Search Results (253)

Table grapes (fresh Vitis vinifera L. fruit) rank among the top five fruit crops worldwide, yet their high perishability poses significant challenges for postharvest handling and storage. This review offers a comprehensive analysis of current and emerging preservation strategies—including chemical fumigation, irradiation, packaging technologies, controlled-atmosphere (CA) storage, biodegradable coatings, and synergistic preservation systems. Distinct from prior studies that typically emphasize specific techniques or treatment categories, this work integrates mechanistic insights with technological advancements and industrial practices across multiple preservation modalities. It further evaluates the comparative effectiveness, limitations, and practical relevance of these strategies along the supply chain. Importantly, it identifies critical research gaps—such as the lack of cultivar-specific preservation protocols, the need for low-residue and environmentally sustainable treatments, and the absence of real-time quality monitoring systems. Addressing these gaps is essential for developing next-generation solutions. Finally, this review highlights practical implications by offering a forward-looking framework to guide innovation, providing grape producers and supply chain stakeholders with strategies to minimize losses, preserve quality, and enhance market competitiveness. Full article

During the automated picking of table grapes, the automatic recognition and segmentation of grape pedicels, along with the positioning of picking points, are vital components for all the following operations of the harvesting robot. In the actual scene of a grape plantation, however, it is extremely difficult to accurately and efficiently identify and segment grape pedicels and then reliably locate the picking points. This is attributable to the low distinguishability between grape pedicels and the surrounding environment such as branches, as well as the impacts of other conditions like weather, lighting, and occlusion, which are coupled with the requirements for model deployment on edge devices with limited computing resources. To address these issues, this study proposes a novel picking point localization method for table grapes based on an instance segmentation network called Progressive Global-Local Structure-Sensitive Segmentation (PGSS-YOLOv11s) and a simple combination strategy of morphological operators. More specifically, the network PGSS-YOLOv11s is composed of an original backbone of the YOLOv11s-seg, a spatial feature aggregation module (SFAM), an adaptive feature fusion module (AFFM), and a detail-enhanced convolutional shared detection head (DE-SCSH). And the PGSS-YOLOv11s have been trained with a new grape segmentation dataset called Grape-⊥, which includes 4455 grape pixel-level instances with the annotation of ⊥-shaped regions. After the PGSS-YOLOv11s segments the ⊥-shaped regions of grapes, some morphological operations such as erosion, dilation, and skeletonization are combined to effectively extract grape pedicels and locate picking points. Finally, several experiments have been conducted to confirm the validity, effectiveness, and superiority of the proposed method. Compared with the other state-of-the-art models, the main metrics $F1$ score and mask mAP@0.5 of the PGSS-YOLOv11s reached 94.6% and 95.2% on the Grape-⊥ dataset, as well as 85.4% and 90.0% on the Winegrape dataset. Multi-scenario tests indicated that the success rate of positioning the picking points reached up to 89.44%. In orchards, real-time tests on the edge device demonstrated the practical performance of our method. Nevertheless, for grapes with short pedicels or occluded pedicels, the designed morphological algorithm exhibited the loss of picking point calculations. In future work, we will enrich the grape dataset by collecting images under different lighting conditions, from various shooting angles, and including more grape varieties to improve the method’s generalization performance. Full article

While resistance or tolerance is a highly sought-after trait in new grape varieties, producing such grapes does not guarantee acceptance in the market. The objective of this study was to explore consumers’ appreciation of a novel tolerant table grape variety by integrating chemical, textural, and sensory analyses with near-infrared (NIR) spectroscopy. To achieve this, we developed multivariate prediction models using artificial neural networks (ANNs) that were applied to the spectra of samples subjected to sensory analysis, allowing us to predict their composition. This approach offers a non-destructive way to conduct sensory analysis, as a single NIR spectrum can assess consumer appreciation and identify the chemical and physical characteristics of each berry. For the grape variety tested, we observed a significant favorable consensus among consumers. Therefore, instead of identifying differences, we focused on determining the optimal maturity ranges that enhance consumer appreciation for this variety. Our findings represent a step toward non-destructive sensory analysis; however, further research is still needed to refine this approach. Full article

