Search Results (138)

In response to water scarcity and low agricultural water-use efficiency in arid regions in Northwest China, this study conducted field experiments in Wuwei, Gansu Province, from 2023 to 2024. It aimed to develop a water stress diagnosis model for spring maize to provide a scientific basis for precision irrigation and water management. In this work, two irrigation methods—plastic film-mulched drip irrigation (FD, where drip lines are laid on the soil surface and covered with film) and plastic film-mulched shallow-buried drip irrigation (MD, where drip lines are buried 3–7 cm below the surface under film)—were tested under five irrigation gradients. Multispectral UAV remote sensing data were collected from key growth stages (i.e., the jointing stage, the tasseling stage, and the grain filling stage). Then, vegetation indices were extracted, and the leaf water content (LWC) was retrieved. LWC inversion models were established using Partial Least Squares Regression (PLSR), Random Forest (RF), and Support Vector Regression (SVR). Different irrigation treatments significantly affected LWC in spring maize, with higher LWC under sufficient water supply. In the correlation analysis, plant height (hc) showed the strongest correlation with LWC under both MD and FD treatments, with R² values of −0.87 and −0.82, respectively. Among the models tested, the RF model under the MD treatment achieved the highest prediction accuracy (training set: R² = 0.98, RMSE = 0.01; test set: R² = 0.88, RMSE = 0.02), which can be attributed to its ability to capture complex nonlinear relationships and reduce multicollinearity. This study can provide theoretical support and practical pathways for precision irrigation and integrated water–fertilizer regulation in smart agriculture, boasting significant potential for broader application of such models. Full article

Maize (Zea mays) is a key crop, with its growth impacted by drought stress. Accurate, non-destructive assessment of drought severity is crucial for precision agriculture. VIS/NIR reflectance spectroscopy is widely used for estimating plant parameters and detecting stress. However, the relationship between key parameters—such as chlorophyll and water content—and VIS/NIR spectra under drought conditions in maize remains unclear, lacking comprehensive models and validation. This study aims to develop a non-destructive and accurate method for predicting chlorophyll and water content in maize leaves under drought stress using VIS/NIR spectroscopy. Specifically, maize leaf reflectance spectra were collected under varying drought stress conditions, and the effects of different spectral preprocessing methods, dimensionality reduction techniques, and machine learning algorithms were evaluated. An optimal data processing pipeline was systematically established and deployed on an edge computing unit to enable rapid, non-destructive prediction of chlorophyll and water content in maize leaves. The experimental results demonstrated that the combination of stepwise regression (SR) for feature selection and a stacking regression model achieved the best performance for chlorophyll content prediction (${\mathrm{R}}_{\mathrm{p}}^2$ = 0.8740, RMSE_p = 0.2768). For leaf water content prediction, random forest (RF) feature selection combined with a stacking model yielded the highest accuracy (${\mathrm{R}}_{\mathrm{p}}^2$ = 0.7626, RMSE_p = 4.12%). This study confirms the effectiveness and potential of integrating VIS/NIR spectroscopy with machine learning algorithms for monitoring drought stress in maize, offering a valuable theoretical foundation and practical reference for non-destructive crop physiological monitoring in precision agriculture. Full article

Canopy conductance (G_c) is an important biological constant for quantifying the water vapor flux at the canopy-atmosphere interface, reflecting the coupling strength between crop transpiration and microclimate. To elucidate how mulching modulates G_c dynamics under varying environments, we measured the transpiration of maize based on thermal equilibrium method from 2020 and 2021, synchronously recording solar radiation (R_s), temperature (T), relative humidity (RH), and vapor pressure deficit (VPD) under no-mulching (NM), transparent film (TFM), black film (BM), and straw mulching (SM) treatments in the North China Plain. The results showed that in the near-surface microenvironment, at early stages (seedling-jointing), compared to the NM treatment, TFM and BM treatments unexpectedly reduced temperature by 0.1–1.1% while increasing humidity by 0.2–4.0%, lowering VPD by 0.7–15.5%, contradicting presumed warming effects. During tasseling-filling stages, both plastic films elevated temperature by 3.5–5.2%, decreased humidity by 5.2–6.9%, and sharply increased VPD by 23.4–27.6%, inducing heat-VPD coupling stress. Throughout the entire growth period, SM treatment resulted in an initial increase followed by a decrease in temperature, but the decrease in humidity and increase in VPD occurred earlier and smoothly compared to film mulching treatment in the near-surface microenvironment. All treatments increased average temperature but decreased average humidity in the near-ground microenvironment throughout growth stages, ultimately leading to an increase in average VPD. In addition, all treatments increased G_c at noon by 10.3–81.2%. Under different solar radiation conditions, TFM, BM, and SM treatments increased the reference conductance (G_cR) but did not always increase G_c sensitivity to VPD (m). We propose a specific mulching strategy: Using black or transparent plastic film mulching in arid/cold regions and straw mulching in high-temperature and drought-prone/rain-fed agricultural areas can reconcile the trade-off between microclimate optimization and physiological adaptation, advancing precision water management in arid-prone croplands. Full article

