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14 pages, 513 KiB  
Article
Set-Valued Approximation—Revisited and Improved
by David Levin
Mathematics 2025, 13(7), 1194; https://doi.org/10.3390/math13071194 - 4 Apr 2025
Viewed by 39
Abstract
We address the problem of approximating a set-valued function F, where F:[a,b]K(Rd) given its samples {F(a+ih)}Ni=0, with [...] Read more.
We address the problem of approximating a set-valued function F, where F:[a,b]K(Rd) given its samples {F(a+ih)}Ni=0, with h=(ba)/N. We revisit an existing method that approximates set-valued functions by interpolating signed-distance functions. This method provides a high-order approximation for general topologies but loses accuracy near points where F undergoes topological changes. To address this, we introduce new techniques that enhance efficiency and maintain high-order accuracy across [a,b]. Building on the foundation of previous publication, we introduce new techniques to improve the method’s efficiency and extend its high-order approximation accuracy throughout the entire interval [a,b]. Particular focus is placed on identifying and analyzing the behavior of F near topological transition points. To address this, two algorithms are introduced. The first algorithm employs signed-distance quasi-interpolation, incorporating specialized adjustments to effectively handle singularities at points of topological change. The second algorithm leverages an implicit function representation of Graph(F), offering an alternative and robust approach to its approximation. These enhancements improve accuracy and stability in handling set-valued functions with changing topologies. Full article
(This article belongs to the Special Issue Advances in Approximation Theory and Numerical Functional Analysis)
14 pages, 588 KiB  
Article
Measurement Uncertainty in the Totalisation of Quantity and Energy Measurement in Gas Grids
by Adriaan M. H. van der Veen, Kjetil Folgerø and Federica Gugole
Gases 2025, 5(2), 7; https://doi.org/10.3390/gases5020007 - 3 Apr 2025
Viewed by 76
Abstract
The total quantity and energy delivered through a gas grid is calculated using simple formulæ that sum the increments measured at regular time intervals. These calculations are described in international standards (e.g., ISO 15112 and EN 1776) and guidelines (e.g., OIML R140). These [...] Read more.
The total quantity and energy delivered through a gas grid is calculated using simple formulæ that sum the increments measured at regular time intervals. These calculations are described in international standards (e.g., ISO 15112 and EN 1776) and guidelines (e.g., OIML R140). These guidelines recommend that the associated measurement uncertainty is evaluated assuming the measurement results to be mutually independent. This assumption leads to the underestimation of the measurement uncertainty. To address the growing concern among transmission and distribution system operators, the underlying assumptions of these uncertainty evaluations are revisited and reworked to be more adequate. The dependence of measurement results coming from, e.g., the same flow meter and gas chromatograph will be assessed for correlations, as well as other effects, such as the effect of the chosen mathematical approximation of the totalisation integral and fluctuations in the flow rate and gas quality. In this paper, an outline is given for improvements that can be implemented in the measurement models to render them more responsive to the error structure of the measurement data, temporal effects in these data, and the fluctuations in the gas quality and gas quantity. By impact assessment using a simple scenario involving the injection of (renewable) hydrogen into a natural gas grid, it is shown that these improvements lead to a substantive difference. This preliminary work demonstrates that correlations occur both in the instrumental measurement uncertainty and due to temporal effects in the gas grid. To obtain a fit-for-purpose uncertainty budget for custody transfer and grid balancing, it is key to enhance the current models and standards accordingly. Full article
(This article belongs to the Section Natural Gas)
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19 pages, 1236 KiB  
Article
How Customer Avoidance Leads to Customers Returning: A Longitudinal Study Concerning Online Travel Agencies
by Zerui Su and Hong-Youl Ha
J. Theor. Appl. Electron. Commer. Res. 2025, 20(1), 35; https://doi.org/10.3390/jtaer20010035 - 25 Feb 2025
Viewed by 416
Abstract
Customers’ intentions to avoid a product or service tend to be dynamic. Thus, this study aims to explore the influence of trajectory changes in customers’ avoidance after service recovery on relationship strength, negative word-of-mouth (WOM) intentions, and revisit intentions. Using a longitudinal approach [...] Read more.
