Search Results (5,334)

Hoarseness is a common voice symptom that can impair communication and lead to psychosocial difficulties. It has been hypothesized that chronic hoarseness may contribute to elevated social anxiety. This study aimed to assess the impact of hoarseness on quality of life and social anxiety in affected individuals. Thirty-eight patients with chronic hoarseness (voice disorders) and 40 matched healthy controls were evaluated in a prospective case-control study. Quality of life was measured using the WHOQOL-BREF questionnaire (Physical, Psychological, Social, and Environmental domains). Social anxiety was assessed with the Liebowitz Social Anxiety Scale (LSAS), and general anxiety and depression with the Hospital Anxiety and Depression Scale (HADS). Group scores were compared using appropriate statistical tests, and effect sizes with 95% confidence intervals were calculated. Patients with hoarseness had significantly lower Psychological Health and Social Relationships scores on the WHOQOL-BREF than controls (p < 0.01 for both; large effect sizes), indicating worse quality of life in these domains. Physical Health and Environmental domain scores did not differ between groups. The hoarseness group also showed higher social anxiety: LSAS total scores and Social Interaction subscale scores were significantly greater than those of controls (p < 0.01 and p < 0.05, respectively; moderate-to-large effects), whereas the Performance Anxiety subscale was similar between groups. By contrast, HADS anxiety and depression scores did not differ significantly between patients and controls. Notably, mean HADS scores in both groups fell in the mild (borderline) range rather than the normal range. Chronic hoarseness is associated with reduced quality of life in emotional and social domains and with increased social anxiety symptoms, but not with elevated general anxiety or depression. These findings underscore the need to address psychosocial factors, particularly social anxiety, in the clinical management of patients with voice disorders. Full article

Background/Objectives: Fear of missing out (FOMO) refers to the pervasive experience of worrying that others may be having rewarding or meaningful experiences from which one is absent or excluded. FOMO has been linked with both sleep disturbances and poor mental health outcomes, particularly in emerging adults (ages 18–29). This study tested whether insomnia symptoms mediate the relationship between FOMO and depressive symptoms in emerging adults and whether gender moderates the links between FOMO, insomnia symptoms, and depression symptoms. Methods: We conducted a secondary analysis of cross-sectional survey data from 849 emerging adults in the United States. Participants completed validated measures of FOMO, insomnia symptoms, and depression symptoms. We tested our hypotheses using regression models in SPSS version 29 and mediation and moderation models via the PROCESS macro. Analyses included age, race/ethnicity, and education as covariates. Results: FOMO predicted greater insomnia severity and more depression symptoms, and insomnia severity partially mediated the link between FOMO and depression symptoms. The FOMO–insomnia association was moderated by gender, with a stronger link among men. Conclusions: These findings suggest that insomnia is a plausible mechanism linking FOMO to depression in emerging adults. Gender differences suggest that FOMO may disproportionately disrupt sleep in men and highlight the need for tailored prevention efforts to target both FOMO and sleep disruption among emerging adults. Full article