Soil compaction is considered as one of the main factors limiting plant growth. Understanding the variation in sap flow affected by soil compaction is of vital importance for precision agriculture. In this study, a two-year field experiment with three levels of soil compaction (i.e., NC, no compaction; MC, moderate compaction; and SC, severe compaction) was conducted in the black soil region of Northeast China. Results revealed that soil compaction had a significant impact on soil properties, soil water content, and plant growth parameters, which ultimately affected the sap flow rate of maize. The average daily sap flow rates of MC and SC decreased by 15.89% and 29.12% in comparison to those of NC in 2023, and decreased by 51.53% and 57.11% in comparison to those of NC in 2024, respectively. Net radiation and vapor pressure deficit were the two most important meteorological variables affecting sap flow rate. In addition, the relationship between sap flow rate and meteorological variables was independent of the level of soil compaction stress. Daily sap flow rate exhibited a strong linear relationship with leaf area index and stem diameter, but showed no significant correlation with plant height. Additionally, daily sap flow rate was well correlated with root length density in the 0–60 cm soil layer. Furthermore, daily sap flow rate was significantly affected by soil water content of the 0–60 cm soil layer, but there was no significant correlation between daily sap flow rate and penetration resistance. Moreover, cumulative sap flow rate was negatively correlated with soil bulk density in both the top layer (0–20 cm) and sub-layer (20–40 cm). Our results provide a scientific basis for understanding the relationship between maize sap flow and soil compaction. More precise and systematic characterization of soil compaction, especially in relation to root growth, is needed to explore the underlying mechanisms of soil compaction on plant sap flow in the future. Full article

In recent years, the application of deep learning technology in the field of food engineering has developed rapidly. As an essential food raw material and processing target, the number of kernels per maize plant is a critical indicator for assessing crop growth and predicting yield. To address the challenges of frequent target ID switching, high falling speed, and the limited accuracy of traditional methods in practical production scenarios for maize kernel falling count, this study designs and implements a real-time kernel falling counting system based on a Convolutional Neural Network (CNN). The system captures dynamic video streams of kernel falling using a high-speed camera and innovatively integrates the YOLOv8 object detection framework with the ByteTrack multi-object tracking algorithm to establish an efficient and accurate kernel trajectory tracking and counting model. Experimental results demonstrate that the system achieves a tracking and counting accuracy of up to 99% under complex falling conditions, effectively overcoming counting errors caused by high-speed motion and object occlusion, and significantly enhancing robustness. This system combines high intelligence with precision, providing reliable technical support for automated quality monitoring and yield estimation in food processing production lines, and holds substantial application value and prospects for widespread adoption. Full article

