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Processes
Special Issues
Applications of Chromatographic Separation Techniques in Food and Chemistry—Second Edition
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Applications of Chromatographic Separation Techniques in Food and Chemistry—Second Edition

A special issue of Processes (ISSN 2227-9717). This special issue belongs to the section "Separation Processes".

Deadline for manuscript submissions: 20 August 2024 | Viewed by 1494

Special Issue Editor

Prof. Dr. Alina Pyka-Pająk

E-Mail Website
Guest Editor
Department of Analytical Chemistry, Faculty of Pharmaceutical Sciences in Sosnowiec, Medical University of Silesia in Katowice, Jagiellońska 4, 41-200 Sosnowiec, Poland
Interests: liquid chromatography; spectrophotometry; densitometry; organic compounds analysis; QSAR; QSRR; QSPR; pharmaceutical analysis; purity of pharmaceutical preparations
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

This is the second volume of the successful Special Issue we launched, titled "Applications of Chromatographic Separation Techniques in Food and Chemistry". In the first volume, we published 23 articles. (https://www.mdpi.com/journal/processes/special_issues/Chromatographic_Separation_Techniques).

Chromatographic techniques are by far the most powerful and versatile methods available to the modern analyst. Because of their good separation capacity, various chromatographic methods combined with modern detection systems are able to quickly separate very complicated mixtures into their individual components in a single-step process and are also capable of the simultaneous quantitative determination of each constituent.

Currently, chromatography is an extremely versatile technique which allows for the rapid separation of gases and volatile substances using gas chromatography, involatile chemicals, and materials of extremely high molecular weight using liquid chromatography, and, if necessary, very inexpensively using thin-layer chromatography coupled with densitometry. Therefore, chromatographic analyses are a widespread research method used in virtually all industries, also involving food processing as well as chemical synthesis. They allow for the detection of even trace amounts of substances in the finished product, which in turn gives the possibility of assessing the meticulous quality control of food products or the purity of newly synthesized bioactive molecules as potential new drug candidates, as well as others.

This Special Issue, focused on “Applications of Chromatographic Separation Techniques in Food and Chemistry”, aims to curate novel advances in the development and application of chromatographic separation techniques in food and chemical analyses. Topics of interest for this Special Issue include, but are not limited to, the following:

  • Chromatographic techniques used in ascertaining food quality and authenticity;
  • Chromatographic analysis of food additives, including food preservatives, antioxidants, sweeteners, colors, etc.;
  • Basic research and applications of chromatographic techniques in chemistry;
  • Application of chromatographic techniques in pharmaceutical analysis;
  • The development of new chromatographic methods in the analysis of food and chemical compounds, including bioactive molecules;
  • Chromatography as a separation technique useful in the chemical process industry; for analysis, isolation, and purification of various chemical compounds as the components of small- and large-scale production.

Original research papers as well as reviews related to the abovementioned areas are most welcome.

Prof. Dr. Alina Pyka-Pająk
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Processes is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2400 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • chromatographic techniques (GC, HPLC, TLC, TLC-densitometry)
  • food components
  • food quality
  • chemical analysis
  • analysis of bioactive compounds
  • pharmaceutical analysis
  • separation techniques

Published Papers (2 papers)

