Advances in Animal Reproductive Physiology, Reproductive Endocrine and Obstetric Diseases—2nd Edition

A special issue of Veterinary Sciences (ISSN 2306-7381). This special issue belongs to the section "Veterinary Reproduction and Obstetrics".

Deadline for manuscript submissions: 31 January 2025 | Viewed by 3834

Special Issue Editors

College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China
Interests: semen preservation; seminal plasma antioxidants; sperm proteins
Special Issues, Collections and Topics in MDPI journals
College of Veterinary Medicine, Northwest Agriculture and Forestry University, Xianyang 712100, China
Interests: embryo implantation; gestation; hormones; steroids; infertility; mastitis; endometritis; reproductive immunology
Special Issues, Collections and Topics in MDPI journals

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Guest Editor
College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China
Interests: reproductive biology; oocyte meiosis; preimplantation embryo development

Special Issue Information

Dear Colleagues,

Animal reproduction and health are hot topics in animal research. Animal reproduction is not only the basis of biodiversity but also an important factor affecting breeding and the development of the agricultural industry. In addition, with the rapid development of modern animal husbandry, greater demands have arisen in terms of the quantity and quality of dairy animals and animals for meat. In this Special Issue, we intend to cover the latest findings regarding animal reproduction and to provide novel knowledge in this research area.

Topics of interest include, but are not limited to

  • Reproductive hormones;
  • Spermatogenesis;
  • Oogenesis and follicular development;
  • Embryo development and implantation;
  • Reproductive immunology;
  • Obstetric diseases, such as mastitis and endometritis.

We look forward to receiving your contributions.

Dr. Junwei Li
Dr. Dong Zhou
Prof. Dr. Shiqiang Ju
Guest Editors

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Keywords

  • spermatogenesis
  • oogenesis
  • embryo development
  • follicular development
  • embryo implantation
  • estrus cycle
  • gestation
  • parturition
  • reproductive hormone
  • infertility
  • abortion
  • dystocia
  • endometritis
  • retained fetal membranes
  • mastitis

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Related Special Issue

Published Papers (4 papers)

