Search Results (19)

Dalbergia sissoo is a commercially exploited timber tree also known for its varied phytochemical constituents holding significant importance in folk medicines with documented biological properties. The present study reports the establishment of callus cultures from its leaf explants for the in vitro production of skin therapeutics. The growth parameters of the callus cultures were calculated. The antioxidant potential of the methanolic extracts of leaf and its callus cultures was evaluated through DPPH assay. Calli at third subculture stage showed the highest antioxidant potential (IC₅₀ 273 ± 14.14 µg/mL). A comparative analysis of phytochemical composition was performed using Gas Chromatography–Mass Spectrometry (GC-MS) which revealed the presence of potential skin therapeutic compounds. Out of 146 compounds, only 15 are unique to leaf explants, with the rest being produced in callus cultures. ADME predictions of potential compounds showed their drug likeness properties. The molecular docking of selected phytochemicals such as Chondrillasterol, Stearic acid, and n-Hexadecanoic acid against the tyrosinase enzyme showed better binding affinities than the reference drug (Kojic acid). Molecular dynamics simulation also showed stable conformations of the docked complexes with the target protein. Overall, these investigations unveil for the first time the successful in vitro production of skin therapeutics from D. sissoo, ensuring the sustainable and conservation-friendly utilization of its biomass for medicinal purposes. Full article

Urban green spaces are increasingly recognized for their potential to mitigate climate change by reducing atmospheric concentrations of greenhouse gases, especially carbon dioxide (CO₂). However, enhancing carbon sequestration efficiency in limited urban green areas remains a significant challenge for sustainable urban planning. Trees are among the most cost-effective and efficient natural carbon sinks, surpassing other types of land cover in terms CO₂ absorption and storage. The present study aimed to evaluate the carbon sequestration potential of four native tree species, Pongamia pinnata, Azadirachta indica, Melia azedarach, and Dalbergia sissoo, in urban parks across Multan City, Pakistan. A total of 456 trees of selected species within six parks of Multan City were inventoried to estimate the biomass and carbon stock using species-specific allometric equations. Soil organic carbon at two soil depths beneath the canopy of each tree was also estimated using Walkley–Black method. The findings revealed that the highest mean tree biomass (2.16 Mg ha⁻¹), carbon stock (1.04 Mg ha⁻¹) and carbon sequestration (3.80 Mg ha⁻¹) were estimated for Dalbergia sissoo, while Melia azedarach exhibited the lowest (0.12 Mg ha⁻¹, 0.06 Mg ha⁻¹ & 0.23 Mg ha⁻¹, respectively) across all six parks. The soil carbon stocks ranged from 48.86 Mg ha⁻¹ to 61.68 Mg ha⁻¹ across all study sites. These findings emphasize the importance of species selection in urban green planning for carbon sequestration. Strategic planting of effective native trees like Dalbergia sissoo can mitigate climate change and provide urban forest ecosystem services. Full article

The synergetic strategy has created tremendous advantages in drug-resistance bacterial infection treatment, whereas challenges related to novel compound discovery and identifying drug-binding targets still remain. The mechanisms of antimicrobial resistance involving β-lactamase catalysis and the degradation of β-lactam antibiotics are being revealed, with relevant therapies promising to improve the efficacy of existing major classes of antibiotics in the foreseeable future. In this study, it is demonstrated that nordalbergin, a coumarin isolated from the wood bark of Dalbergia sissoo, efficiently potentiated the activities of β-lactam antibiotics against methicillin-resistant Staphylococcus aureus (MRSA) by suppressing β-lactamase performance and improving the bacterial biofilm susceptibility to antibiotics. Nordalbergin was found to destabilize the cell membrane and promote its permeabilization. Moreover, nordalbergin efficiently improved the therapeutic efficacy of amoxicillin against MRSA pneumonia in mice, as supported by the lower bacterial load, attenuated pathological damage, and decreased inflammation level. These results demonstrate that nordalbergin might be a promising synergist of amoxicillin against MRSA infections. This study provided a new approach for developing potentiators for β-lactam antibiotics against MRSA infections. Full article

