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Application of Proteomics Technology in Plant Stress Biology
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Application of Proteomics Technology in Plant Stress Biology

A special issue of Plants (ISSN 2223-7747). This special issue belongs to the section "Plant Molecular Biology".

Deadline for manuscript submissions: closed (31 August 2023) | Viewed by 6670

Special Issue Editors

Dr. Candida Vannini

E-Mail Website
Guest Editor
Biotechnology and Life Science Department, University of Insubria, via Dunant 3, 21100 Varese, Italy
Interests: plant stress response; proteomics; post-translational modifications
Dr. Guido Domingo

E-Mail Website
Guest Editor
Biotechnology and Life Science Department, University of Insubria, via Dunant 3, 21100 Varese, Italy
Interests: plant stress response; proteomics; post-translational modifications

Special Issue Information

Dear Colleagues,

In recent years, technological advances have seen the development of the omics sciences in order to understand the molecular mechanisms underlying plants responses to stress. Among them, proteomics is a dynamic and powerful tool to study protein changes that occur in plants exposed to stress. In fact, plant response and acclimation to stress are associated with profound changes in proteome composition and both biotic and abiotic stresses have a strong impact on plant proteome leading to alterations in protein relative abundance, cellular localization, and protein interactions. Moreover, the emerging picture defines the post-translational mechanisms, such as phosphorylation, glycosylation, and ubiquitination, as regulatory systems related to the plant stress response that can rapidly change the functional diversity of the proteome.

Studying changes that occur at the proteomic level could help understand the pathways involved in stress tolerance and/or identifying key proteins that can be used for the genetic improvement of plants.

As guest editors of the Special Issue "Application of Proteomics Technologies in Plant Stress Biology" of Plants, we would like to invite you to contribute with original research articles and reviews addressing the application of proteomics technology in elucidating plants response to both biotic and abiotic stress. The deadline for manuscript submission is 20 February 2023.

Dr. Candida Vannini
Dr. Guido Domingo
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Plants is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • proteomics
  • plant stress
  • stress tolerance
  • post-translational modifications

Published Papers (4 papers)