Optimal application of nitrogen fertilizer is critical for soil characteristics and soil health. This study examined the effects of three rates of nitrogen fertilizer applications, which are lower rate (Treatment 1 (T1)-241 kg/ha), recommended rate (Treatment 2 (T2)-269 kg/ha), and higher rate (Treatment 3 (T3)-297 kg/ha), and their impacts on soil temperature, soil moisture and soil electrical conductivity at two different depths (0–30 cm and 30–60 cm) in maize cultivation at the Prairie View A & M university research farm in Texas. Soil moisture, soil temperature, and electrical conductivity (EC) sensors were installed in 27 plots to collect these data. Results showed that EC is lower at surface depth with all fertilizer application rates than at root zone soil depths. In the meantime, EC is increasing in the root zone soil depth with the increase in fertilizer rate. This study indicated that the moderate application (269 kg/ha, T2) which is also recommended rate, showed better soil health parameters and efficiency in comparison to other application rates maintaining stable and moderate electrical conductivity values (0.2 mS/cm at depth 2) and the highest median moisture content at the significant root zone depth (about 0.135 m³/m³), reducing nutrient leaching and salt accumulation. Also, a humid, warm climate in southern Texas specifically affects increasing nitrogen losses via leaching, denitrification, and volatilization compared to cooler regions, which requires higher application rates. Plant growth and yield results further confirmed that the recommended rate achieved the greatest plant height (157.48 cm) compared to T1 (153.07 cm). Ear diameters were also higher at the recommended rate, reaching 4.65 cm ears than in Treatment 3. However, grain productivity was highest under the lower fertilizer rate T1, with wet and dry yields of 11,567 kg/ha and 5959 kg/ha, respectively, compared to 10,033 kg/ha (wet) and 5047 kg/ha (dry) at T2, and 7446 kg/ha (wet) and 4304 kg/ha (dry) at T3. These findings suggest that while the moderate fertilizer rate (269 kg/ha) enhances soil health and crop growth consistency, the lower rate (241 kg/ha) can maximize productivity under the humid, warm conditions of southern Texas. This research highlights the need for precise nitrogen management strategies that balance soil health with crop yield. Full article

Maize (Zea mays L.) is a key global cereal crop with significant relevance to food security. Maize yield prediction is challenged by cultivar diversity and varying management practices. This preliminary study was conducted at Youyi Farm, Heilongjiang Province, China. Three maize cultivars (Songyu 438, Dika 1220, Dika 2188), two fertilization rates (700 and 800 kg·ha⁻¹), and three planting densities (70,000, 75,000, and 80,000 plants·ha⁻¹) were evaluated across 18 distinct cropping treatments. During the V6 (Vegetative 6-leaf stage), VT (Tasseling stage), R3 (Milk stage), and R6 (Physiological maturity) growth stages of maize, multi-temporal canopy spectral images were acquired using an unmanned aerial vehicle (UAV) equipped with a multispectral sensor. In situ measurements of key agronomic traits, including plant height (PH), stem diameter (SD), leaf area index (LAI), and relative chlorophyll content (SPAD), were conducted. The optimal vegetation indices (VIs) and agronomic traits were selected for developing a maize yield prediction model using the random forest (RF) algorithm. Results showed the following: (1) Vegetation indices derived from the red-edge band, particularly the normalized difference red-edge index (NDRE), exhibited a strong correlation with maize yield (R = 0.664), especially during the tasseling to milk ripening stage; (2) The integration of LAI and SPAD with NDRE improved model performance, achieving an R² of 0.69—an increase of 23.2% compared to models based solely on VIs; (3) Incorporating SPAD values from middle-canopy leaves during the milk ripening stage further enhanced prediction accuracy (R² = 0.74, RMSE = 0.88 t·ha⁻¹), highlighting the value of vertical-scale physiological parameters in yield modeling. This study not only furnishes critical technical support for the application of UAV-based remote sensing in precision agriculture at the field-plot scale, but also charts a clear direction for the synergistic optimization of multi-dimensional agronomic traits and spectral features. Full article

