Glutathione peroxidases (GPX) catalyze the reduction of H
2O
2 or organic hydroperoxides to water or corresponding alcohols using reduced glutathione, which plays an essential role in ROS (reactive oxygen species) homeostasis and stress signaling.
Thellungiella salsuginea (
Eutrema salsugineum), a
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Glutathione peroxidases (GPX) catalyze the reduction of H
2O
2 or organic hydroperoxides to water or corresponding alcohols using reduced glutathione, which plays an essential role in ROS (reactive oxygen species) homeostasis and stress signaling.
Thellungiella salsuginea (
Eutrema salsugineum), a relative of
Arabidopsis thaliana, displays an extremely high level of tolerance to salt, drought, cold and oxidative stresses. The enzymatic antioxidant systems may contribute to the stress tolerance of
T. salsuginea. In the present study, we aimed at understanding the roles of the antioxidant enzymes in
T. salsuginea by focusing on the GPX family. We identified the eight
GPX genes in
T. salsuginea, and the structure of the
N-terminal domains indicated their putative chloroplastic, mitochondrial and cytoplasmic location. The exon-intron organization of these genes exhibited a conserved pattern among plant
GPX genes. Multiple environmental stresses and hormone response related
cis-acting elements were predicted in the promoters of
TsGPX genes. The gene and protein expression profiles of TsGPXs in response to high level of salinity and osmotic stresses, in leaves and roots of
T. salsuginea were investigated using real-time RT-PCR and western blotting analysis. Our result showed that different members of the
GPX gene family were coordinately regulated under specific environmental stress conditions, and supported the important roles of TsGPXs in salt and drought stress response in
T. salsuginea.
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