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Keywords = three-level T-type inverter

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30 pages, 5764 KB  
Article
Control and Modeling Framework for Balanced Operation and Electro-Thermal Analysis in Three-Level T-Type Neutral Point Clamped Inverters
by Ahmed H. Okilly, Cheolgyu Kim, Do-Wan Kim and Jeihoon Baek
Energies 2025, 18(21), 5587; https://doi.org/10.3390/en18215587 - 24 Oct 2025
Viewed by 768
Abstract
Reliable multilevel inverter IGBT modules require precise loss and heat management, particularly in severe traction applications. This paper presents a comprehensive modeling framework for three-level T-type neutral-point clamped (TNPC) inverters using a high-power Insulated Gate Bipolar Transistor (IGBT) module that combines model predictive [...] Read more.
Reliable multilevel inverter IGBT modules require precise loss and heat management, particularly in severe traction applications. This paper presents a comprehensive modeling framework for three-level T-type neutral-point clamped (TNPC) inverters using a high-power Insulated Gate Bipolar Transistor (IGBT) module that combines model predictive control (MPC) with space vector pulse width modulation (SVPWM). The particle swarm optimization (PSO) algorithm is used to methodically tune the MPC cost function weights for minimization, while achieving a balance between output current tracking, stabilization of the neutral-point voltage, and, consequently, a uniform distribution of thermal stress. The proposed SVPWM-MPC algorithm selects optimal switching states, which are then utilized in a chip-level loss model coupled with a Cauer RC thermal network to predict transient chip-level junction temperatures dynamically. The proposed framework is executed in MATLAB R2024b and validated with experiments, and the SemiSel industrial thermal simulation tool, demonstrating both control effectiveness and accuracy of the electro-thermal model. The results demonstrate that the proposed control method can sustain neutral-point voltage imbalance of less than 0.45% when operating at 25% load and approximately 1% under full load working conditions, while accomplishing a uniform junction temperature profile in all inverter legs across different working conditions. Moreover, the results indicate that the proposed control and modeling structure is an effective and common-sense way to perform coordinated electrical and thermal management, effectively allowing for predesign and reliability testing of high-power TNPC inverters. Full article
(This article belongs to the Special Issue Power Electronics Technology and Application)
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20 pages, 5833 KB  
Article
Power Factor Adaptive DPWM Control Strategy for T-Type Three-Level Inverters
by Jialiang Tian, Yingying Xu, Mingxia Xu, Zhenjiang Liu and Yuchi Zhou
Energies 2025, 18(17), 4574; https://doi.org/10.3390/en18174574 - 28 Aug 2025
Cited by 1 | Viewed by 1269
Abstract
With the widespread application of multilevel inverters, device losses have become a critical area of research. A key limitation of conventional three-level discontinuous pulse width modulation (DPWM) strategies is their inability to maintain switching device clamping during the peak intervals of the load [...] Read more.
With the widespread application of multilevel inverters, device losses have become a critical area of research. A key limitation of conventional three-level discontinuous pulse width modulation (DPWM) strategies is their inability to maintain switching device clamping during the peak intervals of the load current, especially under varying load power factor conditions, thereby reducing switching losses. This paper proposes an improved three-level power factor adaptive DPWM (PFA-DPWM) strategy that minimizes switching losses by clamping the power devices during the one-third fundamental period of maximum load current. First, a unified mathematical model of DPWM strategies is established. Theoretical analysis demonstrates that phase disposition (PD) carrier modulation for three-level inverter exhibits superior line voltage harmonic characteristics. Based on this, a theoretical comparison of switching losses and harmonic distortion for various DPWM schemes is conducted. The proposed PFA-DPWM control strategy has the minimum switching loss without compromising harmonic performance. The efficacy and validity of the proposed strategy are confirmed by comprehensive simulation and experimental results. Full article
(This article belongs to the Special Issue Advanced Power Electronics Technology: 2nd Edition)
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15 pages, 4292 KB  
Article
Research on Medium Voltage Energy Storage Inverter Control Based on Hybrid Variable Virtual Vectors
by Zhimin Mei, Kai Xiong and Jiang Liu
Electronics 2025, 14(17), 3372; https://doi.org/10.3390/electronics14173372 - 25 Aug 2025
Viewed by 742
Abstract
Medium-voltage energy storage converter equipment is an important component of the new generation of ship power and power systems. Virtual space vector pulse width modulation, as a modulation optimization method to improve the neutral-point voltage imbalance in medium- and high-voltage multilevel energy storage [...] Read more.
