Search Results (195)

The genus Hippeastrum (Amaryllidaceae) is very popular worldwide for its attractive, colourful flowers. The aim of the research was to evaluate the possibility of replacing synthetic growth regulators added to the media with biostimulants of natural origin on the micropropagation and acclimatisation efficiency of several Hippeastrum genotypes. The effect of the biostimulants on the starch and fructan content of the bulbils after a 10-week in vitro culture was also investigated. The addition of biostimulant Goteo to the MS medium in vitro resulted in increased plantlet weight, leaf length and root number for most of the genotypes tested. The Folium biostimulant, not previously used in in vitro plant culture media showed positive effects, especially on leaf length. The use of Goteo during plant acclimatisation significantly improved root growth parameters, indicating its potential in minimising stress associated with the change from in vitro to ex vitro conditions. Higher starch content was detected in the bulbils of clone 0017-01 and the highest fructan content in the bulbils of clone 18 H. × chmielii. The use of the Folium biostimulant, consisting mainly of amino acids, reduced sugar accumulation, which may have been due to a shift in carbon allocation from storage processes to photosynthesis. The results confirm that biostimulants based on natural ingredients can be an alternative to synthetic growth regulators in the micropropagation of Hippeastrum and possibly other ornamental plants, what will be an input to further development of sustainable horticulture production. Full article

In agriculture, the use of fructans has gained relevance due to their ability to improve plant immunity and resistance to pathogens. However, many studies use high-purity fructans, which makes their application more expensive. In this work, the efficacy of two agave fructans, one food grade from Agave tequilana Weber var. Azul (FT) and the other obtained by semi-craft extraction from A. cupreata (FC) were evaluated in comparison with reagent-grade inulin from dahlia tubers. The effectiveness of their defense response against Phytophthora capsici infection in tomato (Solanum lycopersicum L.) was analyzed by evaluating defense mechanisms, including lignin deposition, hydrogen peroxide (H₂O₂) accumulation, and β-1,3-glucanase and peroxidase activity. The results indicated that foliar application of both fructans showed protection against infection, reducing disease incidence and severity. FT fructans at lower concentration (0.5 g/L) showed the highest protection, followed by FC, while dahlia inulin showed lower effectiveness. An early and progressive accumulation of H₂O₂ was observed in fructan-treated plants, in contrast to the late increase in untreated infected plants. Also, peroxidase activity was higher in the fructan treatments, suggesting a more efficient defense response. Although lignin deposition was not directly correlated with protection against P. capsici, fructans showed potential as resistance inducers. Given their low cost, easy extraction, and zero environmental impact, agave fructans represent a viable alternative for crop protection in sustainable agricultural systems. This study opens the door to their validation in the field and their application in other economically important crops, contributing to biological control strategies with less dependence on agrochemicals. Full article

Fructooligosaccharides (FOS) are short fructans with different degrees of polymerization (DP) and bonds in their structure, generated by the distinct activities of fructosyltransferase enzymes, which produce distinct types of links. FOS are in high demand on the market, mainly because of their prebiotic effects. In recent years, depending on the link type in the FOS structure, prebiotic activity has been shown to be increased. Studies on β-fructanofuranosidases (Ffasa), enzymes with fructosyltransferase activity in yeasts, have reported the production of ¹F-FOS, ⁶F-FOS, and ⁶G-FOS. The aims of this investigation were to evaluate the capability of fifteen different yeasts to grow in Agave sp. juices and to determine the potential of these juices as substrates for FOS production. Additionally, the research aimed to corroborate and analyze the fructosyltransferase activity of enzymatic extracts obtained from agave yeasts by distinct induction media and to identify the role and optimal parameters (time and sucrose and glucose concentrations) for FOS and disaccharides production through Box–Behnken designs. To carry out such a task, different techniques were employed: FT-IR, TLC, and HPAEC-PAD. We found two yeasts with fructosyltransferase activity, P. kudriavzevii ITMLB97 and C. lusitaniae ITMLB85. In addition, within the most relevant results, the production of the FOS 1-kestose, 6-kestose, and neokestose, as well as disaccharides inulobiose, levanobiose, and blastose, molecules with potential applications, was determined. Overall, FOS production requires suitable yeast species, which grow in a medium under optimal conditions, from which microbial enzymes with industrial potential can be obtained. Full article