Salinity is one of the most significant abiotic stress that affects the growth and development of high-value tree species, including sandalwood, which can also be managed effectively on saline soils with the help of suitable host species. Therefore, the current investigation was conducted to understand the physiological processes and antioxidant mechanisms in sandalwood along the different salinity gradients to explore the host species that could support sandalwood growth in salt-affected agro-ecosystems. Sandalwood seedlings were grown with ten diverse host species with saline water irrigation gradients (EC_iw~3, 6, and 9 dS m⁻¹) and control (EC_iw~0.82 dS m⁻¹). Experimental findings indicate a decline in the chlorophyll content (13–33%), relative water content (3–23%), photosynthetic (27–61%) and transpiration rate (23–66%), water and osmotic potential (up to 137%), and ion dynamics (up to 61%) with increasing salinity levels. Conversely, the carotenoid content (23–43%), antioxidant activity (up to 285%), and membrane injury (82–205%) were enhanced with increasing salinity stress. Specifically, among the hosts, Dalbergia sissoo and Melia dubia showed a minimum reduction in chlorophyll content, relative water content, and plant water relation and gas exchange parameters of sandalwood plants. Surprisingly, most of the host tree species maintained K⁺/Na⁺ of sandalwood up to moderate water salinity of EC_iw~6 dS m⁻¹; however, a further increase in water salinity decreased the K⁺/Na⁺ ratio of sandalwood by many-fold. Salinity stress also enhanced the antioxidative enzyme activity, although the maximum increase was noted with host plants M. dubia, followed by D. sissoo and Azadirachta indica. Overall, the investigation concluded that sandalwood with the host D. sissoo can be successfully grown in nurseries using saline irrigation water and, with the host M. dubia, it can be grown using good quality irrigation water. Full article

Forests face a variety of threats in the modern era. Agroforestry systems, both traditional and introduced, have a tremendous capacity for providing sustainable resources and combating the impact of global climate change. Indigenous agroforestry and forest land-use systems are important reservoirs for biodiversity conservation and ecosystem services, providing a potential contribution to livelihood security for rural communities. This study aimed to assess the tree diversity and carbon stock of agroforestry and adjoining forests along altitudinal gradients, ranging between 700 and 2200 masl (i.e., lower, middle, and upper altitudes) by laying sample plots randomly of a size of 20 × 20 m². In the forest land-use system, the maximum Importance Value Index (IVI) included Dalbergia sissoo (71.10), Pyrus pashia (76.78), and Pinus roxburghii (79.69) at the upper, middle, and lower elevations, respectively, whereas, in the agroforestry land-use system, the IVI reported for Ficus semicordata was 43.05 at the upper, while for Grewia optiva it was at 53.82 at the middle and 59.33 at the lower altitudes. The below-ground biomass density (AGBD) was recorded as 1023.48 t ha⁻¹ (lower), 242.92 t ha⁻¹ (middle), and 1099.35 t ha⁻¹(upper), while in the agroforestry land-use system, the AGBD was 353.48 t ha⁻¹ (lower), 404.32 t ha⁻¹ (middle), and 373.23 t ha⁻¹ (upper). The total carbon density (TCD) values recorded were 630.57, 167.32, and 784.00 t ha⁻¹ in forest land-use systems, and 227.46, 343.23, and 252.47 in agroforestry land-use systems for lower, middle, and upper altitudes, respectively. The Margalef’s Index values for agroforestry and forests ranged from 2.39 to 2.85 and 1.12 to 1.30, respectively. Soil organic carbon (SOC) stock recorded 45.32, 58.92, and 51.13 Mg C ha⁻¹ for agroforestry and 61.73, 42.65, and 71.08 Mg C ha⁻¹ for forest in lower, middle and upper elevations, respectively. The study suggests that selecting land use patterns can be an effective management system for tree species at different elevations for carbon storage, helping to mitigate climate change and aiding in sustainable management of ecosystems in the Garhwal Himalayas. Full article