Viticulture contributes significantly to Chile’s exports and GDP. However, the development and productivity of grapevines is threatened by climate change. Grapevines are grown in diverse regions; thus, adaptable tools for evaluating climate risk at the local level are required. In this study, a local climate risk index (LCRI) was developed to assess the vulnerability of Chilean viticulture (wine, table, and pisco grapes) in the current (2017–2024) and future (2046–2065) periods. Various components, including exposure, sensitivity, and adaptive and response capacities, were analyzed using different indicators based on municipal-level information. The results for the current period indicated that most municipalities were at medium risk, whereas future projections showed a marked increase in climate risk, principally due to changes in climate suitability. In the current period, the highest LCRI values were observed in semi-arid and mediterranean zones, particularly in the northern regions of Atacama and Coquimbo; in the future period, this situation intensified. In contrast, the lowest values in the current period occurred in the Maule region and further south, where the climate transitions from mediterranean to temperate conditions, and in the future period, valley and mountainous areas presented improvements in the index. Some municipalities showed improvement or stability with local adaptation efforts. The results highlight the urgent need for region-specific adaptation policies that prioritize water management, infrastructure, and increased capacities. Full article

Aroma composition serves as a pivotal quality determinant in table grapes (Vitis vinifera). While the cytochrome P450 enzyme CYP76F14 is implicated in aroma biosynthesis, its functional role in grape berries remains uncharacterized. A comparative analysis of three aroma-distinct cultivars—Muscat type ‘Irsai Oliver’, Neutral type ‘Yanhong’, and Berry-like type ‘Venus Seedless’—revealed cultivar-specific linalool accumulation patterns. ‘Irsai Oliver’ exhibited sustained linalool biosynthesis from the fruit set through to maturity (from Stage 1 to Stage 5), with concentrations peaking at Stage 3 (veraison phase) and remaining elevated until harvest, surpassing the other two cultivars. Transcriptional profiling demonstrated that the CYP76F14 expression exhibited a similar trend with the accumulation of linalool levels, showing a higher expression in ‘Irsai Oliver’ across the developmental stages. A structural analysis identified 12 divergent residues in the ‘Irsai Oliver’ CYP76F14 variant, including E378 and T380 within the conserved substrate recognition site. The site-directed mutagenesis of these residues (CYP76F14-E378G/T380A) reduced the catalytic efficiency by 68–72% compared to the wild-type (in vitro LC-MS/MS assays), confirming their functional significance. This work reveals that cytochrome P450 CYP76F14 mediates the conversion of its substrate linalool in table grape berries, especially of Muscat type grapes, and proposes the CYP76F14 polymorphic variants as molecular markers for aroma-type breeding. The identified catalytic residues (E378/T380) provide targets for enzymatic engineering to modulate the terpenoid profiles in Vitis species. Full article

In plants, ferric-chelate reductase (FRO) plays a critical role in mediating extracellular iron (Fe) reduction, a process essential for cellular Fe homeostasis and abiotic stress tolerance. However, the biological functions and regulatory mechanisms of FRO proteins in fruit crops remain poorly characterized. Here, six VvFRO genes were identified in the table grape cultivar ‘Yanhong’. Transcriptional analysis revealed that root expression of these genes was mainly induced under Fe deficiency, Fe depletion, NaCl stress, and PEG-induced drought stress, respectively, but remained unchanged by low temperature (4 °C) or heat treatment (45 °C). Among them, VvFRO3 exhibited the highest constitutive expression, predominantly in leaves, and was significantly up-regulated under Fe deficiency, Fe depletion, or NaCl treatment. Functional complementation assays demonstrated that heterologous overexpression of VvFRO3 in the Arabidopsis thaliana fro2 knockout mutant rescued its growth retardation phenotype, particularly under Fe-deficient conditions. This study advances our understanding of Fe uptake, transport, and homeostasis mechanisms in perennial fruit crops. Full article