Irritable bowel syndrome (IBS) is a gut–brain axis chronic disorder, characterized by recurrent abdominal pain and altered bowel habits in the absence of organic pathology. Nutrition plays a central role in symptom management, yet no single dietary strategy has demonstrated universal effectiveness. This narrative review critically evaluates current nutritional approaches to IBS. The low-Fermentable Oligo-, Di-, Mono-saccharides and Polyols (FODMAP) diet is the most extensively studied and provides short-term symptom relief, but its long-term effects on microbiota diversity remain concerning. The Mediterranean diet, due to its anti-inflammatory and prebiotic properties, offers a sustainable, microbiota-friendly option; however, it has specific limitations in the context of IBS, particularly due to the adverse effects of certain FODMAP-rich foods. A gluten-free diet may benefit individuals with suspected non-celiac gluten sensitivity, although improvements are often attributed to fructan restriction and placebo and nocebo effects. Lactose-free diets are effective in patients with documented lactose intolerance, while a high-soluble-fiber diet is beneficial for constipation-predominant IBS. IgG-based elimination diets are emerging but remain controversial and require further validation. In this review, we present the 10 dietary commandments for IBS, pragmatic and easily retained recommendations. It advocates a personalized, flexible, and multidisciplinary management approach, avoiding rigidity and standardized protocols, with the aim of optimizing adherence, symptom mitigation, and health-related quality of life. Future research should aim to evaluate, in real-world clinical settings, the impact and applicability of the 10 dietary commandments for IBS in terms of symptom improvement and quality of life Full article

Barley, rich in beneficial ingredients, has been recognised as a healthy food and is widely used in the production of healthy foods for humans. The current study identified a new barley mutant with the SSIIa, SSIIIa, SBEIIa, and SBEIIb genes inactivated in the genome-edited offspring of targeted mutagenesis of starch synthetic genes using multiplex genome editing. The grain compositions and starch properties of the ssIIa/ssIIIa/sbeIIa/sbeIIb mutant were analysed and compared with the corresponding parameters of ssIIa, ssIIIa, sbeIIa/sbeIIb, ssIIa/sbeIIa/sbeIIb, and non-genome-edited lines (NE), respectively. ssIIa/ssIIIa/sbeIIa/sbeIIb exhibited the highest contents of β-glucan and amylose content among all mutants and NE, but not the most prominent in resistant starch, fructan, and fibre contents. The loss of SSIIa, SSIIIa, SBEIIa, and SBEIIb genes also resulted in significant changes in starch properties. This study enriched the genotypes of healthy barley and provided a theoretical basis for improving barley quality. Full article

Inulin and agavin fructans have been widely used in the food industry as fat substitutes, wall materials, and prebiotics, among other applications. Chemical modifications offer several advantages, from enhancing functional properties to broadening industrial applications, making them a key area of research in biotechnology, nutrition, and food science. This review examines the chemical modifications of fructans, specifically the inulin and agavin types. It describes the most commonly used methods, their characteristics, and their impact on the physicochemical, functional, and prebiotic properties of fructans. Additionally, it explores the interactions underlying these changes. Modifications enhance, extend, or generate new biological properties and activities. While most yield positive outcomes, challenges remain, including a deeper understanding of the structure–bioactivity relationships and further toxicity assessments, particularly in agavins. These insights aim to guide future research and innovation in the field. Full article