Customers’ intentions to avoid a product or service tend to be dynamic. Thus, this study aims to explore the influence of trajectory changes in customers’ avoidance after service recovery on relationship strength, negative word-of-mouth (WOM) intentions, and revisit intentions. Using a longitudinal approach with three-month lag intervals, we implement a latent growth model analysis to test our proposed hypotheses. Our findings demonstrate that customers’ desire to engage in avoidance after a service failure evolves, but its impact wanes. As avoidance decreases, negative WOM intentions likewise decline, and intentions to revisit a firm (which, in this study, is a travel agency) increase, thereby attenuating an avoidance-becomes-defection effect over time. Meanwhile, relationship strength initially grows but then weakens after service recovery. In contrast, negative WOM intentions slightly decrease from the early to mid-stage, followed by an increase in the late stage. Furthermore, relationship strength does not affect negative WOM or revisit intentions at the subsequent service recovery phase. Our findings offer innovative insights into upgrading customer avoidance perspectives regarding service recovery. We also present managerial implications regarding service recovery and customer relationship strategies that vary over time. Full article
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12 pages, 535 KiB  
Article
Text Indexing for Faster Gapped Pattern Matching
by Md Helal Hossen, Daniel Gibney and Sharma V. Thankachan
Algorithms 2024, 17(12), 537; https://doi.org/10.3390/a17120537 - 23 Nov 2024
Viewed by 533
Abstract
We revisit the following version of the Gapped String Indexing problem, where the goal is to preprocess a text T[1..n] to enable efficient reporting of all occ occurrences of a gapped pattern [...] Read more.
We revisit the following version of the Gapped String Indexing problem, where the goal is to preprocess a text T[1..n] to enable efficient reporting of all occ occurrences of a gapped pattern P=P1[α..β]P2 in T. An occurrence of P in T is defined as a pair (i,j) where substrings T[i..i+|P1|) and T[j..j+|P2|) match P1 and P2, respectively, with a gap j(i+|P1|) lying within the interval [α..β]. This problem has significant applications in computational biology and text mining. A hardness result on this problem suggests that any index with polylogarithmic query time must occupy near quadratic space. In a recent study [STACS 2024], Bille et al. presented a sub-quadratic space index using space ˜O(n2δ/3), where 0δ1 is a parameter fixed at the time of index construction. Its query time is ˜O(|P1|+|P2|+nδ·(1+occ)), which is sub-linear per occurrence when δ<1. We show how to achieve a gap-sensitive query time of ˜O(|P1|+|P2|+nδ·(1+occ1δ)+g[α..β]occg·gδ) using the same space, where occg denotes the number of occurrences with gap g. This is faster when there are many occurrences with small gaps. Full article
(This article belongs to the Special Issue Selected Algorithmic Papers from IWOCA 2024)
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20 pages, 6644 KiB  
Article
Refined Coseismic Slip and Afterslip Distributions of the 2021 Mw 6.1 Yangbi Earthquake Based on GNSS and InSAR Observations
by Zheng Liu, Keliang Zhang, Weijun Gan and Shiming Liang
Remote Sens. 2024, 16(21), 3996; https://doi.org/10.3390/rs16213996 - 28 Oct 2024
Viewed by 1105
Abstract
On 21 May 2021, an Mw 6.1 earthquake occurred in Yangbi County, Dali Bai Autonomous Prefecture, Yunnan Province, with the epicenter located in an unmapped blind fault approximately 7 km west of the Weixi-Qiaohou fault (WQF) on the southeastern margin of the Qinghai–Tibetan [...] Read more.