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Research

16 pages, 2070 KiB  
Article
Site-Directed Mutations at Phosphorylation Sites in Zea mays PHO1 Reveal Modulation of Enzymatic Activity by Phosphorylation at S566 in the L80 Region
by Noman Shoaib, Nishbah Mughal, Lun Liu, Ali Raza, Leiyang Shen and Guowu Yu
Plants 2023, 12(18), 3205; https://doi.org/10.3390/plants12183205 - 8 Sep 2023
Cited by 1 | Viewed by 995
Abstract
Starch phosphorylase (PHO) is a pivotal enzyme within the GT35-glycogen–phosphorylase (GT; glycosyltransferases) superfamily. Despite the ongoing debate surrounding the precise role of PHO1, evidence points to its substantial influence on starch biosynthesis, supported by its gene expression profile and subcellular localization. Key to [...] Read more.
Starch phosphorylase (PHO) is a pivotal enzyme within the GT35-glycogen–phosphorylase (GT; glycosyltransferases) superfamily. Despite the ongoing debate surrounding the precise role of PHO1, evidence points to its substantial influence on starch biosynthesis, supported by its gene expression profile and subcellular localization. Key to PHO1 function is the enzymatic regulation via phosphorylation; a myriad of such modification sites has been unveiled in model crops. However, the functional implications of these sites remain to be elucidated. In this study, we utilized site-directed mutagenesis on the phosphorylation sites of Zea mays PHO1, replacing serine residues with alanine, glutamic acid, and aspartic acid, to discern the effects of phosphorylation. Our findings indicate that phosphorylation exerts no impact on the stability or localization of PHO1. Nonetheless, our enzymatic assays unveiled a crucial role for phosphorylation at the S566 residue within the L80 region of the PHO1 structure, suggesting a potential modulation or enhancement of PHO1 activity. These data advance our understanding of starch biosynthesis regulation and present potential targets for crop yield optimization. Full article
(This article belongs to the Special Issue Application of Proteomics Technology in Plant Stress Biology)
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17 pages, 2674 KiB  
Article
Role of NPR1 in Systemic Acquired Stomatal Immunity
by Qijie Guan, Lisa David, Riley Moran, Ivan Grela, Angelica Ortega, Peter Scott, Lindsey Warnock and Sixue Chen
Plants 2023, 12(11), 2137; https://doi.org/10.3390/plants12112137 - 29 May 2023
Cited by 3 | Viewed by 1699
Abstract
Stomatal immunity is the primary gate of the plant pathogen defense system. Non-expressor of Pathogenesis Related 1 (NPR1) is the salicylic acid (SA) receptor, which is critical for stomatal defense. SA induces stomatal closure, but the specific role of NPR1 in guard cells [...] Read more.
Stomatal immunity is the primary gate of the plant pathogen defense system. Non-expressor of Pathogenesis Related 1 (NPR1) is the salicylic acid (SA) receptor, which is critical for stomatal defense. SA induces stomatal closure, but the specific role of NPR1 in guard cells and its contribution to systemic acquired resistance (SAR) remain largely unknown. In this study, we compared the response to pathogen attack in wild-type Arabidopsis and the npr1-1 knockout mutant in terms of stomatal movement and proteomic changes. We found that NPR1 does not regulate stomatal density, but the npr1-1 mutant failed to close stomata when under pathogen attack, resulting in more pathogens entering the leaves. Moreover, the ROS levels in the npr1-1 mutant were higher than in the wild type, and several proteins involved in carbon fixation, oxidative phosphorylation, glycolysis, and glutathione metabolism were differentially changed in abundance. Our findings suggest that mobile SAR signals alter stomatal immune response possibly by initiating ROS burst, and the npr1-1 mutant has an alternative priming effect through translational regulation. Full article
(This article belongs to the Special Issue Application of Proteomics Technology in Plant Stress Biology)
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20 pages, 2327 KiB  
Article
Phosphoprotein Profile of Rice (Oryza sativa L.) Seedlings under Osmotic Stress after Pretreatment with Chitosan
by Wasinee Pongprayoon, Atikorn Panya, Janthima Jaresitthikunchai, Narumon Phaonakrop and Sittiruk Roytrakul
Plants 2022, 11(20), 2729; https://doi.org/10.3390/plants11202729 - 15 Oct 2022
Cited by 3 | Viewed by 1609
Abstract
This study aims to identify novel chitosan (CTS)-responsive phosphoproteins in Leung Pratew 123 (LPT123) and Khao Dawk Mali 105 (KDML105) as drought-sensitive rice cultivars and differences in the CTS response. Rice seeds were soaked in CTS solution before germination, and 2- and 4-week-old [...] Read more.
This study aims to identify novel chitosan (CTS)-responsive phosphoproteins in Leung Pratew 123 (LPT123) and Khao Dawk Mali 105 (KDML105) as drought-sensitive rice cultivars and differences in the CTS response. Rice seeds were soaked in CTS solution before germination, and 2- and 4-week-old rice seedlings sprayed with CTS before osmotic stress comprised the following four groups: (1) seedlings treated with distilled water; (2) seedlings treated with CTS; (3) seedlings pretreated with distilled water and subjected to osmotic stress; and (4) seedlings pretreated with CTS and subjected to osmotic stress. Phosphoproteins of leaf tissues were enriched using immobilized metal affinity chromatography (IMAC) before tryptic digestion and analysis via LC-MS. Phosphoprotein profiling analyses led to the identification of 4721 phosphoproteins representing 1052 and 1040 unique phosphoproteins in the LPT123 and KDML105 seedlings, respectively. In response to CTS pretreatment before osmotic stress, 22 differently expressed proteins were discovered, of which 10 and 12 were identified in the LPT123 and KDML105, respectively. These proteins are typically involved in signaling, transport, protein folding, protein degradation, and metabolism. This study provides fruitful data to understand the signal transduction mechanisms of rice seedlings pretreated with CTS before exposure to osmotic stress. Full article
(This article belongs to the Special Issue Application of Proteomics Technology in Plant Stress Biology)
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18 pages, 4023 KiB  
Article
Quantitative Proteomics and Functional Characterization Reveal That Glutathione Peroxidases Act as Important Antioxidant Regulators in Mulberry Response to Drought Stress
by Minjuan Zhang, Wenqiang Li, Shuaijun Li, Junru Gao, Tiantian Gan, Qinying Li, Lijun Bao, Feng Jiao, Chao Su and Yonghua Qian
Plants 2022, 11(18), 2350; https://doi.org/10.3390/plants11182350 - 8 Sep 2022
Cited by 5 | Viewed by 1771
Abstract
Mulberry (Morus alba L.) has been an economically important food crop for the domesticated silkworm, Bombyx mori, in China for more than 5000 years. However, little is known about the mechanism underlying mulberry response to environmental stress. In this study, quantitative [...] Read more.
Mulberry (Morus alba L.) has been an economically important food crop for the domesticated silkworm, Bombyx mori, in China for more than 5000 years. However, little is known about the mechanism underlying mulberry response to environmental stress. In this study, quantitative proteomics was applied to elucidate the molecular mechanism of drought response in mulberry. A total of 604 differentially expressed proteins (DEPs) were identified via LC-MS/MS. The proteomic profiles associated with antioxidant enzymes, especially five glutathione peroxidase (GPX) isoforms, as a scavenger of reactive oxygen species (ROS), were systematically increased in the drought-stressed mulberry. This was further confirmed by gene expression and enzymatic activity. Furthermore, overexpression of the GPX isoforms led to enhancements in both antioxidant system and ROS-scavenging capacity, and greater tolerance to drought stress in transgenic plants. Taken together, these results indicated that GPX-based antioxidant enzymes play an important role in modulating mulberry response to drought stress, and higher levels of GPX can improve drought tolerance through enhancing the capacity of the antioxidant system for ROS scavenging. Full article
(This article belongs to the Special Issue Application of Proteomics Technology in Plant Stress Biology)
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