This study aimed to develop innovative fruit leather with programmed health-promoting properties, enriched with fructooligosaccharides (FOS) from chicory and Jerusalem artichoke. Their physicochemical properties were assessed, including the profile of polyphenolic compounds, pro-health effects, and sensory characteristics. The products contained various fruits (including pear, red currant, peach, and haskap berry) and 10% FOS powders. It was shown that the addition of FOS reduced acidity and total sugar content while increasing fiber content—especially fructans—and selected minerals (K, Mg, Zn). The addition of FOS also modulated the profile of polyphenolic compounds, whereas fruit leather without FOS was characterized by a higher concentration of these compounds. It was shown that the addition of chicory significantly modulates the ability to inhibit α-glucosidase. At the same time, in the case of the Jerusalem artichoke, the inhibition efficiency depends on the type of fruit matrix. Sensory-wise, the highest scores were given to recipes without FOS additives, with Jerusalem artichoke being better accepted than chicory. The results indicate the potential of using FOS as a functional additive, but their effects on taste and texture require further optimization. Full article

The current study aims for the isolation and physicochemical characterization of inulin from defatted dandelion roots using green extraction techniques, including microwave extraction (MAE) and ultrasound-assisted extraction (UAE). The structure and degree of polymerization of inulin were elucidated by chromatographic techniques, as well as by FTIR and NMR spectroscopies. The color characteristics, water- and oil-holding capacity, solubility, swelling properties, wettability, angle of repose, flowability, and cohesiveness of dandelion inulin were evaluated. Moreover, the antioxidant and antibacterial potential of dandelion inulin were revealed. The results were compared with the conventional extraction and inulin from chicory. Dandelion inulin was evaluated as a powder substance with a degree of polymerization (DP) of 17–24. The highest yield (20%) was obtained by classical extraction; however, UAE and MAE demonstrated the highest purity. FT-IR and NMR spectra revealed that dandelion inulin is glucofructan with a molecular weight of 2.7–3.2 kDa that consists mainly of fructosyl units β-(2→1) linked to one α-D-glucose unit UAE was evaluated as the most perspective technique for the simultaneous extraction of inulin from dandelion roots, with the highest average DP 24 and high purity (82%), molecular mass, total fructose content, swelling index, and oil-holding capacity. Dandelion inulin exhibited intermediate cohesiveness, fair flowability, and moderate antimicrobial activity against Listeria monocytogenes 863 and Bacillus subtilis 6633. The physicochemical and functional properties of dandelion inulin reveal its future potential as an additive in food, cosmetic, and pharmaceutics formulations as a texture modifier, a fat replacer, and a drug carrier. Full article

Exopolysaccharides (EPSs) produced by probiotic bacteria have garnered attention due to their effects on the gut health of humans and animals. The nutrients that probiotics access during their growth are essential for producing beneficial effects on host health. Direct immunomodulatory effects of graminan-type fructans (GTFs) from Agave tequilana through toll-like receptors (TLRs) have been demonstrated. However, the immunomodulatory effects of these fructans, mediated through the EPSs produced by the probiotics cultivated with them, remain unexplored. We explored the immunomodulatory effects of lactic acid bacteria (LAB) strains isolated from swine and their EPSs, based on the GTFs used as carbon sources during their growth. While the LAB strains activated the NF-κB pathway independently of the GTF source, their EPSs activated it in a GTF source-dependent manner. LAB activation through TLR2 showed a GTF source dependency, whereas their EPSs activated TLR2 independently of the GTF source. The LAB and their EPSs activated TLR4 in a GTF source-dependent manner. Both the LAB and their EPSs inhibited the activation of TLR2 and TLR4 agonists, which exhibited a strong dependence on the GTF source. The strength of GTF C’s immunomodulatory effects on LAB illustrates its specificity, its impact on the EPS structure, and its biological effects. Our results support the promising health benefits of this synbiotic model for swine health and lowering inflammation. Full article