Medium-voltage energy storage converter equipment is an important component of the new generation of ship power and power systems. Virtual space vector pulse width modulation, as a modulation optimization method to improve the neutral-point voltage imbalance in medium- and high-voltage multilevel energy storage converters, has become a research hotspot for T-type three-level energy storage inverter modulation methods due to its significant balancing effect and simple implementation. However, the current research method of constructing virtual vectors through redundant small vectors has limitations in regulating the neutral-point potential under full (especially high) modulation ratios. This paper proposes a modulation method that uses hybrid variable virtual small vectors and virtual medium vectors through optimization selection and reconstruction of basic vectors. This method ensures that the neutral-point charge change of the vector is zero and the common-mode voltage is minimized within the switching period under the full modulation ratio, achieving the purpose of controlling the neutral-point voltage balance and suppressing the common-mode voltage. Finally, simulation and experimental results show that the proposed method has good neutral-point voltage regulation and common-mode voltage suppression capabilities within the full modulation ratio range, and the system also has strong robustness and adaptability under different load conditions. Full article
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14 pages, 2673 KB  
Article
Evaluation of GaN Transistors for Grid-Connected 3-Level T-Type Inverters
by Julian Endres, Tobias Haas, Alexander Pawellek, Vinicius Kremer and Roger Franchino
Electronics 2025, 14(15), 2935; https://doi.org/10.3390/electronics14152935 - 23 Jul 2025
Cited by 2 | Viewed by 991
Abstract
This paper presents a complete workflow for the evaluation of GaN transistors in voltage source inverters. With the associated high switching speed of transistors based on GaN, it is important to consider some critical points in the design phase as well as in [...] Read more.
This paper presents a complete workflow for the evaluation of GaN transistors in voltage source inverters. With the associated high switching speed of transistors based on GaN, it is important to consider some critical points in the design phase as well as in the measurement setup in order to be able to utilise and verify the advantages of GaN properly. For this reason, the presented circuit board’s design focuses on a minimised power loop inductance. Simulation models, an analytical approach and measurement results with the aim of determining this inductance are compared with each other. A good compliance results between the presented methods. Additionally, the description of a test bench is given, which enables the performance of the opposition method. This setup allows the measurement of the designed H-bridge’s arising losses and the GaN-transistor’s switching behaviour. In comparison to the conventional double pulse method, this approach enables results that are more accurate for determining losses. Full article
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30 pages, 3950 KB  
Article
Estimation of Peak Junction Hotspot Temperature in Three-Level TNPC-IGBT Modules for Traction Inverters Through Chip-Level Modeling and Experimental Validation
by Ahmed H. Okilly, Peter Nkwocha Harmony, Cheolgyu Kim, Do-Wan Kim and Jeihoon Baek
Energies 2025, 18(14), 3829; https://doi.org/10.3390/en18143829 - 18 Jul 2025
Cited by 2 | Viewed by 1341
Abstract
Monitoring the peak junction hotspot temperature in IGBT modules is critical for ensuring the reliability of high-power industrial multilevel inverters, particularly when operating under extreme thermal conditions, such as in traction applications. This study presents a comprehensive chip-level analytical loss and thermal model [...] Read more.
