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Processes
Special Issues
New Frontiers in Chromatographic Separation Technology
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New Frontiers in Chromatographic Separation Technology

A special issue of Processes (ISSN 2227-9717). This special issue belongs to the section "Separation Processes".

Deadline for manuscript submissions: closed (15 December 2024) | Viewed by 6568

Special Issue Editors

Prof. Dr. Laurence Muhr

E-Mail Website
Guest Editor
Laboratoire Réactions et Génie des Procédés, Université de Lorraine, CNRS, 54000 Nancy, France
Interests: separation processes; preparative chromatography; ion exchange; electrodeionization; hydrometallurgy
Dr. Laetitia Canabady-Rochelle

E-Mail Website
Guest Editor
Laboratoire Réactions et Génie des Procédés, Université de Lorraine, CNRS, 54000 Nancy, France
Interests: screening; separation; immobilized metal–ion affinity chromatography; process simulation; metal chelation; peptides; protein; bioactivity; antioxidants

Special Issue Information

Dear Colleagues,

Separation and purification are major steps in most industrial processes. The development of new products often requires the design and establishment of new separation processes. In the case of life science industries, for example, the evolution of the pharmaceutical industry towards biopharmaceuticals has led to many innovations in chromatographic separation technologies. Similar challenges have arisen with the need to recover critical metals. Recycling or extraction of these metals from inorganic chemistry by-products previously considered as waste allows the purification and concentration of highly diluted target species in complex matrices. To meet these challenges, innovations in chromatography processes can be implemented at different scales, i.e., at the stationary phase scale, at the column scale, and at the scale of process architecture. The development of new, more selective phases, which allow for better ageing, in a material process approach, is essential. As far as the column is concerned, modeling and instrumentation development leads to a better understanding of the limiting steps and a better control of the process. In addition, many bottlenecks can be overcome through multi-column, continuous, or sequential process architectures or through hybrid processes.

This Special Issue entitled "New Frontiers in Chromatographic Separation Technology" aims to give an overview of the recent developments in chromatographic techniques to meet new industrial challenges. Progress in liquid chromatography will be more specifically addressed.

Topics of interest include, but are not limited to, the following:

  • New stationary phases for life sciences
  • Specific functional groups grafted on resins for metal ion/species separation
  • Modeling and instrumentation of columns
  • Specific column technologies (fluidized bed, etc.)
  • Multicolumn processes (continuous or sequential)
  • Hybrid ion exchange processes (electrodeionization, electro-regeneration, etc.)

Original research papers as well as reviews are welcome.

Prof. Dr. Laurence Muhr
Dr. Laetitia Canabady-Rochelle
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Processes is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2400 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • chromatography process development
  • multicolumn chromatography
  • selective stationary phase
  • electrodeionization
  • expanded bed chromatography
  • chromatography for downstream process
  • ion exchange in hydrometallurgy
  • chromatography column modeling
  • chromatography column design and control

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Further information on MDPI's Special Issue polices can be found here.

Published Papers (3 papers)