Background/Objectives: All-inside ACL reconstruction has emerged as a minimally invasive alternative to conventional techniques, with potential advantages in graft configuration and reduced surgical trauma. This study aimed to compare the clinical outcomes of all-inside and full tibial tunnel ACL reconstruction, focusing on graft diameter, postoperative stability, and functional recovery. We hypothesized that the all-inside technique would allow for thicker grafts and yield superior postoperative knee stability and functional outcomes, with postoperative anterior laxity as a major outcome of interest. Methods: This retrospective comparative study reviewed patients who underwent ACL reconstruction between January 2020 and February 2024. From January 2020 to September 2022, a four-strand hamstring autograft with full tibial tunnel technique (FT-4) was used, while from September 2022, a six-strand hamstring autograft with the all-inside technique (AI-6) was adopted to enable thicker grafts and optimize fixation. Among a total of 103 patients, 1:1 propensity score matching (PSM) was performed based on age, sex, BMI, laterality, ALL reconstruction, meniscal lesion, and preoperative anterior laxity (SSD). Graft diameter and clinical outcomes, including knee stability and functional scores, were compared between the matched groups. Results: After PSM, two comparable groups of 29 patients each were established. Graft diameter was significantly larger in the AI-6 group (9.5 ± 0.7 mm) compared to the FT-4 group (7.8 ± 0.8 mm, p < 0.001), while other baseline characteristics remained well balanced between the groups. At the final follow-up, both groups exhibited significant improvements in anterior laxity, functional scores, and pivot shift grades (all p < 0.001). The AI-6 group demonstrated superior outcomes with a significantly higher Lysholm score (82.2 ± 6.7 vs. 75.6 ± 8.9, p = 0.002), lower WOMAC score (8.0 ± 4.3 vs. 12.9 ± 10.5, p = 0.023), and reduced anterior laxity (1.6 ± 1.1 mm vs. 2.5 ± 1.4 mm, p = 0.005) compared to the FT-4 group, whereas no significant differences were observed in the IKDC, Tegner, Korean knee score, or pivot shift test results. A simple linear regression revealed a significant negative correlation between graft diameter and postoperative anterior laxity (B = −0.398, p = 0.048). Conclusions: The present study demonstrated that the use of a six-strand hamstring graft configuration in the AI-6 technique resulted in significantly thicker grafts and was associated with reduced postoperative anterior knee laxity compared to the FT-4 technique. While interpretation of these findings requires caution in light of MCID thresholds, the AI-6 group showed favorable outcomes in anterior laxity and selected functional scores, such as the Lysholm and WOMAC. This technique may offer practical clinical value, particularly in populations prone to smaller graft diameters, as it facilitates adequate graft thickness through multifold preparation, with the all-inside approach accommodating the inherent graft shortening. Full article

Background and purpose: Vulvovaginal candidiasis (VVC), caused by Candida albicans (C. albicans), is exacerbated by oxidative stress and uncontrolled inflammation. Pathogens like C. albicans generate reactive oxygen species (ROS) to enhance virulence, while host immune responses further amplify oxidative damage. This study investigates the antioxidant and antifungal properties of Hyssopus cuspidatus Boriss volatile extract (SXC), a traditional Uyghur medicinal herb, against fluconazole-resistant VVC. We hypothesize that SXC’s bioactive volatiles counteract pathogen-induced oxidative stress while inhibiting fungal growth and inflammation. Methods: GC-MS identified SXC’s major bioactive components, while broth microdilution assays determined minimum inhibitory concentrations (MICs) against bacterial/fungal pathogens, and synergistic interactions with amphotericin B (AmB) or fluconazole (FLC) were assessed via time–kill kinetics. Anti-biofilm activity was quantified using crystal violet/XTT assays, and in vitro studies evaluated SXC’s effects on C. albicans-induced cytotoxicity (LDH release in A431 cells) and inflammatory responses (cytokine production in LPS-stimulated RAW264.7 macrophages). A murine VVC model, employing estrogen-mediated pathogenesis and intravaginal C. albicans challenge, confirmed SXC’s in vivo effects. Immune modulation was assessed using ELISA and RT-qPCR targeting inflammatory and antioxidative stress mediators, while UPLC-MS was employed to profile metabolic perturbations in C. albicans. Results: Gas chromatography-mass spectrometry identified 10 key volatile components contributing to SXC’s activity. SXC exhibited broad-spectrum antimicrobial activity with MIC values ranging from 0.125–16 μL/mL against bacterial and fungal pathogens, including fluconazole-resistant Candida strains. Time–kill assays revealed that combinations of AmB-SXC and FLC-SXC achieved sustained synergistic bactericidal activity across all tested strains. Mechanistic studies revealed SXC’s dual antifungal actions: inhibition of C. albicans hyphal development and biofilm formation through downregulation of the Ras1-cAMP-Efg1 signaling pathway, and attenuation of riboflavin-mediated energy metabolism crucial for fungal proliferation. In the VVC model, SXC reduced vaginal fungal burden, alleviated clinical symptoms, and preserved vaginal epithelial integrity. Mechanistically, SXC modulated host immune responses by suppressing oxidative stress and pyroptosis through TLR4/NF-κB/NLRP3 pathway inhibition, evidenced by reduced caspase-1 activation and decreased pro-inflammatory cytokines (IL-1β, IL-6, TNF-α). Conclusions: SXC shows promise as a broad-spectrum natural antimicrobial against fungal pathogens. It inhibited C. albicans hyphal growth, adhesion, biofilm formation, and invasion in vitro, while reducing oxidative and preserving vaginal mucosal integrity in vivo. By disrupting fungal metabolic pathways and modulating host immune responses, SXC offers a novel approach to treating recurrent, drug-resistant VVC. Full article