Monitoring the peak junction hotspot temperature in IGBT modules is critical for ensuring the reliability of high-power industrial multilevel inverters, particularly when operating under extreme thermal conditions, such as in traction applications. This study presents a comprehensive chip-level analytical loss and thermal model for estimation of the peak junction hotspot temperature in a three-level T-type neutral-point-clamped (TNPC) IGBT module. The developed model includes a detailed analytical assessment of conduction and switching losses, along with transient thermal network modeling, based on the actual electrical and thermal characteristics of the IGBT module. Additionally, a hybrid thermal–electrical stress experimental setup, designed to replicate real operating conditions, was implemented for a balanced three-phase inverter circuit utilizing a Semikron three-level IGBT module, with testing currents reaching 100 A and a critical case temperature of 125 °C. The analytically estimated module losses and peak junction hotspot temperatures were validated through direct experimental measurements. Furthermore, thermal simulations were conducted with Semikron’s SemiSel benchmark tool to cross-validate the accuracy of the thermo-electrical model. The outcomes show a relative estimation error of less than 1% when compared to experimental data and approximately 1.15% for the analytical model. These findings confirm the model’s accuracy and enhance the reliability evaluation of TNPC-IGBT modules in extreme thermal environments. Full article
(This article belongs to the Special Issue Power Electronics Technology and Application)
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23 pages, 6307 KB  
Article
Enhanced Sliding Mode Control for Dual MPPT Systems Integrated with Three-Level T-Type PV Inverters
by Farzaneh Bagheri, Jakson Bonaldo, Naki Guler, Marco Rivera, Patrick Wheeler and Rogerio Lima
Energies 2025, 18(13), 3344; https://doi.org/10.3390/en18133344 - 26 Jun 2025
Cited by 1 | Viewed by 1246
Abstract
Dual Maximum Power Point Tracking (MPPT) inverters are essential in residential and small commercial solar power systems, optimizing power extraction from two independent solar panel arrays to enhance efficiency and energy harvesting. On the other hand, the Three-Level T-Type Voltage Source Inverter (3L [...] Read more.
Dual Maximum Power Point Tracking (MPPT) inverters are essential in residential and small commercial solar power systems, optimizing power extraction from two independent solar panel arrays to enhance efficiency and energy harvesting. On the other hand, the Three-Level T-Type Voltage Source Inverter (3L T-Type VSI) is known for its reduced switching losses, improved harmonic distortion, and reduced part count in comparison to other three-level topologies. In this paper, a novel architecture is proposed to integrate the dual MPPT structure directly to each DC-side split capacitor of the 3L T-Type VSI, taking advantage of the intrinsic characteristics of the inverter’s topology. Further performance enhancement is achieved by integrating a classical MPPT strategy to the control framework to make it feasible for a real-case grid integration. The combination of these methods ensures faster and stable tracking under dynamic irradiance conditions. Considering that strategies dedicated to balancing the DC-link capacitor’s voltage slightly affect the AC-side current waveform, an enhanced sliding mode control (SMC) strategy tailored for dual MPPT and 3L T-Type VSI is deployed, combining the simplicity of conventional PI controllers used in the independent MPPT-based DC-DC converters with the superior robustness and dynamic performance of SMC. Real-time results obtained using the OPAL-RT Hardware-in-the-Loop platform validated the performance of the proposed control strategy under realistic test scenarios. The current THD was maintained below 4.8% even under highly distorted grid conditions, and the controller achieved a steady state within approximately 15 ms following perturbations in the DC-link voltage, sudden irradiance variations, and voltage sags and swells. Additionally, the power factor remained unitary, enhancing power transfer from the renewable source to the grid. The proposed system was able to achieve efficient power extraction while maintaining high power quality (PQ) standards for the output, positioning it as a practical and flexible solution for advanced solar PV systems. Full article
(This article belongs to the Section A2: Solar Energy and Photovoltaic Systems)
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22 pages, 2440 KB  
Article
Open-Circuit Fault Diagnosis for T-Type Three-Level Inverter via Improved Adaptive Threshold Sliding Mode Observer
by Xiaoyan Zhang, Ziyan Shang, Song Gao, Suping Zhao, Chaobo Chen and Kun Wang
Appl. Sci. 2025, 15(11), 6063; https://doi.org/10.3390/app15116063 - 28 May 2025
Cited by 1 | Viewed by 1384
Abstract
T-type three-level inverters have been extensively utilized in renewable energy generation, motor drive systems, and other power conversion applications. However, failures in semiconductor devices critically reduce the operational reliability of power conversion systems. While significant progress has been made in the diagnosis of [...] Read more.