Heavy metals are notoriously toxic pollutants which can potentially harm living beings and are serious health hazards. The aim of the present study was to assess the levels of cadmium (Cd) and nickel (Ni) throughout the year in the urban areas of the densely populated industrial city of Faisalabad in eight commonly raised ornamental tree species with phytoremediation potential. High levels of Cd and Ni were recorded in all study areas, with spatio-temporal heterogeneity. Heavy metal uptake varied among plant species with Cd and Ni in soil ranging between 6.78–8.57 mgkg⁻¹ and 46.31–55.85 mg kg⁻¹ respectively. Plant species accumulated 6.73–8.98 mg kg⁻¹ Cd and 26.42–52.50 mg kg⁻¹ Ni with Conocarpus erectus, Dalbergia sissoo and Bismarckia nobilis showing higher accumulation potential than others. Dalbergia sissoo accumulated the highest levels of Ni and was shown to a good bio-indicator for this metal. The highest accumulation of Cd was recorded in Conocarpus erectus (9 mg kg⁻¹), followed by Dalbergia sissoo (8.2 mg kg⁻¹) and Bismarckia nobilis (8.1 mg kg⁻¹) while the leaves of Azadirachta indica retained the lowest (6.3 mg kg⁻¹) Cd levels. The highest levels of metals were accumulated by all species during the summer season while the second highest were observed in the autumn season. The study revealed that ornamental species can help in minimizing heavy metal pollution as well as providing green space in urban settings for maintaining a clean and healthy environment. Full article

Microglia-associated neuroinflammation is recognized as a critical factor in the pathogenesis of neurodegenerative diseases; however, there is no effective treatment for the blockage of neurodegenerative disease progression. In this study, the effect of nordalbergin, a coumarin isolated from the wood bark of Dalbergia sissoo, on lipopolysaccharide (LPS)-induced inflammatory responses was investigated using murine microglial BV2 cells. Cell viability was assessed using the MTT assay, whereas nitric oxide (NO) production was analyzed using the Griess reagent. Secretion of interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) was detected by the ELISA. The expression of inducible NO synthase (iNOS), cyclooxygenase (COX)-2, mitogen-activated protein kinases (MAPKs) and NLRP3 inflammasome-related proteins was assessed by Western blot. The production of mitochondrial reactive oxygen species (ROS) and intracellular ROS was detected using flow cytometry. Our experimental results indicated that nordalbergin ≤20 µM suppressed NO, IL-6, TNF-α and IL-1β production; decreased iNOS and COX-2 expression; inhibited MAPKs activation; attenuated NLRP3 inflammasome activation; and reduced both intracellular and mitochondrial ROS production by LPS-stimulated BV2 cells in a dose-dependent manner. These results demonstrate that nordalbergin exhibits anti-inflammatory and anti-oxidative activities through inhibiting MAPK signaling pathway, NLRP3 inflammasome activation and ROS production, suggesting that nordalbergin might have the potential to inhibit neurodegenerative disease progression. Full article

Plant responses to air pollution have been extensively studied in urban environments. Nevertheless, detailed and holistic studies assessing their retaliation to air contaminants are still limited. The present study evaluates the effect of criteria pollutants (SO₂, NO₂, PM₁₀ and O₃) on the overall biochemistry and resource allocation strategy of plants in order to categorize the dominant roadside species (Mangifera indica, Psidium guajava, Ficus religiosa, Azadirachta indica, Dalbergia sissoo, Cascabela thevetia and Bougainvillea spectabilis) of the Indo-Gangetic Plains (IGP), with different morphologies and habits, into species that are tolerant and sensitive to the prevailing air pollutants. This study was performed at three different land-use sites (industrial, commercial and reference) in Varanasi for two seasons (summer and winter). It was inferred that NO₂ and PM₁₀ consistently violated the air quality standards at all the sites. The fifteen assessed parameters reflected significant variations depending upon the site, season and plant species whereupon the enzymatic antioxidants (superoxide dismutase and catalase) and resource utilization parameters (leaf area and leaf dry matter content) were remarkably affected. Based on the studied parameters, it was entrenched that deciduous tree species with compound leaves (D. sissoo > A. indica) were identified as the less sensitive, followed by a shrub (C. thevetia > B. spectabilis), while evergreen species with simple leaves were the most sensitive. It was also substantiated that the morphology of the foliage contributed more toward the differential response of the plants to air pollutants than its habit. Full article