FOSs are short-chain fructose-based oligosaccharides with notable functional and health benefits. Naturally present in various fruits and vegetables, FOSs are primarily produced enzymatically or microbially from sucrose or long-chain fructans, namely, inulin. Enzymes such as fructosyltransferase, β-fructofuranosidase, and endoinulinase are typically involved in its production. The chemical structure of FOSs consists of an assembly of fructose residues combined with a glucose unit. The increasing consumer demand for healthy foods has driven the widespread use of FOSs in the functional food industry. Thus, FOSs have been incorporated into dairy products, beverages, snacks, and pet foods. Beyond food and feed applications, FOSs serve as a low-calorie sweetener for and are used in dietary supplements and pharmaceuticals. As a prebiotic, they enhance gut health by promoting the growth of beneficial bacteria, aid digestion, improve mineral absorption, and help regulate cholesterol and triglyceride levels. Generally recognized as safe (GRAS) and approved by global regulatory agencies, FOSs are a valuable ingredient for both food and health applications. This review provides an updated perspective on the natural sources and occurrence of FOSs, their structures, and physicochemical and physiological features, with some focus on and a critical assessment of their potential health benefits. Moreover, FOS production methods are concisely addressed, and forthcoming developments involving FOSs are suggested. Full article

Lettuce is a globally cultivated and consumed leafy crop. Here we developed an efficient tobacco rattle virus (TRV)-based guide RNA (gRNA) delivery system for CRISPR/Cas editing in the commercial lettuce cultivar ‘Noga’. Plants stably expressing Cas9 were inoculated with TRV vectors carrying gRNAs targeting five nutrient-associated genes. The system achieved an average editing efficiency of 48.7%, with up to 78.9% of regenerated plantlets showing independent mutations. This approach eliminates the need for antibiotic selection, simplifying tissue culture processes. The system supports diverse applications, including Cas12a editing and large-fragment deletions using dual gRNA sets. Targeting the fructan 1-exohydrolase 2 (1-FEH2) gene produced knockout lines with significant increases in prebiotic dietary fibre fructan content, up to 5.2-fold, and an average rise in the degree of polymerisation by 2.15 units compared with controls. Combining 1-FEH1 and 1-FEH2 knockouts did not further increase fructan levels, revealing 1-FEH2 as the predominant isozyme in lettuce. RT-qPCR analysis showed reduced expression of the upstream biosynthetic enzyme sucrose:sucrose 1-fructosyl transferase (1-SST), suggesting potential feedback inhibition in fructan metabolism. This TRV-based gene editing approach, utilised here to increase fructan content, could be applied to improve other valuable traits in lettuce, and may inspire similar systems to enhance nutritional content of crops. Full article

This study investigates the effect of extended rye scald fermentation times (12–48 h) on its biochemical properties and rye bread’s nutritional and sensory qualities. Traditional rye bread production in Latvia involves prolonged fermentation with lactic acid bacteria (LAB), a process that influences the bread’s acidity, sugar content, and concentrations of organic acids, fructans, and phytates. Scald fermentation was analyzed at intervals of 0, 12, 24, 36, and 48 h to monitor microbial activity, particularly LAB population dynamics. Organic acids and sugar profiles were analyzed using HPLC, while phytic acid and fructan concentrations were determined using the Phytic Acid Assay Kit (K-PHYT) and Fructan Assay Kit (K-FRUC). Sensory evaluation assessed attributes including aroma, sour and sweet taste, stickiness, and floury aftertaste. A rapid pH decrease and increased total titratable acidity (TTA) after 12 h confirmed scald’s suitability as a substrate for Lactobacillus delbrueckii metabolism. Lactic acid content increased 13.8-fold after 48 h. Combined scald and dough sourdough fermentation reduced phytic acid by 20% and fructans by 49%, improving mineral bioavailability. Extending fermentation beyond 24 h showed no significant differences in physicochemical parameters, although it improved sensory quality, reduced stickiness, balanced sweet–sour flavors, enhanced aroma, and minimized floury aftertaste. Full article

