Search Results (27)

Elevated temperatures have been shown to decrease muscle force production, with potential causes including protein unfolding, enzyme denaturation, and reactive oxygen species (ROS). This study aimed to investigate whether fucoidan, a compound derived from brown seaweed, could mitigate heat-stress-induced loss of muscle function. C57BL/6 mice were orally administered fucoidan (400 mg/kg/day) from one of two different seaweed species Fucus vesiculosus (FVF) or Undaria pinnatifida (UPF) or vehicle control for seven consecutive days. Subsequently, the in vitro muscle function of the fast-twitch extensor digitorum longus (EDL) was assessed at either 25 °C (control) or 43 °C (heat stress). Functional analysis was complemented with gene analysis and the C2C12 myoblast heat-stress assay. The temperature (43 °C)-induced loss of force produced by the EDL muscle was significantly attenuated by fucoidan from FVF but not UPF. Fucoidan from UPF did not affect gene expression levels, whereas fucoidan from FVF significantly increased the expression levels of HSP90. In mouse C2C12 myoblasts, heat stress induced a significant increase in ROS production which was significantly reduced by both fucoidan species. These results suggest fucoidan extracted from Fucus vesiculosus may be an effective preventive strategy to protect against heat-induced loss of muscle strength in fast-twitch muscles. Full article

The study of drugs of natural origin that increase endurance and/or accelerate recovery is an integral part of sports medicine and physiology. In this paper, decaffeinated green tea extract (GTE) and two ammonium salts—chloride (ACL) and carbonate (ACR)—were tested individually and in combination with GTE as stimulants of physical performance in a forced swimming rat experimental model. The determined parameters can be divided into seven blocks: functional (swimming duration); biochemistry of blood plasma; biochemistry of erythrocytes; hematology; immunology; gene expression of slow- and fast-twitch muscles (m. soleus, SOL, and m. extensor digitorum longus, EDL, respectively); and morphometric indicators of slow- and fast-twitch muscles. Regarding the negative control (intact animals), the maximum number of changes in all blocks of indicators was recorded in the GTE + ACR group, whose animals showed the maximum functional result and minimum lactate values on the last day of the experiment. Next, in terms of the number of changes, were the groups ACR, ACL, GTE + ACL, GTE and NaCl (positive control). In general, the number of identified adaptive changes was proportional to the functional state of the animals of the corresponding groups, in terms of the duration of the swimming load in the last four days of the experiment. However, not only the total number but also the qualitative composition of the identified changes is of interest. The results of a comparative analysis suggest that, in the model of forced swimming we developed, GTE promotes restoration of the body and moderate mobilization of the immune system, while small doses of ammonium salts, especially ammonium carbonate, contribute to an increase in physical performance, which is associated with satisfactory restoration of skeletal muscles and the entire body. The combined use of GTE with ammonium salts does not give a clearly positive effect. Full article

Zeta potential (ζ potential) is a significant parameter to characterize the electric property of the electric double layer (EDL), which is important at the solid–liquid interface. Non-uniform ζ potential could be developed on a chemically uniform solid–liquid interface due to external flow. However, its influence on the flow has never been concerned. In this investigation, we numerically studied the influence of non-uniform 2D ζ potential on the flow at the solid–liquid interface. It is found, that even without any external electric field and only considering the influence of 2D ζ potential distribution, swirling flow can be generated near EDL, according to the rotational electric volume force. The streamwise vortices, which are important in the turbulent boundary layer, are theoretically predicted in this laminar flow model when considering the 2D distribution of ζ potential, implying the necessity of considering the origin of streamwise vortices of the turbulent boundary layer from the perspective of electrokinetic flow. In addition, the ζ potential distribution can promote the wall shear stress. Therefore, more attention must be paid to shear-sensitivity circumstances, like biomedical, medical devices, and in vivo. We hope that the current investigation can help us to better understand the effect of charge distribution on interfacial flow and provide theoretical guidance for the development of related applications in the future. Full article