T-type three-level inverters have been extensively utilized in renewable energy generation, motor drive systems, and other power conversion applications. However, failures in semiconductor devices critically reduce the operational reliability of power conversion systems. While significant progress has been made in the diagnosis of single-switch open-circuit (OC) faults, the precise location and detection of simultaneous double-switch OC faults remain challenging. Therefore, this paper proposes a fault diagnosis method, integrating an improved adaptive sliding mode observer (IASMO) and dynamic current threshold detection. First, the IASMO is constructed through the hybrid logic dynamic model, achieving accurate and rapid estimation of phase currents. Then, integrating estimated with actual currents accomplishes the design of detection variables and adaptive thresholds. Subsequently, fault location variables are formulated to achieve accurate localization of both single-switch and double-switch faults. Finally, Simulation and experimental results demonstrate that the proposed method effectively identifies 18 types of OC faults within 75% of the current cycle, with high efficiency and robustness. Full article
(This article belongs to the Section Electrical, Electronics and Communications Engineering)
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36 pages, 2975 KB  
Review
A Review of Hybrid Three-Level ANPC Inverters: Topologies, Comparison, Challenges and Improvements in Applications
by Xiaobin Mu, Hao Chen, Xiang Wang, Weimin Wu, Houqing Wang, Liang Yuan, Henry Shu-Hung Chung and Frede Blaabjerg
Energies 2025, 18(10), 2613; https://doi.org/10.3390/en18102613 - 19 May 2025
Cited by 4 | Viewed by 4713
Abstract
Considering the cost, efficiency, power density, and other issues of the power electronic system, many papers have mixed the wide-bandgap (WBG) power devices, mainly SiC MOSFET and GaN FET/HEMT, with Si IGBT/MOSFET in the three-level active neutral-point clamped (T-ANPC) topology, forming the hybrid [...] Read more.
Considering the cost, efficiency, power density, and other issues of the power electronic system, many papers have mixed the wide-bandgap (WBG) power devices, mainly SiC MOSFET and GaN FET/HEMT, with Si IGBT/MOSFET in the three-level active neutral-point clamped (T-ANPC) topology, forming the hybrid T-ANPC (HT-ANPC) topology. This paper reviews these latest HT-ANPC topologies from the perspective of the material types of switching devices and compares the advantages and disadvantages of various topologies. The potential challenges of HT-ANPC inverters in several mainstream applications are reviewed, and their improvements are compared and discussed in detail. Next, a brief topology selection and design process are provided based on analyzing various typical topologies. In addition, some future research trends on this topic are discussed. The paper will help researchers to select appropriate HT-ANPC topologies in different applications and have a better understanding of the critical issues to be considered during system design. Full article
(This article belongs to the Section F3: Power Electronics)
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18 pages, 6412 KB  
Article
Dual-Vector Model Predictive Current Control with Entire-Time-Domain Current Harmonic Optimization and Robust Control Strategy
by Dahui Guo, Jian Yang, Hanbin Zhou, Qian Huang, Mi Dong and Liqing Liao
Energies 2025, 18(5), 1117; https://doi.org/10.3390/en18051117 - 25 Feb 2025
Viewed by 953
Abstract
This article proposes a robust dual-vector model predictive control (RDVMPC) strategy for high-power three-level inverters, specifically designed to optimize current harmonics. By extending the concept of total harmonic distortion (THD) to the entire time domain, the current deviation is redefined, and a novel [...] Read more.