Isocitrate dehydrogenase (IDH) catalyzes the conversion of NAD(P)⁺ and isocitrate to NAD(P)H and α-ketoglutarate (αKG). The cytoplasmic enzyme IDH1 is important for producing NADPH for biosynthesis and for protecting against oxidative stress. IDH1 mutants, such as R132H found in glioblastomas and other types of human cancers, have a neomorphic activity that uses NADPH to reduce αKG to 2-hydroxyglutarate (2HG). 2HG interferes with the activity of important enzymes such as histone demethylases and TET demethylases. We hypothesized that Caenorhabditis elegans could be a good model system for studying oncogenic properties of mutant IDH1. To test this, we purified C. elegans cytoplasmic IDH-1 and two mutants, G98N and R133H, which correspond to human IDH1 mutants G97N and R132H, respectively. We found that the wild-type IDH-1 had similar kinetic properties to human IDH1, and it could produce small amounts of 2HG. We also found that the R133H mutant had a lower K_M for αKG than human R132H in steady-state enzyme kinetic experiments, and it produced almost exclusively 2HG in the presence of NADPH and αKG. The G98N mutant had a higher k_cat in the forward direction than the comparable human G97N mutant, and the G98N mutant produced a smaller amount of 2HG compared to the R133H mutant. These results suggest that C. elegans strains with IDH-1 mutations could be a good model system for studying the effects of 2HG in eukaryotic organisms. Full article

Locus of control refers to the way in which people perceive whether they have control over situations in their lives or whether these situations are the result of external circumstances. Locus of control subsequently influences individuals’ motivation, decision-making, and ability to accept responsibility. How locus of control manifests itself in the behavior of a particular individual can be influenced by several factors. In this article, we focused on how elements of different dimensions of human resource diversity can influence locus of control. For the research, we chose a quantitative approach using a questionnaire measuring the locus of control, along with additional questions. The main aim of the presented research was to identify the relationship between sociodemographic variables and the locus of control orientation of individual groups of respondents. The research sample consisted of N = 384 participants who completed the reduced standardized Rotter locus of control scale. The results focused on differences in individuals’ locus of control in terms of age, gender, type of work experience, and marital status and to what extent these sociodemographic variables can be a predictor of individuals’ locus of control. Hypotheses testing was performed using IBM SPSS 23 software. Th theoretical application of the research findings lies in the discovery that the locus of control (LoC) is not influenced by simple characteristics but must be understood in a more complex way. The practical application lies in the fact that professional experience can influence how employees perceive their level of control over their ability to affect their work and outcomes. Full article

Business models often transform due to adaptation in response to external changes. However, relatively little is known about what causes these types of adaptations. We suggest that threat-rigidity as well as prospect theory have the potential to explain what causes business model adaptation in response to gains and losses. Firm leaders’ inclination to adapt their business model is sensitive to risk that is perceived as a gain or a loss in the macro-economic environment. We apply threat-rigidity and prospect theories to examine the relationship between risk perception and business model adaptation. We also investigate if emotion has explanatory value for how managers adapt to business models. We test our hypotheses in a field experiment involving 95 Scandinavian managers. Here, we relate managers’ inclinations to adapt to different business models under different risk scenarios. The results reveal that, in general, managers are more risk seeking in gain scenarios than in loss scenarios. This finding is in line with the threat-rigidity theory. In addition, emotional style is found to relate more to risk aversion than to risk seeking in the domain of potential gain. We argue that emotional style has explanatory value for how managers adapt to business models, because emotions are key influencers on risk perception. Full article