Biochar (BC) applications have multiple impacts on crops’ nutrient availability, growth and yield depending on the feedstock type and pyrolysis conditions. Pot and field experiments were conducted to examine the effects of biochars (BCs) prepared from three different feedstocks, Acacia modesta wood biochar (AWB), Dalbergia sissoo wood biochar (DWB) and poultry litter biochar (PLB), on soil’s nutrient availability, uptake by wheat (Triticum aestivum) and sunflower (Helianthus annuus) crops and their yield attributes. All BCs were applied at the rate of 10t ha⁻¹ in each treatment in both experiments, and pot and field trials were designed according to a two-factor factorial completely randomized design (CRD) and two-factor factorial randomized complete block design (RCBD), respectively. The concentration of soil NO₃-N, NH₄-N, Olsen P and extractable K increased by 98.5, 296, 228 and 47%, respectively, in the pot experiment with the application of PLB+polyhalite (PH) treatments. Similarly, in field experiments, NO₃-N, NH₄-N and Olsen P contents increased by 91, 268 and 156% under the PLB+PH treatment, respectively. However, in both experiments, soil’s microbial biomass phosphorus (MBP) was significantly higher after AWB+PH treatment, and the increments were 127 and 109% while microbial biomass nitrogen (MBN) contents were 16 and 14% higher than the control under DWB+PH and AWB+PH treatments, respectively, in the field experiment. Similarly, combined PLB+PH increased the total organic carbon (TOC) of soil by 193%. Moreover, PLB+PH co-applications with PH significantly increased sunflower grain yields by up to 58% and the harvest index by 45%. Overall, no negative impact with respect to BCs was observed on the soil’s nutrient content and plant growth. Hence, for immediate crop benefits and soil health, using nutrient biochar (PLB) alone or in combination with chemical fertilizers is recommended. Full article

In this study, cerium oxide nanorods (CeO₂-NRs) were synthesized by using the phytochemicals present in the Dalbergia sissoo extract. The physiochemical characteristics of the as-prepared CeO₂-NRs were investigated by using ultraviolet-visible spectroscopy (UV-VIS), scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), and X-ray diffraction analysis (XRD). The SEM and UV-VIS analyses revealed that the acquired nanomaterials possessed a rod-like morphology while the XRD results further confirmed that the synthesized NRs exhibited a cubic crystal lattice system. The antioxidant capacity of the synthesized CeO₂-NRs was investigated by using several in vitro biochemical assays. It was observed that the synthesized NRs exhibited better antioxidant potential in comparison to the industrial antioxidant of the butylated hydroxyanisole (BHA) in 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay. The biochemical assays, including lipid peroxidation (LPO), total antioxidant capacity (TAC), and catalase activity (CAT), were also performed in the human lymphocytes incubated with the CeO₂-NRs to investigate the impact of the NRs on these oxidative biomarkers. Enhanced reductive capabilities were observed in all the assays, revealing that the NRs possess excellent antioxidant properties. Moreover, the cytotoxic potential of the CeO₂-NRs was also investigated with the MTT assay. The CeO₂-NRs were found to effectively kill off the cancerous cells (MCF-7 human breast cancer cell line), further indicating that the synthesized NRs exhibit anticancer potential as well. One of the major applications studied for the prepared CeO₂-NRs was performing the statistical optimization of the photocatalytic degradation reaction of the methyl orange (MO) dye. The reaction was optimized by using the technique of response surface methodology (RSM). This advanced approach facilitates the development of the predictive model on the basis of central composite design (CCD) for this degradation reaction. The maximum degradation of 99.31% was achieved at the experimental optimized conditions, which corresponded rather well with the predicted percentage degradation values of 99.58%. These results indicate that the developed predictive model can effectively explain the performed experimental reaction. To conclude, the CeO₂-NRs exhibited excellent results for multiple applications. Full article