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Research

9 pages, 519 KiB  
Article
Recipients’ and Environmental Factors Affecting the Pregnancy Rates of a Large, Fresh In Vitro Fertilization-Embryo Transfer Program for Dairy Cows in a Commercial Herd in China
by Chengyun Xie, Cong Huang, Longgang Yan, Ruiqi Yao, Jinbang Xiao, Mingmao Yang, Huatao Chen, Keqiong Tang, Dong Zhou, Pengfei Lin, Aihua Wang and Yaping Jin
Vet. Sci. 2024, 11(9), 410; https://doi.org/10.3390/vetsci11090410 - 5 Sep 2024
Viewed by 565
Abstract
The main objective of this study was to determine the influence of the recipient dairy cows’ breed, lactation number, estrus condition, the type, location and volume of the corpus luteum (CL) and the time of year that the embryo transfer (ET) was performed [...] Read more.
The main objective of this study was to determine the influence of the recipient dairy cows’ breed, lactation number, estrus condition, the type, location and volume of the corpus luteum (CL) and the time of year that the embryo transfer (ET) was performed on the pregnancy rates of a large, fresh in vitro fertilization–embryo transfer program for dairy cows in a commercial herd in China. The recipients were from a herd of dairy cows in Ningxia, a province in northwest China, and we statistically analyzed the data of 495 cows from 2021 to 2023. Cumulus oocyte complexes (COCS) were isolated from follicular fluid obtained through ovum pick-up (OPU) and oocytes were incubated 20–22 h for in vitro maturation (IVM). Embryos were obtained after 10–12 h of in vitro fertilization (IVF) and six days of in vitro culture (IVC). Embryos at the morula or blastocyst stage were transferred to randomly chosen recipients (n = 495). The influence of recipients’ breed (Holstein or other), recipients’ lactation number (heifers or cows), estrus type (natural or synchronized), CL type (homogeneous, CLhom or cavitary, CLcav), CL side (left or right), volume of the CL and season of transfer (spring, autumn or winter) on pregnancy rates were determined. The pregnancy rates were analyzed by binomial logistic regression with IBM SPSS statistics software, version 26. Pregnancy rates after ET to Holstein cows and other breeds were 43.49% and 42.68%, respectively (p > 0.05). Regarding age, pregnancy rates were 45.56% for heifers and 30.77% for cows (p < 0.05). Pregnancy rates following ET during natural and synchronized estrus were 44.41% and 41.5%, respectively (p > 0.05). Pregnancy rates with a left- or right-side CL were 40.18% and 45.65%, respectively (p > 0.05). The pregnancy rates achieved with a CLhom and CLcav were 44.44% and 39.68%, respectively (p < 0.05). The rates obtained in spring, autumn and winter were 49.26%, 46.02% and 34.64%, respectively (p < 0.05). Moreover, it was found that pregnancy rates were higher in recipients with a CL volume measuring greater than 10 cm3 compared with those with a CL volume measuring less than 10 cm3 (p < 0.05). The comparisons showed that recipients’ breed, estrus type or side of the CL had no effect, but the recipients’ lactation number, ET season and the type and volume of the CL have significant effects on pregnancy rates during ET. Full article
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17 pages, 1520 KiB  
Article
The Transcript Levels and the Serum Profile of Biomarkers Associated with Clinical Endometritis Susceptibility in Buffalo Cows
by Ahmed El-Sayed, Salah H. Faraj, Basma H. Marghani, Fatmah A. Safhi, Mohamed Abdo, Liana Fericean, Ioan Banatean-Dunea, Cucui-Cozma Alexandru, Ahmad R. Alhimaidi, Aiman A. Ammari, Attia Eissa and Ahmed Ateya
Vet. Sci. 2024, 11(8), 340; https://doi.org/10.3390/vetsci11080340 - 27 Jul 2024
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Abstract
Determining the gene expression and serum profile of the indicators linked to clinical endometritis susceptibility in Egyptian buffalo cows was the aim of this investigation. The buffalo cows that were enrolled were divided into two groups: forty infected buffalo cows with clinical endometritis [...] Read more.
Determining the gene expression and serum profile of the indicators linked to clinical endometritis susceptibility in Egyptian buffalo cows was the aim of this investigation. The buffalo cows that were enrolled were divided into two groups: forty infected buffalo cows with clinical endometritis and forty seemingly healthy buffalo cows that served as the control group. For the purposes of gene expression and biochemical analysis, ten milliliters of blood was obtained via jugular venipuncture from each buffalo cow. TLR4, IL-8, IL-17, NFKB, SLCA11A1, NCF4, Keap1, HMOX1, OXSR1, ST1P1, and SERP1 were manifestly expressed at much higher levels in the buffaloes with endometritis. On the other hand, the genes that encode SOD, CAT, NDUFS6, Nrf2, and PRDX2 were down-regulated. There was a significant (p < 0.05) elevation of the serum levels of non-esterified fatty acids (NEFAs), beta hydroxy butyric acid (BHBA), triglycerides (TGs), globulin, creatinine, and cortisol, along with a reduction in the serum levels of glucose, cholesterol, total protein albumin, urea, estrogen (E2), progesterone (P4), follicle-stimulating hormone (FSH), luteinizing hormone (LH), thyroxine (T4), prostaglandin F2 α (PGF2α), calcium, iron, and selenium, in the endometritis group in comparison with the control. However, no significant change was observed in the values of phosphorus, magnesium, copper, or zinc in either group. Within the selective breeding of naturally resistant animals, the variation in the genes under study and the changes in the serum profiles of the indicators under investigation may serve as a reference guide for reducing endometritis in Egyptian buffalo cows. Full article