As environmental conservation and community development gain importance, ecotourism has emerged as a significant segment of the global tourism industry. However, the cultural factors that drive tourist behavior in this domain remain underexplored. This research examined how power distance belief (PDB), interacts with the type of tourism content shared on social media (conspicuous versus experiential) to influence travelers’ ecotourism intentions. To test our hypotheses, we conducted two experimental studies using a 2 (PDB: high vs. low) × 2 (tourism content type: conspicuous vs. experiential) between-subjects design. Participants for both experiments (N = 480) were recruited through an online survey platform. In the experiments, participants’ PDB was situationally primed, and tourism content type was manipulated using specifically created fictitious posts adapted from a real social media platform. Other key variables were measured using validated multi-item scales. Data were analyzed using analysis of variance (ANOVA) and moderated mediation analysis (PROCESS Model 15). The findings reveal that travelers with high PDB show higher ecotourism intentions when exposed to conspicuous content, whereas travelers with low PDB exhibit higher intentions when exposed to experiential content. This interactive effect is mediated by travelers’ social comparison motives. These findings offer novel insights into the motivations underlying ecotourism behavior by identifying distinct pathways through which social media can promote sustainable tourism behaviors, and provide practical guidance for eco-destination managers to design targeted marketing strategies that encourage sustainable tourism practices across different consumer segments. Full article

The global outbreak of the monkeypox virus was declared a health emergency by the World Health Organization (WHO). During such emergencies, misinformation about health suggestions can spread rapidly, leading to serious consequences. This study investigates the relationships between tweet readability, user engagement, and susceptibility to misinformation. Our conceptual model posits that tweet readability influences user engagement, which in turn affects the spread of misinformation. Specifically, we hypothesize that tweets with higher readability and grammatical correctness garner more user engagement and that misinformation tweets tend to be less readable than accurate information tweets. To test these hypotheses, we collected over 1.4 million tweets related to monkeypox discussions on X (formerly Twitter) and trained a semi-supervised learning classifier to categorize them as misinformation or not-misinformation. We analyzed the readability and grammar levels of these tweets using established metrics. Our findings indicate that readability and grammatical correctness significantly boost user engagement with accurate information, thereby enhancing its dissemination. Conversely, misinformation tweets are generally less readable, which reduces their spread. This study contributes to the advancement of knowledge by elucidating the role of readability in combating misinformation. Practically, it suggests that improving the readability and grammatical correctness of accurate information can enhance user engagement and consequently mitigate the spread of misinformation during health emergencies. These insights offer valuable strategies for public health communication and social media platforms to more effectively address misinformation. Full article

Bacterial cell morphology might result from natural selection to gain a competitive advantage under environmentally stressful conditions such as nutrient limitation. In nutrient-limited conditions, a higher surface-to-volume ratio is crucial for cell survival because it allows for a more efficient exchange of nutrients and waste products. A bacterial strain YC6860^T isolated from the rhizosphere of rice (Oryza sativa L.) showed pleomorphic behavior with smooth cell morphology and wrinkled surface rods depending upon nutritional conditions. Based on scanning and transmission electron microscopy studies, we hypothesized that the surface-to-volume ratio of cells would increase with decreasing nutrient concentrations and tested this quantitatively. The transition from smooth to wrinkled cell surface morphology could be one of the adaptation strategies by which YC6860^T maximizes its ability to access available nutrients. To characterize the properties of the wrinkled strain, we performed taxonomic and phylogenetic analyses. 16S rRNA gene sequencing results showed that the strain represented a novel, deep-rooting lineage within the order Rhizobiales with the highest similarity of 94.2% to Pseudorhodoplanes sinuspersici RIPI 110^T. Whole-genome sequencing was also performed to characterize its genetic features. The low phylogenetic and genetic similarity is probably related to the wrinkled morphology of the strain. Therefore, we propose that the strain YC6860^T might belong to a new genus and species, named Rugositalea oryzae. In addition, taxonomic analysis showed that YC6860^T is Gram-negative, aerobic, and rod-shaped with regular surface wrinkles under nutrient-limiting conditions, resembling a delicate twist of fusilli, with groove depths of 48.8 ± 3.7 nm and spacing of 122.5 ± 16.9 nm. This unique cell structure with regular rugosity could be the first finding that has not been reported in the existing bacterial morphology. Full article