Levansucrases are key enzymes responsible for the synthesis of β-2,6-linked fructans, found in plants and microbes, especially in bacteria. Levansucrases have been applied in the production of levan biopolymer and fructooligosaccharides (FOSs) using sucrose as a substrate as well as in reducing sugar levels in fruit juice. As a result, levansucrases that are active at low temperatures are required for industrial applications to maintain product stability. Therefore, this work firstly reports the novel cold-active levansucrase (SacBPk) isolated from a sucrolytic bacterial strain, P. koreensis HL12. The SacBPk was classified into glycoside hydrolase family 68 subfamily 1 (GH68_1) and comprised a single catalytic domain with the Asp104/Asp267/Glu362 catalytic triad. Interestingly, the recombinant SacBPk demonstrated cold-active levansucrase activity at low temperatures (on ice and 4–40 °C) with the highest specific activity (167.46 U/mg protein) observed at 35 and 40 °C in 50 mM sodium phosphate buffer pH 6.0. SacBPk mainly synthesized levan polymer as the major product (129 g/L, corresponding to 25.8% of total sugar) with a low number of short-chain FOSs (GF2–4) (12.8 g/L, equivalent to 2.5% of total sugar) from 500 g/L sucrose after incubating at 35 °C for 48 h. These results demonstrate the industrial application potential of SacBPk levansucrase for levan and FOSs production. Full article

Jerusalem artichoke (Helianthus tuberosus L.) reproduces asexually through tubers, which are rich in fructan-type carbohydrates and serve as the primary processing organ. Plant hormones may regulate the sprouting process of tubers, but the changes in fructans and their regulatory mechanisms in relation to hormones remain unclear. This study utilized two varieties of Jerusalem artichoke, “Qingyu No.1” and “Qingyu No.3”, to analyze the changes in the proportion of carbohydrates (especially fructans) in total sugars during the sprouting process. Metabolomic and transcriptomic analyses were conducted at three selected sprouting stages. The results indicated that during tuber sprouting, carbohydrates such as fructans play a regulatory role through conversion activities. Multi-omics analysis revealed that jasmonic acid and salicylic acid promote Jerusalem artichoke sprouting through metabolism and are involved in the plant hormone signal transduction process. Differentially expressed genes related to hormone signaling were screened and divided into three groups based on expression levels. It was found that the proportion of carbohydrates is highly correlated with the expression of hormone-related genes in the sprouts, suggesting that plant hormones may regulate carbohydrate metabolism during the sprouting of Jerusalem artichoke tubers. In conclusion, these results preliminarily elucidate the regulatory mechanisms of plant hormones in Jerusalem artichoke tuber sprouting, aiming to provide a theoretical basis for the production and processing of Jerusalem artichoke. Full article

Pasta is a staple food consumed worldwide and is made from wholegrain semolina, which is a food rich in dietary fibre, proteins, minerals, vitamins, and bioactive compounds. However, fermentable oligo-, di-, and monosaccharides and polyols (FODMAP), part of soluble dietary fibre in pasta, can trigger/worsen irritable bowel syndrome (IBS) symptoms and increase the prevalence of gastrointestinal disorders. These dietary fibres include lactose, excess fructose relative to glucose, polyols, fructans (mostly fructooligosaccharides), and galactooligosaccharides. Due to a lack of information on the FODMAP profile for pasta, this research conducted a detailed analysis using high-performance anion-exchange chromatography with pulsed amperometric detection to determine the FODMAP compound content in commercially available pasta, with a focus on wholegrain products. The results showed that fructooligosaccharides (FOSs) are the dominant group of FODMAPs, and kestose is the predominant oligosaccharide in all pasta samples both dry (67.1–95.0%) and cooked (27.1–93.9%). Almost all pasta samples are classified as high-FODMAP foods. The degree of reduction in FODMAP compound content during cooking varies between pasta types and is influenced by the wheat type, cooking time, amount of water used for cooking, pasta shapes, and pasta supplementation. In samples of dry pasta, there are statistically significant differences in the results between all samples, while after cooking, there is evident grouping of the results in four clusters. The reduction in FOS content of pasta after cooking was in the range from 30.9% to 84%. Further research should be focused on higher activity of FODMAP degrading enzymes during pasta production process. Full article