On 21 May 2021, an Mw 6.1 earthquake occurred in Yangbi County, Dali Bai Autonomous Prefecture, Yunnan Province, with the epicenter located in an unmapped blind fault approximately 7 km west of the Weixi-Qiaohou fault (WQF) on the southeastern margin of the Qinghai–Tibetan Plateau. While numerous studies have been conducted to map the coseismic slip distribution by using the Global Navigation Satellite System (GNSS), Interferometric Synthetic Aperture Radar (InSAR) and seismic data as well as their combinations, the understanding of deformation characteristics during the postseismic stage remains limited, mostly due to the long revisiting time interval and large uncertainty of most SAR satellites. In this study, we refined coseismic slip and afterslip distributions with nonlinear inversions for both fault geometry and relaxation time. First, we determined the fault geometry and coseismic slip distribution of this earthquake by joint inversion for coseismic offsets in the line-of-sight (LOS) direction of both Sentinel-1A/B ascending and descending track images and GNSS data. Then, the descending track time series of Sentinel-1 were further fitted using nonlinear least squares to extract the coseismic and postseismic deformations. Finally, we obtained the refined coseismic slip and afterslip distributions and investigated the spatiotemporal evolution of fault slip by comparing the afterslip with aftershocks. The refined coseismic moment magnitude, which was of Mw 6.05, was smaller than Mw 6.1 or larger, which was inferred from our joint inversion and previous studies, indicating a significant reduction in early postseismic deformation. In contrast, the afterslip following the mainshock lasted for about six months and was equivalent to a moment release of an Mw 5.8 earthquake. These findings not only offer a novel approach to extracting postseismic deformation from noisy InSAR time series but also provide valuable insights into fault slip mechanisms associated with the Yangbi earthquake, enhancing our understanding of seismic processes. Full article
(This article belongs to the Special Issue Monitoring Geohazard from Synthetic Aperture Radar Interferometry)
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27 pages, 18769 KiB  
Article
A Fresh Revisit of the Issues and Improvements in Impulse Invariance Filter Design for Infinite Impulse Response Filters
by Chiman Kwan and Hal Ferguson
Electronics 2024, 13(18), 3753; https://doi.org/10.3390/electronics13183753 - 21 Sep 2024
Viewed by 783
Abstract
The objective of this paper is to first present some issues with impulse invariance filter (IIF) design during the design of digital infinite impulse response (IIR) filters. Engineers are often confused about some inconsistent observations. For instance, if the impulse response of a [...] Read more.
The objective of this paper is to first present some issues with impulse invariance filter (IIF) design during the design of digital infinite impulse response (IIR) filters. Engineers are often confused about some inconsistent observations. For instance, if the impulse response of a digital filter is designed using the impulse invariance procedure, then the analog and digital filters’ frequency and step responses are very different. Two simple remedies are presented in this paper. One is a post-processing approach that scales the frequency and step responses of the digital filter by the sampling interval T. Another one is a pre-processing approach that scales the impulse response of the analog filter by T. However, even after these remedies, there is still a steady state bias in the step response of the digital filter for certain cases where there is discontinuity in the analog impulse response. A recommendation is to include a correction term in the digital filter. After that, the steady state bias in the digital filter is then suppressed. Moreover, the MATLAB R2021a command “impinvar” needs to also include a correction term so that the frequency and step responses can be more accurate in the digital filter. Two comparative studies were carried out to compare the improved IIF filter with three competing digital IIR filter design methods. Although the above issues and improvements have been proposed by researchers in the past, many researchers, engineers, and students are still not aware of them. This paper provides a fresh revisit of these issues and improvements by using figures, equations, and examples. Proper credits are also given to those researchers who first pointed out those issues and improvements. It is hoped that through an open access journal, future rediscovery of issues and improvements in IIF can be prevented. Full article
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20 pages, 7687 KiB  
Article
Enhancing Surface Water Monitoring through Multi-Satellite Data-Fusion of Landsat-8/9, Sentinel-2, and Sentinel-1 SAR
by Alexis Declaro and Shinjiro Kanae
Remote Sens. 2024, 16(17), 3329; https://doi.org/10.3390/rs16173329 - 8 Sep 2024
Cited by 4 | Viewed by 2992
Abstract
Long revisit intervals and cloud susceptibility have restricted the applicability of earth observation satellites in surface water studies. Integrating multiple satellites offers potential for more frequent observations, yet combining different satellite sources, particularly optical and SAR satellites, presents complexities. This research explores the [...] Read more.