Background/Objectives: Children with Autism Spectrum Disorder (ASD) often display heightened sensitivity to simple auditory stimuli, but have difficulty discriminating and integrating multiple phonological features (segmental: consonants and vowels; suprasegmental: lexical tones) at the syllable level, which negatively impacts their communication. This study aims to investigate the neural basis of segmental, suprasegmental and combinatorial speech processing challenges in Mandarin-speaking children with ASD compared with typically developing (TD) peers. Methods: Thirty children with ASD and thirty TD peers will complete a multi-feature oddball paradigm to elicit auditory ERP during passive listening. Stimuli include syllables with single (e.g., vowel only), dual (e.g., vowel + tone), and triple (consonant + vowel + tone) phonological deviations. Neural responses will be analyzed using temporal principal component analysis (t-PCA) to isolate overlapping ERP components (early/late MMN), and representational similarity analysis (RSA) to assess group differences in neural representational structure across feature conditions. Expected Outcomes: We adopt a dual-framework approach to hypothesis generation. First, from a theory-driven perspective, we integrate three complementary models, Enhanced Perceptual Functioning (EPF), Weak Central Coherence (WCC), and the Neural Complexity Hypothesis (NCH), to account for auditory processing in ASD. Specifically, we hypothesize that ASD children will show enhanced or intact neural discriminatory responses to isolated segmental deviations (e.g., vowel), but attenuated or delayed responses to suprasegmental (e.g., tone) and multi-feature deviants, with the most severe disruptions occurring in complex, multi-feature conditions. Second, from an empirically grounded, data-driven perspective, we derive our central hypothesis directly from the mismatch negativity (MMN) literature, which suggests reduced MMN amplitudes (with the exception of vowel deviants) and prolonged latencies accompanied by a diminished left-hemisphere advantage across all speech feature types in ASD, with the most pronounced effects in complex, multi-feature conditions. Significance: By testing alternative hypotheses and predictions, this exploratory study will clarify the extent to which speech processing differences in ASD reflect cognitive biases (local vs. global, per EPF/WCC/NCH) versus speech-specific neurophysiological disruptions. Findings will advance our understanding of the sensory and integrative mechanisms underlying communication difficulties in ASD, particularly in tonal language contexts, and may inform the development of linguistically tailored interventions. Full article

(1) Background: It seems that (i) the most frequent and critical physical tasks during police service are fence jump, victim drag, and arrest suspect, and (ii) high fitness attributes are conducive to solving police physical tasks with higher success rates. By this, this study aims (i) to evaluate the reliability for assessing police physical tasks (PPTs) and (ii) to identify the fitness attributes that best explain the performance in carrying out PPT. We hypothesize that performance in PPT presents high reliability and that fitness attributes are significant predictors of performance in PPT. (2) Methods: A total of 76 cadets from the Portuguese Police Academy completed (i) three PPT (fence jump—FJ; victim drag—VD; and arrest suspect—AS) in two distinct sessions (T1 and T2), separated by a one-week interval (test–retest design), and subsequently, separated by a one-week interval, (ii) seven fitness tests (T3; cross-sectional design). (3) Results: It was observed that (i) the mean difference in performance (T2-T1) in FJ was 0.05 s (ICC = 0.88), in VD was −0.06 s (ICC = 0.92), and in the AS was −1.21 s (ICC = 0.81); (ii) male cadets were significantly faster, more agile, stronger, and more resistant than female cadets, and they were significantly faster at FJ and VD; (iii) in females, performance in the 30 m sprint tests, sit-ups, and horizontal jump are predictors of FJ, VD, and AS, respectively; and (iv) in males, performance in the horizontal jump and the 20 m shuttle run are predictors of FJ performance, while handgrip strength is a significant predictor in the VD. (4) Conclusions: This study showed that (i) the PPT evaluation protocol presents high reliability (ICC of 0.87, SE = 0.17), suggesting that it is a reliable protocol, capable of being applied to police officers, and (ii) within the scope of attributes that predict performance in the PPTs under study, it appears that the explosive strength of the lower limbs is a relevant attribute, regardless of gender. Full article