The recessive Ryanodine Receptor Type 1 (RyR1) P3527S mutation causes mild muscle weakness in patients and increased resting cytoplasmic [Ca²⁺] in transformed lymphoblastoid cells. In the present study, we explored the cellular/molecular effects of this mutation in a mouse model of the mutation (RyR1 P3528S). The results were obtained from 73 wild type (WT/WT), 82 heterozygous (WT/MUT) and 66 homozygous (MUT/MUT) mice with different numbers of observations in individual data sets depending on the experimental protocol. The results showed that WT/MUT and MUT/MUT mouse strength was less than that of WT/WT mice, but there was no difference between genotypes in appearance, weight, mobility or longevity. The force frequency response of extensor digitorum longus (EDL) and soleus (SOL) muscles from WT/MUT and MUT/MUT mice was shifter to higher frequencies. The specific force of EDL muscles was reduced and Ca²⁺ activation of skinned fibres shifted to a lower [Ca²⁺], with an increase in type I fibres in EDL muscles and in mixed type I/II fibres in SOL muscles. The relative activity of RyR1 channels exposed to 1 µM cytoplasmic Ca²⁺ was greater in WT/MUT and MUT/MUT mice than in WT/WT mice. We suggest the altered RyR1 activity due to the P2328S substitution could increase resting [Ca²⁺] in muscle fibres, leading to changes in fibre type and contractile properties. Full article

Mammalian hibernation is composed of multiple episodes of torpor bout, separated by phases of interbout arousal. During torpor, the skeletal muscles of mammals are undoubtedly inactive, but it has been proven to mitigate disuse atrophy. While interbout arousal has been implicated in the prevention of muscle atrophy, the underlying mechanisms sustaining muscle contraction remain to be explored. In the present study, Daurian ground squirrels (Spermophilus dauricus) were divided into four groups: pre-hibernation (PRE), torpor (TOR), interbout arousal (IBA), and post-hibernation (POST). The contractile performance of slow-twitch soleus muscle (SOL) and fast-twitch extensor digitorum longus muscle (EDL) was detected both in situ and in vitro. Concurrently, mitochondrial respiratory chain complex activity in these muscles was quantified. Our findings revealed that in situ contractile properties of both muscles, including force, power output, time duration, and force development/relaxation rates of twitch contraction, and force and power output of tetanic contraction declined in the TOR group compared to the PRE group, but improved in the IBA and POST groups. Fatigue resistance of muscles, determined by the power output of repetitive tetanic contractions in situ, decreased in the TOR group but recovered in the IBA and POST groups. In vitro studies demonstrated that tetanic contraction power output in isolated muscles increased with muscle temperature in both TOR and IBA groups. However, at the same temperature, power output was consistently lower in the TOR group compared to the IBA group. Moreover, the activity of the mitochondrial respiratory chain complex, especially Complexes I and II, decreased in the TOR group but showed recovery in the IBA and POST groups. These findings suggest that both the contractile performance and fatigue resistance of mammalian skeletal muscle are compromised during torpor but can be improved during interbout arousal and post-hibernation. The rebound in body temperature and rise in mitochondrial respiratory chain complex activity in skeletal muscle are involved in enhancing contractile performance and fatigue resistance. This study suggests that interbout arousal functions as a vital temporal interval during which skeletal muscles can transition from the inactivity induced by torpor to a state of restored contractile functionality. Thus, interbout arousal serves as a behavioral safeguard against disuse-induced damage to skeletal muscles during hibernation. Full article

Equinus and equinovarus foot deviations (EVFD) are the most frequent lower limb acquired deformities in stroke survivors. We analysed the contribution that the tibialis anterior (TA), extensor digitorum longus (EDL) and plantarflexor muscles play in EVFD via a biomechanical musculoskeletal model of the ankle–foot complex. Our model was composed of 28 bones (connected by either revolute joints or bone surface contacts), 15 ligaments (modelled as non-linear springs), and 10 muscles, modelled as force actuators. Different combinations of muscle contractions were also simulated. Our results demonstrate that, compared to the condition when the foot is suspended off the ground, the contraction of the TA alone produces dorsiflexion (from −18° to 0°) and a greater supination/inversion (from 12° to 30°). The EDL alone produces dorsiflexion (from −18° to −6°), forefoot pronation (25°) and calcaneal eversion (5.6°). Only TA and EDL synergistic action can lead the foot to dorsiflexion suitable for most daily life activities (≥20°) without any deviation in the frontal plane. When pathological contractures of the plantarflexor muscles were simulated, foot deformities reproducing EVFD were obtained. These results can be relevant for clinical applications, highlighting the importance of EDL assessment, which may help to design appropriate functional surgery and plan targeted rehabilitation treatments. Full article