This article proposes a robust dual-vector model predictive control (RDVMPC) strategy for high-power three-level inverters, specifically designed to optimize current harmonics. By extending the concept of total harmonic distortion (THD) to the entire time domain, the current deviation is redefined, and a novel cost function is formulated. The proposed strategy incorporates dual-vector control to maintain a fixed switching frequency while optimizing the sequences and duty cycles of the vectors to further mitigate THD. A novel ultra-local model is employed to eliminate dependency on precise system parameters, with unknown components accurately estimated through an extended state observer (ESO) equipped with an adaptive bandwidth gain mechanism. This mechanism dynamically adjusts to enhance disturbance rejection under parameter variations and suppress noise under steady-state conditions. The efficacy of the proposed method is substantiated through simulations and experimental validation on a 10 kW T-type inverter, demonstrating significant improvements in harmonic reduction and overall system performance. Full article
(This article belongs to the Section F3: Power Electronics)
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17 pages, 8581 KB  
Article
Enhanced Control Strategy for Three-Level T-Type Converters in Hybrid Power-to-X Systems
by Moria Sassonker Elkayam and Dmitri Vinnikov
Appl. Sci. 2025, 15(5), 2409; https://doi.org/10.3390/app15052409 - 24 Feb 2025
Viewed by 1807
Abstract
This paper presents a dual-loop control system designed for three-level three-phase T-type converters, optimizing their performance in the hybrid operation of Power-to-X systems. Due to the increasing of distributed power generation based on renewable energy sources, Power-to-X systems convert surplus renewable energy into [...] Read more.
This paper presents a dual-loop control system designed for three-level three-phase T-type converters, optimizing their performance in the hybrid operation of Power-to-X systems. Due to the increasing of distributed power generation based on renewable energy sources, Power-to-X systems convert surplus renewable energy into other forms of energy, such as hydrogen, synthetic fuels, or chemical storage, which can be stored and later converted back to electricity or used in other applications. Bidirectional converters play a crucial role in hybrid system operation, which requires an efficient and reliable power conversion to maintain stability and performance. The proposed dual-loop control system includes an inner current loop for fast current regulation and an outer voltage loop to maintain stable voltage levels, ensuring precise control of the output of the converter and enhancing its response to dynamic changes in load and generation. Additionally, the control system incorporates a technique to balance the split DC-link capacitors voltages, a major challenge in three-level converters. Comprehensive simulation and experimental results demonstrate the efficacy of the proposed control system in maintaining high power quality and supporting the hybrid operation of Power-to-X systems. Full article
(This article belongs to the Special Issue Control of Power Systems, 2nd Edition)
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14 pages, 10504 KB  
Article
High-Switching-Frequency SiC Power Conversion Systems with Improved Finite Control Set Method Prediction Control
by Yibiao Fan, Lixia Tong, Yingjie Lu, Xiaowei Cai, Zhihe Fu and Xingkui Mao
Energies 2024, 17(18), 4601; https://doi.org/10.3390/en17184601 - 13 Sep 2024
Cited by 1 | Viewed by 1746
Abstract
With the development of power conversion systems or bidirectional grid-connected inverters characterized by high DC voltage, high efficiency, and high-power density, high-switching-frequency SiC power switches are being widely used, and these require a short computational time of control algorithm. Based on the sector [...] Read more.
With the development of power conversion systems or bidirectional grid-connected inverters characterized by high DC voltage, high efficiency, and high-power density, high-switching-frequency SiC power switches are being widely used, and these require a short computational time of control algorithm. Based on the sector judgment of a space voltage vector and the midpoint potential control balancing of a DC bus, an improved finite control set method prediction control (FCS-MPC) method was proposed for a T-type three-level PCS. This improved FCS-MPC first judges the sector of the space voltage vector and locates the position of the corresponding large sector according to phase lock information; then, it analyzes the sampled voltage of the upper and lower bus capacitors to obtain the midpoint potential situation and selects appropriate small vectors based on the midpoint potential situation. This simple improved strategy can reduce the computational complexity of traditional MPC for rolling optimization, resulting in a reduction in the vectors from 27 to 8. A SiC-based 10 kW bidirectional PCS prototype verified the improved FCS-MPC, and the computational time was reduced about by 56% in comparison to traditional FCS-MPC. Full article
(This article belongs to the Section F3: Power Electronics)
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21 pages, 12225 KB  
Article
A Modified SVPWM Strategy for Reducing PWM Voltage Noise and Balancing Neutral Point Potential
by Renxi Gong, Hao Wu, Jing Tang and Xingyuan Wan
Electronics 2024, 13(9), 1656; https://doi.org/10.3390/electronics13091656 - 25 Apr 2024
Cited by 2 | Viewed by 2812
Abstract
PWM (pulse width modulation) is the most widely applied current conversion technology, but the high-frequency harmonics it causes have a significant negative impact on inverter system performance. This paper focuses on the three-phase T-type three-level inverter as the research object and addresses existing [...] Read more.