Long revisit intervals and cloud susceptibility have restricted the applicability of earth observation satellites in surface water studies. Integrating multiple satellites offers potential for more frequent observations, yet combining different satellite sources, particularly optical and SAR satellites, presents complexities. This research explores the data-fusion potential and limitations of Landsat-8/9 Operational Land Imager (OLI), Sentinel-2 Multispectral Instrument (MSI), and Sentinel-1 Synthetic Aperture (SAR) satellites to enhance surface water monitoring. By focusing on segmented surface water images, we demonstrate that combining optical and SAR data is generally effective and straightforward using a simple statistical thresholding algorithm. Kappa coefficients(κ) ranging from 0.80 to 0.95 indicate very strong harmony for integration across reservoirs, lakes, and river environments. In vegetative environments, integration with S1SAR shows weak harmony, with κ values ranging from 0.27 to 0.45, indicating the need for further studies. Global revisit interval maps reveal significant improvement in median revisit intervals from 15.87 to 22.81 days using L8/9 alone, to 4.51 to 7.77 days after incorporating S2, and further to 3.48 to 4.62 days after adding S1SAR. Even during wet season months, multi-satellite fusion maintained the median revisit intervals to less than a week. Maximizing all available open-source earth observation satellites is integral for advancing studies requiring more frequent surface water observations, such as flood, inundation, and hydrological modeling. Full article
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12 pages, 323 KiB  
Article
On One Approach to Obtaining Estimates of the Rate of Convergence to the Limiting Regime of Markov Chains
by Yacov Satin, Rostislav Razumchik, Alexander Zeifman and Ilya Usov
Mathematics 2024, 12(17), 2763; https://doi.org/10.3390/math12172763 - 6 Sep 2024
Viewed by 877
Abstract
We revisit the problem of the computation of the limiting characteristics of (in)homogeneous continuous-time Markov chains with the finite state space. In general, it can be performed only numerically. The common rule of thumb is to interrupt calculations after quite some time, hoping [...] Read more.
We revisit the problem of the computation of the limiting characteristics of (in)homogeneous continuous-time Markov chains with the finite state space. In general, it can be performed only numerically. The common rule of thumb is to interrupt calculations after quite some time, hoping that the values at some distant time interval will represent the sought-after solution. Convergence or ergodicity bounds, when available, can be used to answer such questions more accurately; i.e., they can indicate how to choose the position and the length of that distant time interval. The logarithmic norm method is a general technique that may allow one to obtain such bounds. Although it can handle continuous-time Markov chains with both finite and countable state spaces, its downside is the need to guess the proper similarity transformations, which may not exist. In this paper, we introduce a new technique, which broadens the scope of the logarithmic norm method. This is achieved by firstly splitting the generator of a Markov chain and then merging the convergence bounds of each block into a single bound. The proof of concept is illustrated by simple examples of the queueing theory. Full article
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18 pages, 15605 KiB  
Article
Assessing Slip Rates on the Xianshuihe Fault Using InSAR with Emphasis on Phase Unwrapping Error and Atmospheric Delay Corrections
by Peiyan Xi, Xing Li, Chuang Song, Bin Wang, Zhi Yin and Shuai Wang
Remote Sens. 2024, 16(11), 1872; https://doi.org/10.3390/rs16111872 - 24 May 2024
Viewed by 1124
Abstract
Located on the southeastern periphery of the Tibetan Plateau, the Xianshuihe fault (XSHF) is an active left-lateral strike-slip fault renowned for its frequent and intensive seismic activities. This highlights the necessity of employing advanced geodetic methodologies to precisely evaluate the fault kinematics and [...] Read more.