This research estimates the carbon stock of the subtropical broad-leaved evergreen scrub forest of Lehtrar, a revenue estate of Kotli Sattian, Rawalpindi, Punjab, Pakistan. A total of six nested co-centric plots of 17.84 m² each were laid out in the forest, having two sub-plots of 5.64 m² and 1 m² each, for shrubs and litter, respectively. Stem density, tree height, diameter at breast height (DBH), total tree biomass, and total carbon stock were calculated. In each plot, parameters like latitude, longitude, aspect, slope, elevation, tree count, etc., were catalogued. The carbon value was calculated in pools such as aboveground biomass (AGB), belowground biomass (BGB), litter, shrubs, etc. The tree height was measured using Abney’s level and the diameter at breast height (DBH) with diameter tape, while factors such as volume, shrub mass, litter mass, total tree biomass, and total carbon stock were calculated by using standard formulas. Results showed Olea ferrugineae to be the most abundant tree species in the study area, followed by Acacia modesta. The total average DBH and height were calculated as 17.03 and 16.79, respectively, with the species Dalbergia sissoo having the greatest DBH value. The mean carbon stock came out to be 47.75 tons/ha, with plot number 3 having the highest value of carbon stock, owing to the greatest stem count. The results of the study were significant and reflected a rich stem density, rich biomass, and an adequate carbon stocking capacity. The scrub forests of the study area, being important carbon sinks, are prone to deforestation and forest degradation activities that need to be controlled by using proper forest management practices to keep their carbon sequestration ability intact, as suggested under various reducing emissions from deforestation and forest degradation (REDD initiatives of UNFCCC. Full article

Dalbergia sissoo is a woody plant with economic and medicinal value. As the pharmacological qualities and properties of the wood from this plant primarily depend on its extractives, in this study, the metabolomic analysis of extractives from its stems was carried out using UPLC-MS/MS. A total of 735 metabolites were detected from two groups of samples, heartwood and sapwood, with the largest number of terpenoids in type and the largest number of flavonoids in quantity. The PCA and cluster analysis showed significant differences in the metabolite composition between the two groups. The differential metabolites were mainly organic oxygen compounds, flavonoids, and isoflavones. Among the 105 differential metabolites, 26 metabolites were significantly higher in relative content in sapwood than in heartwood, while the other 79 metabolites were significantly higher in relative content in heartwood than in sapwood. KEGG metabolic pathway enrichment analysis showed that these differential metabolites were mainly enriched in three metabolic pathways: Flavonoid biosynthesis, isoflavonoid biosynthesis, and flavonoid and flavonol biosynthesis. This study provides a reference for metabolomics studies in Dalbergia and other woody plants. Full article

Study Design: Randomized Control Trial. Objective: A randomized control trial was planned to aim to assess whether subcutaneous Injection of Teriparatide and Tablet Reunion (combination of Cissus Quadrangularis and Dalbergia sissoo) improves maxillofacial fracture healing as compared to the control group. Methods: 24 patients of mandibular fracture with or without concomitant maxillofacial fractures were randomly divided into 3 equal groups (Group 1—Control, Group 2—Tablet Reunion, and Group 3—Injection Teriparatide) and the treatment duration was 4 weeks. Pain, fracture site mobility, bite force, serum markers, and radiographic healing were assessed preoperatively and postoperatively at regular intervals till 12 weeks. Results: Group 2 showed early pain relief, although it was insignificant. Group 3 showed the highest anterior bite force at all the time points. Change in mean posterior bite force (PBF) showed a statistically significant increase at 8th week and 12th week in intergroup comparison; however, at 12thweek, Group 3was significantly better than Group 1 and reported the highest posterior bite force compared to other groups. Serum calcium and PTH level showed no significant difference, whereas SerumALP showed a statistically significant increase in Group 3. The radiographic assessment showed no significant difference among the 3 groups. Conclusions: Both the intervention group drugs showed a promising effect on accelerating the fracture healing and improving bite force restoration with the osteoanabolic action; however, early radiographic healing and increased serum osteogenic markers in Group 3 indicate its possible optimistic role in maxillofacial fracture healing. Full article