PWM (pulse width modulation) is the most widely applied current conversion technology, but the high-frequency harmonics it causes have a significant negative impact on inverter system performance. This paper focuses on the three-phase T-type three-level inverter as the research object and addresses existing PWM voltage noise and midpoint potential imbalance issues by proposing an improved random SVPWM strategy, named Neutral Point Potential Balance Random Space Vector PWM (NPB–RSVPWM). The NPB–RSVPWM strategy includes three main steps: (1) introducing a midpoint potential balancing control loop to adjust the synthesis timing of the effective vectors to generate pulse signals, optimizing midpoint potential balance; (2) employing a randomly varying carrier frequency in place of the carrier used in the SVPWM strategy to generate the driving signals for switching devices; and (3) controlling the inverter through the driving pulse signals. This strategy optimizes the synthesis sequence of traditional SVPWM strategy vectors and incorporates random frequency modulation techniques. The mathematical model analyzes PWM harmonic expressions corresponding to fixed switching frequencies, and a random frequency carrier is chosen to suppress these PWM harmonics. The effective vector’s equivalent circuit is analyzed, proposing a technique for optimized vector synthesis timing. The simulation and experimental results verify that the NPB–RSVPWM technique can disperse PWM harmonic energy, reduce voltage noise, and optimize midpoint potential balance. Under the NPB–RSVPWM strategy, the line voltage spectrum becomes uniform, the maximum harmonic content is greatly reduced, and the fluctuation in the DC side midpoint potential is significantly improved. Compared with the traditional SVPWM strategy and random PWM strategy, the NPB–RSVPWM strategy has a lower voltage noise, smaller total harmonic distortion, and a more stable midpoint potential. The effectiveness and feasibility of the NPB–RSVPWM strategy are verified by simulation and experimental results. Full article
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22 pages, 13534 KB  
Article
Open-Circuit Fault Diagnosis of T-Type Three-Level Inverter Based on Knowledge Reduction
by Xiaojuan Chen and Zhaohua Zhang
Sensors 2024, 24(3), 1028; https://doi.org/10.3390/s24031028 - 5 Feb 2024
Cited by 6 | Viewed by 3228
Abstract
Compared with traditional two-level inverters, multilevel inverters have many solid-state switches and complex composition methods. Therefore, diagnosing and treating inverter faults is a prerequisite for the reliable and efficient operation of the inverter. Based on the idea of intelligent complementary fusion, this paper [...] Read more.
Compared with traditional two-level inverters, multilevel inverters have many solid-state switches and complex composition methods. Therefore, diagnosing and treating inverter faults is a prerequisite for the reliable and efficient operation of the inverter. Based on the idea of intelligent complementary fusion, this paper combines the genetic algorithm–binary granulation matrix knowledge-reduction method with the extreme learning machine network to propose a fault-diagnosis method for multi-tube open-circuit faults in T-type three-level inverters. First, the fault characteristics of power devices at different locations of T-type three-level inverters are analyzed, and the inverter output power and its harmonic components are extracted as the basis for power device fault diagnosis. Second, the genetic algorithm–binary granularity matrix knowledge-reduction method is used for optimization to obtain the minimum attribute set required to distinguish the state transitions in various fault cases. Finally, the kernel attribute set is utilized to construct extreme learning machine subclassifiers with corresponding granularity. The experimental results show that the classification accuracy after attribute reduction is higher than that of all subclassifiers under different attribute sets, reflecting the advantages of attribute reduction and the complementarity of different intelligent diagnosis methods, which have stronger fault-diagnosis accuracy and generalization ability compared with the existing methods and provides a new way for hybrid intelligent diagnosis. Full article
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22 pages, 7641 KB  
Article
Power Loss Modelling and Performance Comparison of Three-Level GaN-Based Inverters Used for Electric Traction
by Arjun Sujeeth, Angelo Di Cataldo, Luigi Danilo Tornello, Mario Pulvirenti, Luciano Salvo, Angelo Giuseppe Sciacca, Giacomo Scelba and Mario Cacciato
Energies 2024, 17(3), 595; https://doi.org/10.3390/en17030595 - 26 Jan 2024
Cited by 13 | Viewed by 3746
Abstract
The main aim of this work is to present a step-by-step procedure to model and analyze the power loss distribution of three-level Gallium Nitride (GaN) inverters. It has been applied to three distinct three-phase three-level voltage source inverters utilized in electric traction drives: [...] Read more.
