Label-Free Proteome Profiling

Dear Colleagues,

The accurate determination of quantitative changes describing biological systems has attracted considerable interest from the scientific community. Biological processes are regulated through the abundance of proteins. In addition, the relative abundance of each protein in a complex system may influence the modulation of its activity and function. Over the past decade, mass-spectrometry-based proteomics has become an indispensable tool in deciphering the exact abundance of each protein in composite mixtures. Indeed, the fast development of mass-spectrometry-based proteomic technologies and the accessibility of powerful data analysis tools have increasingly boosted the transition of proteomic analysis from qualitative to quantitative, with a strong impact on the biological interpretation of protein functions. The described technological improvement, such as the advent of new high-resolution instruments, has allowed us to overcome classical, label-based quantitative approaches and use proteomics to move toward label-free techniques.

This Topical Collection invites original and interesting contributions of quantitative proteomic studies and aims to describe the translational applicability of label-free proteomic profiling in a wide range of scientific fields. However, the Topical Collection is also open to discussions about the methodological progress linked to quantitative proteomics to facilitate the sharing of experimental and technical details in terms of methodological conditions and bioinformatic analysis.

Dr. Marianna Caterino
Dr. Alessio Soggiu
Topic Editors