIEgens provides more options for RNA labeling. Click chemistry provides ideas for modifying AIEgens into RNA molecules. The CRISPR/Cas13a-mediated targeting system provides a guarantee of precise RNA modification. The liquid–liquid phase separation in plant cells creates conditions for the enrichment and luminescence of AIEgens. The only thing that needs to be looked for is a specific enzyme that uses AIEgens as a substrate and modifies AIEgens onto target RNA via a click chemical reaction. With the development and progress of artificial intelligence and synthetic biology, it may soon be possible to artificially synthesize or discover such an enzyme. Full article
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