The main aim of this work is to present a step-by-step procedure to model and analyze the power loss distribution of three-level Gallium Nitride (GaN) inverters. It has been applied to three distinct three-phase three-level voltage source inverters utilized in electric traction drives: Active Neutral Point Clamped, Neutral Point Clamped and T-Type Neutral Point Clamped. The proposed analytical power loss modelling, combined with an equivalent representation of the electrical machine proved to be a viable solution to achieve a time-saving and low computational burden simulation platform, leading to satisfying accurate results. This has been confirmed by comparing the results carried out from the simulations of a 110 kW permanent magnet synchronous motor drive and those determined by considering a simplified circuital representation based on the proposed analytical power loss modelling. Full article
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12 pages, 4297 KB  
Article
Complete Chloroplast Genome Structural Characterization and Comparative Analysis of Viburnum japonicum (Adoxaceae)
by Hong Zhu, Juan Liu, Hepeng Li, Chunlei Yue and Meirong Gao
Forests 2023, 14(9), 1819; https://doi.org/10.3390/f14091819 - 6 Sep 2023
Cited by 5 | Viewed by 2173
Abstract
Viburnum japonicum (Thunb.) Sprengel is an endangered species endemic to coastal regions of eastern Asia (China, Japan, and South Korea). However, its systematic position has been controversial. In this study, we present the complete chloroplast (cp) genome of V. japonicum (GenBank OP644292) sequenced [...] Read more.
Viburnum japonicum (Thunb.) Sprengel is an endangered species endemic to coastal regions of eastern Asia (China, Japan, and South Korea). However, its systematic position has been controversial. In this study, we present the complete chloroplast (cp) genome of V. japonicum (GenBank OP644292) sequenced using the Illumina NovaSeq platform. The cp genome has a total length of 158,606 bp and a G+C contents of 38.08%. It consists of a large single-copy (LSC) region, a small single-copy (SSC) region, and a pair of inverted repeats (IRs) regions measuring 87,060 bp, 18,510 bp, and 26,516 bp, respectively. A total of 131 genes were identified, including 87 protein-coding genes, 36 transfer RNA genes (tRNAs), and 8 ribosomal RNA genes(rRNAs). Additionally, a total of 44 dispersed repeats were detected, including three types: forward, palindromic, and reverse. Among the 38 SSR loci that were discovered, the majority were mononucleotide loci composed of A/T. Furthermore, we found that 15 genes (accD, atpF, ndhA, ndhB, petB, petD, rpl16, rpl2, rpoC1, rps12, rps16, trnA-UGC, trnI, trnK-UUU, and trnL-UAA) contain one intron, while clpP and ycf3 have two introns. The relative synonymous codon usage (RSCU) analysis detected 31 high-frequency codons, where A/U bases accounted for 93.55% of the total, indicating an asymmetry in chloroplast gene and a presence for A/U bases. Comparative analysis of genome structure and sequences data of V. japonicum chloroplast genomes in comparison with other closely related species demonstrated a high level of conservation in their structure and organization. Furthermore, three mutation hotspots (psbH, rps19, and trnL) were identified, which could be valuable for future phylogenetic and population genetic research. Phylogenetic analysis revealed that the two accessions of V. japonicum are closely related to a group of V. setigerum, V. erosum, and V. fordiae within the Viburnum genus. In conclusion, this study provides important insights for accurately identifying and understanding the phylogeny of Viburnum species through the complete cp genome sequencing of V. japonicum. Full article
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