Located on the southeastern periphery of the Tibetan Plateau, the Xianshuihe fault (XSHF) is an active left-lateral strike-slip fault renowned for its frequent and intensive seismic activities. This highlights the necessity of employing advanced geodetic methodologies to precisely evaluate the fault kinematics and seismic hazard potential along this fault. Among these techniques, interferometric synthetic aperture radar (InSAR) stands out for its high spatial resolution and regular revisit intervals, enabling accurate mapping of interseismic deformation associated with fault motion. However, the precision of InSAR in measuring deformation encounters several challenges, particularly artifacts stemming from phase unwrapping errors and atmospheric phase delays. In this study, we utilize ascending and descending Sentinel-1 InSAR images spanning from January 2017 to January 2023 to drive the line-of-sight (LOS) mean crustal velocities associated with the XSHF with emphasis on phase unwrapping errors and atmospheric delay corrections. Then, the reliability of the derived LOS velocities is assessed using independent observations from the Global Navigation Satellite System (GNSS). The inferred fault slip rate along the XSHF shows significant along-strike variations, gradually decreasing from ~11.1 mm/yr at the Luhuo section to ~6.6 mm/yr at the Kangding section and then sharply increasing to ~13.0 mm/yr towards its eastern terminus at the Moxi section. The fault locking depth shows similar along-strike variations, decreasing from ~19.5 km in the northwestern part to ~4.8 km at the Kangding section, before increasing to 19.6 km at the Moxi segment. Notably, apparent surface fault creeping, characterized by a slip rate of ~2.7 mm/yr, is observed at the Kangding segment, likely resulting from postseismic slip following the 2014 Mw 6.3 Kangding earthquake. Full article
(This article belongs to the Section Remote Sensing in Geology, Geomorphology and Hydrology)
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14 pages, 4136 KiB  
Article
Development of Deployable Reflector Antenna for the SAR-Satellite: Part 1. Design and Analysis of the Main Reflector Using Honeycomb Sandwich Composite Structure
by Hyun-Guk Kim, Dong-Geon Kim, Ryoon-Ho Do, Kyung-Rae Koo and Young-Joon Yu
Appl. Sci. 2024, 14(4), 1590; https://doi.org/10.3390/app14041590 - 16 Feb 2024
Cited by 5 | Viewed by 3210
Abstract
The deployable reflector antenna based on the synthetic aperture radar is a satellite component that consists of a unit structure in the form of a folded reflector. During the launch process, this satellite antenna is in the stowed condition to improve storage efficiency. [...] Read more.
The deployable reflector antenna based on the synthetic aperture radar is a satellite component that consists of a unit structure in the form of a folded reflector. During the launch process, this satellite antenna is in the stowed condition to improve storage efficiency. It is then deployed to perform the space mission in the on-orbit condition. Due to these structural characteristics of the deployable reflector antenna, the reflector is possible to be loaded in the limited volume of the launch vehicle with the reduced size. Additionally, because the deployable reflector antenna is made by the lightweight material of the carbon fiber reinforced polymer and honeycomb core, it can reduce the launching cost and improve the revisit interval. In this paper, the conceptual design of the main reflector of the deployable reflector antenna was conducted. The main reflector was designed as the honeycomb sandwich composite structure. To design the main reflector, the stacking sequence of the composite material and honeycomb core was investigated to maximize the structural stiffness and minimize the antenna’s mass. Subsequently, finite element analyses including modal, quasi-static, structural–thermal coupling, and transient response were performed to numerically evaluate the structural performance of the lightweight composite reflector antenna. Full article
(This article belongs to the Section Aerospace Science and Engineering)
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18 pages, 6002 KiB  
Article
Study on Electron Density Anomalies Possibly Related to Earthquakes Based on CSES Observations
by Chengcheng Han, Rui Yan, Dedalo Marchetti, Weixing Pu, Zeren Zhima, Dapeng Liu, Song Xu, Hengxin Lu and Na Zhou
Remote Sens. 2023, 15(13), 3354; https://doi.org/10.3390/rs15133354 - 30 Jun 2023
Cited by 3 | Viewed by 1894
Abstract
This research examines the correlation between seismic activity and variations in ionospheric electron density (Ne) using the data from the Langmuir probe (LAP) onboard the China Seismo-Electromagnetic Satellite (CSES) during nighttime. Statistical analysis of Ms ≥ 6.8 earthquakes that occurred globally between August [...] Read more.