Vachellia nilotica (L.) P.J.H. Hurther & Mabb. and Dalbergia sissoo Roxb. are two of the most important multipurpose agroforestry tree species of the Indian sub-continent, but their growth in saline soils is greatly reduced. Recently, organic amendments have showed the potential to increase plant growth in salt-affected soils; however, the influence of using these amendments for growing the above-mentioned tree species under saline conditions is not yet quantified. Therefore, an experiment was devised to analyze the interactive effects of organic amendments in saline soils on the growth of V. nilotica and D. sissoo. Under controlled conditions, a pot experiment was conducted in sandy loam saline soils (EC = 20.5 dSm⁻¹). Organic amendments from four diverse sources: farmyard manure (FYM), poultry manure (PM), slurry (SL), and farmyard manure biochar (FYMB) were employed in this study. At the harvesting time, data regarding morphological, physiological, ionic, and biochemical parameters were obtained. The current study results indicated that both tree species reacted differently, but positively, to diverse applied amendments. The maximum increment in total above-ground biomass, total below-ground biomass, and shoot length for V. nilotica (163.8%, 116.3%, and 68.2%, respectively) was observed in FYM amended soils, while the maximum increment for D. sissoo (128%, 86%, and 107%, respectively) was observed in FYMB amended soils, as compared to control. Minimum plant growth of both species was observed in untreated soils (saline soils). Likewise, the maximum potassium ion and minimum sodium ion concentrations were present in the root and shoots of plants (both species) treated with FYMB. The use of organic amendments resulted in decreased concentrations of malondialdehyde and hydrogen peroxide, and increased concentrations of antioxidant enzymes such as SOD, POD, and CAT. Moreover, higher photosynthetic rates and stomatal conductance were observed in the plants grown in amended soils. The findings of this study can be used to include the above-mentioned high-value tree species for future afforestation programs under saline conditions. Full article

Heartwood extracts of naturally durable wood species are often evaluated as alternatives to chemical wood preservatives, but field data from long-term performance testing are lacking. The current study evaluated the long-term (five-year) performance of two non-durable wood species treated with heartwood extracts of either Tectona grandis, Dalbergia sissoo, Cedrus deodara, or Pinus roxburghii alone or combined with linseed oil. Stakes (45.7 × 1.9 × 1.9 cm) and blocks (12.5 × 3.75 × 2.5 cm) cut from the sapwood of cottonwood and southern pine were vacuum-pressure impregnated with the individual heartwood species extract, linseed oil, or a mixture of each individual wood extract and linseed oil. For comparison, solid heartwood stakes and blocks of the wood species used to obtain extracts were also included in the tests. All samples were exposed for five years to decay and termites at a test site in southern Mississippi using ground contact (AWPA E7) and ground proximity (AWPA E26) tests. Results showed that extract-oil mixtures imparted higher termite and decay resistance in cottonwood and southern pine than linseed oil only or the individual heartwood species extract in both tests. However, these treatments were as not effective as to commercially used wood preservatives, copper naphthenate (CuN) or disodium octaborate tetrahydrate (DOT) in either test. Moreover, solid heartwood P. roxburghii stakes were completely decayed and attacked by termites after five years in the ground contact test. In contrast, C. deodara stakes were slightly attacked by termites and moderately attacked by decay fungi. However, T. grandis and D. sissoo stakes showed slight to superficial attack by termites and decay fungi in ground contact test. In contrast, T. grandis and D. sissoo blocks showed slight decay fungi attack in above-ground tests. However, termites did not attack T. grandis, D. sissoo, and C. deodara blocks. However, decay fungi moderately attacked C. deodara blocks, and P. roxburghii blocks were severely attacked by decay fungi and termites in the above-ground test. Full article