The remodelling of the extracellular matrix plays an important role in skeletal muscle development and regeneration. Syndecan-4 is a cell surface proteoglycan crucial for muscle differentiation. Syndecan-4^−/− mice have been reported to be unable to regenerate following muscle damage. To investigate the consequences of the decreased expression of Syndecan-4, we have studied the in vivo and in vitro muscle performance and the excitation–contraction coupling machinery in young and aged Syndecan-4^+/− (SDC4) mice. In vivo grip force was decreased significantly as well as the average and maximal speed of voluntary running in SDC4 mice, regardless of their age. The maximal in vitro twitch force was reduced in both EDL and soleus muscles from young and aged SDC4 mice. Ca²⁺ release from the sarcoplasmic reticulum decreased significantly in the FDB fibres of young SDC4 mice, while its voltage dependence was unchanged regardless of age. These findings were present in muscles from young and aged mice as well. On C2C12 murine skeletal muscle cells, we have also found altered calcium homeostasis upon Syndecan-4 silencing. The decreased expression of Syndecan-4 leads to reduced skeletal muscle performance in mice and altered motility in C2C12 myoblasts via altered calcium homeostasis. The altered muscle force performance develops at an early age and is maintained throughout the life course of the animal until old age. Full article

Adding potassium nitrate (KNO₃) to the diet improves the physiological properties of mammalian muscles (rebuilds weakened muscle, improves structure and functionality). The aim of this study was to investigate the effect of KNO₃ supplementation in a mouse model. BALB/c mice were fed a KNO₃ diet for three weeks, followed by a normal diet without nitrates. After the feeding period, the Extensor digitorum longus (EDL) muscle was evaluated ex vivo for contraction force and fatigue. To evaluate the possible pathological changes, the histology of EDL tissues was performed in control and KNO₃-fed groups after 21 days. The histological analysis showed an absence of negative effects in EDL muscles. We also analyzed 15 biochemical blood parameters. After 21 days of KNO₃ supplementation, the EDL mass was, on average, 13% larger in the experimental group compared to the controls (p < 0.05). The muscle-specific force increased by 38% in comparison with the control group (p < 0.05). The results indicate that KNO₃ has effects in an experimental mouse model, showing nitrate-diet-induced muscle strength. This study contributes to a better understanding of the molecular changes in muscles following nutritional intervention and may help develop strategies and products designated to treat muscle-related issues. Full article

Disuse muscle atrophy is usually accompanied by changes in skeletal muscle structure, signaling, and contractile potential. Different models of muscle unloading can provide valuable information, but the protocols of experiments with complete immobilization are not physiologically representative of a sedentary lifestyle, which is highly prevalent among humans now. In the current study, we investigated the potential effects of restricted activity on the mechanical characteristics of rat postural (soleus) and locomotor (extensor digitorum longus, EDL) muscles. The restricted-activity rats were kept in small Plexiglas cages (17.0 × 9.6 × 13.0 cm) for 7 and 21 days. After this, soleus and EDL muscles were collected for ex vivo mechanical measurements and biochemical analysis. We demonstrated that while a 21-day movement restriction affected the weight of both muscles, in soleus muscle we observed a greater decrease. The maximum isometric force and passive tension in both muscles also significantly changed after 21 days of movement restriction, along with a decrease in the level of collagen 1 and 3 mRNA expression. Furthermore, the collagen content itself changed only in soleus after 7 and 21 days of movement restriction. With regard to cytoskeletal proteins, in our experiment we observed a significant decrease in telethonin in soleus, and a similar decrease in desmin and telethonin in EDL. We also observed a shift towards fast-type myosin heavy chain expression in soleus, but not in EDL. In summary, in this study we showed that movement restriction leads to profound specific changes in the mechanical properties of fast and slow skeletal muscles. Future studies may include evaluation of signaling mechanisms regulating the synthesis, degradation, and mRNA expression of the extracellular matrix and scaffold proteins of myofibers. Full article