This research examines the correlation between seismic activity and variations in ionospheric electron density (Ne) using the data from the Langmuir probe (LAP) onboard the China Seismo-Electromagnetic Satellite (CSES) during nighttime. Statistical analysis of Ms ≥ 6.8 earthquakes that occurred globally between August 2018 and March 2023 is conducted, as well as Ms ≥ 6.0 earthquakes in China during the same period, using the quartile analysis method for fixed revisiting orbits. The main conclusions are that: (1) the larger the magnitude of the earthquake, the more anomalous the phenomena that appear; (2) the anomalies on the east side of the epicenter are significantly higher than those on the west side, and the anomalies in the Northern Hemisphere are mostly distributed southward from the epicenter, while those in the Southern Hemisphere are mostly distributed northward from the epicenter; (3) anomalies appear with a higher frequency on several specific time intervals, including the day of the earthquake (likely co-seismic effect) and 2, 7, and 11 days before the earthquake (possible precursor candidates); and (4) for the 15 earthquakes of Ms ≥ 6.0 in China over the past five years, anomalous Ne mainly occurred southwest of the epicenter, with the highest frequency observed 5 days before the earthquake, and there were continuous anomalous phenomena between 9 days and 5 days before the earthquake. This study concludes that Ne, measured by CSES, can play a fundamental role in studying earthquake-related ionospheric disturbances. Full article
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10 pages, 292 KiB  
Article
Some Properties of Interval Shapley Values: An Axiomatic Analysis
by Shinichi Ishihara and Junnosuke Shino
Games 2023, 14(3), 50; https://doi.org/10.3390/g14030050 - 15 Jun 2023
Cited by 3 | Viewed by 1834
Abstract
Interval games are an extension of cooperative coalitional games, in which players are assumed to face payoff uncertainty. Characteristic functions thus assign a closed interval instead of a real number. This study revisits two interval game versions of Shapley values (i.e., the interval [...] Read more.
Interval games are an extension of cooperative coalitional games, in which players are assumed to face payoff uncertainty. Characteristic functions thus assign a closed interval instead of a real number. This study revisits two interval game versions of Shapley values (i.e., the interval Shapley value and the interval Shapley-like value) and characterizes them using an axiomatic approach. For the interval Shapley value, we show that the existing axiomatization can be generalized to a wider subclass of interval games called size monotonic games. For the interval Shapley-like value, we show that a standard axiomatization using Young’s strong monotonicity holds on the whole class of interval games. Full article
(This article belongs to the Section Cooperative Game Theory and Bargaining)
19 pages, 7975 KiB  
Article
Trusted Autonomous Operations of Distributed Satellite Systems Using Optical Sensors
by Kathiravan Thangavel, Dario Spiller, Roberto Sabatini, Stefania Amici, Nicolas Longepe, Pablo Servidia, Pier Marzocca, Haytham Fayek and Luigi Ansalone
Sensors 2023, 23(6), 3344; https://doi.org/10.3390/s23063344 - 22 Mar 2023
Cited by 17 | Viewed by 3868
Abstract
Recent developments in Distributed Satellite Systems (DSS) have undoubtedly increased mission value due to the ability to reconfigure the spacecraft cluster/formation and incrementally add new or update older satellites in the formation. These features provide inherent benefits, such as increased mission effectiveness, multi-mission [...] Read more.
Recent developments in Distributed Satellite Systems (DSS) have undoubtedly increased mission value due to the ability to reconfigure the spacecraft cluster/formation and incrementally add new or update older satellites in the formation. These features provide inherent benefits, such as increased mission effectiveness, multi-mission capabilities, design flexibility, and so on. Trusted Autonomous Satellite Operation (TASO) are possible owing to the predictive and reactive integrity features offered by Artificial Intelligence (AI), including both on-board satellites and in the ground control segments. To effectively monitor and manage time-critical events such as disaster relief missions, the DSS must be able to reconfigure autonomously. To achieve TASO, the DSS should have reconfiguration capability within the architecture and spacecraft should communicate with each other through an Inter-Satellite Link (ISL). Recent advances in AI, sensing, and computing technologies have resulted in the development of new promising concepts for the safe and efficient operation of the DSS. The combination of these technologies enables trusted autonomy in intelligent DSS (iDSS) operations, allowing for a more responsive and resilient approach to Space Mission Management (SMM) in terms of data collection and processing, especially when using state-of-the-art optical sensors. This research looks into the potential applications of iDSS by proposing a constellation of satellites in Low Earth Orbit (LEO) for near-real-time wildfire management. For spacecraft to continuously monitor Areas of Interest (AOI) in a dynamically changing environment, satellite missions must have extensive coverage, revisit intervals, and reconfiguration capability that iDSS can offer. Our recent work demonstrated the feasibility of AI-based data processing using state-of-the-art on-board astrionics hardware accelerators. Based on these initial results, AI-based software has been successively developed for wildfire detection on-board iDSS satellites. To demonstrate the applicability of the proposed iDSS architecture, simulation case studies are performed considering different geographic locations. Full article
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7 pages, 1007 KiB  
Article
Resazurin Reduction-Based Assays Revisited: Guidelines for Accurate Reporting of Relative Differences on Metabolic Status
by Beatriz Vieira-da-Silva and Miguel A. R. B. Castanho
Molecules 2023, 28(5), 2283; https://doi.org/10.3390/molecules28052283 - 1 Mar 2023
Cited by 23 | Viewed by 6399
Abstract
Cell viability and metabolic activity are ubiquitous parameters used in biochemistry, molecular biology, and biotechnological studies. Virtually all toxicology and pharmacological projects include at some point the evaluation of cell viability and/or metabolic activity. Among the methods used to address cell metabolic activity, [...] Read more.
