Spectroscopy and Chromatography Coupled to Mass Spectrometry in Cannabinoid Research

A special issue of Separations (ISSN 2297-8739).

Deadline for manuscript submissions: closed (30 November 2020) | Viewed by 5901

Special Issue Editor


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Guest Editor
Analytical Research & Quality Control Department, Phytoplant Research SL, Spain
Interests: Cannabis sativa L.; cannabinoids; mass spectrometry; spectroscopy

Special Issue Information

Dear Colleagues,

Although, in recent decades, the cultivation of hemp has been introduced or reintroduced in several countries to obtain fiber or grains, recently, the use of cannabis, especially its active ingredients, cannabinoids, is increasingly being explored for medical and therapeutical applications.

Considering the growing interest in the study of Cannabis sativa L. for the scientific community in fields such as the pharmacological, biomedical, forensic, or agronomical (to name just a few), there is an emerging need to solve analytical problems and to provide useful knowledge and tools for such diverse areas of interest, both from the point of view of basic and technical research.

This Special Issue will bring together international experts, inviting them to provide a deep overview and analysis of scientifically sound evidence and analytical insight into the cannabis sector.

All types of articles reporting basic, translational, experimental, or clinical research that include the most recent and relevant findings in the field of spectroscopy or chromatography coupled to mass spectrometry applied to cannabinoids are welcome.

Dr. Carlos Ferreiro Vera
Guest Editor

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Keywords

  • Cannabis sativa L.
  • phytocannabinoids
  • endocannabinoids
  • synthetic cannabinoids
  • spectroscopy
  • mass spectrometry
  • liquid chromatography
  • gas chromatography

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Published Papers (1 paper)

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16 pages, 2833 KiB  
Article
Biological Activity of Cannabis sativa L. Extracts Critically Depends on Solvent Polarity and Decarboxylation
by Guillermo Moreno-Sanz, Carlos Ferreiro Vera, Carolina Sánchez-Carnerero, Xavier Nadal Roura and Verónica Sánchez de Medina Baena
Separations 2020, 7(4), 56; https://doi.org/10.3390/separations7040056 - 20 Oct 2020
Cited by 12 | Viewed by 7697
Abstract
Minor cannabinoid and non-cannabinoid molecules have been proposed to significantly contribute to the pharmacological profile of cannabis extracts. Phytoplant Research has developed highly productive cannabis cultivars with defined chemotypes, as well as proprietary methods for the extraction and purification of cannabinoids. Here, we [...] Read more.
Minor cannabinoid and non-cannabinoid molecules have been proposed to significantly contribute to the pharmacological profile of cannabis extracts. Phytoplant Research has developed highly productive cannabis cultivars with defined chemotypes, as well as proprietary methods for the extraction and purification of cannabinoids. Here, we investigate the effect of solvent selection and decarboxylation on the composition and pharmacological activity of cannabis extracts. A library of forty cannabis extracts was generated from ten different cannabis cultivars registered by Phytoplant Research at the EU Community Plant Variety Office. Plant material was extracted using two different solvents, ethanol and hexane, and crude extracts were subsequently decarboxylated or not. Cannabinoid content in the resulting extracts was quantified, and biological activity was screened in vitro at three molecular targets involved in hypoxia and inflammation (NF-κB, HIF-1α and STAT3). Changes in transcriptional activation were strongly associated to solvent selection and decarboxylation. Two decarboxylated extracts prepared with hexane were the most potent at inhibiting NF-κB transcription, while HIF-1α activation was preferentially inhibited by ethanolic extracts, and decarboxylated extracts were generally more potent at inhibiting STAT3 induction. Our results indicate that solvent selection and proper decarboxylation represent key aspects of the standardized production of cannabis extracts with reproducible pharmacological activity. Full article
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