In the era of digital transformation and data-driven decision-making, big data analytics capability (BDAC) is crucial for firms to enhance innovation and sustainable competitive advantage in highly dynamic markets. Grounded in dynamic capability theory, this study used a moderated mediation model to explore the impact of BDAC on innovativeness. Empirical analysis was conducted by using survey data from 270 enterprises to test the hypotheses. The results reveal that BDAC significantly and positively influences innovativeness, and sustained innovation mediates this relationship. Moreover, organizational slack positively moderates the effect of BDAC on innovativeness, both the direct effect and indirect effect. These findings provide theoretical support and practical implications for understanding how BDAC enhances firm innovativeness. Full article

Huntington’s Disease (HD) originates from the expansion of a polyglutamine (PolyQ) tract in the huntingtin protein (Htt), which can assume a coiled-coil fold (Cc). We previously found that Cc structures mediate the aggregation and toxicity of polyQ Htt. Since polyQ Htt aggregates were previously found to be internalized by cells, here we hypothesize that Cc structures might be implicated in the intercellular propagation of Htt aggregates. To test this hypothesis, we performed experiments using human cell lines expressing Htt proteins with different probabilities to acquire a Cc fold. We found that Htt with reduced Cc structures were released significantly less compared to Htt with intact Cc structures. We also found that Cc structures mediate the internalization of Htt proteins in recipient cells. Together, these results underline the importance of the Cc structure in the process of intercellular propagation of Htt polyQ aggregates and suggest that interfering with Cc formation might be a therapeutic strategy for HD. Full article

Background/Objectives: The transcription factor 7-like 2 (TCF7L2) rs7903146 polymorphism has been strongly associated with type 2 diabetes mellitus (T2DM) in various populations; however, its impact on different ethnic groups is not fully understood. Given the distinct minor allele frequency in Chinese populations, this study aimed to analyze the association of rs7903146 with the risk of T2DM in a Han Chinese cohort and its relationship with relevant clinical parameters. Methods: We conducted a case–control study including 600 patients with type 2 diabetes mellitus (T2DM) and 511 sex-matched non-diabetic controls of Han Chinese descent. The TCF7L2 rs7903146 (C/T) polymorphism was genotyped using a TaqMan™ SNP assay. Clinical parameters, including body mass index (BMI), fasting plasma glucose, hemoglobin A1c, lipid profile, and high-sensitivity C-reactive protein (hs-CRP), were compared between genotypes. Logistic regression analyses were performed under a dominant genetic model (CT/TT vs. CC), adjusting for age, sex, systolic and diastolic blood pressure, BMI, and smoking status. Subgroup analyses were conducted by sex, BMI category, age at diagnosis, and family history of T2DM. Given the exploratory nature of this study and the low frequency of the TT genotype, no formal correction for multiple testing was applied. Results: Frequencies of the CT and TT genotypes were higher in the diabetic group (p = 0.045) and were significantly associated with an increased risk of T2DM under a dominant genetic model (adjusted OR = 2.24, p = 0.025). Individuals with CT/TT genotypes had elevated fasting glucose and hs-CRP levels; these genotypes were also linked to higher BMI in the female T2DM patients. The T allele frequency varied across ethnic groups, being lowest in East Asians and highest in Latin (Brazilian/mixed ancestry) populations. Mechanistically, the T allele may contribute to T2DM via altered TCF7L2 expression, impaired insulin secretion, inflammation, and metabolic dysregulation. Conclusions: The TCF7L2 rs7903146 T allele was associated with an increased risk of T2DM and higher fasting glucose and hs-CRP levels in this Han Chinese cohort. The CT/TT genotypes were also associated with higher BMI in the female T2DM patients. While the findings are consistent with the known effects of this variant in other populations, mechanistic hypotheses such as the involvement of inflammatory or metabolic pathways remain hypothetical and warrant further functional validation. Full article