Cell viability and metabolic activity are ubiquitous parameters used in biochemistry, molecular biology, and biotechnological studies. Virtually all toxicology and pharmacological projects include at some point the evaluation of cell viability and/or metabolic activity. Among the methods used to address cell metabolic activity, resazurin reduction is probably the most common. At variance with resazurin, resorufin is intrinsically fluorescent, which simplifies its detection. Resazurin conversion to resorufin in the presence of cells is used as a reporter of metabolic activity of cells and can be detected by a simple fluorometric assay. UV–Vis absorbance is an alternative technique but is not as sensitive. In contrast to its wide empirical “black box” use, the chemical and cell biology fundamentals of the resazurin assay are underexplored. Resorufin is further converted to other species, which jeopardizes the linearity of the assays, and the interference of extracellular processes has to be accounted for when quantitative bioassays are aimed at. In this work, we revisit the fundamentals of metabolic activity assays based on the reduction of resazurin. Deviation to linearity both in calibration and kinetics, as well as the existence of competing reactions for resazurin and resorufin and their impact on the outcome of the assay, are addressed. In brief, fluorometric ratio assays using low resazurin concentrations obtained from data collected at short time intervals are proposed to ensure reliable conclusions. Full article
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20 pages, 1189 KiB  
Article
GMLM-CNN: A Hybrid Solution to SWIR-VIS Face Verification with Limited Imagery
by Zhicheng Cao, Natalia A. Schmid, Shufen Cao and Liaojun Pang
Sensors 2022, 22(23), 9500; https://doi.org/10.3390/s22239500 - 5 Dec 2022
Cited by 6 | Viewed by 2322
Abstract
Cross-spectral face verification between short-wave infrared (SWIR) and visible light (VIS) face images poses a challenge, which is motivated by various real-world applications such as surveillance at night time or in harsh environments. This paper proposes a hybrid solution that takes advantage of [...] Read more.
Cross-spectral face verification between short-wave infrared (SWIR) and visible light (VIS) face images poses a challenge, which is motivated by various real-world applications such as surveillance at night time or in harsh environments. This paper proposes a hybrid solution that takes advantage of both traditional feature engineering and modern deep learning techniques to overcome the issue of limited imagery as encountered in the SWIR band. Firstly, the paper revisits the theory of measurement levels. Then, two new operators are introduced which act at the nominal and interval levels of measurement and are named the Nominal Measurement Descriptor (NMD) and the Interval Measurement Descriptor (IMD), respectively. A composite operator Gabor Multiple-Level Measurement (GMLM) is further proposed which fuses multiple levels of measurement. Finally, the fused features of GMLM are passed through a succinct and efficient neural network based on PCA. The network selects informative features and also performs the recognition task. The overall framework is named GMLM-CNN. It is compared to both traditional hand-crafted operators as well as recent deep learning-based models that are state-of-the-art, in terms of cross-spectral verification performance. Experiments are conducted on a dataset which comprises frontal VIS and SWIR faces acquired at varying standoffs. Experimental results demonstrate that, in the presence of limited data, the proposed hybrid method GMLM-CNN outperforms all the other methods. Full article
(This article belongs to the Section Sensing and Imaging)
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