Rice, wheat, and maize are important food grains consumed by most of the population in Asian countries (like India, Japan, Singapore, Malaysia, China, and Thailand). These crops’ production is affected by biotic and abiotic factors that cause diseases in several parts of the crops (including leaves, stems, roots, nodes, and panicles). A severe infection affects the growth of the plant, thereby undermining the economy of a country, if not detected at an early stage. This may cause extensive damage to crops, resulting in decreased yield and productivity. Early safeguarding methods are overlooked because of farmers’ lack of awareness and the variety of crop diseases. This causes significant crop damage and can consequently lower productivity. In this manuscript, a lightweight vision transformer (MaxViT) with 814.7 K learnable parameters and 85 layers is designed for classifying crop diseases in paddy and wheat. The MaxViT DNN architecture consists of a convolutional block attention module (CBAM), squeeze and excitation (SE), and depth-wise (DW) convolution, followed by a ConvNeXt module. This network architecture enhances feature representation by eliminating redundant information (using CBAM) and aggregating spatial information (using SE), and spatial filtering by the DW layer cumulatively enhances the overall classification performance. The proposed model was tested using a paddy dataset (with 7857 images and eight classes, obtained from local paddy farms in Lalgudi district, Tiruchirappalli) and a wheat dataset (with 5000 images and five classes, downloaded from the Kaggle platform). The model’s classification performance for various diseases has been evaluated based on accuracy, sensitivity, specificity, mean accuracy, precision, F1-score, and MCC. During training and testing, the model’s overall accuracy on the paddy dataset was 99.43% and 98.47%, respectively. Training and testing accuracies were 94% and 92.8%, respectively, for the wheat dataset. Ablation analysis was carried out to study the significant contribution of each module to improving the performance. It was found that the model’s performance was immune to the presence of noise. Additionally, there are a minimal number of parameters involved in the proposed model as compared to pre-trained networks, which ensures that the model trains faster. Full article

Accurate maize yield forecasting under climate uncertainty remains a critical challenge for global food security, yet existing studies predominantly rely on single-model frameworks, limiting generalizability and actionable insights. This study selected three regions, specifically Dali, Lijiang, and Zhaotong, and collected data on 12 agronomic traits of 114 varieties, along with eight sets of meteorological data, covering the period from 2019 to 2023. We employed three machine learning models: Random Forest (RF), Support Vector Machine (SVM), and XGBoost. The results revealed a strong correlation between yield and multiple agronomic traits, particularly grain weight per spike (GWPS) and hundred-kernel weight (HKW). Notably, the XGBoost model emerged as the top performer across all three regions. The model achieved the lowest RMSE (0.22–191.13) and a good R² (0.98–0.99), demonstrating exceptional predictive accuracy for yield-related traits. The comparative analysis revealed that XGBoost exhibited superior accuracy and stability compared to RF and SVM. Through feature importance analysis, four critical determinants of yield were identified: GWPS, shelling percentage (SP), growth period (GP), and plant height (PH). Furthermore, partial dependence plots (PDPs) provided deeper insights into the nonlinear interactive effects between GWPS, SP, GP, PH, and yield, offering a more comprehensive understanding of their complex relationships. This study presents an innovative, data-driven methodology designed to accurately forecast corn yield across diverse locations. This approach offers valuable scientific insights that can significantly enhance precision agricultural practices by enabling the precise tailoring of fertilizer usage and irrigation strategies. The results highlight the importance of integrating agronomic and meteorological data in yield forecasting, paving the way for development of agricultural decision-support systems in the context of future climate change scenarios. This study presents an innovative, data-driven methodology designed to accurately forecast corn yield across diverse locations. This approach offers valuable scientific insights that can significantly enhance precision agricultural practices by enabling the precise tailoring of fertilizer usage and irrigation strategies. Full article

Accurate detection of maize tassels plays a crucial role in yield estimation of maize in precision agriculture. Recently, UAV and deep learning technologies have been widely introduced in various applications of field monitoring. However, complex field backgrounds pose multiple challenges against the precision detection of maize tassels, including maize tassel multi-scale variations caused by varietal differences and growth stage variations, intra-class occlusion, and background interference. To achieve accurate maize tassel detection in UAV images under complex field backgrounds, this study proposes an MSMT-RTDETR detection model. The Faster-RPE Block is first designed to enhance multi-scale feature extraction while reducing model Params and FLOPs. To improve detection performance for multi-scale targets in complex field backgrounds, a Dynamic Cross-Scale Feature Fusion Module (Dy-CCFM) is constructed by upgrading the CCFM through dynamic sampling strategies and multi-branch architecture. Furthermore, the MPCC3 module is built via re-parameterization methods, and further strengthens cross-channel information extraction capability and model stability to deal with intra-class occlusion. Experimental results on the MTDC-UAV dataset demonstrate that the MSMT-RTDETR significantly outperforms the baseline in detecting maize tassels under complex field backgrounds, where a precision of 84.2% was achieved. Compared with Deformable DETR and YOLOv10m, improvements of 2.8% and 2.0% were achieved, respectively, in the mAP₅₀ for UAV images. This study proposes an innovative solution for accurate maize tassel detection, establishing a reliable technical foundation for maize yield estimation. Full article