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Research

15 pages, 3133 KiB  
Article
Development and Validation of Ultra-Performance Liquid Chromatography (UPLC) Method for Simultaneous Quantification of Hydrochlorothiazide, Amlodipine Besylate, and Valsartan in Marketed Fixed-Dose Combination Tablet
by Doaa Hasan Alshora, Abdelrahman Y. Sherif and Mohamed Abbas Ibrahim
Processes 2024, 12(6), 1259; https://doi.org/10.3390/pr12061259 - 19 Jun 2024
Viewed by 411
Abstract
Fixed-dose combination therapy is considered a practical approach in the treatment of various diseases, as it can simultaneously target different mechanisms of action that achieve the required therapeutic efficacy through a synergistic effect. A combination of hydrochlorothiazide (HTZ), amlodipine (AMD), and valsartan (VLS) [...] Read more.
Fixed-dose combination therapy is considered a practical approach in the treatment of various diseases, as it can simultaneously target different mechanisms of action that achieve the required therapeutic efficacy through a synergistic effect. A combination of hydrochlorothiazide (HTZ), amlodipine (AMD), and valsartan (VLS) has been created for the treatment of hypertension. Therefore, the aim of this study was to develop an optimized UPLC method for the simultaneous quantification of this combination. A DoE at a level of 32 was used to investigate the effects of column temperature (20, 30, and 40 °C) and formic acid concentration (0.05, 0.15, and 0.25%) on the retention time of each active pharmaceutical ingredient (API), the peak area, and the peak symmetry, as well as the resolution between HTZ-AMD and AMD-VLS peaks. The optimized analytical method was validated and used to extract the three APIs from the marketed product. The optimized analytical condition with a column temperature of 27.86 °C and a formic acid concentration of 0.172% showed good separation of the three APIs in 1.62 ± 0.006, 3.59 ± 0.002, and 3.94 ± 0.002 min for HTZ, AMD, and VST, respectively. The developed method was linear with the LOQ for a HTC, AMD, and VST of 0.028, 0.038, and 0.101 ppm, respectively. Moreover, the developed assay was sustainable and robust, with an RSD % of less than 2%. The application of this method in the extraction of HTZ, AMD, and VST from the Exforge® marketed product showed good separation with a measurable drug content of 23.5 ± 0.7, 9.68 ± 0.1, and 165.2 ± 5.2 mg compared to the label claims of 25/10/160 for HTZ, AMD, and VST, respectively. Full article
(This article belongs to the Special Issue Applications of Chromatographic Separation Techniques in Food and Chemistry—Second Edition)
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15 pages, 5125 KiB  
Article
Comparison of the Limit of Detection of Paracetamol, Propyphenazone, and Caffeine Analyzed Using Thin-Layer Chromatography and High-Performance Thin-Layer Chromatography
by Katarzyna Bober-Majnusz and Alina Pyka-Pająk
Processes 2024, 12(6), 1153; https://doi.org/10.3390/pr12061153 - 3 Jun 2024
Viewed by 323
Abstract
TLC (thin-layer chromatography) and HPTLC (high-performance thin-layer chromatography) in normal (NP) and reversed (RP) phase systems were combined with densitometry to analyze caffeine, propyphenazone, and paracetamol. This work aims to check whether comparable limit of detection (LOD) values can be obtained on TLC [...] Read more.
TLC (thin-layer chromatography) and HPTLC (high-performance thin-layer chromatography) in normal (NP) and reversed (RP) phase systems were combined with densitometry to analyze caffeine, propyphenazone, and paracetamol. This work aims to check whether comparable limit of detection (LOD) values can be obtained on TLC and HPTLC plates. Analyses were performed on five (NP) or four (RP) different stationary phases (chromatographic plates), testing, in both cases, three mobile phases. It is shown that by using both TLC and HPTLC plates, it is possible to develop chromatographic conditions that enable the detection of compounds analyzed in amounts ranging from a dozen to several dozen µg/spot. In the RP system, lower LOD values for all tested compounds were obtained using TLC than HPTLC. However, performing analyses in the NP, similar (of the same order) LOD values were obtained for caffeine, propyphenazone, and paracetamol when using both TLC and HPTLC plates. For example, during the NP-HPTLC analysis using silica gel 60F254 plates (#1.05548) and mobile phase B (n-hexane—acetone—ammonia, 25:25:0.5, v/v/v), LOD values for caffeine, propyphenazone, and paracetamol were 0.010, 0.046, and 0.030 μg/spot, respectively. During NP-TLC analysis using silica gel 60F254 (#1.05554 plates) and the mobile phase C (chloroform—toluene—ethyl acetate—methanol—80% acetic acid, 18:18:7.5:6:0.3, v/v), the values of LOD were 0.054, 0.029, and 0.016 μg/spot, respectively. During RP-TLC analysis using TLC RP-18F254 plates (#1.05559) and mobile phase F (methanol-water, 40:10, v/v), the LOD values were 0.019, 0.024, and 0.053 μg/spot, respectively. Therefore, for economical reasons, TLC plates should be recommended for analyses of caffeine, propyphenazone, and paracetamol, which are several times cheaper than HPTLC plates. Full article
(This article belongs to the Special Issue Applications of Chromatographic Separation Techniques in Food and Chemistry—Second Edition)
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