(Photo-)electrocatalytic artificial photosynthesis driven by electrical and/or solar energy that converts water (H₂O) and carbon dioxide (CO₂) into hydrogen (H₂), carbohydrates and oxygen (O₂), has proven to be a promising and effective route for producing clean alternatives to fossil fuels, as well as for storing intermittent renewable energy, and thus to solve the energy crisis and climate change issues that we are facing today. Basic (photo-)electrocatalysis consists of three main processes: (1) light absorption, (2) the separation and transport of photogenerated charge carriers, and (3) the transfer of photogenerated charge carriers at the interfaces. With further research, scientists have found that these three steps are significantly affected by surface and interface properties (e.g., defect, dangling bonds, adsorption/desorption, surface recombination, electric double layer (EDL), surface dipole). Therefore, the catalytic performance, which to a great extent is determined by the physicochemical properties of surfaces and interfaces between catalyst and reactant, can be changed dramatically under working conditions. Common approaches for investigating these phenomena include X-ray photoelectron spectroscopy (XPS), X-ray absorption spectroscopy (XAS), scanning probe microscopy (SPM), wide angle X-ray diffraction (WAXRD), auger electron spectroscopy (AES), transmission electron microscope (TEM), etc. Generally, these techniques can only be applied under ex situ conditions and cannot fully recover the changes of catalysts in real chemical reactions. How to identify and track alterations of the catalysts, and thus provide further insight into the complex mechanisms behind them, has become a major research topic in this field. The application of in situ/operando characterization techniques enables real-time monitoring and analysis of dynamic changes. Therefore, researchers can obtain physical and/or chemical information during the reaction (e.g., morphology, chemical bonding, valence state, photocurrent distribution, surface potential variation, surface reconstruction), or even by the combination of these techniques as a suite (e.g., atomic force microscopy-based infrared spectroscopy (AFM-IR), or near-ambient-pressure STM/XPS combined system (NAP STM-XPS)) to correlate the various properties simultaneously, so as to further reveal the reaction mechanisms. In this review, we briefly describe the working principles of in situ/operando surface/interface characterization technologies (i.e., SPM and X-ray spectroscopy) and discuss the recent progress in monitoring relevant surface/interface changes during water splitting and CO₂ reduction reactions (CO₂RR). We hope that this review will provide our readers with some ideas and guidance about how these in situ/operando characterization techniques can help us investigate the changes in catalyst surfaces/interfaces, and further promote the development of (photo-)electrocatalytic surface and interface engineering. Full article

At solid-liquid interfaces, charged particles within the electric double layer (EDL) are acted on by the electrostatic force, which may affect cell absorption and surface wettability. In this study, a model of the electrostatic force and surface tension of textured surfaces was presented. Then, the growth and adhesion of Murine osteoblasts (MC3T3-E1) cells on laser-ablated micro-square-textured Ti-6Al-4V surfaces were studied to demonstrate the use of a laser-processed texture to effectively improve bioactivity. Three different micro-square-textured hydrophilic surfaces, presenting lower contact angles of 19°, 22.5°, and 31.75° compared with that of a smooth surface (56.5°), were fabricated using a fiber-optic laser. Cellular morphology and initial cell attachment were analyzed by field emission scanning electron microscopy (SEM) and fluorescence microscopy, respectively. The results show that the electrostatic force not only made the textured surface more hydrophilic but also made the cells tend to adhere to the edges and corners of the protruding convexes. Cell morphology analysis also showed that cells would prefer to grow at the edges and corners of each micro-square convex protrusion. The laser-treated surfaces were more conducive to rapid cell growth and adhesion, and cells were preferentially attached on the hydrophilic-textured surfaces. Electrostatic force may be an important factor in effectively improving the bioactivity of Ti-6Al-4V surfaces, and the presence of more surface grooves would be more conducive to improving the bioactivity of cells. Full article

The present study provides analytical and numerical solutions for an electromagnetohydrodynamic (EMHD) flow using a Caputo time-fractional Maxwell model. The flow is a typical rectangular channel flow. When the scale of the cross-stream is much smaller than the streamwise and spanwise scales, the model is approximated as a two-dimensional slit parallel plate flow. Moreover, the influence of the electric double layer (EDL) at the solid–liquid interface is also considered. The electro-osmotic force generated by the interaction between the electric field and the EDL will induce a flow (i.e., electro-osmotic flow). Due to the application of the electric field at the streamwise and the vertical magnetic field, the flow is driven by Lorentz force along the spanwise direction. Simultaneously, under the action of the magnetic field, the electro-osmotic flow induces a reverse Lorentz force, which inhibits the electro-osmotic flow. The result shows that resonance behavior can be found in both directions in which the flow is generated. However, compared with the classical Maxwell fluid, the slip velocity and resonance behavior of fractional Maxwell fluid are suppressed. In the spanwise direction, increasing the strength of magnetic field first promotes the slip velocity and resonance behavior, and then suppresses them, while in the streamwise direction, both the electro-osmotic flow and resonance behavior are suppressed with the magnetic field. Full article