Soil available nitrogen (AN) is a critical nutrient for plant absorption and utilization. Accurately mapping its spatial distribution is essential for improving crop yields and advancing precision agriculture. In this study, 188 AN soil samples (0–20 cm) were collected at Heshan Farm, Nenjiang County, Heihe City, Heilongjiang Province, in 2023. The soil available nitrogen content ranged from 65.81 to 387.10 mg kg⁻¹, with a mean value of 213.85 ± 61.16 mg kg⁻¹. Sentinel-2 images and normalized vegetation index (NDVI) and enhanced vegetation index (EVI) time series data were acquired on the Google Earth Engine (GEE) platform in the study area during the bare soil period (April, May, and October) and the growth period (June–September). These remote sensing variables were combined with soil sample data, crop type information, and crop growth period data as predictive factors and input into a Random Forest (RF) model optimized using the Optuna hyperparameter tuning algorithm. The accuracy of different strategies was evaluated using 5-fold cross-validation. The research results indicate that (1) the introduction of growth information at different growth periods of soybean and maize has different effects on the accuracy of soil AN mapping. In soybean plantations, the introduction of EVI data during the pod setting period increased the mapping accuracy R² by 0.024–0.088 compared to other growth periods. In maize plantations, the introduction of EVI data during the grouting period increased R² by 0.004–0.033 compared to other growth periods, which is closely related to the nitrogen absorption intensity and spectral response characteristics during the reproductive growth period of crops. (2) Combining the crop types and their optimal period growth information could improve the mapping accuracy, compared with only using the bare soil period image (R² = 0.597)—the R² increased by 0.035, the root mean square error (RMSE) decreased by 0.504%, and the mapping accuracy of R² could be up to 0.632. (3) The mapping accuracy of the bare soil period image differed significantly among different months, with a higher mapping accuracy for the spring data than the fall, the R² value improved by 0.106 and 0.100 compared with that of the fall, and the month of April was the optimal window period of the bare soil period in the present study area. The study shows that when mapping the soil AN content in arable land, different crop types, data collection time, and crop growth differences should be considered comprehensively, and the combination of specific crop types and their optimal period growth information has a greater potential to improve the accuracy of mapping soil AN content. This method not only opens up a new technological path to improve the accuracy of remote sensing mapping of soil attributes but also lays a solid foundation for the research and development of precision agriculture and sustainability. Full article

The increasing concentration of greenhouse gases is amplifying the global risk of drought on crop productivity. This study sought to investigate the effects of drought on the growth of maize (Zea mays L.) seedlings. A total of 78 maize hybrids were employed in this study to replicate drought conditions through the potting method. The maize seedlings were subjected to a 10-day period of water breakage following a standard watering cycle until they reached the third leaf collar (V3) stage. Parameters including plant height, stem diameter, chlorophyll content, and root number were assessed. The eight phenotypic traits include the fresh and dry weights of both the aboveground and underground parts. Three machine learning methods—random forest (RF), K-nearest neighbor (KNN), and extreme gradient boosting (XGBoost)—were employed to systematically analyze the relevant traits of maize seedlings’ drought tolerance and to assess their predictive performance in this regard. The findings indicated that plant height, aboveground weight, and chlorophyll content constituted the primary indices for phenotyping maize seedlings under drought conditions. The XGBoost model demonstrated optimal performance in the classification (AUC = 0.993) and regression (R² = 0.863) tasks, establishing itself as the most effective prediction model. This study provides a foundation for the feasibility and reliability of screening drought-tolerant maize varieties and refining precision breeding strategies. Full article