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Research

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20 pages, 1371 KiB  
Article
A New Methodology for Synthetic Peptides Purification and Counterion Exchange in One Step Using Solid-Phase Extraction Chromatography
by Amalia Giselle López-Sánchez, Karla Geraldine Rodríguez-Mejía, Kelin Johana Cuero-Amu, Natalia Ardila-Chantré, Juan Esteban Reyes-Calderón, Nicolás Mateo González-López, Kevin Andrey Huertas-Ortiz, Ricardo Fierro-Medina, Zuly Jenny Rivera-Monroy and Javier Eduardo García-Castañeda
Processes 2025, 13(1), 27; https://doi.org/10.3390/pr13010027 - 26 Dec 2024
Viewed by 338
Abstract
Synthetic peptides are commonly obtained by means of solid-phase peptide synthesis (SPPS), in which separation of the peptide from the solid support requires treatment with 92.5% v/v trifluoroacetic acid (TFA); therefore, peptides are obtained as trifluoroacetate salts. For promising anticancer/antibacterial peptides [...] Read more.
Synthetic peptides are commonly obtained by means of solid-phase peptide synthesis (SPPS), in which separation of the peptide from the solid support requires treatment with 92.5% v/v trifluoroacetic acid (TFA); therefore, peptides are obtained as trifluoroacetate salts. For promising anticancer/antibacterial peptides it is essential to exchange the counterion from trifluoroacetate to hydrochloride or acetate, since the former are more widely studied in biological activity assays. In this research, RP-SPE-based methodologies were designed, developed, and implemented for simultaneous counterion exchange and peptide purification. Critical process steps were identified and parameters such as mobile phase composition, elution, and program were optimized. Analysis of the counterion exchange reaction and characterization of the final products was performed using high-performance liquid chromatography, attenuated total reflectance, nuclear magnetic resonance, and mass spectrometry. Peptides with purities between 82–97% and a trifluoroacetate ion content less than 0.36% were obtained. This novel counterion exchange proved efficient for peptides with different characteristics such as length, polarity, polyvalency, and presence of non-natural amino acids or non-protein molecules, therefore showing a wide range of applications in the field of therapeutic peptides. The methods developed are fast, efficient, low-cost, and do not require robust instrumentation and can be routinely implemented in SPPS. Full article
(This article belongs to the Special Issue New Frontiers in Chromatographic Separation Technology)
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19 pages, 2439 KiB  
Article
Simulation of Ni2+ Chelating Peptides Separation in IMAC: Prediction of Langmuir Isotherm Parameters from SPR Affinity Data
by Rachel Irankunda, Pauline Jambon, Alexandra Marc, Jairo Andrés Camaño Echavarría, Laurence Muhr and Laetitia Canabady-Rochelle
Processes 2024, 12(3), 592; https://doi.org/10.3390/pr12030592 - 15 Mar 2024
Viewed by 963
Abstract
Chromatography modeling for simulation is a tool that can help to predict the separation of molecules inside the column. Knowledge of sorption isotherms in chromatography modeling is a crucial step and methods such as frontal analysis or batch are used to obtain sorption [...] Read more.
Chromatography modeling for simulation is a tool that can help to predict the separation of molecules inside the column. Knowledge of sorption isotherms in chromatography modeling is a crucial step and methods such as frontal analysis or batch are used to obtain sorption isotherm parameters, but they require a significant quantity of samples. This study aims to predict Langmuir isotherm parameters from Surface Plasmon Resonance (SPR) affinity data (requiring less quantity of sample) to simulate metal chelating peptides (MCPs) separation in Immobilized Metal ion Affinity Chromatography (IMAC), thanks to the analogy between both techniques. The validity of simulation was evaluated by comparing the peptide’s simulated retention time with its experimental retention time obtained by IMAC. Results showed that the peptide affinity constant (KA) can be conserved between SPR and IMAC. However, the maximal capacity (qmax) must be adjusted by a correction factor to overcome the geometry differences between IMAC (spherical particles) and SPR (plane sensor ship). Therefore, three approaches were studied; the best one was to use qmax,IMAC imidazole determined experimentally while a correction factor was applied on qmax,SPR to obtain the qmax,IMAC of the peptide, thus minimizing the discrepancy between the experimental and simulated retention times of a peptide. Full article
(This article belongs to the Special Issue New Frontiers in Chromatographic Separation Technology)
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Review

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23 pages, 2979 KiB  
Review
Separation and Analytical Techniques Used in Snake Venomics: A Review Article
by Christina Sahyoun, Mohamad Rima, César Mattei, Jean-Marc Sabatier, Ziad Fajloun and Christian Legros
Processes 2022, 10(7), 1380; https://doi.org/10.3390/pr10071380 - 14 Jul 2022
Cited by 6 | Viewed by 4266
Abstract
The deleterious consequences of snake envenomation are due to the extreme protein complexity of snake venoms. Therefore, the identification of their components is crucial for understanding the clinical manifestations of envenomation pathophysiology and for the development of effective antivenoms. In addition, snake venoms [...] Read more.
The deleterious consequences of snake envenomation are due to the extreme protein complexity of snake venoms. Therefore, the identification of their components is crucial for understanding the clinical manifestations of envenomation pathophysiology and for the development of effective antivenoms. In addition, snake venoms are considered as libraries of bioactive molecules that can be used to develop innovative drugs. Numerous separation and analytical techniques are combined to study snake venom composition including chromatographic techniques such as size exclusion and RP-HPLC and electrophoretic techniques. Herein, we present in detail these existing techniques and their applications in snake venom research. In the first part, we discuss the different possible technical combinations that could be used to isolate and purify SV proteins using what is known as bioassay-guided fractionation. In the second part, we describe four different proteomic strategies that could be applied for venomics studies to evaluate whole venom composition, including the mostly used technique: RP-HPLC. Eventually, we show that to date, there is no standard technique used for the separation of all snake venoms. Thus, different combinations might be developed, taking into consideration the main objective of the study, the available resources, and the properties of the target molecules to be isolated. Full article
(This article belongs to the Special Issue New Frontiers in Chromatographic Separation Technology)
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