This paper aims to develop a review of the electrokinetic flow in microchannels. Thermal characteristics of electrokinetic phenomena in microchannels based on the Poisson–Boltzmann equation are presented rigorously by considering the Debye–Hückel approximation at a low zeta potential. Several researchers developed new mathematical models for high electrical potential with the electrical double layer (EDL). A literature survey was conducted to determine the velocity, temperature, Nusselt number, and volumetric flow rate by several analytical, numerical, and combinations along with different parameters. The momentum and energy equations govern these parameters with the influences of electric, magnetic, or both fields at various preconditions. The primary focus of this study is to summarize the literature rigorously on outcomes of electrokinetically driven flow in microchannels from the beginning to the present. The possible future scope of work highlights developing new mathematical analyses. This study also discusses the heat transport behavior of the electroosmotically driven flow in microchannels in view of no-slip, first-order slip, and second-order slip at the boundaries for the velocity distribution and no-jump, first-order thermal-slip, and second-order thermal-slip for the thermal response under maintaining a uniform wall-heat flux. Appropriate conditions are conferred elaborately to determine the velocity, temperature, and heat transport in the microchannel flow with the imposition of the pressure, electric, and magnetic forces. The effects of heat transfer on viscous dissipation, Joule heating, and thermal radiation envisage an advanced study for the fluid flow in microchannels. Finally, analytical steps highlighting different design aspects would help better understand the microchannel flow’s essential fundamentals in a single document. They enhance the knowledge of forthcoming developmental issues to promote the needed study area. Full article

Duchenne muscular dystrophy (DMD) is a degenerative genetic myopathy characterized by complete absence of dystrophin. Although the mdx mouse lacks dystrophin, its phenotype is milder compared to DMD patients. The incorporation of a null mutation in the Cmah gene led to a more DMD-like phenotype (i.e., more fibrosis). Although fibrosis is thought to be the major determinant of ‘structural weakness’, intracellular remodeling of myofibrillar geometry was shown to be a major cellular determinant thereof. To dissect the respective contribution to muscle weakness, we assessed biomechanics and extra- and intracellular architecture of whole muscle and single fibers from extensor digitorum longus (EDL) and diaphragm. Despite increased collagen contents in both muscles, passive stiffness in mdx Cmah${}^{-/-}$ diaphragm was similar to wt mice (EDL muscles were twice as stiff). Isometric twitch and tetanic stresses were 50% reduced in mdx Cmah${}^{-/-}$ diaphragm (15% in EDL). Myofibrillar architecture was severely compromised in mdx Cmah${}^{-/-}$ single fibers of both muscle types, but more pronounced in diaphragm. Our results show that the mdx Cmah${}^{-/-}$ genotype reproduces DMD-like fibrosis but is not associated with changes in passive visco-elastic muscle stiffness. Furthermore, detriments in active isometric force are compatible with the pronounced myofibrillar disarray of the dystrophic background. Full article

Calculation of the electrostatic double layer force (EDL force) between clay particles is relevant as it is closely related to important macroscopic mechanical behaviors of clays. The popular method to calculate the EDL force is to integrate the electric potential and Maxwell stress along the boundary enclosing a simply connected domain within which a clay particle resides. The EDL force has also been calculated by the integration of the electrostatic force density over the preceding domain. However, the subtle relation of the EDL forces calculated by the different existing methods has not yet been investigated. By means of theoretical analysis and finite element simulation, it was shown that the force calculated by the integration of Maxwell stress along the complete boundary enclosing a multiply connected domain in which the clay particle is excluded, and that along the partial boundary enclosing the preceding simply connected domain represents the electrical attractive force and osmotic repulsive force, respectively, while the integration of the potential along both the same complete and partial boundary denotes the osmotic force. Numerical results showed that the calculated EDL force deviates from its actual value significantly with the decrease in distance between the chosen integral boundary and particle surface, and the deviation varies with surface potential and angle between particles. Moreover, the recommended minimum distance was proposed to be 10 times the thickness of the particle based on the present simulation results. Full article