Heat shock transcription factor (Hsf) plays a pivotal role in regulating plant growth, development, and stress responses. Hsf activates or represses target gene transcription by binding to the heat shock element (HSE) of downstream genes. However, the specific interaction sites between Hsf and the HSE in the promoter remain unclear. In this study, the critical amino acid residues of ZmHsf17 and the paralogous ZmHsf05 involved in DNA binding were identified using molecular docking models, site-directed mutagenesis, and the electrophoretic mobility shift assay (EMSA). The results reveal that both ZmHsf17 and ZmHsf05 bind to the HSE of the ZmPAH1 promoter via a conserved arginine residue located in the α3 helix of their DNA-binding domains. Sequence substitution experiments among distinct HSEs demonstrated that flanking sequences upstream and downstream of the HSE core synergistically contribute to the specificity of DNA-binding domain recognition. Comparative evolutionary analysis of DNA-binding domain sequences from 25 phylogenetically diverse species reveals that the α3 helix constitutes the most conserved structural element. This study elucidates the key interaction sites between maize HsfA2 and its target genes, providing theoretical insights into the binding specificity to the HSEs of the plant’s Hsf family and the functional divergence. Additionally, these findings offer new targets for the precise engineering of Hsf proteins and synthetic HSEs. Full article

Precise estimation of the leaf area index (LAI) is vital in efficient maize growth monitoring and precision farming. Traditional LAI measurement methods are often destructive and labor-intensive, while techniques relying solely on spectral data suffer from limitations such as spectral saturation. To overcome these difficulties, the study integrated computer vision techniques with UAV-based remote sensing data to establish a rapid and non-invasive method for estimating the LAI in maize. Multispectral imagery of maize was acquired via UAV platforms across various phenological stages, and vegetation features were derived based on the Excess Green (ExG) Index and the Hue–Saturation–Value (HSV) color space. LAI standardization was performed through edge detection and the cumulative distribution function. The proposed LAI estimation model, named VisLAI, based solely on visible light imagery, demonstrated high accuracy, with R² values of 0.84, 0.75, and 0.50, and RMSE values of 0.24, 0.35, and 0.44 across the big trumpet, tasseling–silking, and grain filling stages, respectively. When HSV-based optimization was applied, VisLAI achieved even better performance, with R² values of 0.92, 0.90, and 0.85, and RMSE values of 0.19, 0.23, and 0.22 at the respective stages. The estimation results were validated against ground-truth data collected using the LAI-2200C plant canopy analyzer and compared with six machine learning algorithms, including Gradient Boosting (GB), Random Forest (RF), Ridge Regression (RR), Support Vector Regression (SVR), and Linear Regression (LR). Among these, GB achieved the best performance, with R² values of 0.88, 0.88, and 0.65, and RMSE values of 0.22, 0.25, and 0.34. However, VisLAI consistently outperformed all machine learning models, especially during the grain filling stage, demonstrating superior robustness and accuracy. The VisLAI model proposed in this study effectively utilizes UAV-captured visible light imagery and computer vision techniques to achieve accurate, efficient, and non-destructive estimation of maize LAI. It outperforms traditional and machine learning-based approaches and provides a reliable solution for real-world maize growth monitoring and agricultural decision-making. Full article

Maize tassels are critical phenotypic organs in maize, and their quantity is essential for determining tasseling stages, estimating yield potential, monitoring growth status, and supporting crop breeding programs. However, tassel identification in complex field environments presents significant challenges due to occlusion, variable lighting conditions, multi-scale target complexities, and the asynchronous and irregular growth patterns characteristic of maize tassels. In response to these challenges, this paper presents a DMSF-YOLO model for maize tassel detection. In the network’s backbone front, conventional convolutions are replaced with conditional parameter convolutions (CondConv) to enhance feature extraction capabilities. A novel DMSF-P2 network architecture is designed, including a multi-scale fusion module (SSFF-D), a scale-splicing module (TFE), and a small object detection layer (P2), which further enhances the model’s feature fusion capabilities. By integrating a dynamic detection head (Dyhead), superior recognition accuracy for maize tassels across various scales is achieved. Additionally, the Wise-IoU loss function is used to improve localization precision and strengthen the model’s adaptability. Experimental results demonstrate that on our self-built maize tassel detection dataset, the proposed DMSF-YOLO model shows remarkable superiority compared with the baseline YOLOv8n model, with precision (P), recall (R), $mA{P}_{50}$, and $mA{P}_{50:95}$ increasing by 0.5%, 3.4%, 2.4%, and 3.9%, respectively. This approach enables accurate and reliable maize tassel detection in complex field environments, providing effective technical support for precision field management of maize crops. Full article