Table grapes are widely cultivated worldwide and are highly appreciated by consumers for their sensory characteristics and high nutritional value. Recently, many researchers have focused on applying specific post-harvest preservation strategies, such as the use of ozone, which represents an environmentally and health-friendly approach to sanitizing and preserving fresh fruits. In addition, ozone acts as a stressor, stimulating the defense mechanism of fruits and the synthesis of polyphenols, important antioxidant compounds. This study aimed to investigate the effect of different concentrations of ozonated water (8 ppm and 12 ppm), cold stored for 12 (T1) and 24 (T2) days, on the weight loss, texture and color parameters, phenolic content, and antioxidant activity of ready-to-eat Italian ‘Regal seedless’ grapes. Weight loss increased during cold storage for all treatments; however, the control exhibited higher values at 12 and 24 days (0.30% and 1.06%, respectively). Cold storage clearly affects the color of grapes, resulting in a loss of brightness and saturation, an increase in the yellow component, and a reduction in hue. At T2, ozonated water showed a significant increase in total phenolic content (TPC), antioxidant activity (DPPH, ORAC), and some phenolic compounds, including gallic acid, kaempferol, and resveratrol, compared to the control. The results indicated that using ozonated water on grape berries could be an effective strategy for enhancing their shelf life and nutraceutical value in post-harvest treatment. Full article

Contamination of agricultural products by Fusarium species is a significant concern and is commonly found in various agricultural products. They cause severe economic losses in the products, and contaminate and threaten human and animal health due to the toxins they produce. Therefore, determining species diversity in various agricultural products is crucial. Bozcaada is well suited for cultivating the highest quality Çavuş grape due to its unique location and climate. Therefore, in this study, the sequencing of the tef1 and tub2 genes in Fusarium isolates from table Çavuş and wine grapes Karalahna, which are specific to Bozcaada, was performed, and their phylogenetic relationships were examined. As a result, it was determined that 11 of the 17 isolates were Fusarium annulatum from the Fusarium fujikuroi species complex (FFSC), 2 were Fusarium nirenbergiae from the Fusarium oxysporum species complex (FOSC), 2 were Fusarium fabacearum from the FOSC, and the last 2 isolates were Fusarium makinsoniae and Fusarium clavus (as ‘clavum’) from the F. incarnatum-equiseti species complex (FIESC). The F. makinsoniae and F. fabacearum species obtained in the study are the first recorded for Türkiye. This research highlights the variety of Fusarium species identified in Bozcaada vineyards in Türkiye. Full article

The bagging technique is a traditional preharvest practice used in Vinalopó Bagged Table Grape production to improve fruit quality and protect clusters from environmental stress. However, its influence on grape volatile composition remains underexplored. This study analyzed the volatile profile of three grape varieties (‘Dominga’, ‘Aledo’, and ‘Doña María’) by comparing bagged and non-bagged clusters to assess the effect of bagging on aromatic compounds. Volatiles were extracted using headspace solid-phase microextraction (HS-SPME) and analyzed by gas chromatography–mass spectrometry (GC–MS). A total of 35 volatile compounds were identified and quantified, mainly aldehydes, terpenes, and alcohols. The highest concentration was found in non-bagged ‘Dominga’ grapes (57.17 mg kg⁻¹), and the lowest in bagged ‘Doña María’ grapes (16.36 mg kg⁻¹). Although total volatile content did not differ significantly between treatments, differences were observed in the relative abundance of chemical families. Bagged grapes showed higher proportions of aldehydes, such as hexanal and (E)-2-hexenal, contributing to green, fresh aromas, while non-bagged grapes exhibited more alcohols and esters, linked to fruity and overripe notes. This study offers new insights into the role of preharvest bagging in shaping grape volatile composition, contributing to a better understanding of its impact on fruit aroma and quality. Full article