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11 pages, 935 KiB  
Article
Determination of Rhythmicity and Gestational Stage-Related Distribution of Blood Plasma Melatonin Concentrations in Donkey Mares
by Levente Harmat, János Nagy, Bence Somoskői, Alán Alpár, Sándor György Fekete and András Gáspárdy
Vet. Sci. 2024, 11(7), 310; https://doi.org/10.3390/vetsci11070310 - 11 Jul 2024
Viewed by 849
Abstract
The aim of this study is to obtain a more complete picture of blood plasma melatonin concentrations in the donkey mares. To this purpose, sampling and statistical processing were carried out in such a way that allowed the researchers to establish the annual [...] Read more.
The aim of this study is to obtain a more complete picture of blood plasma melatonin concentrations in the donkey mares. To this purpose, sampling and statistical processing were carried out in such a way that allowed the researchers to establish the annual and daily rhythms. Based on human observations, according to the hypothesis of the authors, the blood plasma melatonin concentration of pregnant individuals rises during the late gestational period, before parturition. To confirm this, the melatonin concentrations of pregnant and non-pregnant jennies were monitored and compared. In regard to the circannual rhythm, the significantly lowest midnight melatonin value (27.67 pg mL1) was typical for the summer solstice. Under consideration of circadian changes, a significantly strongest melatonin production (45.16 pg mL1) was observed on the night of the winter solstice (p < 0.001). Considering gestational age, the blood plasma melatonin concentration (around 38 pg mL1) does not change as gestation progresses (p = 0.136). The results obtained in this studied population of the domestic ass usefully expand the little knowledge previously gathered about the development of the blood plasma melatonin concentrations of this species. Full article
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14 pages, 18203 KiB  
Article
Exploring the Molecular Characteristics and Role of PDGFB in Testis and Epididymis Development of Tibetan Sheep
by Haolin Chen, Ling Pu, Chengcheng Tian, Xingcai Qi, Juanjuan Song, Yan Liao, Bentian Mo and Taotao Li
Vet. Sci. 2024, 11(6), 266; https://doi.org/10.3390/vetsci11060266 - 9 Jun 2024
Viewed by 889
Abstract
Platelet-derived growth factor B (PDGFB), as an important cellular growth factor, is widely involved in the regulation of cellular events such as cell growth, proliferation, and differentiation. Although important, the expression characteristics and biological functions in the mammalian reproductive system remain poorly understood. [...] Read more.
Platelet-derived growth factor B (PDGFB), as an important cellular growth factor, is widely involved in the regulation of cellular events such as cell growth, proliferation, and differentiation. Although important, the expression characteristics and biological functions in the mammalian reproductive system remain poorly understood. In this study, the PDGFB gene of Tibetan sheep was cloned by RT-PCR, and its molecular characteristics were analyzed. Subsequently, the expression of the PDGFB gene in the testes and epididymides (caput, corpus, and cauda) of Tibetan sheep at different developmental stages (3 months, 1 year, and 3 years) was examined by qRT-PCR and immunofluorescence staining. A bioinformatic analysis of the cloned sequences revealed that the CDS region of the Tibetan sheep PDGFB gene is 726 bp in length and encodes 241 amino acids with high homology to other mammals, particularly goats and antelopes. With the increase in age, PDGFB expression showed an overall trend of first decreasing and then increasing in the testis and epididymis tissues of Tibetan sheep, and the PDGFB mRNA expression at 3 months of age was extremely significantly higher than that at 1 and 3 years of age (p < 0.05). The PDGFB protein is mainly distributed in testicular red blood cells and Leydig cells in Tibetan sheep at all stages of development, as well as red blood cells in the blood vessel, principal cells, and the pseudostratified columnar ciliated epithelial cells of each epididymal duct epithelium. In addition, PDGFB protein expression was also detected in the spermatocytes of the 3-month-old group, spermatids of the 1-year-old group, spermatozoa and interstitial cells of the 3-year-old group, and loose connective tissue in the epididymal duct space in each developmental period. The above results suggest that the PDGFB gene, as an evolutionarily conserved gene, may play multiple roles in the development and functional maintenance of testicular cells (such as red blood cells, Leydig cells, and germ cells) and epididymal cells (such as red blood cells, principal cells, and ciliated epithelial cells) during testicular and epididymal development, which lays a foundation for the further exploration of the mechanisms by which the PDGFB gene influences spermatogenesis in Tibetan sheep. Full article
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