The cultivation of table grapes in Brazil is economically significant, with production influenced by edaphoclimatic factors and rootstock selection. The cultivar ‘BRS Núbia’ (Vitis vinifera L. x Vitis labrusca L.) is a promising alternative; however, its phenological behavior, thermal requirements, and compatibility with different rootstocks under subtropical conditions require further evaluation. This study aimed to assess the duration of phenological stages, thermal requirement, and ripening dynamics of ‘BRS Núbia’ grapevines grafted onto the rootstocks ‘IAC 572 Jales’, ‘IAC 766 Campinas’, and ‘Paulsen 1103’. The experiment was conducted in São Manuel, São Paulo, Brazil during the 2021 and 2022 production cycles using a split-plot experimental design (3 × 2). Evaluations included the duration of phenological stages from pruning to budburst, flowering, fruit set, onset of ripening, and harvest, as well as the ripening curve and thermal accumulation from pruning to harvest. Rootstocks did not significantly affect (p > 0.05) the duration of phenological stages; however, differences were observed between production cycles. The 2022 cycle was longer (167.7 days) compared to 2021 (142.6 days), with greater thermal accumulation (1871.7 GDDs vs. 1743.4 GDDs). The analysis of phenological stages revealed that, across both production cycles evaluated, the ‘BRS Núbia’ cultivar required an average accumulation of 1807.5 growing degree days from pruning to harvest. Soluble solids content ranged from 17.43 to 18.50°Brix, and titratable acidity decreased throughout maturation. The maturation index was highest in vines grafted onto ‘Paulsen 1103’, indicating its positive influence on fruit quality. The ‘BRS Núbia’ grapevine exhibited a mean thermal requirement of 1807.5 growing degree days (GDDs) to complete its phenological cycle, which lasted approximately 150 days under subtropical conditions. Full article

Bunch compactness (BC) is a complex, multi-trait characteristic that has been studied mostly in the context of wine grapes, with table grapes being scarcely considered. As these groups have marked phenotypic and genetic differences, including BC, the study of this trait is reported here using a genetically diverse collection of 116 Vitis vinifera L. cultivars and lines enriched for table grapes over two seasons. For this, 3D scanning-based morphological data were combined with ground measurements of 14 BC-related traits, observing high correlations among both approaches (R² > 0.90–0.97). The multivariate analysis suggests that the attributes ‘berries per bunch’, ‘berry weight and width’, and ‘bunch weight and length’ could be considered as the main descriptors for BC, optimizing evaluation times. Then, GWASs based on a set of 70,335 SNPs revealed that GBS analysis in this same population enabled the detection of several SNPs associated with different sub-traits, with a locus for ‘berries per bunch’ in chromosome (chr) 18 being the most prominent. Enrichment analysis of significant and frequent SNPs found simultaneously in several traits and seasons revealed the over-representation of discrete functions such as alpha-linolenic acid metabolism and glycan degradation. In summary, the utility of 3D automated phenotyping was validated for table grape backgrounds, and new SNPs and candidate genes associated with the BC trait were detected. The latter could eventually become a selection tool for grapevine breeding programs. Full article

Grape cultivation is crucial due to its significant dietary benefits and the production of various byproducts. Fungicides, like boscalid, are frequently applied to protect grape crops from several disease, ensuring both yield and quality. However, the excessive or inappropriate application of boscalid may pose health risks to humans. Therefore, the objectives of this field study were (a) to assess the pre-harvest interval (PHI) and dissipation of boscalid in two table-grape varieties (Soultanina and Crimson) under field conditions and (b) evaluate the potential health risks associated with grape consumption for both adults and children. The residue of boscalid in the grapes was determined using a modified QuEChERS method coupled with a high-performance liquid chromatography diode array detector (HPLC–DAD). The dissipation of boscalid followed first- and second-order kinetics, with half-lives ranging from 3.32 to 6.42 days and PHIs from 8.11 to 10.90 days. The dietary risk assessment indicated that the early to mid-post application period could pose risks for both age groups, with children facing three times the risk of adults. Full article

Grapes, one of the most delicious and refreshing fruits in the world, are a source of sugar, minerals, and vitamins. Summer pruning affects ripening, disease control, yield, and quality parameters by controlling the vine microclimate. In our study, leaf removal, fruit thinning, and cluster thinning and their combination were applied to the Alphonse Lavallée grape variety, aiming to improve yield, cluster, and berry characteristics. As a result of the applications, cluster and berry characteristics, SSC, pH, titratable acidity (TA), total phenolic content, antioxidant activity, and color parameters were examined. In our study, all summer pruning applications and their combinations caused increases in cluster and berry parameters (weight, length, and width) compared to the control. In addition to these, the SSC, pH, and maturity index increased and TA decreased. All these applications also increased berry detachment and skin rupture force, which have an important place in road resistance in table grape varieties. Significant improvements were also seen in the quality parameters of total phenolic content and antioxidant activity. In addition, there were increases in the lightness and chroma values that determine the fruit quality in table grapes. Considering all these data, the summer pruning applications we made had significant effects on yield and quality. It is thought that cutting a part of the clusters instead of the whole cluster will especially prevent the yield loss experienced in cluster thinning applications. Full article

(1) Background: The variability and trend in harvest dates of table and Pisco grapes have been scarcely studied. This can be closely influenced by bioclimatic indices since they account for the interactions between climatic factors and vine phenology. Understanding the environmental factors influencing harvest timing has become increasingly critical to perform specific viticultural practices. (2) Methods: The aim of this research was to evaluate the influence of bioclimatic indices on variability and trend of harvest date from the 2002–2003 to 2017–2018 seasons in Flame Seedless, Thompson Seedless, Muscat of Alexandria, and Moscatel Rosada growing in Northern Chile. (3) Results: The harvest date of Flame Seedless advanced significantly with an increasing Growing Season Temperature (GST) (from 1 October to 31 December), while Thompson Seedless showed a significant advancement in harvest date with rising the Maximum Springtime Temperature Summation SON_max (from 1 September to 30 November) values. Similarly, the harvest date of Muscat of Alexandria was significantly earlier with higher Heliothermal Index (HI) (from 1 July to 31 January and from 1 August to 30 April) values, whereas Moscatel Rosada exhibited a significant advancement in harvest date as the GST (from 1 July to 31 December and from 1 July to 31 January) increased. The trend in the harvest date of Thompson Seedless was statistically significant, reaching a coefficient of determination of 0.42. (4) Conclusions: Understanding the influence of bioclimatic indices on harvest date in long-term periods is critical in the context of climatic variability since producers can make more informed decisions to optimize grape quality and maintain sustainability in production systems. Full article

The endophytic strain Amfr20 was isolated from roots of the olive tree var. Amfissa. Based on core-genome phylogenomic analyses, it was classified as Bacillus velezensis. The isolate showed positive results in numerous plant growth promoting traits, as well as in abiotic stress tolerance and in colonization related traits in vitro. Furthermore, the strain exhibited antifungal activity in vitro through diffusible and volatile compounds. Whole genome analysis revealed that the strain possesses large and various arsenals of secondary metabolite biosynthetic gene clusters involved in the bioagent’s functional properties, including plant growth promotion, colonization, and plant defense elicitation, as well as having the genomic potential for abiotic stress mediation. Based on TLC-bioautography, the ethyl acetate extracts of secreted agar-diffusible compounds from Amfr20 through single and dual cultures were found to be bioactive independently of the fungal pathogen’s interaction. The bacterial endophyte also proved efficient in suppressing the severity of anthracnose olive rot and gray mold post-harvest diseases on olive fruits and table grape berries, respectively. Lastly, Amfr20 beneficially affected Arabidopsis thaliana growth under normal and saline conditions, while boosting the plant development of Solanum lycopersicum through seed biopriming and root irrigation methods. The results of this multilevel study indicate that the novel endophyte Amfr20 Bacillus velezensis is a promising bioagent that should be exploited in the future as an ecological biopesticide and/or biostimulant. Full article